CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 1
cytosol 2
plastid 2
mitochondrion 3

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 27224218

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH00374
KRH03338
KRH13046
KRH40820
KRH42863
KRH43411
KRH51687
KRH53518
KRH54429
KRH54430
KRH57035
KRH61024
KRH61026
KRH63489
KRH64414
KRH65511
KRH65551
KRH65850
KRH76068

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G16340.1	KRH51687	AT1G20090.1	17267444
AT4G16340.1	KRH61024	AT1G20090.1	17267444
AT4G16340.1	KRH61026	AT1G20090.1	17267444
AT4G16340.1	KRH42863	AT1G29170.1	17267444
AT4G16340.1	KRH00374	AT2G38440.1	17267444
AT4G16340.1	KRH40820	AT2G38440.1	17267444
AT4G16340.1	KRH65511	AT2G38440.1	17267444
AT4G16340.1	KRH76068	AT2G38440.1	17267444
AT4G16340.1	KRH53518	AT3G49290.1	17267444
AT4G16340.1	KRH64414	AT3G49290.1	17267444
AT4G16340.1	KRH65551	AT3G49290.1	17267444
AT4G16340.1	KRH65850	AT3G49290.1	17267444
AT4G16340.1	KRH13046	AT4G16340.1	17267444
AT4G16340.1	KRH43411	AT4G16340.1	17267444
AT4G16340.1	KRH53518	AT5G24310.1	17267444
AT4G16340.1	KRH64414	AT5G24310.1	17267444
AT4G16340.1	KRH65551	AT5G24310.1	17267444
AT4G16340.1	KRH65850	AT5G24310.1	17267444
AT4G16340.1	KRH03338	AT5G62880.1	17267444
AT4G16340.1	KRH54429	AT5G62880.1	17267444
AT4G16340.1	KRH54430	AT5G62880.1	17267444
AT4G16340.1	KRH57035	AT5G62880.1	17267444
AT4G16340.1	KRH63489	AT5G62880.1	17267444

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH43411	Soybean	endoplasmic reticulum	97.71	97.71
Os03t0328000-01	Rice	plasma membrane	14.77	96.44
Solyc08g016050.2.1	Tomato	nucleus	42.78	86.64
GSMUA_Achr10P...	Banana	extracellular	50.08	86.62
VIT_11s0016g00100.t01	Wine grape	mitochondrion	86.43	85.87
Solyc08g016060.1.1	Tomato	extracellular	13.19	84.91
AT4G16340.1	Thale cress	cytosol	79.24	79.45
CDY32235	Canola	cytosol	78.86	79.33
KXG39465	Sorghum	cytosol	76.78	76.62
HORVU4Hr1G051250.3	Barley	cytosol	75.97	76.01
CDY34451	Canola	endoplasmic reticulum	78.09	75.9
TraesCS4B01G173500.4	Wheat	cytosol	75.59	75.59
TraesCS4D01G175600.4	Wheat	cytosol	75.48	75.48
TraesCS4A01G131100.1	Wheat	cytosol	75.48	75.48
Zm00001d047540_P001	Maize	cytosol, mitochondrion	75.86	75.41
Zm00001d029004_P004	Maize	cytosol	76.62	75.39
Bra038448.1-P	Field mustard	cytosol	78.8	69.79
Solyc08g016070.1.1	Tomato	mitochondrion	22.72	67.92
GSMUA_Achr10P...	Banana	cytosol	28.77	65.35
Zm00001d024153_P001	Maize	cytosol	4.96	59.87
Zm00001d012342_P001	Maize	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	6.59	52.16
Zm00001d035539_P001	Maize	cytosol	5.5	49.27
Zm00001d042645_P001	Maize	cytosol, nucleus, peroxisome	12.81	40.03
TraesCS5D01G127700.1	Wheat	cytosol	2.78	35.66
TraesCS5A01G195400.1	Wheat	cytosol	2.45	33.09
Zm00001d007384_P003	Maize	cytosol, endoplasmic reticulum, peroxisome	6.05	31.01
Zm00001d035534_P003	Maize	cytosol	7.08	27.48
Zm00001d046856_P002	Maize	endoplasmic reticulum, nucleus, peroxisome	7.68	26.45
Zm00001d038664_P001	Maize	cytosol, nucleus, plastid	6.7	22.99

Protein Annotations

Gene3D:1.20.58.740	Gene3D:1.25.40.410	EntrezGene:100812785	MapMan:27.4.3.2	EMBL:ACUP02009529	InterPro:ARM-type_fold
ncoils:Coil	InterPro:DHR-1_domain	InterPro:DHR-2	InterPro:DOCK	InterPro:DOCK_C	EnsemblPlantsGene:GLYMA_15G212800
GO:GO:0003674	GO:GO:0005085	GO:GO:0005488	GO:GO:0005515	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0007154	GO:GO:0007165	GO:GO:0007264	GO:GO:0008150	GO:GO:0009987
GO:GO:0065009	InterPro:IPR027007	InterPro:IPR027357	UniProt:K7MCT0	EnsemblPlants:KRH13046	ProteinID:KRH13046
ProteinID:KRH13046.1	PFAM:PF06920	PFAM:PF14429	PFscan:PS51650	PFscan:PS51651	PANTHER:PTHR23317
PANTHER:PTHR23317:SF76	SUPFAM:SSF48371	UniParc:UPI000233B428	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr15:+:33408140..33467420

