CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 2
plasma membrane 1
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG99414
KRH45615
KRH69617
KRH74545

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G23460.1	KRG99414	AT1G60780.1	23543752
AT4G23460.1	KRH45615	AT1G60780.1	23543752
AT4G23460.1	KRH69617	AT1G60780.1	23543752
AT4G23460.1	KRH74545	AT1G60780.1	23543752

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH27813	Soybean	endoplasmic reticulum, nucleus	97.53	96.77
KRH29071	Soybean	cytosol, endoplasmic reticulum	85.3	92.12
KRH24097	Soybean	endoplasmic reticulum	92.26	91.54
PGSC0003DMT400031784	Potato	cytosol	87.21	87.01
Solyc08g081320.2.1	Tomato	cytosol, extracellular, nucleus, plastid	87.43	86.94
VIT_02s0025g00100.t01	Wine grape	cytosol	89.45	86.63
AT4G23460.1	Thale cress	cytosol	86.53	86.34
Bra019302.1-P	Field mustard	cytosol	85.07	86.14
Os03t0355600-01	Rice	plasma membrane	86.2	86.0
GSMUA_AchrUn_...	Banana	cytosol	86.64	85.59
Bra035228.1-P	Field mustard	cytosol	86.31	85.54
CDY13257	Canola	cytosol	86.31	85.54
GSMUA_Achr1P24200_001	Banana	cytosol	86.42	85.37
EER94839	Sorghum	cytosol	85.75	85.17
Zm00001d047464_P024	Maize	cytosol, mitochondrion, plastid	85.75	85.08
CDX94190	Canola	cytosol	85.63	84.78
TraesCS4B01G165000.1	Wheat	cytosol, golgi	85.41	84.74
TraesCS4D01G166700.1	Wheat	cytosol	85.41	84.74
Zm00001d029196_P002	Maize	cytosol	80.92	84.53
HORVU4Hr1G045980.10	Barley	cytosol	85.07	84.32
AT4G11380.2	Thale cress	cytosol	86.64	84.28
TraesCS4A01G166200.2	Wheat	cytosol	85.19	84.24
Zm00001d030865_P001	Maize	cytosol	6.73	83.33
Zm00001d032260_P001	Maize	plastid	12.12	68.35
CDX86788	Canola	cytosol	86.42	66.67
Zm00001d018154_P001	Maize	extracellular	14.03	35.11
KRH52665	Soybean	plastid	27.95	29.47
KRH62012	Soybean	plastid	27.72	29.23
KRH15094	Soybean	cytosol, peroxisome, plastid	24.02	18.94
KRH73041	Soybean	cytosol, peroxisome, plastid	23.68	18.67

Protein Annotations

Gene3D:1.25.10.10	EntrezGene:100777522	Gene3D:2.60.40.1150	MapMan:22.1.2.2	MapMan:22.1.3.2	Gene3D:3.30.310.10
EMBL:ACUP02000681	InterPro:AP_beta	InterPro:AP_complex_bsu_1_2_4	InterPro:ARM-like	InterPro:ARM-type_fold	InterPro:B-adaptin_app_sub_C
InterPro:Clathrin/coatomer_adapt-like_N	InterPro:Clathrin_a/b/g-adaptin_app_Ig	InterPro:Clathrin_app_Ig-like_sf	InterPro:Clathrin_b-adaptin_app_Ig-like	InterPro:Coatomer/calthrin_app_sub_C	EnsemblPlantsGene:GLYMA_01G227000
GO:GO:0003674	GO:GO:0005215	GO:GO:0005488	GO:GO:0005515	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006810	GO:GO:0006886	GO:GO:0008150	GO:GO:0008565
GO:GO:0015031	GO:GO:0016020	GO:GO:0016192	GO:GO:0030117	GO:GO:0030131	GO:GO:0030276
UniProt:I1JAF7	InterPro:IPR011989	InterPro:IPR012295	InterPro:IPR013037	EnsemblPlants:KRH77672	ProteinID:KRH77672
ProteinID:KRH77672.1	PFAM:PF01602	PFAM:PF02883	PFAM:PF09066	PIRSF:PIRSF002291	PANTHER:PTHR11134
PANTHER:PTHR11134:SF3	SMART:SM00809	SMART:SM01020	SUPFAM:SSF48371	SUPFAM:SSF49348	SUPFAM:SSF55711
InterPro:TBP_dom_sf	UniParc:UPI00023377FF	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr1:+:55544798..55552158

Molecular Weight (calculated)

99088.6 Da

IEP (calculated)

4.671

GRAVY (calculated)

-0.065

Length

891 amino acids

Sequence
(BLAST)

