Factsheet for Os08t0548900-01

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

nucleus 2
plastid 1
cytosol 4
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plastid: 15069638
plasma membrane: 27800704
plastid: 27992503

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 15069638 doi

N Tanaka, M Fujita, H Handa, S Murayama, M Uemura, Y Kawamura, T Mitsui, S Mikami, Y Tozawa, T Yoshinaga, S Komatsu

Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Japan.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0506100-00
Os03t0707600-01
Os04t0382800-00

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G51260.1	Os03t0707600-01	AT1G14920.1	21798944
AT3G51260.1	Os03t0707600-01	AT2G01570.1	21798944
AT3G51260.1	Os02t0506100-00	AT5G22310.1	21798944
AT3G51260.1	Os04t0382800-00	AT5G22310.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES15231	Sorghum	cytosol	97.19	97.19
Os09t0538200-01	Rice	cytosol, plastid	97.19	97.19
Zm00001d053217_P001	Maize	cytosol, extracellular	96.79	96.79
TraesCS7D01G272300.1	Wheat	cytosol	96.79	96.79
TraesCS7B01G170000.1	Wheat	cytosol, golgi, plastid, unclear	96.79	96.79
TraesCS7A01G272000.1	Wheat	cytosol	96.79	96.79
TraesCS5B01G329400.1	Wheat	cytosol	95.98	95.98
TraesCS5A01G329300.1	Wheat	cytosol	95.98	95.98
HORVU5Hr1G083350.1	Barley	cytosol	95.98	95.98
GSMUA_Achr11P...	Banana	cytosol	91.57	91.57
GSMUA_Achr2P03470_001	Banana	cytosol	91.57	91.57
KRH08047	Soybean	cytosol, endoplasmic reticulum, nucleus	91.57	91.57
VIT_03s0038g02300.t01	Wine grape	cytosol	91.16	91.16
KRH69759	Soybean	endoplasmic reticulum, nucleus	91.16	91.16
VIT_04s0023g03690.t01	Wine grape	cytosol	90.76	90.76
AT5G66140.1	Thale cress	cytosol	90.36	90.0
Bra012861.1-P	Field mustard	cytosol	89.56	89.2
Solyc01g111450.2.1	Tomato	plastid	89.16	89.16
PGSC0003DMT400015849	Potato	cytosol	89.16	89.16
AT3G51260.1	Thale cress	cytosol	89.16	88.8
CDX90655	Canola	cytosol	89.16	88.8
CDY29009	Canola	cytosol	89.16	88.8
Bra036824.1-P	Field mustard	cytosol	88.75	88.4
CDX77988	Canola	cytosol, extracellular	88.75	88.4
CDX73737	Canola	cytosol, extracellular	88.35	88.0
HORVU7Hr1G057390.2	Barley	cytosol, nucleus, plastid	95.98	84.15
CDX81411	Canola	mitochondrion, plastid	66.67	58.66
Os11t0615700-01	Rice	peroxisome	36.95	38.82
Os02t0634900-01	Rice	cytosol, plasma membrane	36.55	38.72
Os03t0387100-01	Rice	cytosol, plasma membrane	36.14	38.3
Os06t0177100-01	Rice	plastid	35.74	35.6
Os06t0176000-01	Rice	cytosol	35.74	35.6
Os06t0167600-01	Rice	cytosol	35.74	35.6
Os03t0180400-01	Rice	cytosol	34.94	35.37
Os05t0490800-01	Rice	cytosol, plasma membrane	30.92	30.92
Os01t0811100-01	Rice	cytosol	30.52	30.52
Os02t0133800-01	Rice	plastid	32.13	29.63
Os07t0614100-01	Rice	cytosol	27.31	26.88

Protein Annotations

MapMan:19.2.6.1.1.4	Gene3D:3.60.20.10	EntrezGene:4346245	EMBL:AK061808	ProteinID:BAD08948.1	ProteinID:BAD10760.1
ProteinID:BAF24351.1	ProteinID:BAT06571.1	ncoils:Coil	ProteinID:EEE54073.1	GO:GO:0000502	GO:GO:0003674
GO:GO:0003824	GO:GO:0004175	GO:GO:0004298	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005634	GO:GO:0005737	GO:GO:0005839	GO:GO:0006508	GO:GO:0006511	GO:GO:0008150
GO:GO:0008152	GO:GO:0008233	GO:GO:0009056	GO:GO:0009987	GO:GO:0016787	GO:GO:0019538
GO:GO:0019773	GO:GO:0051603	InterPro:IPR023332	InterPro:IPR029055	InterPro:Ntn_hydrolases_N	EnsemblPlantsGene:Os08g0548900
EnsemblPlants:Os08t0548900-01	PFAM:PF00227	PFAM:PF10584	ScanProsite:PS00388	PFscan:PS51475	PANTHER:PTHR11599
PANTHER:PTHR11599:SF106	InterPro:Proteasome_alpha-type	InterPro:Proteasome_asu_N	InterPro:Proteasome_sua/b	UniProt:Q6YT00	SMART:SM00948
SUPFAM:SSF56235	UniParc:UPI000016DED3	RefSeq:XP_015650855.1	SEG:seg	:	:

Description

Similar to Proteasome subunit alpha type 7 (EC 3.4.25.1) (20S proteasome alpha subunit D) (20S proteasome subunit alpha-4). (Os08t0548900-01)

Coordinates

chr8:+:27533329..27536724

Molecular Weight (calculated)

27297.5 Da

IEP (calculated)

7.563

GRAVY (calculated)

-0.373

Length

249 amino acids

Sequence
(BLAST)

001: MARYDRAITV FSPDGHLFQV EYALEAVRKG NAAVGVRGTD TVVLGVEKKS TPKLQDSRSM RKIASLDTHI ALACAGLKAD ARVLINRARV ECQSHRLTVE
101: DPVTVEYITR YIAGLQQKYT QSGGVRPFGL STLIVGFDPY TEKPALYQTD PSGTFSAWKA NATGRNSNSM REFLEKNYKD TSGKETIKLA IRALLEVVES
201: GGKNIEIAVM THKDGLRELE EAEIDEYVAE IEAEKAAAEA AKKGAPKET

Best Arabidopsis Sequence Match ( AT5G66140.1 )
(BLAST)

001: MARYDRAITV FSPDGHLFQV EYALEAVRKG NAAVGVRGTD TVVLAVEKKS TPKLQDSRSA RKIVSLDNHI ALACAGLKAD ARVLINKARI ECQSHRLTLE
101: DPVTVEYITR YIAGLQQKYT QSGGVRPFGL STLIVGFDPY SRLPSLYQTD PSGTFSAWKA NATGRNSNSI REFLEKNYKE SSGQETIKLA IRALLEVVES
201: GGKNIEVAVM TREETGLRQL EEAEIDAIVA KIEAEKAAAE AAKKGPPKET

Arabidopsis Description

PAD2Proteasome subunit alpha type-7-B [Source:UniProtKB/Swiss-Prot;Acc:O24616]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.