CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
mitochondrion 3
cytosol 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89827
KRG90331
KRG94459
KRG96637
KRG99486
KRH02873
KRH06999
KRH09738
KRH44443
KRH47463
KRH51589
KRH60909
KRH68370

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G50590.1	KRH09738	AT1G15370.1	24529374
AT3G50590.1	KRH44443	AT1G15370.1	24529374
AT3G50590.1	KRH47463	AT1G15370.1	24529374
AT3G50590.1	KRH51589	AT1G20760.1	24529374
AT3G50590.1	KRH60909	AT1G20760.1	24529374
AT3G50590.1	KRG94459	AT3G01780.1	24529374
AT3G50590.1	KRH06999	AT3G01780.1	24529374
AT3G50590.1	KRG89827	AT5G24710.1	24529374
AT3G50590.1	KRG96637	AT5G24710.1	24529374
AT3G50590.1	KRG99486	AT5G24710.1	24529374
AT3G50590.1	KRH68370	AT5G24710.1	24529374
AT3G50590.1	KRG90331	AT5G57460.1	24529374
AT3G50590.1	KRH02873	AT5G57460.1	24529374

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH28498	Soybean	cytosol	12.33	93.9
VIT_00s1373g00010.t01	Wine grape	cytosol	8.69	90.38
KRH57890	Soybean	endoplasmic reticulum	83.17	83.17
KRH04644	Soybean	endoplasmic reticulum	82.43	82.43
Solyc02g090570.2.1	Tomato	nucleus	72.63	72.72
Bra036092.1-P	Field mustard	cytosol	68.43	71.34
AT3G50590.1	Thale cress	cytosol	69.54	69.89
GSMUA_Achr10P...	Banana	cytosol	66.46	68.36
Os09t0407950-01	Rice	plasma membrane	40.94	66.87
KXG38043	Sorghum	cytosol	66.52	65.16
TraesCS7D01G009600.1	Wheat	golgi	65.17	64.77
TraesCS7A01G009900.1	Wheat	cytosol	64.12	64.68
TraesCS4A01G485300.1	Wheat	cytosol	65.1	64.63
Zm00001d033368_P007	Maize	cytosol	64.55	63.22
HORVU5Hr1G067090.1	Barley	cytosol	65.1	62.56
Os09t0408000-01	Rice	cytosol, nucleus	17.51	58.44
CDY26515	Canola	cytosol, nucleus, plastid	6.66	42.69

Protein Annotations

Gene3D:2.130.10.10	MapMan:22.1.4.4	UniProt:A0A0R0LLH2	EMBL:ACUP02000649	EnsemblPlantsGene:GLYMA_01G184500	GO:GO:0000045
GO:GO:0000421	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005773	GO:GO:0008150	GO:GO:0008152	GO:GO:0009056
GO:GO:0009987	GO:GO:0016020	GO:GO:0016043	InterPro:IPR001680	InterPro:IPR015943	InterPro:IPR017986
EnsemblPlants:KRH76964	ProteinID:KRH76964	ProteinID:KRH76964.1	PFscan:PS50294	PANTHER:PTHR19878	PANTHER:PTHR19878:SF4
SMART:SM00320	SUPFAM:SSF50978	UniParc:UPI0006EDB98C	InterPro:WD40/YVTN_repeat-like_dom_sf	InterPro:WD40_repeat	InterPro:WD40_repeat_dom
InterPro:WD40_repeat_dom_sf	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr1:+:51972984..51992165

Molecular Weight (calculated)

177140.0 Da

IEP (calculated)

8.446

GRAVY (calculated)

-0.162

Length

1622 amino acids

Sequence
(BLAST)

