Subcellular Localization
min:
: max
Winner_takes_all: cytosol
Predictor Summary:
Predictor Summary:
- nucleus 3
- cytosol 2
- mitochondrion 1
- plasma membrane 3
- plastid 1
- extracellular 2
- endoplasmic reticulum 2
- vacuole 2
- golgi 2
| Predictors | GFP | MS/MS | Papers | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
PPI
Inferred distinct locusB in Crop
Inferred from Arabidopsis experimental PPI
Homology
Paralog
| locus | Identity | Homology Identity |
|---|
Ortholog
| locus | Homology Species | Location | Identity | Homology Identity |
|---|---|---|---|---|
| CDY32235 | Canola | cytosol | 86.82 | 98.63 |
| AT4G16340.1 | Thale cress | cytosol | 83.06 | 94.04 |
| Os03t0328000-01 | Rice | plasma membrane | 12.74 | 93.95 |
| GSMUA_Achr10P... | Banana | extracellular | 42.91 | 83.79 |
| Solyc08g016050.2.1 | Tomato | nucleus | 36.34 | 83.11 |
| Solyc08g016060.1.1 | Tomato | extracellular | 11.34 | 82.46 |
| VIT_11s0016g00100.t01 | Wine grape | mitochondrion | 72.25 | 81.05 |
| KRH43411 | Soybean | endoplasmic reticulum | 70.17 | 79.24 |
| KRH13046 | Soybean | endoplasmic reticulum | 69.79 | 78.8 |
| KXG39465 | Sorghum | cytosol | 66.07 | 74.44 |
| HORVU4Hr1G051250.3 | Barley | cytosol | 65.59 | 74.1 |
| TraesCS4A01G131100.1 | Wheat | cytosol | 65.44 | 73.9 |
| TraesCS4B01G173500.4 | Wheat | cytosol | 65.44 | 73.9 |
| TraesCS4D01G175600.4 | Wheat | cytosol | 65.25 | 73.68 |
| Zm00001d047540_P001 | Maize | cytosol, mitochondrion | 65.3 | 73.29 |
| Zm00001d029004_P004 | Maize | cytosol | 65.73 | 73.03 |
| Solyc08g016070.1.1 | Tomato | mitochondrion | 19.21 | 64.82 |
| GSMUA_Achr10P... | Banana | cytosol | 24.52 | 62.87 |
| Zm00001d024153_P001 | Maize | cytosol | 4.15 | 56.58 |
| Zm00001d035539_P001 | Maize | cytosol | 5.12 | 51.71 |
| Zm00001d012342_P001 | Maize | cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole | 5.6 | 50.0 |
| Zm00001d042645_P001 | Maize | cytosol, nucleus, peroxisome | 11.34 | 40.03 |
| TraesCS5A01G195400.1 | Wheat | cytosol | 2.41 | 36.76 |
| TraesCS5D01G127700.1 | Wheat | cytosol | 2.46 | 35.66 |
| Zm00001d007384_P003 | Maize | cytosol, endoplasmic reticulum, peroxisome | 5.6 | 32.4 |
