Factsheet for Os05t0455500-02

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 4
plastid 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 18260611
plasma membrane: 27800704
plastid: 27992503
plasma membrane: 28056797

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0611100-01
Os01t0848200-02
Os05t0455500-02

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G39800.1	Os01t0848200-02	AT2G39800.1	21798944
AT2G39800.1	Os05t0455500-02	AT2G39800.1	21798944
AT2G39800.1	Os01t0611100-01	AT5G20010.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KXG22139	Sorghum	cytosol	88.41	88.41
HORVU1Hr1G072780.1	Barley	cytosol	88.41	88.41
TraesCS1A01G281400.1	Wheat	cytosol	88.27	88.27
TraesCS1B01G290600.2	Wheat	mitochondrion	87.99	87.99
TraesCS1D01G280700.2	Wheat	golgi	87.71	87.71
Zm00001d038358_P002	Maize	cytosol	84.78	83.26
Zm00001d010056_P002	Maize	cytosol	88.27	82.29
GSMUA_Achr10P...	Banana	cytosol	13.69	81.67
VIT_13s0019g02360.t01	Wine grape	cytosol	78.21	78.1
KRH73083	Soybean	endoplasmic reticulum	77.65	77.98
Os01t0848200-02	Rice	plasma membrane	79.61	77.55
KRH15055	Soybean	nucleus	77.09	77.42
KRG97830	Soybean	endoplasmic reticulum	75.98	76.08
Solyc08g043170.2.1	Tomato	plastid	76.12	75.69
Solyc06g019170.2.1	Tomato	nucleus, plastid, unclear	75.42	75.31
PGSC0003DMT400068829	Potato	cytosol	75.14	75.03
Bra005012.1-P	Field mustard	cytosol	75.0	74.9
AT2G39800.1	Thale cress	cytosol	75.0	74.9
CDY20482	Canola	cytosol	74.86	74.76
CDX74830	Canola	cytosol	74.86	74.76
CDX93233	Canola	cytosol	74.58	74.48
CDY07034	Canola	cytosol	74.58	74.48
CDY07635	Canola	cytosol	74.3	74.41
Bra000150.1-P	Field mustard	cytosol	74.3	74.41
CDY61781	Canola	cytosol	74.16	74.27
CDX76082	Canola	cytosol	74.16	74.16
Bra017051.1-P	Field mustard	cytosol	74.16	74.06
PGSC0003DMT400068827	Potato	cytosol	65.22	73.89
Bra014732.1-P	Field mustard	cytosol	74.02	73.71
CDY41203	Canola	cytosol	74.44	73.31
CDY40241	Canola	cytosol	74.3	73.18
Bra007179.1-P	Field mustard	cytosol	74.3	73.18
AT3G55610.1	Thale cress	cytosol	74.16	73.14
CDY65768	Canola	cytosol	73.74	72.73
GSMUA_Achr10P...	Banana	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	26.4	63.42

Protein Annotations

KEGG:00330+1.2.1.41	KEGG:00330+2.7.2.11	KEGG:00330+2.7.2.11+1.2.1.41	KEGG:00332+1.2.1.41	KEGG:00332+2.7.2.11	KEGG:00332+2.7.2.11+1.2.1.41
Gene3D:3.40.1160.10	Gene3D:3.40.309.10	Gene3D:3.40.605.10	MapMan:4.1.1.1.4.1.1	EntrezGene:4338979	ProteinID:AAU90213.1
EMBL:AK102633	EMBL:AY574031	InterPro:AceGlu_kinase-like_sf	InterPro:Ald_DH/histidinol_DH	InterPro:Ald_DH_C	InterPro:Ald_DH_N
InterPro:Aldehyde_DH_dom	InterPro:Asp/Glu/Uridylate_kinase	ProteinID:BAF17637.1	ProteinID:BAS94357.1	EMBL:D49714	InterPro:G-glutamylP_reductase_CS
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004349	GO:GO:0004350	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006561
GO:GO:0008150	GO:GO:0008152	GO:GO:0008652	GO:GO:0009058	GO:GO:0009987	GO:GO:0016020
GO:GO:0016021	GO:GO:0016301	GO:GO:0016310	GO:GO:0016491	GO:GO:0016620	GO:GO:0016740
GO:GO:0055114	GO:GO:0055129	InterPro:GPR_dom	InterPro:Glu/AcGlu_kinase	InterPro:Glu_5kinase/COase_Synthase	InterPro:Glutamate_5-kinase_CS
InterPro:IPR016162	InterPro:IPR016163	InterPro:IPR036393	HAMAP:MF_00412	HAMAP:MF_00456	UniProt:O04226
EnsemblPlantsGene:Os05g0455500	EnsemblPlants:Os05t0455500-02	InterPro:P5_carboxy_syn	PFAM:PF00171	PFAM:PF00696	PIRSF:PIRSF036429
PRINTS:PR00474	ScanProsite:PS00902	ScanProsite:PS01223	PANTHER:PTHR11063	PANTHER:PTHR11063:SF13	SUPFAM:SSF53633
SUPFAM:SSF53720	TIGRFAMs:TIGR00407	TIGRFAMs:TIGR01027	TIGRFAMs:TIGR01092	UniParc:UPI000044479A	RefSeq:XP_015640176.1
SEG:seg	:	:	:	:	:

