CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 1
extracellular 1
cytosol 4
peroxisome 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH07311
KRH18664
KRH31638
KRH69070

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G20950.1	KRG89812	AT2G41100.1	17360592
AT1G20950.1	KRG94975	AT2G41100.1	17360592
AT1G20950.1	KRH18664	AT2G41100.1	17360592
AT1G20950.1	KRH31638	AT4G14640.1	17360592
AT1G20950.1	KRH69070	AT4G14640.1	17360592
AT1G20950.1	KRH07311	AT5G65940.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH49514	Soybean	cytosol, endoplasmic reticulum, nucleus	97.73	97.73
VIT_18s0122g00510.t01	Wine grape	cytosol	85.09	85.23
Solyc12g095760.1.1	Tomato	cytosol, extracellular, nucleus, unclear	84.28	84.28
PGSC0003DMT400075360	Potato	cytosol	84.12	84.12
Solyc04g082880.2.1	Tomato	cytosol, nucleus, plastid, unclear	82.98	83.12
PGSC0003DMT400025919	Potato	cytosol	82.82	82.95
AT1G20950.1	Thale cress	cytosol	82.33	82.74
Bra025858.1-P	Field mustard	cytosol	82.82	82.55
CDY67203	Canola	cytosol	82.82	82.55
CDY67022	Canola	cytosol	82.5	82.23
CDX88295	Canola	cytosol	81.69	81.69
CDY33660	Canola	cytosol	79.09	81.61
CDX96611	Canola	cytosol	78.93	81.44
Bra015734.1-P	Field mustard	cytosol	81.36	81.36
CDX87587	Canola	cytosol	81.36	81.36
AT1G76550.1	Thale cress	cytosol	80.88	80.88
Os02t0714200-01	Rice	cytosol, plasma membrane	80.55	80.55
TraesCS6D01G259500.1	Wheat	golgi	79.25	79.38
TraesCS6B01G307200.1	Wheat	cytosol	79.09	79.22
EES05627	Sorghum	cytosol	79.09	79.22
Zm00001d017830_P001	Maize	cytosol	78.93	78.8
TraesCS6A01G279100.2	Wheat	cytosol	73.91	78.49
Os06t0326400-01	Rice	cytosol, plasma membrane, plastid	78.93	78.3
OQU76351	Sorghum	cytosol	78.28	77.78
TraesCS7D01G228500.1	Wheat	cytosol	75.53	76.27
TraesCS7A01G226000.2	Wheat	cytosol	75.53	76.27
TraesCS7B01G193600.1	Wheat	cytosol	75.2	75.94
HORVU6Hr1G070270.5	Barley	cytosol	79.42	75.15
Zm00001d045919_P001	Maize	mitochondrion	56.24	72.9
Zm00001d051629_P003	Maize	cytosol	73.42	72.6
HORVU7Hr1G047000.11	Barley	cytosol	74.88	69.27
Os09t0298100-00	Rice	plastid	68.4	68.06
HORVU5Hr1G019030.9	Barley	cytosol	68.23	67.68
TraesCS5A01G072700.2	Wheat	cytosol, golgi	67.91	67.47
Os08t0345700-01	Rice	extracellular	67.26	67.26
Zm00001d032386_P003	Maize	cytosol	67.26	67.15
KXG34347	Sorghum	cytosol	66.94	66.83
KXG35340	Sorghum	cytosol	66.61	65.13
OQU80257	Sorghum	cytosol	55.11	63.43
GSMUA_AchrUn_...	Banana	cytosol	13.78	62.96
TraesCS5D01G085500.2	Wheat	golgi	67.75	62.95
TraesCS5B01G078300.1	Wheat	golgi	67.42	62.65
Zm00001d020006_P001	Maize	cytosol	54.94	62.32
Zm00001d035883_P001	Maize	mitochondrion	8.27	53.12
Zm00001d004479_P001	Maize	mitochondrion	16.21	51.55
GSMUA_AchrUn_...	Banana	cytosol	8.27	51.0
Zm00001d008809_P001	Maize	cytosol	18.64	48.52
Zm00001d021503_P001	Maize	cytosol	22.53	48.26
Zm00001d043564_P001	Maize	cytosol	22.53	46.49
Zm00001d003637_P001	Maize	cytosol	4.7	46.03
Zm00001d015529_P001	Maize	mitochondrion	13.13	44.75
Zm00001d032792_P001	Maize	cytosol	8.43	44.44
KRH33517	Soybean	cytosol	6.48	39.6
KRH71798	Soybean	cytosol	6.81	39.25
Zm00001d003638_P001	Maize	mitochondrion	8.91	39.01
Zm00001d036537_P007	Maize	cytosol	7.94	38.89
KRH36515	Soybean	endoplasmic reticulum, mitochondrion	34.04	37.37
KRH11497	Soybean	nucleus	33.71	37.01
KRH51142	Soybean	endoplasmic reticulum, nucleus	33.87	36.8
KRH02012	Soybean	endoplasmic reticulum, plastid	32.41	34.78
KRH15565	Soybean	extracellular	7.13	34.11
Zm00001d037466_P002	Maize	cytosol	6.32	32.5
KRH25835	Soybean	cytosol	10.7	31.58
Zm00001d013450_P001	Maize	cytosol, plastid	12.16	25.77
KRG99300	Soybean	cytosol	11.18	22.77
KRH71808	Soybean	cytosol	6.48	21.86