Molecular Weight (calculated)

207824.0 Da

IEP (calculated)

6.135

GRAVY (calculated)

-0.226

Length

1835 amino acids

Sequence
(BLAST)

0001: MLHLRQRRDA VAPATTRWRN TFEENLEQWP HLNELVHCYT TDWVKDENKY GHYDSVGTPS FHNQIYEGPD TDIETEMRLA GARQTKGDDI SEDDIPSTSG
0101: RQFMEGADGD LLPSDVPKHI GQSLLPAYEP AFDWENERAL IFGQRIPETP VLHGMKISVK VQSLQFQAGL AEPFYGTMCL YNRERREKLS EDFYFHVLPT
0201: EMQNAKITCE PRAVFYLDAP SASVCLLIQL EKHATEEGGV TASVYSRKDP VHLTEREKQK LQVWSKIMPY KESFTWTIVS LFDSSIGAAS VGPASPSSPL
0301: APSISGSSSH EGVFDTSAKI SLDGKLSYSN GNSVVVEVSN LNKVKESYTE ESLQDPKRKM HKPIKGVLRL EIEKHQISLA DLENVSESGS ITNDSVDPGD
0401: RIVDSLSGKY PSNGCDDPQG SNLRVVSPVL GNGANQHGNS DFNADDFHAF DFRTTTRNEP FLQLFHCLYV YPLTVSLGRK RNLFIRVELR EDDGDIRRQP
0501: LEAIYPRDPG LDASFQKWGH TQVAVGARVA CYHDEIKLSL PAMWTPMHHL LFTLFHVDLQ TKLDAPKPVV IGYAALPLSS HAQLRSEINL PIMRELVPHY
0601: LQDAGRERLD YLEDGKSVFR LRLRLCSSLY PINERIRDFF LEYDRHTLRT SPPWGSELLE AINSLKNVDS TALLQFLHPI LNMLLHLIGN GGETLQVAAF
0701: RAMVNIVTRV QQESVDDAER NHFLVNYVDC AFDDFGGRQP PVYPGLSTVW GSLARSKAKG YRVGPVYDDV LAMAWFFLEL IVKSMALEKT RLFYHSLPIG
0801: EDIPPMQLKD GVFRCILQLY DCLLTEVHER CKKGLSLAKR LNSSLAFFCY DLLSIIEPRQ VFELVSLYLD KFSGVCQSVL HECKLTFLQI ICDHDLFVEM
0901: PGRDPSDRNY LSSVLIQELF VTWDHEDLSL RAKAARILVV LLCKHEFDVR YQKPEDKLYI AQLYFPLVGQ ILDEMPVFYN LNSVEKREVS IVILQIVRNL
1001: DDASLVKAWQ QSIARTRLFF KLMEECLLLF EHKKPADGML LGSSSRNPVG EAPASPKYSD RLSPAINNYL SEASRQEVRP QGTPDNGYLW QRVNSQLSSP
1101: SQPYSLREAL AQAQSSRIGA SAQALRESLH PLLRQKLELW EENLSAFISL QVLEVTEKFS MMAASHSIAT DYGKLDCITA VFMSFLSRNQ PLTFWKAFFP
1201: VFNSVFDLHG ATLMARENDR FLKQVTFHLL RLAVFQNENI RQRAVVGLQI LVRSSFHYFM QTARLRVMLI ITLSELMSDV QVTQMRSDGS LEESGEARRL
1301: RKSVDEMKDE TKNAYLLKEC GLPENALVTV PEKMTENRWS WSEVKYLSDS LLLALDGSLE HALLAPMMTM DRYAAAESFY KLAMAFAPVP DLHIMWLLHL
1401: CDAHQEMQSW AEAAQCAVAV AGVVMQALVA RNDGVWSKDH VSALRKICPM VSNEITSEAS AAEVEGYGAS KLTVDSAVKY LQLANKLFSQ AELFHFCASI
1501: LELVIPVYKS RRAYGQLAKC HTLLTNIYES ILEQESSPIP FTNATYYRVG FYGVRFGKLD KKEYVYREPR DVRLGDIMEK LSHTYESRMD GNHTLHIIPD
1601: SRQVKAEELQ PGVCYLQITA VDPVMEDEDL GSRRERIFSL STGSVRARVF DRFLFDTPFT KNGKTQGGLE DQWKRRTVLR TEGSFPALVN RLLVIKSESL
1701: EFSPVENAIG MIETRTAALR NELEEPRSSE GDQLPRLQSL QRILQGSVAV QVNSGVLSVC TAFLSGEPAT RLRSQELQQL IAALLEFMAV CKRAIRVHFR
1801: LIGEEDQDFH TQLVNGFQSL TAELSHYIPA ILSEL

