CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 2
cytosol 2
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, nucleus, plastid
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	plastid
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 23041469
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91376
KRH35440

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G27450.1	KRG91376	AT5G43830.1	21798944
AT4G27450.1	KRH35440	AT5G43830.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH22129	Soybean	nucleus	99.21	99.21
KRH26075	Soybean	endoplasmic reticulum	85.43	85.43
CDY29312	Canola	cytosol	83.46	84.8
Bra026348.1-P	Field mustard	cytosol	83.07	84.4
CDX89248	Canola	cytosol	83.07	84.4
AT4G27450.1	Thale cress	cytosol	83.07	84.4
CDX97384	Canola	cytosol	82.68	84.0
Bra010404.1-P	Field mustard	cytosol	81.89	83.2
CDY14279	Canola	cytosol	81.89	83.2
VIT_19s0014g03130.t01	Wine grape	cytosol	79.53	79.22
Solyc12g013700.1.1	Tomato	cytosol	74.02	74.02
Os04t0679400-01	Rice	cytosol	66.14	65.12
Zm00001d026632_P001	Maize	extracellular	66.54	63.77
KRH10823	Soybean	cytosol	62.99	63.74
KRH21498	Soybean	cytosol	62.99	63.74
EES13111	Sorghum	cytosol, plastid	65.75	63.5
KRH10843	Soybean	nucleus	62.6	63.35
TraesCS2D01G600100.1	Wheat	cytosol	64.57	62.12
TraesCS2A01G586500.1	Wheat	cytosol	64.57	62.12
TraesCSU01G021600.1	Wheat	cytosol	64.17	61.74
GSMUA_Achr3P14300_001	Banana	cytosol	64.17	61.51
KRH20474	Soybean	nucleus	62.2	61.48
GSMUA_AchrUn_...	Banana	cytosol	58.27	61.16
GSMUA_Achr10P...	Banana	cytosol	63.78	60.9
KRH33539	Soybean	cytosol	35.83	56.52
KRH63496	Soybean	cytosol	42.52	56.25
KRG89645	Soybean	cytosol	40.55	53.09
KRH21458	Soybean	cytosol	26.38	51.94
KRH50016	Soybean	cytosol, nucleus, plastid	62.6	50.64
KRH35440	Soybean	cytosol, nucleus	46.46	47.2
KRG91376	Soybean	cytosol, nucleus	46.06	46.8
KRH02111	Soybean	cytosol	40.94	44.07
KRH20897	Soybean	nucleus	40.55	43.64
KRH51038	Soybean	plastid	39.76	42.98
KRH10856	Soybean	cytosol	42.91	42.91
HORVU2Hr1G118550.2	Barley	cytosol	38.98	40.41
KRH38367	Soybean	extracellular	18.11	38.02
KRH11390	Soybean	nucleus	40.55	35.15
HORVU2Hr1G118570.2	Barley	cytosol	26.38	24.72

Protein Annotations

EMBL:ACUP02007786	EMBL:BT097244	EnsemblPlants:KRH27213	EnsemblPlantsGene:GLYMA_12G222400	EntrezGene:547601	Gene3D:3.60.20.10
InterPro:DUF3700	InterPro:IPR029055	InterPro:Ntn_hydrolases_N	PANTHER:PTHR11772	PANTHER:PTHR11772:SF15	PFAM:PF12481
ProteinID:KRH27213	ProteinID:KRH27213.1	SEG:seg	SMART:SM01172	SUPFAM:SSF56235	UniParc:UPI0001B16875
UniProt:C6THW8	MapMan:35.1	:	:	:	:

Description

hypothetical protein

Coordinates

chr12:-:38186066..38188213

Molecular Weight (calculated)

28035.4 Da

IEP (calculated)

6.595

GRAVY (calculated)

-0.214

Length

254 amino acids

Sequence
(BLAST)

001: MLAIFHKAFA HPPEELNSPA SYKGSKKPKV PEETLKDFLS HHPHNTCSMS FGDAAVLAYV RPDQSFSRHQ RLFCGIDDIY CLFLGSLNNL SLLNKQYGLS
101: KSTDEAMFVI EAYKTLRDRG PYPADQVVKE LDGSFAFVVY DSKVGSVFAA LGSDGGVKLY WGIAADGSVV ISDDLEVIKE GCAKSFAPFP TGCMFHSEGG
201: LMSFEHPMNK LKAMPRVDSE GAMCGANFKV DKFARVNSIP RVGSQSNWME WDQH

Best Arabidopsis Sequence Match ( AT4G27450.1 )
(BLAST)

001: MLAIFHEAFA HPPEELNSPA SEKCSKQPKL PEETLNDFLL RYPLNTFSMS FGQAAVLAYV RPSASFSIHQ RLFCGFDDIY CLFFGSLNNL CQLNKQYGLT
101: KTTNEAMFVI EAYRTLRDRG PYPADQVVKD LDGSFSFVVY DSKAGSVFTA LGSDGGVKLY WGIAADGSVV ISDDLDVIKE GCAKSFAPFP TGCMFHSEGG
201: LMSFEHPMNK IKAMPRVDSE GVLCGANFKV DVYNRVNSIP RRGSEANWSL

Arabidopsis Description

AT4g27450/F27G19_50 [Source:UniProtKB/TrEMBL;Acc:Q93V62]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.