0001: MEWTTLQHLD LRHVGRGVRP LQPHAATFHP HQALVAVAIG TFIVEFDALT GSKISALDIG APVVRMLYSP TRGHTVIAIL QDSTIRSCDF DFEQTCVLHS
0101: PEKKTEQISS DTEVHLALTP LQSIVFFGFH KRLSVTDFLG YICFCTNEVV GTVEGGRAPT KIKTDLKKPI VNLACHPRLP VLYVAYAEGL IRAYNIHTYA
0201: VHYTLQLDNT IKLNGAGAFA FHPTLEWIFV GDRRGTLLAW DVSTERPSII GLTQVGSQPI TSVSWLTTLC LLVTLSRDGS LQVWKTRVIV NPNTPPMPAS
0301: FFVPAAIESL DIPRILSQQG GEAVYPLPRI KALEFHPKSN LAALVFANVT SGDPLKNKTT YSRERRKQLF SVLQSARGSS ASTLKEKLSA LGSSGVLADH
0401: QLQAQLQEHH LKGHNHLTIL DIGRKAFLYS HFMEGNTKSA PISRLPLITI LDTKHYLKDF PVIQPFHLEL NFFNKENRVL HYPVRAFYVD GPNLMAHNLS
0501: SGSDSIYKKL YNSIPRSVEY QAKYLIYSKK QHLFLVAYEF SGTTNEVVLY RENTDAEISN SKSSTVKGRD AAFIGPNENQ FAILDDDKTG LAVYTLPGGA
0601: SQETKENDKL FEENQPTETT VGSIQGPTPF MFETEVDRIY STPLDSTLMF ASHGNQIGLV KLIQGYRLST SSSKSNGHYI STKSEGKKSI NLKRNEIVLQ
0701: VYWQETLRGH VAGILTTQRV LIVSAAFDIL AGTSTNFDKG LPSFRSLLWV GPALLFSTAT AISILGWDGK VRTILSTSVP YAVLVGALND RLLLASPTEI
0801: NPKQKKGVEI KSCLVGLLEP LLIGFATMQQ SFEQKLDLSE ILYQITSRFD SLRITPRSLD ILARGSPVCG DLAVSLSQLG PHFTQVMRGV YAVKALRFSS
0901: ALSVLKDEFL RSRDYPRCPP TCHLFHRFRQ LGYACIRFGQ FDRAKETFEV TADYKSMLDL FICHLNPSAM RRLAQKLEDE DLDSELRRHC EWILRVRSSG
1001: WTQGIFANFA AESMVPKGPE WGGGDWEIKT PTNAKDIPQW ELAAEVLPYM KTDDGAIPSI ILDHIGVYVG SIKGRGNVVE VREDSLVKAV IPAGNDFKAN
1101: GLEISSVKPI SNQRVDNSQG GPLSLNKQLA SSSTDEQAKA AEEFKKSMYG AAAADSSSDE EGVSKTKKIR VRIRDKPIAS STVDVNKIKE ATSKFKLSGG
1201: LTPTRSRSFT SGSQDLDQIL SLPPAATGVS ARTVSTPGDL FGTDVFTQPE PISQPTTGVA SRGNKVGPIP EDFFQNTISS LQAAASLAPA GTYLSKFAAG
1301: AESGKETRNQ VSASKADVSL QGDVPPQVVQ QPAVPIESGG LPDGGVPPQS SAQASAMPPS QLQEPTSSQP LDLSIFGVPN ASDSGKPPQT GSPPSSVRPG
1401: QVPREAAASV CFKTGLAHLE LNHLSDALSC FDESFLALAK EQSRGSDIKA QATICAQYKI TVTLLQEIGR LQKVHGPSAI SAKDEMARLS RHLGSLPLLA
1501: KHRINCIRTA IKRNMEVQNY AYSKQMLELL LSKAPPSKQE EFRSLIDLCV QRGLTNKSID PLEDPSQFCA FTLSRLSTIG YDVCDLCGSK FSAVTAPGCI
1601: ICGMGSIKRS DALAGPVPSP FG

Best Arabidopsis Sequence Match ( AT3G50590.2 )
(BLAST)

