Factsheet for Os08t0550100-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 4
cytosol 1
mitochondrion 1
plastid 2

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plastid: 27992503
plasma membrane: 28056797

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os04t0550400-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G75990.1	Os04t0550400-01	AT2G44950.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES14113	Sorghum	cytosol, nucleus, plastid	94.44	92.54
Os09t0541900-01	Rice	cytosol, nucleus, plastid	91.36	91.17
TraesCS4B01G265700.1	Wheat	plastid	91.36	90.98
TraesCS4A01G044600.1	Wheat	golgi	91.15	90.41
TraesCS4D01G265700.1	Wheat	unclear	90.74	90.37
TraesCS4B01G265800.1	Wheat	plastid	90.74	90.37
Zm00001d053232_P005	Maize	cytosol, nucleus, plastid	94.24	90.16
HORVU4Hr1G071480.8	Barley	plastid	90.74	89.09
Zm00001d032010_P007	Maize	cytosol, nucleus, plastid	93.83	87.36
KRH61001	Soybean	nucleus	80.66	80.49
KRH51666	Soybean	nucleus	80.04	79.88
GSMUA_Achr11P...	Banana	cytosol, nucleus, plastid	79.84	79.51
VIT_18s0001g01120.t01	Wine grape	nucleus	79.22	79.06
VIT_03s0038g02590.t01	Wine grape	plastid	78.81	78.64
PGSC0003DMT400015774	Potato	nucleus	79.84	78.54
KRH17475	Soybean	nucleus	78.81	78.16
KRH05990	Soybean	cytosol, nucleus, plastid	76.75	76.12
AT1G20200.1	Thale cress	cytosol	76.34	76.02
AT1G75990.1	Thale cress	cytosol	75.31	75.15
GSMUA_Achr8P13080_001	Banana	cytosol, extracellular, plastid	41.15	73.8
GSMUA_Achr5P18970_001	Banana	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	79.84	70.55
HORVU7Hr1G080070.2	Barley	cytosol	13.58	68.75
GSMUA_Achr8P13090_001	Banana	cytosol	33.54	67.36
PGSC0003DMT400015775	Potato	endoplasmic reticulum, golgi, plasma membrane	62.35	62.35
CDY01387	Canola	cytosol	19.96	45.33
Bra012219.1-P	Field mustard	cytosol	19.96	45.33
Solyc10g044990.1.1	Tomato	cytosol, nucleus, peroxisome	9.67	30.72

Protein Annotations

Gene3D:1.25.40.570	MapMan:19.2.6.2.2.3	InterPro:26S_Psome_reg_C	EntrezGene:4346252	ProteinID:BAD08959.1	ProteinID:BAF24358.1
ProteinID:BAT06580.1	ncoils:Coil	GO:GO:0000502	GO:GO:0003674	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0006511	GO:GO:0008150	GO:GO:0008152	GO:GO:0008541	GO:GO:0009056
GO:GO:0009987	GO:GO:0019538	GO:GO:0030234	GO:GO:0042176	GO:GO:0050790	InterPro:IPR000717
EnsemblPlantsGene:Os08g0550100	EnsemblPlants:Os08t0550100-01	InterPro:PCI_dom	PFAM:PF01399	PFAM:PF08375	PFscan:PS50250
PANTHER:PTHR10758	PANTHER:PTHR10758:SF7	UniProt:Q6ZJI3	SMART:SM00088	SMART:SM00753	SUPFAM:SSF46785
UniParc:UPI000023AC8C	InterPro:WH_DNA-bd_sf	RefSeq:XP_015650753.1	SEG:seg	:	:

Description

19S REGULATORY PARTICLE NON-ATPASE SUBUNIT 3Similar to 26S proteasome subunit RPN3a. (Os08t0550100-01);Non-protein coding transcript. (Os08t0550100-02)

Coordinates

chr8:-:27588536..27592035

Molecular Weight (calculated)

55200.3 Da

IEP (calculated)

8.625

GRAVY (calculated)

-0.293

Length

486 amino acids

Sequence
(BLAST)

001: MTDVQMSDSE PQAAPPAAPA ASALHHLKEI ASVIEAGSLS KEVRRISRAV RLTVALRRRL AARDVSAFLA FALPPSSEAY TRLTSLVPKE DDTEMDIDMA
101: APATQISIKH GLPEIEIYCY LLVLIFLIDQ KKYDDAKACA NASIARLKNL NRRTVDVLAS RLYFYYSYVH ELTNSLAEIR GNLLALHRMA TLHRDELGQE
201: TLLNLLLRNY LHYNLYDQAE KLRSKAPRFE AHSNQQFCRY LFYLGKIRTI QLEYTDAKES LLQAARKAPI TARGFRIQCN KWAIIVRLLL GEIPERTVFM
301: QKGMKEALTP YFELTNAVRI GDLELFRAVA DKFVSTFSAD RTRNLIVRLR HNVIRTGLRN ISISYSRISL ADIAKKLRLD SDNPVADAES IVAKAIRDGA
401: IDATIDHANG WMVSKETGDV YSTNEPQIAF NSRIAFCLNM HNEAVKAMRF PPNSHKEKES AEKRRERLQQ EEELAKHMAE EDDDDF

Best Arabidopsis Sequence Match ( AT1G20200.1 )
(BLAST)

001: MTQDVEMKDN NTPSQSIISS STSTMQNLKE IAALIDTGSY TKEVRRIARA VRLTIGLRQK LTGSVLSSFL DFALVPGSEA HSRLSSFVPK GDEHDMEVDT
101: ASSATQAAPS KHLPAELEIY CYFIVLLFLI DQKKYNEAKA CSSASIARLK NVNRRTIDVI ASRLYFYYSL SYEQTGDLAE IRGTLLALHH SATLRHDELG
201: QETLLNLLLR NYLHYNLYDQ AEKLRSKAPR FEAHSNQQFC RYLFYLGKIR TIQLEYTDAK ESLLQAARKA PIAALGFRIQ CNKWAILVRL LLGEIPERSI
301: FTQKGMEKAL RPYFELTNAV RIGDLELFRT VQEKFLDTFA QDRTHNLIVR LRHNVIRTGL RNISISYSRI SLPDVAKKLR LNSENPVADA ESIVAKAIRD
401: GAIDATIDHK NGCMVSKETG DIYSTNEPQT AFNSRIAFCL NMHNEAVRAL RFPPNTHKEK ESDEKRRERK QQEEELAKHM AEEDDDDF

Arabidopsis Description

RPN3A26S proteasome non-ATPase regulatory subunit 3 homolog A [Source:UniProtKB/Swiss-Prot;Acc:Q9LNU4]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.