Factsheet for Zm00001d042645_P001

Subcellular Localization

min:

: max

Winner_takes_all: peroxisome, nucleus, cytosol

Predictor Summary:

peroxisome 1
mitochondrion 1
nucleus 1
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, nucleus, peroxisome
Any Predictor:	cytosol, mitochondrion, peroxisome
MultiLoc:	peroxisome
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Zm00001d007384_P003
Zm00001d008906_P003
Zm00001d012342_P001
Zm00001d015036_P005
Zm00001d018004_P002
Zm00001d024153_P001
Zm00001d025835_P001
Zm00001d025836_P001
Zm00001d028803_P002
Zm00001d029004_P004
Zm00001d032131_P001
Zm00001d035534_P003
Zm00001d035539_P001
Zm00001d038664_P001
Zm00001d042645_P001
Zm00001d044802_P001
Zm00001d046856_P002
Zm00001d047540_P001
Zm00001d047701_P002
Zm00001d052273_P004
Zm00001d053899_P001

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G16340.1	Zm00001d015036_P005	AT1G20090.1	17267444
AT4G16340.1	Zm00001d053899_P001	AT1G20090.1	17267444
AT4G16340.1	Zm00001d008906_P003	AT1G29170.1	17267444
AT4G16340.1	Zm00001d025835_P001	AT1G29170.1	17267444
AT4G16340.1	Zm00001d025836_P001	AT1G29170.1	17267444
AT4G16340.1	Zm00001d032131_P001	AT1G29170.1	17267444
AT4G16340.1	Zm00001d028803_P002	AT2G38440.1	17267444
AT4G16340.1	Zm00001d047701_P002	AT2G38440.1	17267444
AT4G16340.1	Zm00001d007384_P003	AT4G16340.1	17267444
AT4G16340.1	Zm00001d012342_P001	AT4G16340.1	17267444
AT4G16340.1	Zm00001d024153_P001	AT4G16340.1	17267444
AT4G16340.1	Zm00001d029004_P004	AT4G16340.1	17267444
AT4G16340.1	Zm00001d035534_P003	AT4G16340.1	17267444
AT4G16340.1	Zm00001d035539_P001	AT4G16340.1	17267444
AT4G16340.1	Zm00001d038664_P001	AT4G16340.1	17267444
AT4G16340.1	Zm00001d042645_P001	AT4G16340.1	17267444
AT4G16340.1	Zm00001d046856_P002	AT4G16340.1	17267444
AT4G16340.1	Zm00001d047540_P001	AT4G16340.1	17267444
AT4G16340.1	Zm00001d028803_P002	AT5G01730.1	17267444
AT4G16340.1	Zm00001d047701_P002	AT5G01730.1	17267444
AT4G16340.1	Zm00001d018004_P002	AT5G62880.1	17267444
AT4G16340.1	Zm00001d044802_P001	AT5G62880.1	17267444
AT4G16340.1	Zm00001d052273_P004	AT5G62880.1	17267444

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d012342_P001	Maize	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	38.67	97.84
Zm00001d024153_P001	Maize	cytosol	24.53	94.74
Zm00001d035539_P001	Maize	cytosol	25.04	71.71
Solyc08g016070.1.1	Tomato	mitochondrion	40.37	38.6
Zm00001d007384_P003	Maize	cytosol, endoplasmic reticulum, peroxisome	16.01	26.26
Zm00001d047540_P001	Maize	cytosol, mitochondrion	64.05	20.37
Zm00001d029004_P004	Maize	cytosol	59.63	18.77
Zm00001d035534_P003	Maize	cytosol	13.8	17.12
Zm00001d046856_P002	Maize	endoplasmic reticulum, nucleus, peroxisome	14.14	15.57
Zm00001d038664_P001	Maize	cytosol, nucleus, plastid	13.12	14.39
VIT_11s0016g00100.t01	Wine grape	mitochondrion	43.44	13.81
AT4G16340.1	Thale cress	cytosol	41.74	13.39
KRH43411	Soybean	endoplasmic reticulum	40.37	12.92
CDY32235	Canola	cytosol	40.03	12.88
KRH13046	Soybean	endoplasmic reticulum	40.03	12.81
CDY34451	Canola	endoplasmic reticulum	40.37	12.55
Bra038448.1-P	Field mustard	cytosol	40.03	11.34
TraesCS5A01G195400.1	Wheat	cytosol	1.36	5.88
TraesCS5D01G127700.1	Wheat	cytosol	0.51	2.1
Solyc08g016050.2.1	Tomato	nucleus	1.19	0.77
Solyc08g016060.1.1	Tomato	extracellular	0.34	0.7