| Zm00001d035534_P003 | Maize | cytosol | 6.52 | 28.54 |
| Zm00001d046856_P002 | Maize | endoplasmic reticulum, nucleus, peroxisome | 7.19 | 27.95 |
| Zm00001d038664_P001 | Maize | cytosol, nucleus, plastid | 6.52 | 25.23 |
Protein Annotations
| Gene3D:1.10.238.10 | Gene3D:1.20.58.740 | Gene3D:1.25.40.410 | MapMan:27.4.3.2 | EnsemblPlantsGene:Bra038448 | EnsemblPlants:Bra038448.1 |
| EnsemblPlants:Bra038448.1-P | ncoils:Coil | InterPro:DHR-1_domain | InterPro:DHR-2 | InterPro:DOCK | InterPro:DOCK_C |
| InterPro:EF-hand-dom_pair | InterPro:EF_hand_dom | GO:GO:0003674 | GO:GO:0005085 | GO:GO:0005089 | GO:GO:0005488 |
| GO:GO:0005509 | GO:GO:0005515 | GO:GO:0005575 | GO:GO:0005622 | GO:GO:0005623 | GO:GO:0005634 |
| GO:GO:0005737 | GO:GO:0005783 | GO:GO:0005829 | GO:GO:0005886 | GO:GO:0006810 | GO:GO:0007154 |
| GO:GO:0007165 | GO:GO:0007264 | GO:GO:0008064 | GO:GO:0008150 | GO:GO:0008360 | GO:GO:0009605 |
| GO:GO:0009606 | GO:GO:0009628 | GO:GO:0009653 | GO:GO:0009719 | GO:GO:0009958 | GO:GO:0009987 |
| GO:GO:0010928 | GO:GO:0016020 | GO:GO:0016043 | GO:GO:0016192 | GO:GO:0019898 | GO:GO:0051493 |
| GO:GO:0065009 | GO:GO:0070971 | InterPro:IPR002048 | InterPro:IPR027007 | InterPro:IPR027357 | UniProt:M4FBI2 |
| PFAM:PF06920 | PFAM:PF14429 | PFscan:PS50222 | PFscan:PS51650 | PFscan:PS51651 | PANTHER:PTHR23317 |
| PANTHER:PTHR23317:SF76 | SMART:SM00054 | SUPFAM:SSF47473 | UniParc:UPI00025446EA | SEG:seg | : |
Description
AT4G16340 (E=0.0) SPK1 | SPK1 (SPIKE1); GTP binding / GTPase binding / guanyl-nucleotide exchange factor
Coordinates
chrA01:+:11150415..11164186
Molecular Weight (calculated)
234262.0 Da
IEP (calculated)
6.263
GRAVY (calculated)
-0.216
Length
2072 amino acids
Sequence
(BLAST)
(BLAST)
0001: MMMQCLDALK HFSIFILTCC DSDLHHHSRG LPNPKDLAKG TVFTVNEIEA LYEMFKSISK AGLIDKEQFQ LVLFKSNRKK SLFADRVFDL FDTKHTGILD
0101: FETFARAISV FHPKARMEDK LEFAFKLYDL KHQGFIERQE LRQMMVATLA ESGMNLSDGV IEIIIDKIDK EEWQSLVLRH PSLLQNMTLQ HLNVKRIRLV
0201: LFLFSRMECN EQDWNQGYLS TNSPNVLPFP LPSSSDAVAD ISPISKPWRT IAFASARFLG SLFLSPSSNL WYLDGNLEQW PHLNQLVQCY GTEWIKDANK
0301: YGHYDTTRPD TFQTQIFEGP DTDTETEFRL ASARSATLDE DVASVSGTPF TDSGSPKHFG LPPLPAYEPA FDWENERSMI CGQRTPESPA ASYSSGLKIS
