Subcellular Localization
min:
: max
Winner_takes_all: cytosol
Predictor Summary:
Predictor Summary:
- cytosol 2
- mitochondrion 1
- plasma membrane 1
| Predictors | GFP | MS/MS | Papers | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
PPI
Inferred distinct locusB in Crop
Inferred from Arabidopsis experimental PPI
Homology
Paralog
| locus | Identity | Homology Identity |
|---|
Ortholog
| locus | Homology Species | Location | Identity | Homology Identity |
|---|---|---|---|---|
| Os03t0328000-01 | Rice | plasma membrane | 14.85 | 98.58 |
| KXG39465 | Sorghum | cytosol | 95.5 | 96.85 |
| Zm00001d047540_P001 | Maize | cytosol, mitochondrion | 94.58 | 95.56 |
| HORVU4Hr1G051250.3 | Barley | cytosol | 89.81 | 91.33 |
| TraesCS4B01G173500.4 | Wheat | cytosol | 89.49 | 90.95 |
| TraesCS4D01G175600.4 | Wheat | cytosol | 89.44 | 90.9 |
| TraesCS4A01G131100.1 | Wheat | cytosol | 89.44 | 90.9 |
| Zm00001d012342_P001 | Maize | cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole | 11.26 | 90.52 |
| GSMUA_Achr10P... | Banana | extracellular | 50.35 | 88.5 |
| Zm00001d024153_P001 | Maize | cytosol | 7.02 | 86.18 |
| Solyc08g016060.1.1 | Tomato | extracellular | 12.65 | 82.81 |
| Solyc08g016050.2.1 | Tomato | nucleus | 39.79 | 81.9 |
| Zm00001d035539_P001 | Maize | cytosol | 8.79 | 80.0 |
| VIT_11s0016g00100.t01 | Wine grape | mitochondrion | 78.12 | 78.88 |
| KRH43411 | Soybean | endoplasmic reticulum | 75.6 | 76.84 |
| KRH13046 | Soybean | endoplasmic reticulum | 75.39 | 76.62 |
| AT4G16340.1 | Thale cress | cytosol | 74.05 | 75.46 |
| CDY32235 | Canola | cytosol | 73.46 | 75.11 |
| GSMUA_Achr10P... | Banana | cytosol | 31.42 | 72.52 |
| CDY34451 | Canola | endoplasmic reticulum | 72.49 | 71.61 |
| Bra038448.1-P | Field mustard | cytosol | 73.03 | 65.73 |
| Solyc08g016070.1.1 | Tomato | mitochondrion | 20.86 | 63.35 |
| Zm00001d042645_P001 | Maize | cytosol, nucleus, peroxisome | 18.77 | 59.63 |
| Zm00001d007384_P003 | Maize | cytosol, endoplasmic reticulum, peroxisome | 7.51 | 39.11 |
| Zm00001d035534_P003 | Maize | cytosol | 8.2 | 32.35 |
| Zm00001d046856_P002 | Maize | endoplasmic reticulum, nucleus, peroxisome | 8.9 | 31.14 |
| Zm00001d038664_P001 | Maize | cytosol, nucleus, plastid | 8.2 | 28.6 |
Protein Annotations
| Gene3D:1.20.58.740 | Gene3D:1.25.40.410 | EntrezGene:100273928 | MapMan:27.4.3.2 | UniProt:A0A1D6K1C7 | InterPro:ARM-type_fold |
