CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, extracellular

Predictor Summary:

nucleus 1
cytosol 3
plasma membrane 1
mitochondrion 1
plastid 1

Predictors

GFP

MS/MS

Papers

extracellular: 25724908
mitochondrion: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 25724908 doi

VC Clarke, PC Loughlin, A Gavrin, C Chen, EM Brear, DA Day, PM Smith

From the ‡University of Sydney, School of Biological Sciences, Sydney Australia;, From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; penny.smith@sydney.edu.au., From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; §Flinders University, School of Biological Sciences, Adelaide Australia.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH33894	Soybean	cytosol	91.34	98.42
KRH68525	Soybean	cytosol	86.16	86.55
KRG96816	Soybean	cytosol	86.16	86.36
PGSC0003DMT400063688	Potato	cytosol	77.5	79.47
Solyc01g099620.2.1	Tomato	cytosol	76.94	79.08
VIT_14s0060g02320.t01	Wine grape	cytosol	79.08	78.64
CDY22644	Canola	plasma membrane	50.51	77.95
CDY65632	Canola	nucleus, peroxisome	12.37	76.39
GSMUA_Achr5P26970_001	Banana	cytosol, peroxisome, plasma membrane	67.94	72.42
Bra031658.1-P	Field mustard	cytosol	67.49	70.92
AT1G09090.2	Thale cress	cytosol	66.25	69.87
CDY06348	Canola	cytosol	63.78	69.49
HORVU4Hr1G086500.1	Barley	cytosol	61.53	64.73
TraesCS4D01G352200.3	Wheat	cytosol	61.3	64.34
TraesCS5A01G527600.1	Wheat	cytosol	61.19	64.23
TraesCS4B01G358800.1	Wheat	cytosol	61.19	64.23
Zm00001d009248_P002	Maize	cytosol	64.23	63.66
Zm00001d040805_P003	Maize	cytosol	63.67	62.96
TraesCS5D01G105900.1	Wheat	cytosol	60.63	62.31
OQU86843	Sorghum	cytosol	64.45	62.01
Os01t0360200-01	Rice	plasma membrane	62.99	61.88
TraesCS5B01G099700.1	Wheat	cytosol	60.52	61.63
TraesCS3A01G182900.1	Wheat	cytosol	62.2	61.38
TraesCS3B01G212900.5	Wheat	cytosol	62.09	61.33
TraesCS3D01G187300.2	Wheat	cytosol	61.98	61.22
TraesCS5A01G093600.1	Wheat	cytosol	60.29	61.12
Os12t0541300-01	Rice	plasma membrane	61.87	60.51
Zm00001d023859_P001	Maize	plastid	60.97	60.09
EES16102	Sorghum	plastid	60.97	59.17
KRH41026	Soybean	mitochondrion	58.94	59.01
KRH59706	Soybean	cytosol	59.28	58.69
KRH63901	Soybean	cytosol, nucleus, plasma membrane	61.08	58.51
KRH54051	Soybean	cytosol, nucleus, plasma membrane	61.3	57.92
HORVU5Hr1G024550.3	Barley	cytosol	55.23	57.16
KRH27880	Soybean	cytosol	57.26	54.91
KRH59983	Soybean	cytosol, nucleus, plasma membrane	57.82	54.62
KRH41250	Soybean	cytosol	57.82	54.62
KRH77593	Soybean	cytosol	56.92	54.58
HORVU3Hr1G037600.3	Barley	cytosol, peroxisome, plastid	26.88	52.07
KRH37549	Soybean	cytosol	51.41	49.25
KRH12608	Soybean	cytosol	51.52	48.93
KRH49079	Soybean	cytosol	46.91	48.54
KRH03132	Soybean	plastid	40.61	43.97
KRH56816	Soybean	plastid	40.49	43.9
KRH03224	Soybean	peroxisome	15.64	19.28
KRH57132	Soybean	plasma membrane	13.72	18.74
KRH48086	Soybean	plasma membrane	14.96	18.52
KRH06603	Soybean	plasma membrane	14.85	18.38
KRH00822	Soybean	plasma membrane	14.62	18.13
KRH35248	Soybean	plasma membrane	14.06	17.53
KRH35246	Soybean	plasma membrane	13.84	17.52
KRH36697	Soybean	plasma membrane	14.4	17.44
KRH11700	Soybean	plasma membrane	14.06	17.08

Protein Annotations

Gene3D:1.10.238.10	MapMan:10.1.1	EntrezGene:100787949	Gene3D:2.40.30.10	Gene3D:3.40.50.80	EMBL:ACUP02012770
InterPro:Cyt_b245_heavy_chain	InterPro:EF-hand-dom_pair	InterPro:EF_Hand_1_Ca_BS	InterPro:EF_hand_dom	InterPro:FAD-bd_8	InterPro:Fd_Rdtase_FAD-bd
InterPro:Fe3_Rdtase_TM_dom	InterPro:Fe_red_NAD-bd_6	EnsemblPlantsGene:GLYMA_20G236200	GO:GO:0003674	GO:GO:0003824	GO:GO:0004601
GO:GO:0005488	GO:GO:0005509	GO:GO:0005575	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987
GO:GO:0016020	GO:GO:0016021	GO:GO:0016491	GO:GO:0050664	GO:GO:0055114	GO:GO:0098869
InterPro:IPR002048	InterPro:IPR017927	UniProt:K7N5A2	EnsemblPlants:KRG92895	ProteinID:KRG92895	ProteinID:KRG92895.1
InterPro:NADPH_Ox	PFAM:PF01794	PFAM:PF08022	PFAM:PF08030	PFAM:PF08414	PRINTS:PR00466
ScanProsite:PS00018	PFscan:PS50222	PFscan:PS51384	PANTHER:PTHR11972	PANTHER:PTHR11972:SF125	InterPro:Riboflavin_synthase-like_b-brl
SMART:SM00054	SUPFAM:SSF47473	SUPFAM:SSF52343	SUPFAM:SSF63380	TMHMM:TMhelix	UniParc:UPI000233DDCC
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr20:+:46851809..46858539