Best Arabidopsis Sequence Match ( AT4G16340.2 )
(BLAST)

0001: MENNNLGLRF RKLPRQPLAL PKLDPLLDEN LEQWPHLNQL VQCYGTEWVK DVNKYGHYEN IRPDSFQTQI FEGPDTDTET EIRLASARSA TIEEDVASIS
0101: GRPFSDPGSS KHFGQPPLPA YEPAFDWENE RAMIFGQRTP ESPAASYSSG LKISVRVLSL AFQSGLVEPF FGSIALYNQE RKEKLSEDFY FQIQPTEMQD
0201: AKLSSENRGV FYLDAPSASV CLLIQLEKTA TEEGGVTSSV YSRKEPVHLT EREKQKLQVW SRIMPYRESF AWAVVPLFDN NLTTNTGESA SPSSPLAPSM
0301: TASSSHDGVY EPIAKITSDG KQGYSGGSSV VVEISNLNKV KESYSEESIQ DPKRKVHKPV KGVLRLEIEK HRNGHGDFED LSENGSIIND SLDPTDRLSD
0401: LTLMKCPSSS SGGPRNGCSK WNSEDAKDVS RNLTSSCGTP DLNCYHAFDF CSTTRNEPFL HLFHCLYVYP VAVTLSRKRN PFIRVELRKD DTDIRKQPLE
0501: AIYPREPGVS LQKWVHTQVA VGARAASYHD EIKVSLPATW TPSHHLLFTF FHVDLQTKLE APRPVVVGYA SLPLSTYIHS RSDISLPVMR ELVPHYLQES
0601: TKERLDYLED GKNIFKLRLR LCSSLYPTNE RVRDFCLEYD RHTLQTRPPW GSELLQAINS LKHVDSTALL QFLYPILNML LHLIGNGGET LQVAAFRAMV
0701: DILTRVQQVS FDDADRNRFL VTYVDYSFDD FGGNQPPVYP GLATVWGSLA RSKAKGYRVG PVYDDVLSMA WFFLELIVKS MALEQARLYD HNLPTGEDVP
0801: PMQLKESVFR CIMQLFDCLL TEVHERCKKG LSLAKRLNSS LAFFCYDLLY IIEPCQVYEL VSLYMDKFSG VCQSVLHECK LTFLQIISDH DLFVEMPGRD
0901: PSDRNYLSSI LIQELFLSLD HDELPLRAKG ARILVILLCK HEFDARYQKA EDKLYIAQLY FPFVGQILDE MPVFYNLNAT EKREVLIGVL QIVRNLDDTS
1001: LVKAWQQSIA RTRLYFKLME ECLILFEHKK AADSILGGNN SRGPVSEGAG SPKYSERLSP AINNYLSEAS RQEVRLEGTP DNGYLWQRVN SQLASPSQPY
1101: SLREALAQAQ SSRIGASAQA LRESLHPILR QKLELWEENV SATVSLQVLE ITENFSSMAA SHNIATDYGK LDCITTILTS FFSRNQSLAF WKAFFPIFNR
1201: IFDLHGATLM ARENDRFLKQ IAFHLLRLAV YRNDSVRKRA VIGLQILVKS SLYFMQTARL RALLTITLSE LMSDVQVTHM KSDNTLEESG EARRLQQSLS
1301: EMADEAKSVN LLRECGLPDD TLLIIPEKFT ENRWSWAEVK HLSDSLVLAL DASLGHALLG SVMAMDRYAA AESFYKLGMA FAPVPDLHIM WLLHLCDAHQ
1401: EMQSWAEAAQ CAVAVAGVIM QALVARNDGV WSKDHVSALR KICPMVSGEF TTEASAAEVE GYGASKLTVD SAVKYLQLAN KLFSQAELYH FCASILELVI
1501: PVYKSRKAYG QLAKCHTLLT NIYESILDQE SNPIPFIDAT YYRVGFYGEK FGKLDRKEYV YREPRDVRLG DIMEKLSHIY ESRMDSNHIL HIIPDSRQVK
1601: AEDLQAGVCY LQITAVDAVM EDEDLGSRRE RIFSLSTGSV RARVFDRFLF DTPFTKNGKT QGGLEDQWKR RTVLQTEGSF PALVNRLLVT KSESLEFSPV
1701: ENAIGMIETR TTALRNELEE PRSSDGDHLP RLQSLQRILQ GSVAVQVNSG VLSVCTAFLS GEPATRLRSQ ELQQLIAALL EFMAVCKRAI RVHFRLIGEE
1801: DQEFHTQLVN GFQSLTAELS HYIPAILSEL

Arabidopsis Description

SPK1Guanine nucleotide exchange factor SPIKE 1 [Source:UniProtKB/Swiss-Prot;Acc:Q8SAB7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.