| ncoils:Coil | InterPro:DHR-1_domain | InterPro:DHR-2 | InterPro:DOCK | InterPro:DOCK_C | GO:GO:0003674 |
| GO:GO:0005085 | GO:GO:0005488 | GO:GO:0005515 | GO:GO:0005575 | GO:GO:0005622 | GO:GO:0005623 |
| GO:GO:0007154 | GO:GO:0007165 | GO:GO:0007264 | GO:GO:0008150 | GO:GO:0009987 | GO:GO:0065009 |
| InterPro:IPR027007 | InterPro:IPR027357 | ProteinID:ONL97587.1 | PFAM:PF06920 | PFAM:PF14429 | PFscan:PS51650 |
| PFscan:PS51651 | PANTHER:PTHR23317 | PANTHER:PTHR23317:SF76 | SUPFAM:SSF48371 | UniParc:UPI000845610F | EnsemblPlantsGene:Zm00001d029004 |
| EnsemblPlants:Zm00001d029004_P004 | EnsemblPlants:Zm00001d029004_T004 | SEG:seg | : | : | : |
Description
Guanine nucleotide exchange factor SPIKE 1
Coordinates
chr1:+:54451966..54480478
Molecular Weight (calculated)
210249.0 Da
IEP (calculated)
6.274
GRAVY (calculated)
-0.201
Length
1865 amino acids
Sequence
(BLAST)
(BLAST)
0001: MEPAVATAGE GQRFKRIPRQ AWSGNLELGP LLNESLDHWP HLNELVQCYK ADFVKDDCKY GHYESVASPS FQNQIFEGPD TDIETELQLC NARHSKSEDA
0101: TEDDTPSTSG RQIYETESSG SSSKVHCSLS PLPAYEAAFD WENERSLIFG QRVPESIPAI SNSGLKITVK VLSLSFQAGL VEPFSGTICL YNRDRRERLS
0201: EDFYFHMLPT DMQDQGSLDR RGVFSLDAPS PSVCLLIQLE KAATEEGGVT PSVYSRKEPV HLAEKEKQKL QVWSRIMPYK ESFAWAMIPL FESNRAGGLS
0301: DAASPSSPLA PSLSGSSSQD SIVDPISKLA LDGKVNHYSS GSSVIVEISN LNKVKESYIE DSLQDPKRKV HKPVKGVLRL EVEKLHDDHN DVDNASEGGS
0401: MANDLNDAGD INNGRSNRSS FDGIHSSVNS IAIAKKDAHH NGHTSNAENV DNFQAFDFRV LTRSEPFSQL FHCLYVYPLT VSLSRKRNLF VRVELRKDDS
0501: DIRKPPLEAV HPRERNTMLQ KWGHTQIAVG TRMASYHDEV KISLPALLTP QHHLVFTFFH VDLQMKLEAP KPVIIGYSVL PLSTHIQYAS STQLFFSVLV
0601: TSYLLFVTCQ LLQMLLSDVS LPILRELVPH YLQESGKERM DYLEDGKTVF RLRLRLCSSL FPINERIRDF FVEYDRHTLH TSPPWGSELL EAINSLKNVE
0701: STALLQFLQP ILNMLLHLIG DGGETLQVAA FRAMVNILTR VQQESSDGAE RNRFLINYVD FAFDDFGDRQ APVYPGLTTV WGSLARSKAK GYRVGPVYDD
0801: VLAMAWFFLE LIVKSMGLEQ SRLFYHNLPL GEDVPPLQLK EGVFRCIMQL FDCLLTEVHE RCKKGLSLAK RLNSTLAFFC YDLLSIIEPR QVFELVSLYM
0901: DKFAGVCQSV LHDCKLTFLQ IICDHDLFVE MPGRDPSDRN YLSSVLIQEI FLTLDHDDLS QRAKAARILV VLICKHEFDA RYQKSEDKLY IAQLYFPLIG
1001: QILDEMPVFY NLNAIEKREV LVVILQIVRN LDDATLIKAW QQSIARTRLF FKLLEECIIH FEHNKTGDNM LLGASSRSPD VEHPASPKYS ERLSPSVNTY
1101: LSEASRHEIR PQGTPENGYM WNRVSPQLSS PNQPYSLREA LAQAQSSRIG STARALRESL HPVLRQKMEL WEENLSTAVS LEVLGITEKF SVAAGARSIT
1201: TDYAKLDCVT SILMGLLSRN QSLAFWKAFL PVVYNIFNVH GATLMAREND RFLKQIAFHL LRLAVFRNDS IRKRAVVGLQ ILVRNSFNYF KNTTRLRVML
