CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, endoplasmic reticulum

Predictor Summary:

nucleus 2
mitochondrion 1
peroxisome 4
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 23041469
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH10294
KRH23029
KRH24840
KRH29844

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G53850.1	KRH10294	AT3G46090.1	17259181
AT1G53850.1	KRH23029	AT3G46090.1	17259181
AT1G53850.1	KRH24840	AT3G46090.1	17259181
AT1G53850.1	KRH29844	AT3G46090.1	17259181

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG90710	Soybean	peroxisome	100.0	100.0
KRH69234	Soybean	endoplasmic reticulum	99.16	99.16
KRH31817	Soybean	cytosol	99.16	99.16
GSMUA_Achr11P...	Banana	peroxisome	97.89	97.89
PGSC0003DMT400054523	Potato	cytosol	97.47	97.47
Solyc02g070510.2.1	Tomato	nucleus	97.47	97.47
PGSC0003DMT400024982	Potato	cytosol, nucleus, peroxisome	96.62	96.62
Solyc08g016510.2.1	Tomato	extracellular	96.62	96.62
AT3G14290.1	Thale cress	cytosol	96.2	96.2
Os11t0615700-01	Rice	peroxisome	96.2	96.2
Zm00001d027688_P002	Maize	peroxisome	96.2	96.2
EES10115	Sorghum	peroxisome	96.2	96.2
AT1G53850.1	Thale cress	cytosol	95.78	95.78
OQU93221	Sorghum	cytosol	95.78	95.78
Bra030921.1-P	Field mustard	cytosol	95.78	95.78
TraesCS5B01G114000.1	Wheat	golgi, plastid, unclear	95.36	95.36
TraesCS4A01G167300.1	Wheat	peroxisome	94.51	94.51
HORVU4Hr1G040770.1	Barley	cytosol	94.09	94.09
TraesCS4B01G151700.1	Wheat	golgi, unclear	94.09	94.09
TraesCS4D01G157700.1	Wheat	peroxisome	94.09	94.09
CDY08208	Canola	peroxisome	94.51	91.8
CDY51800	Canola	peroxisome	94.51	89.6
TraesCS5D01G121300.1	Wheat	peroxisome	88.19	88.19
TraesCS5A01G109000.1	Wheat	peroxisome	71.31	87.11
CDY02493	Canola	mitochondrion	95.78	81.07
CDY25683	Canola	cytosol, plastid	95.78	80.21
VIT_09s0002g00350.t01	Wine grape	endoplasmic reticulum	98.31	79.52
Zm00001d048403_P001	Maize	extracellular	95.36	77.13
KRH00322	Soybean	cytosol	40.51	38.4
KRH76017	Soybean	cytosol	40.51	38.4
KRH65617	Soybean	nucleus	40.51	38.4
KRH49455	Soybean	endoplasmic reticulum, nucleus	40.51	38.4
KRH69759	Soybean	endoplasmic reticulum, nucleus	39.24	37.35
KRH08047	Soybean	cytosol, endoplasmic reticulum, nucleus	38.4	36.55
KRH43728	Soybean	cytosol, endoplasmic reticulum, nucleus	33.33	33.62
KRH13728	Soybean	cytosol	33.33	33.62
KRH65961	Soybean	cytosol	32.91	33.19
KRH75739	Soybean	cytosol, endoplasmic reticulum, nucleus	32.91	33.19
KRH05846	Soybean	cytosol, nucleus	37.13	30.99
KRH38836	Soybean	cytosol, nucleus	36.71	30.53
KRH15173	Soybean	cytosol, nucleus	36.71	30.21
KRH55422	Soybean	cytosol, endoplasmic reticulum	30.8	29.67
KRH26034	Soybean	nucleus	30.8	29.67
KRG94269	Soybean	cytosol	30.8	29.32
KRH57704	Soybean	cytosol, endoplasmic reticulum	30.38	27.59
KRH57899	Soybean	cytosol	14.35	22.22
KRH20407	Soybean	cytosol	13.08	20.26

Protein Annotations

EntrezGene:100796147	EntrezGene:100799919	MapMan:19.2.6.1.1.5	Gene3D:3.60.20.10	EMBL:ACUP02006718	EMBL:ACUP02012633
EMBL:BT096849	UniProt:C6TGU2	EnsemblPlantsGene:GLYMA_10G279900	GO:GO:0000502	GO:GO:0003674	GO:GO:0003824
GO:GO:0004175	GO:GO:0004298	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005737	GO:GO:0005839	GO:GO:0006508	GO:GO:0006511	GO:GO:0008150	GO:GO:0008152
GO:GO:0008233	GO:GO:0009056	GO:GO:0009987	GO:GO:0016787	GO:GO:0019538	GO:GO:0019773
GO:GO:0043161	GO:GO:0051603	InterPro:IPR023332	InterPro:IPR029055	ProteinID:KRG90710.1	EnsemblPlants:KRH36037
ProteinID:KRH36037	ProteinID:KRH36037.1	InterPro:Ntn_hydrolases_N	PFAM:PF00227	PFAM:PF10584	ScanProsite:PS00388
PFscan:PS51475	PANTHER:PTHR11599	PANTHER:PTHR11599:SF14	InterPro:Proteasome_alpha-type	InterPro:Proteasome_alpha_type_5	InterPro:Proteasome_asu_N
InterPro:Proteasome_sua/b	SMART:SM00948	SUPFAM:SSF56235	MEROPS:T01.995	UniParc:UPI0001B16CC4	:

Description

hypothetical protein

Coordinates

chr10:+:50130564..50136128

Molecular Weight (calculated)

25997.7 Da

IEP (calculated)

4.410

GRAVY (calculated)

-0.240

Length

237 amino acids

Sequence
(BLAST)

001: MFLTRTEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGL KTKEGVVLAV EKRITSPLLE PSSVEKIMEI DEHIGCAMSG LIADARTLVE HARVETQNHR
101: FSYGEPMTVE STTQALCDLA LRFGEGDEES MSRPFGVSLL IAGHDENGPS LYYTDPSGTF WQCNGKAIGS GSEGADSSLQ EQYNKDLTLQ EAETIALSIL
201: KQVMEEKVTP NNVDIAKVAP TYHLYTPSEV EAVISRL

Best Arabidopsis Sequence Match ( AT3G14290.1 )
(BLAST)

001: MFLTRTEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGV KTKEGVVLAV EKRITSPLLE PSSVEKIMEI DDHIGCAMSG LIADARTLVE HARVETQNHR
101: FSYGEPMTVE STTQALCDLA LRFGEGEEES MSRPFGVSLL IAGHDENGPS LYYTDPSGTF WQCNAKAIGS GSEGADSSLQ EQFNKDITLQ EAETIAVSIL
201: KQVMEEKVTP NNVDIAKVAP AYHLYTPQEV EAVISRL

Arabidopsis Description

PAE2Proteasome subunit alpha type-5-B [Source:UniProtKB/Swiss-Prot;Acc:Q42134]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.