0401: VRVLSLAFQS GLVEPFYGSI SLYNQERKEK LSEDFYFHIS PTEMQDAKPS SENRGVFYLD APSASVCLLI QLEKTATEEG GVTTSVYSRK EPVHLTEREK
0501: QKLQVWSRIM PYRESFAWAV VPLFDNSITT NTGESASPSS PLAPSMTASS SHDGVFEPMA KITSDGKQGS SGGSSVVVEI SNLNKVKENY SEESIQDPKR
0601: KVHKPVKGVL RLEIEKHRNG HGDVEDLSEN GSIRNESLDL TDRLGDLTLM KCPSAGSGGP HNSGSKWSTE DVSKNLTSSS GNVDNCYYAF DFCSTTRNEP
0701: FLHLFHCLYV YPVAVTLSRK RNPFIRVELR KDDTDVRKQP LEALYPREPG VSLQKWAHTQ VAVGARAASY HDEIKVSLPA TWTPSHHLLF TFFHVDLQTK
0801: LEAPRPVVIG YASLPLSTYI HSRSDISLPV MRELVPHYLQ ETTKERLDYL EDGKSIFKLR LRLCSSLYPT NERVRDFCLE YDRHTLRTSP PWGSELLQAI
0901: NSLKHVDSTA LLQFLYPILN MLLHLIGNGG ETLQVAAFRA MVDILTRVQQ VSFDDADRNR FLVTYVDYSF DDFGGNHPPV YPGLSTVWGS LARSKAKGYR
1001: VGPVYDDVLS MAWFFLELIV KSMALEQARL FDHNLPSGED VPPMQLKESV FRCIMQLFDC LLTEVHERCK KGLSLAKRLN SSLAFFCYDL LYIIEPCQVY
1101: ELVSLYMDKF SGVCQSILHE CKLTFLQIIS DHDLFVEMPG RDPSDRNYLS SILIQELFLS LDHDELPLRA KGARILVILL CKHEFDARYQ KAEDKLYIAQ
1201: LYFPFVGQIL DEMPVFYNLN ATEKREVLIG VLQIVRNLDD TSLVKAWQQS IARTRLYFKL MEECLILFEH KKAADSILGG NNSRGPVSEG TGSPKYSERL
1301: SPAINNYLSE ASRQEVRLEG TPDNGYLWQR VNSQLASPSQ PYSLREALAQ AQSSRIGASA QALRESLHPI LRQKLELWEE NVSATVSLQV LEITEKFSSM
1401: AASHNIATDY GKLDCITTIL TSFFSRNQSL AFWKAFFPIF NRIFDLHGAT LMARENDRFL KQIAFHLLRL AVYRNDSVRK RAVIGLQILV KSSLYFMQTA
1501: RLRALLTITL SELMSDVQVT HMKTDNTLEE SGEARRLQLS LSEMADEAKS VTLLRECGLP DDTLLVIPEK FTENRWSWAQ VKQLSDSLVL ALDASLGHAL
1601: LGSVMAMDRY AAAESFYKLG MAFAPVPDLH IMWLLHLCDA HQEMQSWAEA AQCAVAVAGV IMQALVARND GVWSKDHVSA LRKICPMVSG EFTTEASAAE
1701: VEGYGASKLT VDSAVKYLQL ANKLFSQAEL YHFCASILEL VIPVYKSRKA YGQLAKCHTL LTNIYESILD QESNPIPFID ATYYRVGFYG EKFGKLDRKE
1801: YVYREPRDVR LGDIMEKLSH IYESRMDSNH ILHIIPDSRQ VKAEELQAGA CYLQITAVDA VMEDEDLGSR RERIFSLSTG SVRARVFDRF LFDTPFTKNG
1901: KTQGGLEDQW KRRTVLQTEG SFPALVNRLL VTKSESLEFS PVENAIGMIE TRTTALRNEL EEPRSSDGDH LPRLQSLQRI LQGSVAVQVN SGVLSVCTAF
2001: LSGEPATRLR SQELQQLIAA LLEFMAVCKR AIRVHFRLIG EEDQEFHTQL VNGFQSLTAE LSHYIPAILS EL
0101: FETFARAISV FHPKARMEDK LEFAFKLYDL KHQGFIERQE LRQMMVATLA ESGMNLSDGV IEIIIDKIDK EEWQSLVLRH PSLLQNMTLQ HLNVKRIRLV
0201: LFLFSRMECN EQDWNQGYLS TNSPNVLPFP LPSSSDAVAD ISPISKPWRT IAFASARFLG SLFLSPSSNL WYLDGNLEQW PHLNQLVQCY GTEWIKDANK