1301: TITLSELMSD VQVTQMKFDG SLEESGEARR LRKSLEEMAD VRSKDLLKDC GLPVTALEAA PEGSSDNRWS WVEVKHLSKC LVQALDAGLE HALLGSVVTV
1401: DRYAAAEGFY KLAMAYAPVP DLHIMWLLHL CDAHQEMQSW AEAAQCAVAV AGVIMQALVG RNDAVWSKEH VASLRKICPI VNTDVSAEAS AAEVEGYGAS
1501: KLTVDSAVKY LQLANKLFTQ AELYHFCASI QELIIPVYKS RRAYGQLAKC HTSLTNIYES ILEQEASPIP FIDATYYRVG FYGERFGKLN KKEYVFREPR
1601: DVRLGDIMEK LSHIYEAKID GSHTLHIIPD SRQVNADELQ PGFCYLQITA VDPVMEDEDL GSRRERIFSL STGTVRARVF DRFLFDTPFT KNGKTQGGLE
1701: DQWKRRTVLQ TEGSFPALVN RLPVIKSESL EFSPVENAIG MIETRTAALR NELEEPRSSE GDQLPRLQSL QRILQGSVAV QVNSGVLSVC TAFLSGEPAT
1801: RLRSQELQQL IAALLEFMAV CKRAIRVHFR LIGEEDQEFH TQLVNGFQSL TAELSHYIPA ILSEL
0101: TEDDTPSTSG RQIYETESSG SSSKVHCSLS PLPAYEAAFD WENERSLIFG QRVPESIPAI SNSGLKITVK VLSLSFQAGL VEPFSGTICL YNRDRRERLS
0201: EDFYFHMLPT DMQDQGSLDR RGVFSLDAPS PSVCLLIQLE KAATEEGGVT PSVYSRKEPV HLAEKEKQKL QVWSRIMPYK ESFAWAMIPL FESNRAGGLS
0301: DAASPSSPLA PSLSGSSSQD SIVDPISKLA LDGKVNHYSS GSSVIVEISN LNKVKESYIE DSLQDPKRKV HKPVKGVLRL EVEKLHDDHN DVDNASEGGS
0401: MANDLNDAGD INNGRSNRSS FDGIHSSVNS IAIAKKDAHH NGHTSNAENV DNFQAFDFRV LTRSEPFSQL FHCLYVYPLT VSLSRKRNLF VRVELRKDDS
0501: DIRKPPLEAV HPRERNTMLQ KWGHTQIAVG TRMASYHDEV KISLPALLTP QHHLVFTFFH VDLQMKLEAP KPVIIGYSVL PLSTHIQYAS STQLFFSVLV
0601: TSYLLFVTCQ LLQMLLSDVS LPILRELVPH YLQESGKERM DYLEDGKTVF RLRLRLCSSL FPINERIRDF FVEYDRHTLH TSPPWGSELL EAINSLKNVE
0701: STALLQFLQP ILNMLLHLIG DGGETLQVAA FRAMVNILTR VQQESSDGAE RNRFLINYVD FAFDDFGDRQ APVYPGLTTV WGSLARSKAK GYRVGPVYDD
0801: VLAMAWFFLE LIVKSMGLEQ SRLFYHNLPL GEDVPPLQLK EGVFRCIMQL FDCLLTEVHE RCKKGLSLAK RLNSTLAFFC YDLLSIIEPR QVFELVSLYM
0901: DKFAGVCQSV LHDCKLTFLQ IICDHDLFVE MPGRDPSDRN YLSSVLIQEI FLTLDHDDLS QRAKAARILV VLICKHEFDA RYQKSEDKLY IAQLYFPLIG
1001: QILDEMPVFY NLNAIEKREV LVVILQIVRN LDDATLIKAW QQSIARTRLF FKLLEECIIH FEHNKTGDNM LLGASSRSPD VEHPASPKYS ERLSPSVNTY
1101: LSEASRHEIR PQGTPENGYM WNRVSPQLSS PNQPYSLREA LAQAQSSRIG STARALRESL HPVLRQKMEL WEENLSTAVS LEVLGITEKF SVAAGARSIT
1201: TDYAKLDCVT SILMGLLSRN QSLAFWKAFL PVVYNIFNVH GATLMAREND RFLKQIAFHL LRLAVFRNDS IRKRAVVGLQ ILVRNSFNYF KNTTRLRVML
1301: TITLSELMSD VQVTQMKFDG SLEESGEARR LRKSLEEMAD VRSKDLLKDC GLPVTALEAA PEGSSDNRWS WVEVKHLSKC LVQALDAGLE HALLGSVVTV
1401: DRYAAAEGFY KLAMAYAPVP DLHIMWLLHL CDAHQEMQSW AEAAQCAVAV AGVIMQALVG RNDAVWSKEH VASLRKICPI VNTDVSAEAS AAEVEGYGAS
