CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, nucleus, cytosol

Predictor Summary:

nucleus 2
cytosol 1
plasma membrane 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89230
KRH14660
KRH49772
KRH50231
KRH71186
KRH73488

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G64060.1	KRG89230	AT4G33950.1	19716822
AT1G64060.1	KRH49772	AT4G33950.1	19716822
AT1G64060.1	KRH50231	AT4G33950.1	19716822
AT1G64060.1	KRH71186	AT4G33950.1	19716822
AT1G64060.1	KRH14660	AT5G21326.1	23162070
AT1G64060.1	KRH73488	AT5G21326.1	23162070

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH41250	Soybean	cytosol	97.24	97.24
KRH77593	Soybean	cytosol	84.27	85.54
KRH27880	Soybean	cytosol	84.27	85.54
VIT_02s0025g00510.t01	Wine grape	cytosol	83.53	83.35
PGSC0003DMT400032088	Potato	cytosol, nucleus, peroxisome	15.41	81.01
Solyc08g081690.2.1	Tomato	cytosol	80.77	78.92
AT1G64060.1	Thale cress	cytosol	78.11	77.86
GSMUA_Achr9P05450_001	Banana	mitochondrion	71.41	77.15
Bra027764.1-P	Field mustard	mitochondrion	77.58	77.0
CDY08347	Canola	mitochondrion	77.05	76.8
GSMUA_Achr6P31850_001	Banana	mitochondrion	71.94	75.9
CDY38202	Canola	mitochondrion	74.5	74.34
EES01429	Sorghum	mitochondrion	72.58	72.35
Zm00001d043543_P002	Maize	mitochondrion	72.37	72.22
HORVU3Hr1G069690.1	Barley	mitochondrion	9.25	71.9
TraesCS3B01G314000.2	Wheat	mitochondrion	71.09	70.87
TraesCS3D01G279900.1	Wheat	mitochondrion	71.09	70.72
Zm00001d038762_P001	Maize	plastid	70.88	70.36
KXG22389	Sorghum	mitochondrion	71.09	69.91
TraesCS1A01G347700.1	Wheat	mitochondrion	70.67	69.2
TraesCS1D01G350600.1	Wheat	mitochondrion	70.88	68.91
TraesCS3A01G280200.1	Wheat	mitochondrion	71.2	68.65
TraesCS1B01G362200.1	Wheat	mitochondrion	70.78	68.52
KRH33894	Soybean	cytosol	54.3	61.94
KRG92895	Soybean	extracellular, mitochondrion	54.62	57.82
KRH68525	Soybean	cytosol	54.09	57.51
KRG96816	Soybean	cytosol	53.77	57.05
KRH37549	Soybean	cytosol	56.11	56.9
KRH12608	Soybean	cytosol	56.22	56.52
Os01t0734200-00	Rice	mitochondrion, nucleus	18.81	55.84
KRH63901	Soybean	cytosol, nucleus, plasma membrane	54.41	55.17
KRH54051	Soybean	cytosol, nucleus, plasma membrane	53.99	53.99
KRH41026	Soybean	mitochondrion	50.8	53.83
KRH59706	Soybean	cytosol	51.12	53.56
KRH49079	Soybean	cytosol	45.59	49.94
KRH03132	Soybean	plastid	40.38	46.28
KRH56816	Soybean	plastid	39.85	45.73
KRH35246	Soybean	plasma membrane	15.09	20.23
KRH03224	Soybean	peroxisome	15.09	19.69
KRH48086	Soybean	plasma membrane	14.67	19.22
KRH57132	Soybean	plasma membrane	13.28	19.2
KRH06603	Soybean	plasma membrane	14.56	19.08
KRH00822	Soybean	plasma membrane	14.35	18.83
KRH35248	Soybean	plasma membrane	14.03	18.51
KRH36697	Soybean	plasma membrane	14.13	18.12
KRH11700	Soybean	plasma membrane	13.92	17.9

Protein Annotations

Gene3D:1.10.238.10	MapMan:10.1.1	EntrezGene:100781551	Gene3D:2.40.30.10	EMBL:ACUP02003346	ncoils:Coil
InterPro:Cyt_b245_heavy_chain	InterPro:EF-hand-dom_pair	InterPro:EF_Hand_1_Ca_BS	InterPro:EF_hand_dom	InterPro:FAD-bd_8	InterPro:Fd_Rdtase_FAD-bd
InterPro:Fe3_Rdtase_TM_dom	InterPro:Fe_red_NAD-bd_6	EnsemblPlantsGene:GLYMA_05G212500	GO:GO:0003674	GO:GO:0003824	GO:GO:0004601
GO:GO:0005488	GO:GO:0005509	GO:GO:0005575	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987
GO:GO:0016020	GO:GO:0016021	GO:GO:0016491	GO:GO:0050664	GO:GO:0055114	GO:GO:0098869
UniProt:I1K6D2	InterPro:IPR002048	InterPro:IPR017927	EnsemblPlants:KRH59983	ProteinID:KRH59983	ProteinID:KRH59983.1
InterPro:NADPH_Ox	PFAM:PF01794	PFAM:PF08022	PFAM:PF08030	PFAM:PF08414	PRINTS:PR00466
ScanProsite:PS00018	PFscan:PS50222	PFscan:PS51384	PANTHER:PTHR11972	PANTHER:PTHR11972:SF66	InterPro:Riboflavin_synthase-like_b-brl
SUPFAM:SSF47473	SUPFAM:SSF52343	SUPFAM:SSF63380	TMHMM:TMhelix	UniParc:UPI00023396EE	SEG:seg