0301: YGHYDTTRPD TFQTQIFEGP DTDTETEFRL ASARSATLDE DVASVSGTPF TDSGSPKHFG LPPLPAYEPA FDWENERSMI CGQRTPESPA ASYSSGLKIS
0401: VRVLSLAFQS GLVEPFYGSI SLYNQERKEK LSEDFYFHIS PTEMQDAKPS SENRGVFYLD APSASVCLLI QLEKTATEEG GVTTSVYSRK EPVHLTEREK
0501: QKLQVWSRIM PYRESFAWAV VPLFDNSITT NTGESASPSS PLAPSMTASS SHDGVFEPMA KITSDGKQGS SGGSSVVVEI SNLNKVKENY SEESIQDPKR
0601: KVHKPVKGVL RLEIEKHRNG HGDVEDLSEN GSIRNESLDL TDRLGDLTLM KCPSAGSGGP HNSGSKWSTE DVSKNLTSSS GNVDNCYYAF DFCSTTRNEP
0701: FLHLFHCLYV YPVAVTLSRK RNPFIRVELR KDDTDVRKQP LEALYPREPG VSLQKWAHTQ VAVGARAASY HDEIKVSLPA TWTPSHHLLF TFFHVDLQTK
0801: LEAPRPVVIG YASLPLSTYI HSRSDISLPV MRELVPHYLQ ETTKERLDYL EDGKSIFKLR LRLCSSLYPT NERVRDFCLE YDRHTLRTSP PWGSELLQAI
0901: NSLKHVDSTA LLQFLYPILN MLLHLIGNGG ETLQVAAFRA MVDILTRVQQ VSFDDADRNR FLVTYVDYSF DDFGGNHPPV YPGLSTVWGS LARSKAKGYR
1001: VGPVYDDVLS MAWFFLELIV KSMALEQARL FDHNLPSGED VPPMQLKESV FRCIMQLFDC LLTEVHERCK KGLSLAKRLN SSLAFFCYDL LYIIEPCQVY
1101: ELVSLYMDKF SGVCQSILHE CKLTFLQIIS DHDLFVEMPG RDPSDRNYLS SILIQELFLS LDHDELPLRA KGARILVILL CKHEFDARYQ KAEDKLYIAQ
1201: LYFPFVGQIL DEMPVFYNLN ATEKREVLIG VLQIVRNLDD TSLVKAWQQS IARTRLYFKL MEECLILFEH KKAADSILGG NNSRGPVSEG TGSPKYSERL
1301: SPAINNYLSE ASRQEVRLEG TPDNGYLWQR VNSQLASPSQ PYSLREALAQ AQSSRIGASA QALRESLHPI LRQKLELWEE NVSATVSLQV LEITEKFSSM
1401: AASHNIATDY GKLDCITTIL TSFFSRNQSL AFWKAFFPIF NRIFDLHGAT LMARENDRFL KQIAFHLLRL AVYRNDSVRK RAVIGLQILV KSSLYFMQTA
1501: RLRALLTITL SELMSDVQVT HMKTDNTLEE SGEARRLQLS LSEMADEAKS VTLLRECGLP DDTLLVIPEK FTENRWSWAQ VKQLSDSLVL ALDASLGHAL
1601: LGSVMAMDRY AAAESFYKLG MAFAPVPDLH IMWLLHLCDA HQEMQSWAEA AQCAVAVAGV IMQALVARND GVWSKDHVSA LRKICPMVSG EFTTEASAAE
1701: VEGYGASKLT VDSAVKYLQL ANKLFSQAEL YHFCASILEL VIPVYKSRKA YGQLAKCHTL LTNIYESILD QESNPIPFID ATYYRVGFYG EKFGKLDRKE
1801: YVYREPRDVR LGDIMEKLSH IYESRMDSNH ILHIIPDSRQ VKAEELQAGA CYLQITAVDA VMEDEDLGSR RERIFSLSTG SVRARVFDRF LFDTPFTKNG
1901: KTQGGLEDQW KRRTVLQTEG SFPALVNRLL VTKSESLEFS PVENAIGMIE TRTTALRNEL EEPRSSDGDH LPRLQSLQRI LQGSVAVQVN SGVLSVCTAF
2001: LSGEPATRLR SQELQQLIAA LLEFMAVCKR AIRVHFRLIG EEDQEFHTQL VNGFQSLTAE LSHYIPAILS EL