Molecular Weight (calculated)

101365.0 Da

IEP (calculated)

9.669

GRAVY (calculated)

-0.304

Length

889 amino acids

Sequence
(BLAST)

001: MEIHENQHES WSETESTGSR SKQVGFSGPL SGPLSGPLSG PLVSSNKRNS SKNKSARFKD DEEMVEITLD VRDDAVSVQN IRGGDSETAF LASRLEMRPS
101: SFSDRLRQVS RELKRMTSNK AFDRVDRSKS GAARALRGLK FMTKAGTEGW SQVEKRFDEL AIDAKLPKTR FSQCIGMTES KEFAGELFDA LARRRGITSA
201: SITKDQLREF WEQITDQSFD SRLQTFFDMV DKDADGRINE EEVKEIITLS ASANKLSKLK DRAEEYAALI MEELDPDNLG YIELYNLEML LLQAPAQSTN
301: ITTDSRILSQ MLSQKLVPTK EYNPIKRGFR ALAYFVQDNW KRLWVIALWL SICAGLFTWK FIQYKHRAVF HVMGYCVTVA KGGAETTKFN MALILLPVCR
401: NTITWLRSRT KLGAIIPFDD NINFHKVVAF GIAIGVGLHA ISHLTCDFPR LLHATDVEYK PMKQFFGDER PNNYWWFVKG TEGWTGVVMV VLMAIAFILA
501: QPWFRRNRLK LPKPLKKLTG FNAFWYSHHL FVIVYVLFII HGYFLYLSKK WYKKTTWMYL AVPMILYGCE RLLRAFRSGY KSVRILKVAV YPGNVLALHV
601: SKPHGFKYSS GQYIYVNCSD VSPFEWHPFS ITSAPGDDYL SVHIRTLGDW TSQLKGVFAK ACQPASDGQS GLLRADMLQG NNKPRMPRLL IDGPYGAPAQ
701: DYKNYEVILL VGLGIGATPL ISILKDVLNN IKQHKDVEEG AVEKDNKRKP FATKRAYFYW VTREEGSFEW FKGVMNEVEE NDKEGVIELH NYCTSVYEEG
801: DARSALITML QSLHHAKNGV DIVSGTRVKT HFARPNWRNV FKHAAIKHPD QRVGVFYCGA HGLVGELKRL SLDFSRKTST KFDFHKENF

Best Arabidopsis Sequence Match ( AT1G09090.2 )
(BLAST)

001: MREEEMESSS EGETNKISRC KATGSDNPDE DYVEITLEVR DETINTMKAK ATLRSVLSGR LKTMVKSLSF ASRRLDRSKS FGAMFALRGL RFIAKNDAVG
101: RGWDEVAMRF DKLAVEGKLP KSKFGHCIGM VESSEFVNEL FEALVRRRGT TSSSITKTEL FEFWEQITGN SFDDRLQIFF DMVDKNLDGR ITGDEVKEII
201: ALSASANKLS KIKENVDEYA ALIMEELDRD NLGYIELHNL ETLLLQVPSQ SNNSPSSANK RALNKMLSQK LIPTKDRNPV KRFAMNISYF FLENWKRIWV
301: LTLWISICIT LFTWKFLQYK RKTVFEVMGY CVTVAKGSAE TLKFNMALIL LPVCRNTITW LRTKSKLIGS VVPFDDNINF HKVVAFGIAV GIGLHAISHL
401: ACDFPRLLHA KNVEFEPMKK FFGDERPENY GWFMKGTDGW TGVTMVVLML VAYVLAQSWF RRNRANLPKS LKRLTGFNAF WYSHHLFVIV YVLLIVHGYF
501: VYLSKEWYHK TTWMYLAVPV LLYAFERLIR AFRPGAKAVK VLKVAVYPGN VLSLYMSKPK GFKYTSGQYI YINCSDVSPL QWHPFSITSA SGDDYLSVHI
601: RTLGDWTSQL KSLYSKVCQL PSTSQSGLFI ADIGQANNIT RFPRLLIDGP YGAPAQDYRN YDVLLLVGLG IGATPLISII RDVLNNIKNQ NSIERGTNQH
701: IKNYVATKRA YFYWVTREQG SLEWFSEVMN EVAEYDSEGM IELHNYCTSV YEEGDARSAL ITMLQSLHHA KSGIDIVSGT RVRTHFARPN WRSVFKHVAV
801: NHVNQRVGVF YCGNTCIIGE LKRLAQDFSR KTTTKFEFHK ENF

Arabidopsis Description

RBOHBRespiratory burst oxidase homolog protein B [Source:UniProtKB/Swiss-Prot;Acc:Q9SBI0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.