Protein Annotations

KEGG:00230+3.6.1.66	Gene3D:3.90.950.10	MapMan:35.1	UniProt:A0A1D6N5R7	ncoils:Coil	InterPro:DHR-1_domain
InterPro:DUF966	GO:GO:0003674	GO:GO:0003824	GO:GO:0006139	GO:GO:0008150	GO:GO:0008152
GO:GO:0009056	GO:GO:0009058	GO:GO:0009143	GO:GO:0009987	GO:GO:0016787	GO:GO:0047429
InterPro:Ham1p-like	InterPro:IPR027007	InterPro:IPR029001	InterPro:ITPase-like_fam	ProteinID:ONM35965.1	PFAM:PF01725
PFAM:PF06136	PFscan:PS51650	PANTHER:PTHR31083	PANTHER:PTHR31083:SF8	SUPFAM:SSF52972	UniParc:UPI000844DF2E
EnsemblPlantsGene:Zm00001d042645	EnsemblPlants:Zm00001d042645_P001	EnsemblPlants:Zm00001d042645_T001	SEG:seg	:	:

Description

Guanine nucleotide exchange factor SPIKE 1

Coordinates

chr3:+:175239376..175247569

Molecular Weight (calculated)

65262.2 Da

IEP (calculated)

7.585

GRAVY (calculated)

-0.327

Length

587 amino acids

Sequence
(BLAST)

001: MTDSFRILGE EDVGARTCPK LRPVSVLMEL VTCGSLSVKD HGNAGVVQTC KPRFPNLRFL PSPLISRTMM MGELDCLSEN PKLMGIKQKV LIVQGKIVPA
101: RGPNDFGWDP VFQSDCFEQT YDEMPKLVKN EISHRGKALA LGVVILVQYN IAKYKVEGVS KKRRDIRGGG GSQPSSSRTD RGLSVDFKEP KVAEFDDLEE
201: DKFLNVVVKH CSLSPLPAYE PAFDWENERS LIFGQRVPES IPAISNSGLK ITIKVLSLSF QAGLVEPFSG TISLYNRDRR EKLSEDFYFH ILPTDMQDAA
301: TKEGGVTPSV YSRKDPVHLA EKEKQKLQVW SRIMPCKESF AWAMIPLFEG NHAGGLSDAA YPSSPLATSL PGSTSQDSIV DPILKLTLDG KVNHYSSGSS
401: VIVEISNLNK VKESYIVDSL QDPKRKVHKP VKGVLRLEVE KLHGGHNDVD NTSEGGSMAN DLNDAGDINN GRSNRSCFDG IHSFMNSIAI AQKDAHHNGI
501: ISNAENGDNR RCTFLLQFEA FDFRMLTRSE PFSQLFHCLY VYPLTVSLSR KRNLFVRVEL RKDDSDIRKP PLEVIVGHSV LPLSTHI

Best Arabidopsis Sequence Match ( AT4G16340.1 )
(BLAST)