0001: MENNNLGLRF RKLPRQPLAL PKLDPLLDEN LEQWPHLNQL VQCYGTEWVK DVNKYGHYEN IRPDSFQTQI FEGPDTDTET EIRLASARSA TIEEDVASIS
0101: GRPFSDPGSS KHFGQPPLPA YEPAFDWENE RAMIFGQRTP ESPAASYSSG LKISVRVLSL AFQSGLVEPF FGSIALYNQE RKEKLSEDFY FQIQPTEMQD
0201: AKLSSENRGV FYLDAPSASV CLLIQLEKTA TEEGGVTSSV YSRKEPVHLT EREKQKLQVW SRIMPYRESF AWAVVPLFDN NLTTNTGESA SPSSPLAPSM
0301: TASSSHDGVY EPIAKITSDG KQGYSGGSSV VVEISNLNKV KESYSEESIQ DPKRKVHKPV KGVLRLEIEK HRNGHGDFED LSENGSIIND SLDPTDRLSD
0401: LTLMKCPSSS SGGPRNGCSK WNSEDAKDVS RNLTSSCGTP DLNCYHAFDF CSTTRNEPFL HLFHCLYVYP VAVTLSRKRN PFIRVELRKD DTDIRKQPLE
0501: AIYPREPGVS LQKWVHTQVA VGARAASYHD EIKVSLPATW TPSHHLLFTF FHVDLQTKLE APRPVVVGYA SLPLSTYIHS RSDISLPVMR ELVPHYLQES
0601: TKERLDYLED GKNIFKLRLR LCSSLYPTNE RVRDFCLEYD RHTLQTRPPW GSELLQAINS LKHVDSTALL QFLYPILNML LHLIGNGGET LQVAAFRAMV
0701: DILTRVQQVS FDDADRNRFL VTYVDYSFDD FGGNQPPVYP GLATVWGSLA RSKAKGYRVG PVYDDVLSMA WFFLELIVKS MALEQARLYD HNLPTGEDVP
0801: PMQLKESVFR CIMQLFDCLL TEVHERCKKG LSLAKRLNSS LAFFCYDLLY IIEPCQVYEL VSLYMDKFSG VCQSVLHECK LTFLQIISDH DLFVEMPGRD
0901: PSDRNYLSSI LIQELFLSLD HDELPLRAKG ARILVILLCK HEFDARYQKA EDKLYIAQLY FPFVGQILDE MPVFYNLNAT EKREVLIGVL QIVRNLDDTS
1001: LVKAWQQSIA RTRLYFKLME ECLILFEHKK AADSILGGNN SRGPVSEGAG SPKYSERLSP AINNYLSEAS RQEVRLEGTP DNGYLWQRVN SQLASPSQPY
1101: SLREALAQAQ SSRIGASAQA LRESLHPILR QKLELWEENV SATVSLQVLE ITENFSSMAA SHNIATDYGK LDCITTILTS FFSRNQSLAF WKAFFPIFNR
1201: IFDLHGATLM ARENDRFLKQ IAFHLLRLAV YRNDSVRKRA VIGLQILVKS SLYFMQTARL RALLTITLSE LMSDVQVTHM KSDNTLEESG EARRLQQSLS
1301: EMADEAKSVN LLRECGLPDD TLLIIPEKFT ENRWSWAEVK HLSDSLVLAL DASLGHALLG SVMAMDRYAA AESFYKLGMA FAPVPDLHIM WLLHLCDAHQ
1401: EMQSWAEAAQ CAVAVAGVIM QALVARNDGV WSKDHVSALR KICPMVSGEF TTEASAAEVE GYGASKLTVD SAVKYLQLAN KLFSQAELYH FCASILELVI
1501: PVYKSRKAYG QLAKCHTLLT NIYESILDQE SNPIPFIDAT YYRVGFYGEK FGKLDRKEYV YREPRDVRLG DIMEKLSHIY ESRMDSNHIL HIIPDSRQVK
1601: AEDLQAGVCY LQITAVDAVM EDEDLGSRRE RIFSLSTGSV RARVFDRFLF DTPFTKNGKT QGGLEDQWKR RTVLQTEGSF PALVNRLLVT KSESLEFSPV
1701: ENAIGMIETR TTALRNELEE PRSSDGDHLP RLQSLQRILQ GSVAVQVNSG VLSVCTAFLS GEPATRLRSQ ELQQLIAALL EFMAVCKRAI RVHFRLIGEE
1801: DQEFHTQLVN GFQSLTAELS HYIPAILSEL
0101: GRPFSDPGSS KHFGQPPLPA YEPAFDWENE RAMIFGQRTP ESPAASYSSG LKISVRVLSL AFQSGLVEPF FGSIALYNQE RKEKLSEDFY FQIQPTEMQD