Description

P5C SYNTHETASESimilar to Delta-1-pyrroline-5-carboxylate synthetase. (Os05t0455500-01);Similar to Delta 1-pyrroline-5-carboxylate synthetase (P5CS). (Os05t0455500-02)

Coordinates

chr5:-:22374029..22380819

Molecular Weight (calculated)

77749.8 Da

IEP (calculated)

6.829

GRAVY (calculated)

-0.060

Length

716 amino acids

Sequence
(BLAST)

001: MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS LGYEVILVTS GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ
101: SGLMALYDML FNQLDVSSSQ LLVTDSDFEN PKFREQLTET VESLLDLKVI PIFNENDAIS TRKAPYEDSS GIFWDNDSLA GLLALELKAD LLILLSDVDG
201: LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT AKVKAAVLAS NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV
301: AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG YEKPLVARLT IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK
401: TSCPLGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE AIRSNTILHK VITDAIPRNV GEKLIGLVTT RDEIADLLKL DDVIDLVIPR GSNKLVSQIK
501: ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP AACNAMETLL VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM
601: ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH NASTRFSDGA RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV
701: NGDKDVVYTH KSLPLQ

Best Arabidopsis Sequence Match ( AT3G55610.1 )
(BLAST)

001: MTEIDRSRAF AKDVKRIVVK VGTAVVTGKG GRLALGRLGA ICEQLAELNS DGFEVILVSS GAVGLGRQRL RYRQLVNSSF ADLQKPQMEL DGKACAGVGQ
101: SSLMAYYETM FDQLDVTVAQ MLVTDSSFRD KDFRKQLSET VKAMLRMRVI PVFNENDAIS TRRAPYKDST GIFWDNDSLA ALLSLELKAD LLILLSDVEG
201: LYTGPPSDST SKLIHTFIKE KHQDEITFGE KSKLGRGGMT AKVKAAVNAA YGGVPVIITS GYAAENISKV LRGLRVGTLF HQDAHLWAPV VDTTSRDMAV
301: AARESSRKLQ ALSSEDRKQI LHDIANALEV NEKTIKAEND LDVAAAQEAG YEESLVARLV MKPGKISSLA ASVRQLAEME DPIGRVLKKT QVADDLILEK
401: TSSPIGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE ARRSNAILHK VITDAIPETV GGKLIGLVTS REEIPDLLKL DDVIDLVIPR GSNKLVSQIK
501: NSTKIPVLGH ADGICHVYVD KSGKLDMAKR IVSDAKLDYP AACNAMETLL VHKDLEQNGF LDDLIYVLQT KGVTLYGGPR ASAKLNIPET KSFHHEYSSK
601: ACTVEIVEDV YGAIDHIHQH GSAHTDCIVT EDSEVAEIFL RQVDSAAVFH NASTRFSDGF RFGLGAEVGI STSRIHARGP VGVEGLLTTR WIMRGKGQVV
701: DGDNGIVYTH KDLPVLQRTE AVENGI

Arabidopsis Description

P5CSBDelta-1-pyrroline-5-carboxylate synthase [Source:UniProtKB/TrEMBL;Acc:A0A178VF78]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.