1501: KLTVDSAVKY LQLANKLFTQ AELYHFCASI QELIIPVYKS RRAYGQLAKC HTSLTNIYES ILEQEASPIP FIDATYYRVG FYGERFGKLN KKEYVFREPR
1601: DVRLGDIMEK LSHIYEAKID GSHTLHIIPD SRQVNADELQ PGFCYLQITA VDPVMEDEDL GSRRERIFSL STGTVRARVF DRFLFDTPFT KNGKTQGGLE
1701: DQWKRRTVLQ TEGSFPALVN RLPVIKSESL EFSPVENAIG MIETRTAALR NELEEPRSSE GDQLPRLQSL QRILQGSVAV QVNSGVLSVC TAFLSGEPAT
1801: RLRSQELQQL IAALLEFMAV CKRAIRVHFR LIGEEDQEFH TQLVNGFQSL TAELSHYIPA ILSEL
0001: MENNNLGLRF RKLPRQPLAL PKLDPLLDEN LEQWPHLNQL VQCYGTEWVK DVNKYGHYEN IRPDSFQTQI FEGPDTDTET EIRLASARSA TIEEDVASIS
0101: GRPFSDPGSS KHFGQPPLPA YEPAFDWENE RAMIFGQRTP ESPAASYSSG LKISVRVLSL AFQSGLVEPF FGSIALYNQE RKEKLSEDFY FQIQPTEMQD
0201: AKLSSENRGV FYLDAPSASV CLLIQLEKTA TEEGGVTSSV YSRKEPVHLT EREKQKLQVW SRIMPYRESF AWAVVPLFDN NLTTNTGESA SPSSPLAPSM
0301: TASSSHDGVY EPIAKITSDG KQGYSGGSSV VVEISNLNKV KESYSEESIQ DPKRKVHKPV KGVLRLEIEK HRNGHGDFED LSENGSIIND SLDPTDRLSD
0401: LTLMKCPSSS SGGPRNGCSK WNSEDAKDVS RNLTSSCGTP DLNCYHAFDF CSTTRNEPFL HLFHCLYVYP VAVTLSRKRN PFIRVELRKD DTDIRKQPLE
0501: AIYPREPGVS LQKWVHTQVA VGARAASYHD EIKVSLPATW TPSHHLLFTF FHVDLQTKLE APRPVVVGYA SLPLSTYIHS RSDISLPVMR ELVPHYLQES
0601: TKERLDYLED GKNIFKLRLR LCSSLYPTNE RVRDFCLEYD RHTLQTRPPW GSELLQAINS LKHVDSTALL QFLYPILNML LHLIGNGGET LQVAAFRAMV
0701: DILTRVQQVS FDDADRNRFL VTYVDYSFDD FGGNQPPVYP GLATVWGSLA RSKAKGYRVG PVYDDVLSMA WFFLELIVKS MALEQARLYD HNLPTGEDVP
0801: PMQLKESVFR CIMQLFDCLL TEVHERCKKG LSLAKRLNSS LAFFCYDLLY IIEPCQVYEL VSLYMDKFSG VCQSVLHECK LTFLQIISDH DLFVEMPGRD
0901: PSDRNYLSSI LIQELFLSLD HDELPLRAKG ARILVILLCK HEFDARYQKA EDKLYIAQLY FPFVGQILDE MPVFYNLNAT EKREVLIGVL QIVRNLDDTS
1001: LVKAWQQSIA RTRLYFKLME ECLILFEHKK AADSILGGNN SRGPVSEGAG SPKYSERLSP AINNYLSEAS RQEVRLEGTP DNGYLWQRVN SQLASPSQPY
1101: SLREALAQAQ SSRIGASAQA LRESLHPILR QKLELWEENV SATVSLQVLE ITENFSSMAA SHNIATDYGK LDCITTILTS FFSRNQSLAF WKAFFPIFNR
1201: IFDLHGATLM ARENDRFLKQ IAFHLLRLAV YRNDSVRKRA VIGLQILVKS SLYFMQTARL RALLTITLSE LMSDVQVTHM KSDNTLEESG EARRLQQSLS
1301: EMADEAKSVN LLRECGLPDD TLLIIPEKFT ENRWSWAEVK HLSDSLVLAL DASLGHALLG SVMAMDRYAA AESFYKLGMA FAPVPDLHIM WLLHLCDAHQ
1401: EMQSWAEAAQ CAVAVAGVIM QALVARNDGV WSKDHVSALR KICPMVSGEF TTEASAAEVE GYGASKLTVD SAVKYLQLAN KLFSQAELYH FCASILELVI
1501: PVYKSRKAYG QLAKCHTLLT NIYESILDQE SNPIPFIDAT YYRVGFYGEK FGKLDRKEYV YREPRDVRLG DIMEKLSHIY ESRMDSNHIL HIIPDSRQVK
1601: AEDLQAGVCY LQITAVDAVM EDEDLGSRRE RIFSLSTGSV RARVFDRFLF DTPFTKNGKT QGGLEDQWKR RTVLQTEGSF PALVNRLLVT KSESLEFSPV