001: MSRNDSKYFS TTKKGEIPEL KEELNSQYKD KRKDAVKKVI AAMTVGKDVS SLFTDVVNCM QTENLELKKL VYLYLINYAK SQPDLAILAV NTFVKDSQDP
101: NPLIRALAVR TMGCIRVDKI TEYLCDPLQR CLKDDDPYVR KTAAICVAKL YDINAELVED RGFLESLKDL ISDNNPMVVA NAVAALAEVQ ENSSRPIFEI
201: TSHTLSKLLT ALNECTEWGQ VFILDALSRY KAADAREAEN IVERVTPRLQ HANCAVVLSA VKMILLQMEL ITSTDVVRNL CKKMAPPLVT LLSAEPEIQY
301: VALRNINLIV QRRPTILAHE IKVFFCKYND PIYVKMEKLE IMIKLASDRN IDQVLLEFKE YATEVDVDFV RKAVRAIGRC AIKLERAAER CISVLLELIK
401: IKVNYVVQEA IIVIKDIFRR YPNTYESIIA TLCESLDTLD EPEAKASMIW IIGEYAERID NADELLESFL ESFPEEPAQV QLQLLTATVK LFLKKPTEGP
501: QQMIQVVLNN ATVETDNPDL RDRAYIYWRL LSTDPEAAKD VVLAEKPVIT DDSNQLEPSL LDELLANIAT LSSVYHKPPD AFVTRVHSAQ RTEDEDFAEG
601: SETGFSESPA NPANGPASPP TSATGAPATP PSVAPVPDLL GDLMGMDNSI VPVDQPVTPT GPPLPILLPA STGQGLQISA QLTRQDGQIF YSLLFENNSQ
701: VSLDGFMIQF NKNTFGLAAA GPLQVPQLQP GMSARTLLPM VMFQNMSQGP PSSVLQVAVK NNQQPVWYFS DKISLLVFFT EDGRMERSSF LETWRSLPDS
801: NEVSKDFPAI VIGSADATVE RLAASNMFFI AKRKNANQDV FYFSAKLPRG IPFLIELTTL TGNPGVKCAI KTPSPEMSAL FFEAIETLLM G

Best Arabidopsis Sequence Match ( AT4G23460.1 )
(BLAST)

001: MSGHDSKYFS TTKKGEIPEL KEELNSQYKD KRKDAVKKVI AAMTVGKDVS SLFTDVVNCM QTENLELKKL VYLYLINYAK SQPDLAILAV NTFVKDSQDP
101: NPLIRALAVR TMGCIRVDKI TEYLCDPLQK CLKDDDPYVR KTAAICVAKL FDINAELVED RGFLEALKDL ISDNNPMVVA NAVAALAEIQ ENSTSPIFEI
201: NSTILTKLLT ALNECTEWGQ VFILDALSRY KASDPREAEN IVERVTPRLQ HANCAVVLSA VKMILQQMEL ITSTDVIRNL CKKMAPPLVT LLSAEPEIQY
301: VALRNINLIV QKRPTILAHE IKVFFCKYND PIYVKMEKLE IMIKLASDRN IDQVLLEFKE YATEVDVDFV RKAVRAIGRC AIKLERAAER CISVLLELIK
401: IKVNYVVQEA IIVIKDIFRR YPNTYESIIA TLCESLDTLD EPEAKASMIW IIGEYAERID NADELLESFL ENFPEEPAQV QLQLLTATVK LFLKKPTEGP
501: QQMIQVVLNN ATVETDNPDL RDRAYIYWRL LSTDPEAAKD VVLAEKPVIT DDSNQLDPSL LDELLANIST LSSVYHKPPE AFVTRLKTTV QKTEDEDYVE
601: GSETGYPEAS GNPVDGAASP SATTGYVTKL AAAPAPVPDL LGDLMGSDNA AIVPVDEPTT PSGRPLPVVL PASKGQGLQI SAQLTRQDGQ VFYSMLLENN
701: SQSLLDGFMI QFNKNSFGLA AVGSLQVPPL QPGASARTMM PMVLSQNMST GSTSSVLQVA VKNNQQPVWY FEDKIVLNAL FSEDGRMERG TFLETWKSLP
801: DSNEVQKEFP GITITSVEST LDLLAASNMF FIAKRKNGNQ DVLYLSAKVP RGIPFLIELT AIVGQPGLKC AVKTPTPEIA PLFFEAVEIL FKA

Arabidopsis Description

BETAC-ADBeta-adaptin-like protein C [Source:UniProtKB/Swiss-Prot;Acc:O81742]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.