0001: MENNNLGLRF RKLPRQPLAL PKLDPLLDEN LEQWPHLNQL VQCYGTEWVK DVNKYGHYEN IRPDSFQTQI FEGPDTDTET EIRLASARSA TIEEDVASIS
0101: GRPFSDPGSS KHFGQPPLPA YEPAFDWENE RAMIFGQRTP ESPAASYSSG LKISVRVLSL AFQSGLVEPF FGSIALYNQE RKEKLSEDFY FQIQPTEMQD
0201: AKLSSENRGV FYLDAPSASV CLLIQLEKTA TEEGGVTSSV YSRKEPVHLT EREKQKLQVW SRIMPYRESF AWAVVPLFDN NLTTNTGESA SPSSPLAPSM
0301: TASSSHDGVY EPIAKITSDG KQGYSGGSSV VVEISNLNKV KESYSEESIQ DPKRKVHKPV KGVLRLEIEK HRNGHGDFED LSENGSIIND SLDPTDRLSD
0401: LTLMKCPSSS SGGPRNGCSK WNSEDAKDVS RNLTSSCGTP DLNCYHAFDF CSTTRNEPFL HLFHCLYVYP VAVTLSRKRN PFIRVELRKD DTDIRKQPLE
0501: AIYPREPGVS LQKWVHTQVA VGARAASYHD EIKVSLPATW TPSHHLLFTF FHVDLQTKLE APRPVVVGYA SLPLSTYIHS RSDISLPVMR ELVPHYLQES
0601: TKERLDYLED GKNIFKLRLR LCSSLYPTNE RVRDFCLEYD RHTLQTRPPW GSELLQAINS LKHVDSTALL QFLYPILNML LHLIGNGGET LQVAAFRAMV
0701: DILTRVQQVS FDDADRNRFL VTYVDYSFDD FGGNQPPVYP GLATVWGSLA RSKAKGYRVG PVYDDVLSMA WFFLELIVKS MALEQARLYD HNLPTGEDVP
0801: PMQLKESVFR CIMQLFDCLL TEVHERCKKG LSLAKRLNSS LAFFCYDLLY IIEPCQVYEL VSLYMDKFSG VCQSVLHECK LTFLQIISDH DLFVEMPGRD
0901: PSDRNYLSSI LIQELFLSLD HDELPLRAKG ARILVILLCK HEFDARYQKA EDKLYIAQLY FPFVGQILDE MPVFYNLNAT EKREVLIGVL QIVRNLDDTS
1001: LVKAWQQSIA RTRLYFKLME ECLILFEHKK AADSILGGNN SRGPVSEGAG SPKYSERLSP AINNYLSEAS RQEVRLEGTP DNGYLWQRVN SQLASPSQPY
1101: SLREALAQAQ SSRIGASAQA LRESLHPILR QKLELWEENV SATVSLQVLE ITENFSSMAA SHNIATDYGK LDCITTILTS FFSRNQSLAF WKAFFPIFNR
1201: IFDLHGATLM ARENDRFLKQ IAFHLLRLAV YRNDSVRKRA VIGLQILVKS SLYFMQTARL RALLTITLSE LMSDVQVTHM KSDNTLEESG EARRLQQSLS
1301: EMADEAKSVN LLRECGLPDD TLLIIPEKFT ENRWSWAEVK HLSDSLVLAL DASLGHALLG SVMAMDRYAA AESFYKLGMA FAPVPDLHIM WLLHLCDAHQ
1401: EMQSWAEAAQ CAVAVAGVIM QALVARNDGV WSKDHVSALR KICPMVSGEF TTEASAAEVE GYGASKLTVD SAVKYLQLAN KLFSQAELYH FCASILELVI
1501: PVYKSRKAYG QLAKCHTLLT NIYESILDQE SNPIPFIDAT YYRVGFYGEK FGKLDRKEYV YREPRDVRLG DIMEKLSHIY ESRMDSNHIL HIIPDSRQVK
1601: AEDLQAGVCY LQITAVDAVM EDEDLGSRRE RIFSLSTGSV RARVFDRFLF DTPFTKNGKT QGGLEDQWKR RTVLQTEGSF PALVNRLLVT KSESLEFSPV
1701: ENAIGMIETR TTALRNELEE PRSSDGDHLP RLQSLQRILQ GSVAVQVNSG VLSVCTAFLS GEPATRLRSQ ELQQLIAALL EFMAVCKRAI RVHFRLIGEE
1801: DQEFHTQLVN GFQSLTAELS HYIPAILSEL

Arabidopsis Description

SPK1Guanine nucleotide exchange factor SPIKE 1 [Source:UniProtKB/Swiss-Prot;Acc:Q8SAB7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.