0201: AKLSSENRGV FYLDAPSASV CLLIQLEKTA TEEGGVTSSV YSRKEPVHLT EREKQKLQVW SRIMPYRESF AWAVVPLFDN NLTTNTGESA SPSSPLAPSM
0301: TASSSHDGVY EPIAKITSDG KQGYSGGSSV VVEISNLNKV KESYSEESIQ DPKRKVHKPV KGVLRLEIEK HRNGHGDFED LSENGSIIND SLDPTDRLSD
0401: LTLMKCPSSS SGGPRNGCSK WNSEDAKDVS RNLTSSCGTP DLNCYHAFDF CSTTRNEPFL HLFHCLYVYP VAVTLSRKRN PFIRVELRKD DTDIRKQPLE
0501: AIYPREPGVS LQKWVHTQVA VGARAASYHD EIKVSLPATW TPSHHLLFTF FHVDLQTKLE APRPVVVGYA SLPLSTYIHS RSDISLPVMR ELVPHYLQES
0601: TKERLDYLED GKNIFKLRLR LCSSLYPTNE RVRDFCLEYD RHTLQTRPPW GSELLQAINS LKHVDSTALL QFLYPILNML LHLIGNGGET LQVAAFRAMV
0701: DILTRVQQVS FDDADRNRFL VTYVDYSFDD FGGNQPPVYP GLATVWGSLA RSKAKGYRVG PVYDDVLSMA WFFLELIVKS MALEQARLYD HNLPTGEDVP
0801: PMQLKESVFR CIMQLFDCLL TEVHERCKKG LSLAKRLNSS LAFFCYDLLY IIEPCQVYEL VSLYMDKFSG VCQSVLHECK LTFLQIISDH DLFVEMPGRD
0901: PSDRNYLSSI LIQELFLSLD HDELPLRAKG ARILVILLCK HEFDARYQKA EDKLYIAQLY FPFVGQILDE MPVFYNLNAT EKREVLIGVL QIVRNLDDTS
1001: LVKAWQQSIA RTRLYFKLME ECLILFEHKK AADSILGGNN SRGPVSEGAG SPKYSERLSP AINNYLSEAS RQEVRLEGTP DNGYLWQRVN SQLASPSQPY
1101: SLREALAQAQ SSRIGASAQA LRESLHPILR QKLELWEENV SATVSLQVLE ITENFSSMAA SHNIATDYGK LDCITTILTS FFSRNQSLAF WKAFFPIFNR
1201: IFDLHGATLM ARENDRFLKQ IAFHLLRLAV YRNDSVRKRA VIGLQILVKS SLYFMQTARL RALLTITLSE LMSDVQVTHM KSDNTLEESG EARRLQQSLS
1301: EMADEAKSVN LLRECGLPDD TLLIIPEKFT ENRWSWAEVK HLSDSLVLAL DASLGHALLG SVMAMDRYAA AESFYKLGMA FAPVPDLHIM WLLHLCDAHQ
1401: EMQSWAEAAQ CAVAVAGVIM QALVARNDGV WSKDHVSALR KICPMVSGEF TTEASAAEVE GYGASKLTVD SAVKYLQLAN KLFSQAELYH FCASILELVI
1501: PVYKSRKAYG QLAKCHTLLT NIYESILDQE SNPIPFIDAT YYRVGFYGEK FGKLDRKEYV YREPRDVRLG DIMEKLSHIY ESRMDSNHIL HIIPDSRQVK
1601: AEDLQAGVCY LQITAVDAVM EDEDLGSRRE RIFSLSTGSV RARVFDRFLF DTPFTKNGKT QGGLEDQWKR RTVLQTEGSF PALVNRLLVT KSESLEFSPV
1701: ENAIGMIETR TTALRNELEE PRSSDGDHLP RLQSLQRILQ GSVAVQVNSG VLSVCTAFLS GEPATRLRSQ ELQQLIAALL EFMAVCKRAI RVHFRLIGEE
1801: DQEFHTQLVN GFQSLTAELS HYIPAILSEL
Arabidopsis Description
SPK1Guanine nucleotide exchange factor SPIKE 1 [Source:UniProtKB/Swiss-Prot;Acc:Q8SAB7]
SUBAcon: [cytosol]
SUBAcon: [cytosol]
Hydropathy Plot
About CropPAL
The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.