0001: MEWATVQHLD LRHVGRGVSK PLQPHTAAFH PSQAVIAVAV GSHIMEFDAL TGCKIASIDI GSPAVRMLYS PTSSNAVVAI LEDCTIRSCD FETEQTCVLH
0101: SPEKRSEHIS SDTEVHLAVT PLQPVVFFGF PKRMSVTVVG TVEGGRAPTK IKTDLKKPIV NIACHPRLPV LYVAYAEGLI RAYNIHTYAV HYTLQLDNTI
0201: KLIGASSFAF HPTLEWIFVG DRRGTLLAWD VSTERPNMIG ITQVGSQPIT SISWLPMLRV LVTVSKDGSL QVWKTRVIIN PNRPSTQTNF FEPAAMESID
0301: IPKILSQQGG EAVYPLPRIK TLEVHPKLNL AALIFANMVG NENTQNRAAQ TREGRKQLFA VLQSARGSSA SVLKEKLSSM GSSGILAEHQ LQALLQEHKG
0401: QSQLTISDIA RKAFLYSHFM EGHAKTAPIS RLPLITVVDT KDQLKDIPVC QPFHLELNFF NKPNRVLHYP VRSFYIEGLN LMAHNLCSGT DNIYKKLYTS
0501: IPGNVEYHSK HIVYSRKRHL FLVVFEFSGA TNEVVLYWEN TGSQLPNSKG STAKGCDAAF IGPNDDQFAI LDEDKTGLSM YILPKLTTME ENEKNLLSEE
0601: NQKKEADPSG IQGPQQFMFE TEVDRVFSTP IESTLMFACN GTQIGLAKLF QGYRLSASDG HYISTQGEGR KSIKLKKHEI ALQVQWQETP RGYVAGILTT
0701: QRVLMVSADF DILASSSTKY DRGLPSFRSL LWVGPALLFS TTTAVCLLGW DGKVRTILSI STPYAALVGA LNDRLLLAHP TDISPKQKKG IEIKSCLVGL
0801: LEPLLIGFST MQQTFEQKVD LSEILYQITT RFDSLRITPR SLDILARSAP VCGDLAVSLA QAGPQFNQVL RCAYAIKALR FSTALSVLKD EFLRSRDYPK
0901: CPPTSLLFQR FRQLGYACIK YGQFDSAKET FEVIGDYESM LDLFICHLNP SAMRRLAQKL EEESGDPELR RYCERILRVR STGWTQGIFA NFAAESMVPK
1001: GPEWGGGNWE IKTPTDMKSI PKWELAGEVM PYMKNEDGTI PSIVADHIGV YLGCVKGRVN VVEIKEDSLV SKPGGLSLLG KPVSDKPLAL PAGESSSMMG
1101: LESLGKQNVA DEQAKAAEEF KKTMYGATGD GSSSDEEGVT KPKKLQIRIR EKPTSTTVDV NKLKEAAKTF KLGDGLGLTM SRTKSINAGS QDLGQMLSQP
1201: SSSTVATTTA PSSASAPVDP FAMSSWTQQP QPVSQPAPPG VAAPIPEDFF QNTIPSVEVA KTLPPPGTYL SKMDQAARAA IAAQGGPNQA NNTPLPDIGL
1301: PDGGVPQQYP QQTSQQPGAP FQTVGLPDGG VRQQYPGQNQ VPSQVPVSTQ PLDLSVLGVP NTGDSGKPPG QPQSPPASVR PGQVPRGAAA PVCFKTGLAH
1401: LEQNQLPDAL SCFDEAFLAL AKDQSRGADI KAQATICAQY KIAVTLLREI LRLQRVQGAS ALSAKDEMAR LSRHLASLPL LAKHRINCIR TAIKRNMEVQ
1501: NYGYSKQMLE LLLSKAPASK QEELRGLVDL CVQRGTSNKS IDPLEDPSQL CSATLSRLST IGYDVCDLCG AKFAALSSPG CIICGMGSIK RSDALAGPAP
1601: VSTPFG

Arabidopsis Description

Transducin/WD40 repeat-like superfamily protein [Source:UniProtKB/TrEMBL;Acc:F4J0P2]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.