1701: ENAIGMIETR TTALRNELEE PRSSDGDHLP RLQSLQRILQ GSVAVQVNSG VLSVCTAFLS GEPATRLRSQ ELQQLIAALL EFMAVCKRAI RVHFRLIGEE
1801: DQEFHTQLVN GFQSLTAELS HYIPAILSEL
0101: GRPFSDPGSS KHFGQPPLPA YEPAFDWENE RAMIFGQRTP ESPAASYSSG LKISVRVLSL AFQSGLVEPF FGSIALYNQE RKEKLSEDFY FQIQPTEMQD
0201: AKLSSENRGV FYLDAPSASV CLLIQLEKTA TEEGGVTSSV YSRKEPVHLT EREKQKLQVW SRIMPYRESF AWAVVPLFDN NLTTNTGESA SPSSPLAPSM
0301: TASSSHDGVY EPIAKITSDG KQGYSGGSSV VVEISNLNKV KESYSEESIQ DPKRKVHKPV KGVLRLEIEK HRNGHGDFED LSENGSIIND SLDPTDRLSD
0401: LTLMKCPSSS SGGPRNGCSK WNSEDAKDVS RNLTSSCGTP DLNCYHAFDF CSTTRNEPFL HLFHCLYVYP VAVTLSRKRN PFIRVELRKD DTDIRKQPLE
0501: AIYPREPGVS LQKWVHTQVA VGARAASYHD EIKVSLPATW TPSHHLLFTF FHVDLQTKLE APRPVVVGYA SLPLSTYIHS RSDISLPVMR ELVPHYLQES
0601: TKERLDYLED GKNIFKLRLR LCSSLYPTNE RVRDFCLEYD RHTLQTRPPW GSELLQAINS LKHVDSTALL QFLYPILNML LHLIGNGGET LQVAAFRAMV
0701: DILTRVQQVS FDDADRNRFL VTYVDYSFDD FGGNQPPVYP GLATVWGSLA RSKAKGYRVG PVYDDVLSMA WFFLELIVKS MALEQARLYD HNLPTGEDVP
0801: PMQLKESVFR CIMQLFDCLL TEVHERCKKG LSLAKRLNSS LAFFCYDLLY IIEPCQVYEL VSLYMDKFSG VCQSVLHECK LTFLQIISDH DLFVEMPGRD
0901: PSDRNYLSSI LIQELFLSLD HDELPLRAKG ARILVILLCK HEFDARYQKA EDKLYIAQLY FPFVGQILDE MPVFYNLNAT EKREVLIGVL QIVRNLDDTS
1001: LVKAWQQSIA RTRLYFKLME ECLILFEHKK AADSILGGNN SRGPVSEGAG SPKYSERLSP AINNYLSEAS RQEVRLEGTP DNGYLWQRVN SQLASPSQPY
1101: SLREALAQAQ SSRIGASAQA LRESLHPILR QKLELWEENV SATVSLQVLE ITENFSSMAA SHNIATDYGK LDCITTILTS FFSRNQSLAF WKAFFPIFNR
1201: IFDLHGATLM ARENDRFLKQ IAFHLLRLAV YRNDSVRKRA VIGLQILVKS SLYFMQTARL RALLTITLSE LMSDVQVTHM KSDNTLEESG EARRLQQSLS
1301: EMADEAKSVN LLRECGLPDD TLLIIPEKFT ENRWSWAEVK HLSDSLVLAL DASLGHALLG SVMAMDRYAA AESFYKLGMA FAPVPDLHIM WLLHLCDAHQ
1401: EMQSWAEAAQ CAVAVAGVIM QALVARNDGV WSKDHVSALR KICPMVSGEF TTEASAAEVE GYGASKLTVD SAVKYLQLAN KLFSQAELYH FCASILELVI
1501: PVYKSRKAYG QLAKCHTLLT NIYESILDQE SNPIPFIDAT YYRVGFYGEK FGKLDRKEYV YREPRDVRLG DIMEKLSHIY ESRMDSNHIL HIIPDSRQVK
1601: AEDLQAGVCY LQITAVDAVM EDEDLGSRRE RIFSLSTGSV RARVFDRFLF DTPFTKNGKT QGGLEDQWKR RTVLQTEGSF PALVNRLLVT KSESLEFSPV
1701: ENAIGMIETR TTALRNELEE PRSSDGDHLP RLQSLQRILQ GSVAVQVNSG VLSVCTAFLS GEPATRLRSQ ELQQLIAALL EFMAVCKRAI RVHFRLIGEE
1801: DQEFHTQLVN GFQSLTAELS HYIPAILSEL
Arabidopsis Description
SPK1Guanine nucleotide exchange factor SPIKE 1 [Source:UniProtKB/Swiss-Prot;Acc:Q8SAB7]
SUBAcon: [cytosol]
SUBAcon: [cytosol]
Hydropathy Plot
About CropPAL
The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.