CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 3
cytosol 2
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG95394
KRH43388
KRH59529
KRH67075

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G04950.1	KRH43388	AT5G09830.1	21798944
AT4G04950.1	KRH59529	AT5G09830.1	21798944
AT4G04950.1	KRG95394	AT5G22355.1	21798944
AT4G04950.1	KRH67075	AT5G22355.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
VIT_10s0597g00030.t01	Wine grape	cytosol	79.8	79.47
CDY16635	Canola	cytosol	44.69	71.34
GSMUA_Achr2P10580_001	Banana	cytosol	69.8	70.95
GSMUA_Achr8P11270_001	Banana	cytosol	63.27	70.61
Bra029532.1-P	Field mustard	cytosol	48.57	70.21
AT4G04950.1	Thale cress	cytosol	69.59	69.88
CDY67888	Canola	cytosol	50.0	69.6
Solyc02g078360.2.1	Tomato	cytosol	42.04	69.59
Bra000810.1-P	Field mustard	cytosol	67.55	69.54
CDX90882	Canola	cytosol	67.14	68.97
TraesCS1B01G188000.1	Wheat	cytosol	67.14	67.28
Os10t0500700-01	Rice	cytosol	66.73	66.6
Zm00001d006437_P001	Maize	cytosol	42.45	66.45
TraesCS1D01G166900.1	Wheat	cytosol	65.92	66.05
Zm00001d013927_P001	Maize	extracellular	66.94	65.73
TraesCS1A01G168900.1	Wheat	cytosol	65.51	65.64
CDY21322	Canola	cytosol	67.96	65.55
Zm00001d032546_P001	Maize	extracellular	66.53	65.33
KXG38234	Sorghum	cytosol	66.33	65.13
AT4G32580.1	Thale cress	cytosol	21.02	64.38
Zm00001d016478_P001	Maize	cytosol	36.12	57.47
Zm00001d011577_P001	Maize	cytosol	47.76	47.85
Zm00001d006222_P001	Maize	cytosol	44.69	43.89
KRH09770	Soybean	cytosol	4.29	39.62
KRH40338	Soybean	plastid	9.59	29.56
KRH00894	Soybean	plastid	10.61	29.38
KRH23549	Soybean	mitochondrion	9.59	29.01
KRH47491	Soybean	cytosol, plastid	9.18	27.95
KRH44417	Soybean	cytosol, plastid	9.18	27.95
KRH40339	Soybean	cytosol	0.82	23.53
KRH65709	Soybean	nucleus, plastid	13.47	22.37
KRH75953	Soybean	nucleus	13.06	20.0

Protein Annotations

EntrezGene:100784787	Gene3D:3.40.30.10	MapMan:35.1	EMBL:ACUP02008265	EnsemblPlantsGene:GLYMA_13G180400	GO:GO:0003674
GO:GO:0003824	GO:GO:0005575	GO:GO:0005623	GO:GO:0006091	GO:GO:0008150	GO:GO:0008152
GO:GO:0009055	GO:GO:0009987	GO:GO:0015035	GO:GO:0019725	GO:GO:0022900	GO:GO:0045454
GO:GO:0055114	InterPro:GRX_PICOT-like	InterPro:Glutaredoxin	UniProt:I1M0B5	InterPro:IPR002109	InterPro:IPR013766
EnsemblPlants:KRH20463	ProteinID:KRH20463	ProteinID:KRH20463.1	InterPro:Monothiol_GRX-rel	PFAM:PF00085	PFAM:PF00462
PFscan:PS51352	PFscan:PS51354	SUPFAM:SSF52833	TIGRFAMs:TIGR00365	InterPro:Thioredoxin-like_sf	InterPro:Thioredoxin_domain
UniParc:UPI0001D7CCBB	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr13:-:29380779..29383908

Molecular Weight (calculated)

53810.9 Da

IEP (calculated)

4.985

GRAVY (calculated)

-0.372

Length

490 amino acids

Sequence
(BLAST)

001: MGGSVRDVKS KGEVDEVVAS GSPVILHFWA SWCEASKHMD QLFSHLSTDF PNARFLRVEA EEQPEISEAY SVSAVPFFAF CKDGKTFDTL EGADPSSLAN
101: KVAKVAGSIN PGESASPASL GMAAGASVLE TVKELAKDND SSKEKNQVQP GLSGPLKKRI QQLVDSNPVM LFMKGTPEEP KCGFSRKVVV VLNEERVKFG
201: SFDVLSDSEV REGLKKFSNW PTFPQLYCKG ELLGGCDIAI AMHESGELKE VFKDHGIDTT NEAKEKESGN GKGGISKSTD LSTTLSSRLE SLVNSSAVML
301: FMKGKPDEPK CGFSRKVVEI LQQENVPFES FDILTDEEVR QGLKVYSNWS SYPQLYIKGE LIGGSDIVLE MQKSGELRKN LHEKGILPAE TIQDRLKNLI
401: ASSPVMLFMK GTPDAPRCGF SSRVADALRQ EGLNFGSFDI LTDEEVRQGL KVYSNWPTYP QLYYKSELIG GHDIVMELRN NGELKSTLSE

Best Arabidopsis Sequence Match ( AT4G04950.1 )
(BLAST)

001: MSGTVKDIVS KAELDNLRQS GAPVVLHFWA SWCDASKQMD QVFSHLATDF PRAHFFRVEA EEHPEISEAY SVAAVPYFVF FKDGKTVDTL EGADPSSLAN
101: KVGKVAGSST SAEPAAPASL GLAAGPTILE TVKENAKASL QDRAQPVSTA DALKSRLEKL TNSHPVMLFM KGIPEEPRCG FSRKVVDILK EVNVDFGSFD
201: ILSDNEVREG LKKFSNWPTF PQLYCNGELL GGADIAIAMH ESGELKDAFK DLGITTVGSK ESQDEAGKGG GVSSGNTGLS ETLRARLEGL VNSKPVMLFM
301: KGRPEEPKCG FSGKVVEILN QEKIEFGSFD ILLDDEVRQG LKVYSNWSSY PQLYVKGELM GGSDIVLEMQ KSGELKKVLT EKGITGEQSL EDRLKALINS
401: SEVMLFMKGS PDEPKCGFSS KVVKALRGEN VSFGSFDILT DEEVRQGIKN FSNWPTFPQL YYKGELIGGC DIIMELSESG DLKATLSE

Arabidopsis Description

GRXS17Monothiol glutaredoxin-S17 [Source:UniProtKB/Swiss-Prot;Acc:Q9ZPH2]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.