CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol, nucleus, endoplasmic reticulum

Predictor Summary:

nucleus 2
plastid 1
cytosol 4
mitochondrion 1

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 21107622
nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 21107622 doi

N Ahsan, T Nakamura, S Komatsu

National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH25284
KRH30384
KRH42544
KRH58636

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G51260.1	KRH42544	AT1G14920.1	21798944
AT3G51260.1	KRH58636	AT1G14920.1	21798944
AT3G51260.1	KRH42544	AT2G01570.1	21798944
AT3G51260.1	KRH58636	AT2G01570.1	21798944
AT3G51260.1	KRH25284	AT5G22310.1	21798944
AT3G51260.1	KRH30384	AT5G22310.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH69759	Soybean	endoplasmic reticulum, nucleus	98.39	98.39
VIT_03s0038g02300.t01	Wine grape	cytosol	95.18	95.18
PGSC0003DMT400015849	Potato	cytosol	94.38	94.38
Solyc01g111450.2.1	Tomato	plastid	94.38	94.38
VIT_04s0023g03690.t01	Wine grape	cytosol	94.38	94.38
GSMUA_Achr2P03470_001	Banana	cytosol	93.17	93.17
GSMUA_Achr11P...	Banana	cytosol	93.17	93.17
AT5G66140.1	Thale cress	cytosol	92.37	92.0
EER97100	Sorghum	cytosol	91.97	91.97
CDY29009	Canola	cytosol	91.97	91.6
Zm00001d022629_P001	Maize	cytosol	91.57	91.57
Os08t0548900-01	Rice	plastid	91.57	91.57
CDX77988	Canola	cytosol, extracellular	91.57	91.2
Bra036824.1-P	Field mustard	cytosol	91.57	91.2
Bra012861.1-P	Field mustard	cytosol	91.57	91.2
HORVU5Hr1G083350.1	Barley	cytosol	91.16	91.16
Zm00001d006235_P001	Maize	cytosol, extracellular	91.16	91.16
Os09t0538200-01	Rice	cytosol, plastid	91.16	91.16
TraesCS5B01G329400.1	Wheat	cytosol	91.16	91.16
TraesCS5A01G329300.1	Wheat	cytosol	91.16	91.16
CDX90655	Canola	cytosol	91.16	90.8
CDX73737	Canola	cytosol, extracellular	91.16	90.8
AT3G51260.1	Thale cress	cytosol	90.76	90.4
Zm00001d053217_P001	Maize	cytosol, extracellular	90.36	90.36
TraesCS7A01G272000.1	Wheat	cytosol	90.36	90.36
TraesCS7B01G170000.1	Wheat	cytosol, golgi, plastid, unclear	90.36	90.36
TraesCS7D01G272300.1	Wheat	cytosol	90.36	90.36
EES15231	Sorghum	cytosol	89.96	89.96
HORVU7Hr1G057390.2	Barley	cytosol, nucleus, plastid	89.56	78.52
CDX81411	Canola	mitochondrion, plastid	67.87	59.72
KRH57899	Soybean	cytosol	25.7	41.83
KRH65961	Soybean	cytosol	36.95	39.15
KRH43728	Soybean	cytosol, endoplasmic reticulum, nucleus	36.95	39.15
KRH75739	Soybean	cytosol, endoplasmic reticulum, nucleus	36.55	38.72
KRH13728	Soybean	cytosol	36.55	38.72
KRH69234	Soybean	endoplasmic reticulum	36.55	38.4
KRG90710	Soybean	peroxisome	36.55	38.4
KRH36037	Soybean	endoplasmic reticulum, nucleus	36.55	38.4
KRH31817	Soybean	cytosol	36.14	37.97
KRH26034	Soybean	nucleus	35.74	36.18
KRH55422	Soybean	cytosol, endoplasmic reticulum	35.74	36.18
KRH00322	Soybean	cytosol	35.34	35.2
KRH49455	Soybean	endoplasmic reticulum, nucleus	34.94	34.8
KRH76017	Soybean	cytosol	34.94	34.8
KRH65617	Soybean	nucleus	34.94	34.8
KRH05846	Soybean	cytosol, nucleus	32.13	28.17
KRG94269	Soybean	cytosol	28.11	28.11
KRH38836	Soybean	cytosol, nucleus	32.13	28.07
KRH15173	Soybean	cytosol, nucleus	32.13	27.78
KRH57704	Soybean	cytosol, endoplasmic reticulum	26.1	24.9
KRH20407	Soybean	cytosol	12.85	20.91

Protein Annotations

EntrezGene:100790481	MapMan:19.2.6.1.1.4	Gene3D:3.60.20.10	EMBL:ACUP02010137	ncoils:Coil	EnsemblPlantsGene:GLYMA_16G126200
GO:GO:0000502	GO:GO:0003674	GO:GO:0003824	GO:GO:0004175	GO:GO:0004298	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005737	GO:GO:0005839	GO:GO:0006508
GO:GO:0006511	GO:GO:0008150	GO:GO:0008152	GO:GO:0008233	GO:GO:0009056	GO:GO:0009987
GO:GO:0016787	GO:GO:0019538	GO:GO:0019773	GO:GO:0051603	UniProt:I1MN39	InterPro:IPR023332
InterPro:IPR029055	EnsemblPlants:KRH08047	ProteinID:KRH08047	ProteinID:KRH08047.1	InterPro:Ntn_hydrolases_N	PFAM:PF00227
PFAM:PF10584	ScanProsite:PS00388	PFscan:PS51475	PANTHER:PTHR11599	PANTHER:PTHR11599:SF106	InterPro:Proteasome_alpha-type
InterPro:Proteasome_asu_N	InterPro:Proteasome_sua/b	SMART:SM00948	SUPFAM:SSF56235	MEROPS:T01.974	UniParc:UPI000233B7A0
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr16:-:27742819..27746406

Molecular Weight (calculated)

27194.3 Da

IEP (calculated)

7.566

GRAVY (calculated)

-0.322

Length

249 amino acids

Sequence
(BLAST)

001: MARYDRAITV FSPDGHLFQV EYALEAVRKG NAAVGVRGTD NVVLGVEKKS TAKLQDSRSV RKIVNLDDHI ALACAGLKAD ARVLINRARV ECQSHRLTVE
101: DPVTVEYITR YIAGLQQKYT QSGGVRPFGL STLIVGFDPY THSPSLYQTD PSGTFSAWKA NATGRNSNSI REFLEKNYKD TSGQETVKLA IRALLEVVES
201: GGKNIEVAVM TKEQGLRQLE EAEIDAIVAE IEAEKAAAEA AKKAPPKET

Best Arabidopsis Sequence Match ( AT5G66140.1 )
(BLAST)

001: MARYDRAITV FSPDGHLFQV EYALEAVRKG NAAVGVRGTD TVVLAVEKKS TPKLQDSRSA RKIVSLDNHI ALACAGLKAD ARVLINKARI ECQSHRLTLE
101: DPVTVEYITR YIAGLQQKYT QSGGVRPFGL STLIVGFDPY SRLPSLYQTD PSGTFSAWKA NATGRNSNSI REFLEKNYKE SSGQETIKLA IRALLEVVES
201: GGKNIEVAVM TREETGLRQL EEAEIDAIVA KIEAEKAAAE AAKKGPPKET

Arabidopsis Description

PAD2Proteasome subunit alpha type-7-B [Source:UniProtKB/Swiss-Prot;Acc:O24616]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.