Factsheet for Os05t0543100-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

extracellular 2
endoplasmic reticulum 2
vacuole 2
plasma membrane 2
golgi 2
cytosol 3
plastid 2
nucleus 1
mitochondrion 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, mitochondrion, nucleus, plastid, secretory
BaCelLo:	secretory
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	nucleus, plastid
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	plastid
YLoc:	cytosol

plasma membrane: 28056797

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0703600-01
Os05t0543100-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G60780.1	Os01t0703600-01	AT1G60780.1	23543752
AT1G60780.1	Os05t0543100-01	AT1G60780.1	23543752

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS1B01G383200.1	Wheat	cytosol	93.49	93.71
TraesCS1A01G365600.1	Wheat	golgi	93.49	93.71
Os01t0703600-01	Rice	plasma membrane	93.49	93.71
HORVU1Hr1G079050.3	Barley	cytosol	93.49	93.71
GSMUA_Achr7P17600_001	Banana	cytosol	90.93	91.36
VIT_00s0225g00220.t01	Wine grape	cytosol	90.47	90.89
GSMUA_Achr2P06510_001	Banana	cytosol, endoplasmic reticulum	90.0	90.42
GSMUA_Achr1P25430_001	Banana	cytosol, extracellular	88.6	89.02
Solyc05g013340.2.1	Tomato	plastid	87.21	88.03
KRH74545	Soybean	endoplasmic reticulum	87.21	87.62
KRH69617	Soybean	cytosol	87.21	87.62
TraesCS1D01G371000.1	Wheat	cytosol	92.33	87.06
KRH45615	Soybean	cytosol	86.51	86.92
KRG99414	Soybean	cytosol	86.51	86.92
CDX97909	Canola	cytosol	85.81	86.22
CDY19316	Canola	cytosol	85.81	86.22
AT1G60780.1	Thale cress	cytosol	85.58	85.98
Bra019904.1-P	Field mustard	cytosol	85.58	85.98
PGSC0003DMT400002906	Potato	mitochondrion	43.95	85.91
Bra031752.1-P	Field mustard	cytosol	84.65	85.85
CDY54239	Canola	cytosol	85.35	85.75
CDY61312	Canola	cytosol	85.35	85.75
Bra031481.1-P	Field mustard	cytosol	85.12	85.51
CDY63681	Canola	cytosol	84.88	85.28
CDY46183	Canola	cytosol	84.42	84.81
PGSC0003DMT400069426	Potato	cytosol	26.05	84.21
CDY63680	Canola	cytosol	83.95	83.37
Solyc06g071650.1.1	Tomato	cytosol, plastid	79.53	82.21
Solyc04g026360.2.1	Tomato	nucleus	43.02	81.5
Solyc04g026380.2.1	Tomato	cytosol	44.42	81.28
AT1G10730.1	Thale cress	cytosol	80.7	81.07
Os02t0690700-01	Rice	plasma membrane	39.3	38.58
Os07t0620300-01	Rice	plasma membrane	33.49	31.93
Os05t0383100-01	Rice	cytosol	24.19	24.94

Protein Annotations

Gene3D:2.60.40.1170	MapMan:22.1.2.3	Gene3D:3.30.450.60	EntrezGene:4339486	ProteinID:AAU43995.1	EMBL:AK065138
InterPro:AP2_Mu_C_sf	InterPro:AP_mu_sigma_su	ProteinID:BAF18115.1	ProteinID:BAS95147.1	InterPro:Clathrin_mu	InterPro:Clathrin_mu_CS
ProteinID:EEE64556.1	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006810
GO:GO:0006886	GO:GO:0008150	GO:GO:0015031	GO:GO:0016020	GO:GO:0016192	GO:GO:0030131
InterPro:IPR028565	InterPro:Longin-like_dom_sf	InterPro:MHD	EnsemblPlantsGene:Os05g0543100	EnsemblPlants:Os05t0543100-01	PFAM:PF00928
PFAM:PF01217	PIRSF:PIRSF005992	PRINTS:PR00314	ScanProsite:PS00990	ScanProsite:PS00991	PFscan:PS51072
PANTHER:PTHR10529	PANTHER:PTHR10529:SF338	UniProt:Q65XN2	SUPFAM:SSF49447	SUPFAM:SSF64356	UniParc:UPI000043F3FC
RefSeq:XP_015637934.1	:	:	:	:	:

Description

Similar to Clathrin coat assembly protein AP47 (Clathrin coat associated protein AP47) (Golgi adaptor AP-1 47 kDa protein) (HA1 47 kDa subunit) (Clathrin assembly protein assembly protein complex 1 medium chain) (Uncoordinated protein 101). (Os05t0543100-01)

Coordinates

chr5:-:26952587..26955912

Molecular Weight (calculated)

49249.4 Da

IEP (calculated)

6.043

GRAVY (calculated)

-0.197

Length

430 amino acids

Sequence
(BLAST)

001: MAAGAVSALF LLDIKGRVLV WRDYRGDVSA LQAERFFTKL LDKESDAEVL SPVVHDDAGV SYMFIQHNNV FLLTASRQNC NAASILLFLH RVVDVFKHYF
101: EELEEESLRD NFVVVYELLD EMMDFGYPQY TEAMILSEFI KTDAYRMEVT QRPPMAVTNA VSWRSEGIRY KKNEVFLDVV ESVNILVNSN GQIVRSDVIG
201: ELKMRTFLSG MPECKLGLND RVLLEAQGRT TKGKAIDLDD IKFHQCVRLA RFENDRTISF IPPDGSFDLM TYRLSTQVKP LIWVEAQVEK HSRSRIQITV
301: KTRSQFKERS TATNVEIEVP VPEDSTNPNI RTSMGSAAYA PERDAMVWKI KSFPGGKEYM CRAEFSLPSI TSEDGMPEKK APIRVKFEIP YFTVSGIQVR
401: YLKIIEKSGY QALPWVRYIT MAGEYELRLI

Best Arabidopsis Sequence Match ( AT1G60780.1 )
(BLAST)

001: MAGAASALFL LDIKGRVLVW RDYRGDVSAA QAERFFTKLI EKEGDSQSND PVAYDNGVTY MFVQHSNVYL MIASRQNCNA ASLLFFLHRV VDVFKHYFEE
101: LEEESLRDNF VVVYELLDEM MDFGYPQYTE ARILSEFIKT DAYRMEVTQR PPMAVTNAVS WRSEGIQYKK NEVFLDVIEN VNILVNSNGQ IVRSDVVGAL
201: KMRTYLTGMP ECKLGLNDRV LLEAQGRATK GKAIDLEDIK FHQCVRLARF ENDRTISFIP PDGAFDLMTY RLSTQVKPLI WVEAQIESHS RSRVEMLIKA
301: RSQFKERSTA TNVEIELPVP TDASNPTVRT SLGSASYAPE KDALVWKIKS FPGNKEYMLR AEFHLPSITA EEATPERKAP IRVKFEIPYF TVSGIQVRYL
401: KIIEKSGYQA LPWVRYITMA GEYELRLV

Arabidopsis Description

AP1M2AP-1 complex subunit mu-2 [Source:UniProtKB/Swiss-Prot;Acc:O22715]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.