Protein Annotations

KEGG:00010+2.7.1.11	KEGG:00030+2.7.1.11	KEGG:00051+2.7.1.11	KEGG:00052+2.7.1.11	KEGG:00680+2.7.1.11	Gene3D:1.10.10.480
EntrezGene:100819847	MapMan:2.1.1.1.2	Gene3D:3.40.50.450	Gene3D:3.40.50.460	EMBL:ACUP02012191	ncoils:Coil
EnsemblPlantsGene:GLYMA_20G007400	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0003872	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005975
GO:GO:0006091	GO:GO:0006096	GO:GO:0006139	GO:GO:0008150	GO:GO:0008152	GO:GO:0009056
GO:GO:0009058	GO:GO:0009987	GO:GO:0016301	GO:GO:0016310	GO:GO:0016740	GO:GO:0046835
GO:GO:0046872	GO:GO:0047334	GO:GO:0061615	UniProt:I1ND14	EnsemblPlants:KRG89187	ProteinID:KRG89187
ProteinID:KRG89187.1	HAMAP:MF_01980	PFAM:PF00365	PIRSF:PIRSF005677	InterPro:PKF_sf	PANTHER:PTHR43650
PANTHER:PTHR43650:SF2	MetaCyc:PWY-1042	MetaCyc:PWY-1861	MetaCyc:PWY-5484	MetaCyc:PWY-7385	InterPro:PfpB_PPi_PFK
InterPro:Phosphofructokinase_dom	SUPFAM:SSF53784	TIGRFAMs:TIGR02477	UniParc:UPI000233D8E2	SEG:seg	:

Description

hypothetical protein

Coordinates

chr20:+:685143..690637

Molecular Weight (calculated)

67630.7 Da

IEP (calculated)

6.962

GRAVY (calculated)

-0.062

Length

617 amino acids

Sequence
(BLAST)

001: MDSDYGIPRE LSDLQKIRSL YQPELPPCLQ GTTVRVEFGD ATTTADPTDA LTVCRAFPNT YGHPLAHFLR ATAKVPDAQI ITEFPPIRVG IVFCGRQSPG
101: GHNVIWGIHN ALKIHNPNSV LLGFLGGSEG LFAQKTLEIT DDILSTYKNQ GGYDLLGRTK DQIRTTEQVN AALAACNNLK LDGLVIIGGV TSNTDAAQLA
201: ETFVVAKCPT KVVGVPVTLN GDLKNQFVET NVGFDTICKV NSQLISNVCT DALSAEKYYY FIRLMGRKAS HVALECTLQS HPNMVILGEE VAASKLTLSE
301: IAKQITDAIQ ARAEQDKYHG VILLPEGLIE SIPEVYALLK EIHGLLRQGV EVDKISSQLT PWASALFEFL PPFIRRQLLL YPESDDSAQL SQIETEKLLA
401: YLVEVEINKR LKEGTYKGKK FNAICHFFGY QARGSLPSKF DCDYAYVLGH ICYHILAAGL NGYMATLTNL KNPVNKWKCG AAPIASMMTV KRWSPNPGAT
501: SIGKPAIHPA TVDLRGKAYE LLRQKAQSFL MDDIYRNPGP LQFDGPGADA KVITLSVEDQ DYMGRIKKLQ EYLEQVRTIV KPGCPQEVLK AALSVMASVT
601: EVLAAMSTSY TNTLASL

Best Arabidopsis Sequence Match ( AT1G76550.1 )
(BLAST)

001: MDSDFGIPRE LSPLQQLRSQ YHPELPPCLQ GTTVRVELGD GTTVAKAGDA HIIARAFPHT LGQPLAHFLR ATAKVADAQI ITEHPVKRVG IVFCGRQAPG
101: GHNVVWGLYE ALKVHNAKNT LLGFLGGSEG LFAQKTLEIT DEVLQTYKNQ GGYDMLGRTK DQIRTTEQVN AALKACTDLK LDSLVIIGGV TSNTDAAHLA
201: EFFAEAKCST KVVGVPVTIN GDLKNQFVEA NVGFDTTCKV NSQLISNICT DALSAEKYYY FVRLMGRKHS HVALECTLQS HPNMVILGEE VTASKLTIFD
301: IIKQICDAVQ ARAEQDKNHG VILIPEGLVE SIPELYALLK EIHGLLKEGV QVDNISTQLS SWSSALFEFL PPFIKKQLLL HPESDDSAQL SQIETEKLLA
401: YLVETEMNKR LKEGTYKGKK FNAICHFFGY QARGSLPSKF DCDYAYVLGH VCYHILAAGL NGYMATVTNL KSPVNKWKCG AAPISAMMTV KRWSQNSGST
501: TIGRPVIHPA SVDLKGKAYD LLRQNAQKFL MEDMYRNPGP VQYDGPGADA KAVSLCVEDQ DYMGKIKKLQ EYLDQVRTIV KPGCSQDVLK AALSVMASVT
601: DVLTTISSSS TSGQQFA

Arabidopsis Description

PFP-ALPHA2Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9C9K3]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.