CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 2
cytosol 4
plastid 1
mitochondrion 2
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, nucleus, plastid, secretory
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	nucleus, plastid
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

endoplasmic reticulum: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG99414
KRH45615
KRH69617
KRH74545

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G60780.1	KRG99414	AT1G60780.1	23543752
AT1G60780.1	KRH45615	AT1G60780.1	23543752
AT1G60780.1	KRH69617	AT1G60780.1	23543752
AT1G60780.1	KRH74545	AT1G60780.1	23543752

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH69617	Soybean	cytosol	99.07	99.07
KRG99414	Soybean	cytosol	96.03	96.03
KRH45615	Soybean	cytosol	95.79	95.79
VIT_00s0225g00220.t01	Wine grape	cytosol	91.12	91.12
Solyc05g013340.2.1	Tomato	plastid	90.19	90.61
AT1G60780.1	Thale cress	cytosol	90.42	90.42
Bra031481.1-P	Field mustard	cytosol	90.19	90.19
CDY19316	Canola	cytosol	90.19	90.19
PGSC0003DMT400002906	Potato	mitochondrion	46.26	90.0
CDY63681	Canola	cytosol	89.95	89.95
CDX97909	Canola	cytosol	89.95	89.95
GSMUA_Achr7P17600_001	Banana	cytosol	89.95	89.95
Bra019904.1-P	Field mustard	cytosol	89.72	89.72
CDY46183	Canola	cytosol	89.49	89.49
CDY54239	Canola	cytosol	89.49	89.49
CDY61312	Canola	cytosol	89.49	89.49
Bra031752.1-P	Field mustard	cytosol	88.55	89.39
Os01t0703600-01	Rice	plasma membrane	88.55	88.34
Zm00001d043726_P001	Maize	cytosol	88.55	88.34
EES01338	Sorghum	cytosol	88.55	88.34
GSMUA_Achr2P06510_001	Banana	cytosol, endoplasmic reticulum	88.32	88.32
GSMUA_Achr1P25430_001	Banana	cytosol, extracellular	88.32	88.32
Zm00001d011676_P001	Maize	extracellular, plasma membrane	88.32	88.11
CDY63680	Canola	cytosol	89.02	87.99
HORVU3Hr1G066020.1	Barley	cytosol	87.85	87.65
TraesCS1B01G383200.1	Wheat	cytosol	87.62	87.41
TraesCS1A01G365600.1	Wheat	golgi	87.62	87.41
HORVU1Hr1G079050.3	Barley	cytosol	87.62	87.41
Os05t0543100-01	Rice	plasma membrane	87.62	87.21
TraesCS3D01G255100.2	Wheat	cytosol	87.15	86.95
TraesCS3A01G254200.2	Wheat	cytosol	86.92	86.71
TraesCS3B01G286200.2	Wheat	cytosol	86.68	86.48
AT1G10730.1	Thale cress	cytosol	85.51	85.51
Solyc04g026380.2.1	Tomato	cytosol	46.5	84.68
Solyc04g026360.2.1	Tomato	nucleus	43.69	82.38
TraesCS1D01G371000.1	Wheat	cytosol	86.45	81.14
Zm00001d052338_P001	Maize	cytosol	10.98	79.66
Zm00001d036168_P001	Maize	mitochondrion	36.22	63.79
GSMUA_Achr8P31910_001	Banana	cytosol	16.82	44.72
KRH58201	Soybean	mitochondrion	41.82	40.87
KRH04335	Soybean	endoplasmic reticulum	41.82	40.87
KRH77207	Soybean	cytosol, extracellular	41.36	40.41
KRH28283	Soybean	cytosol, golgi	41.12	40.18
KRH16840	Soybean	cytosol	32.71	30.97
KRH72454	Soybean	cytosol	28.5	30.27
KRH61131	Soybean	cytosol, nucleus	30.84	29.8
KRH51806	Soybean	cytosol	25.93	28.83
KRH33789	Soybean	cytosol	24.3	25.06
KRG96704	Soybean	cytosol	24.3	25.06
KRH68423	Soybean	cytosol	23.83	23.34