Description

hypothetical protein

Coordinates

chr5:+:39400390..39409178

Molecular Weight (calculated)

106640.0 Da

IEP (calculated)

9.799

GRAVY (calculated)

-0.209

Length

941 amino acids

Sequence
(BLAST)

001: MNGIPRHERR WASDSVPGKA TVSAGTSPGT ESNSAAEEFV EVTLDLQDDD TIVLRSVEPA SVINIDDSVA GSGNQTPASV SRSPTIRRSS SRGFRQFSQE
101: LKAEAVAKAR QFSQELRRFS WSHGHASRAL SSSSAPNGAG AGFETALAAR ALRKQRAQLD RTRSGAHKAL RGLKFISNRS NGVDAWNEVQ SNFDKLATDG
201: FLKRTDFAQC IGMKDSKEFA LELFDALSRK RRLRAEKISR EELFEFWSQI TDQSFDSRLQ IFFDMVDKNE DGRITEVEVK EIIMLSASAN RLSRLKEQAE
301: EYAALIMEEL DPEGLGYIEL WQLETLLLQK DTYLNYSQAL SYTSQALSQN LQGLRKKSPI RRMSRRLVYY LQENWRRLWV LTLWVCIMIG LFTWKFIQYK
401: RKDAFQIMGY CLLAAKGAAE TLKFNMALIL LPVCRNTITW LRSTKLGYSV PFDDNINFHK TIAGAIVIGI ILHAGDHLAC DFPRLVSTSE ESYEKYLKGV
501: FGDRKPSYVD LVKGVEGVTG VLMVVLMIIA FTLATKWFRR NLIKLPKPFS RLTGFNAFWY SHHLFVIVYV LLIIHGIKLY LVHKWYLKTT WMYVAVPVLL
601: YASERILRLF RSGLYTVRLG KVAIYPGNVL TLQMSKPPQF RYKSGQYMFV QCPAVSPFEW HPFSITSAPG DDYLSVHIRQ LGDWTQELKR VFSEACEPPV
701: SGKSGLLRAD ETTKKSLPKL KIDGPYGAPA QDYKKYDVLL LVGLGIGATP FISILKDLLI NIIKMEEMAD SISDISRGSD HSVGSTTDLP SISKIAPKRK
801: KTLKTTNAYF YWVTREQGSF DWFKGVMNEV AELDQRGVIE MHNYLTSVYE EGDARSALIT MVQALNHAKN GVDIVSGTRV RTHFARPNWK KVFSKMCSKH
901: CNGRIGVFYC GAPVLAKELS KLCFEFNEKG PTKFEFHKEH F

Best Arabidopsis Sequence Match ( AT1G64060.1 )
(BLAST)

001: MKPFSKNDRR RWSFDSVSAG KTAVGSASTS PGTEYSINGD QEFVEVTIDL QDDDTIVLRS VEPATAINVI GDISDDNTGI MTPVSISRSP TMKRTSSNRF
101: RQFSQELKAE AVAKAKQLSQ ELKRFSWSRS FSGNLTTTST AANQSGGAGG GLVNSALEAR ALRKQRAQLD RTRSSAQRAL RGLRFISNKQ KNVDGWNDVQ
201: SNFEKFEKNG YIYRSDFAQC IGMKDSKEFA LELFDALSRR RRLKVEKINH DELYEYWSQI NDESFDSRLQ IFFDIVDKNE DGRITEEEVK EIIMLSASAN
301: KLSRLKEQAE EYAALIMEEL DPERLGYIEL WQLETLLLQK DTYLNYSQAL SYTSQALSQN LQGLRGKSRI HRMSSDFVYI MQENWKRIWV LSLWIMIMIG
401: LFLWKFFQYK QKDAFHVMGY CLLTAKGAAE TLKFNMALIL FPVCRNTITW LRSTRLSYFV PFDDNINFHK TIAGAIVVAV ILHIGDHLAC DFPRIVRATE
501: YDYNRYLFHY FQTKQPTYFD LVKGPEGITG ILMVILMIIS FTLATRWFRR NLVKLPKPFD RLTGFNAFWY SHHLFVIVYI LLILHGIFLY FAKPWYVRTT
601: WMYLAVPVLL YGGERTLRYF RSGSYSVRLL KVAIYPGNVL TLQMSKPTQF RYKSGQYMFV QCPAVSPFEW HPFSITSAPE DDYISIHIRQ LGDWTQELKR
701: VFSEVCEPPV GGKSGLLRAD ETTKKSLPKL LIDGPYGAPA QDYRKYDVLL LVGLGIGATP FISILKDLLN NIVKMEEHAD SISDFSRSSE YSTGSNGDTP
801: RRKRILKTTN AYFYWVTREQ GSFDWFKGVM NEVAELDQRG VIEMHNYLTS VYEEGDARSA LITMVQALNH AKNGVDIVSG TRVRTHFARP NWKKVLTKLS
901: SKHCNARIGV FYCGVPVLGK ELSKLCNTFN QKGSTKFEFH KEHF

Arabidopsis Description

RBOHFRespiratory burst oxidase homolog protein F [Source:UniProtKB/Swiss-Prot;Acc:O48538]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.