Protein Annotations

EntrezGene:100808175	Gene3D:2.60.40.1170	MapMan:22.1.2.3	Gene3D:3.30.450.60	EMBL:ACUP02000046	InterPro:AP2_Mu_C_sf
InterPro:AP_mu_sigma_su	InterPro:Clathrin_mu	InterPro:Clathrin_mu_CS	EnsemblPlantsGene:GLYMA_01G027300	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006810	GO:GO:0006886	GO:GO:0008150	GO:GO:0015031
GO:GO:0016020	GO:GO:0016192	GO:GO:0030131	UniProt:I1J553	InterPro:IPR028565	EnsemblPlants:KRH74545
ProteinID:KRH74545	ProteinID:KRH74545.1	InterPro:Longin-like_dom_sf	InterPro:MHD	PFAM:PF00928	PFAM:PF01217
PIRSF:PIRSF005992	PRINTS:PR00314	ScanProsite:PS00990	ScanProsite:PS00991	PFscan:PS51072	PANTHER:PTHR10529
PANTHER:PTHR10529:SF338	SUPFAM:SSF49447	SUPFAM:SSF64356	UniParc:UPI0001CAA4DC	:	:

Description

hypothetical protein

Coordinates

chr1:+:2852073..2856865

Molecular Weight (calculated)

49137.2 Da

IEP (calculated)

6.291

GRAVY (calculated)

-0.214

Length

428 amino acids

Sequence
(BLAST)

001: MAGAASALFL LDIKGRVLIW RDYRGDVSAL DAERFFTKLI EKQADPQAQD PVVHDNGVTY MFIQHSNVYL MIATRQNCNA ASLLFFLHRI VDVFKHYFEE
101: LEEESLRDNF VVVYELLDEI MDFGYPQYTE AQILSEFIKT DAYRMEVTQR PPMAVTNAVS WRSEGINYKK NEVFLDVVES VNILVNSNGQ IIRSDVVGAL
201: KMRTYLSGMP ECKLGLNDRV LLEAQGRTTK GKAIDLEDIK FHQCVRLARF ENDRTISFIP PDGSFDLMTY RLSTQVKPLI WVEASVEKHS KSRIEIMVKA
301: RSQFKERSTA TNVEIELPVP VDATNPNVRT SMGSASYAPE KDALIWKIRS FPGGKEYMLR AEFRLPSITD EEATPERKAP IRVKFEIPYF TVSGIQVRYL
401: KVIEKSGYQA LPWVRYITMA GEYELRLI

Best Arabidopsis Sequence Match ( AT1G60780.1 )
(BLAST)

001: MAGAASALFL LDIKGRVLVW RDYRGDVSAA QAERFFTKLI EKEGDSQSND PVAYDNGVTY MFVQHSNVYL MIASRQNCNA ASLLFFLHRV VDVFKHYFEE
101: LEEESLRDNF VVVYELLDEM MDFGYPQYTE ARILSEFIKT DAYRMEVTQR PPMAVTNAVS WRSEGIQYKK NEVFLDVIEN VNILVNSNGQ IVRSDVVGAL
201: KMRTYLTGMP ECKLGLNDRV LLEAQGRATK GKAIDLEDIK FHQCVRLARF ENDRTISFIP PDGAFDLMTY RLSTQVKPLI WVEAQIESHS RSRVEMLIKA
301: RSQFKERSTA TNVEIELPVP TDASNPTVRT SLGSASYAPE KDALVWKIKS FPGNKEYMLR AEFHLPSITA EEATPERKAP IRVKFEIPYF TVSGIQVRYL
401: KIIEKSGYQA LPWVRYITMA GEYELRLV

Arabidopsis Description

AP1M2AP-1 complex subunit mu-2 [Source:UniProtKB/Swiss-Prot;Acc:O22715]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.