CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

plastid 3
mitochondrion 5
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	plastid
EpiLoc:	mitochondrion
iPSORT:	mitochondrion
MultiLoc:	mitochondrion
Plant-mPloc:	plastid
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	plastid
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH04597
KRH28456
KRH57934
KRH63322
KRH77015

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G58730.1	KRH04597	AT2G23420.1	21798944
AT3G58730.1	KRH28456	AT2G23420.1	21798944
AT3G58730.1	KRH57934	AT2G23420.1	21798944
AT3G58730.1	KRH63322	AT2G23420.1	21798944
AT3G58730.1	KRH77015	AT2G23420.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH47345	Soybean	endoplasmic reticulum	98.06	98.06
GSMUA_Achr1P17380_001	Banana	mitochondrion	82.17	80.61
PGSC0003DMT400074548	Potato	mitochondrion	81.4	80.46
Solyc01g073690.2.1	Tomato	nucleus	75.97	79.67
Bra003352.1-P	Field mustard	mitochondrion	79.46	78.54
AT3G58730.1	Thale cress	mitochondrion	78.68	77.78
Bra007415.1-P	Field mustard	mitochondrion	78.68	77.78
CDX67762	Canola	mitochondrion	60.47	77.23
CDX98325	Canola	mitochondrion	60.08	76.73
CDY00584	Canola	mitochondrion	60.08	76.73
CDX89001	Canola	mitochondrion	60.08	76.35
CDY49248	Canola	mitochondrion	59.69	76.24
GSMUA_AchrUn_...	Banana	cytosol	60.85	76.21
CDX71929	Canola	mitochondrion	59.69	75.86
Bra014555.1-P	Field mustard	mitochondrion	59.3	75.74
TraesCS2D01G521600.1	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	78.29	74.81
TraesCS2B01G549600.1	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	78.29	74.81
Os04t0643100-01	Rice	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	77.91	74.72
EES12954	Sorghum	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	77.52	74.35
TraesCS2A01G520400.1	Wheat	golgi	77.52	74.07
Zm00001d024242_P001	Maize	mitochondrion, plastid	77.13	73.98
Zm00001d002105_P001	Maize	plasma membrane	76.74	73.61
VIT_12s0028g01260.t01	Wine grape	cytosol	83.72	72.24
TraesCS6D01G135900.1	Wheat	mitochondrion	63.95	62.26
TraesCS6A01G146600.1	Wheat	mitochondrion	63.18	61.51
Zm00001d024465_P001	Maize	plastid	63.18	61.28
TraesCS6B01G174800.1	Wheat	mitochondrion	63.18	60.82
OQU79969	Sorghum	mitochondrion	46.9	58.74
PGSC0003DMT400080629	Potato	cytosol	25.58	58.41
HORVU6Hr1G028720.1	Barley	vacuole	61.63	57.4

Protein Annotations

EMBL:ACUP02004941	EnsemblPlants:KRH44559	EnsemblPlantsGene:GLYMA_08G218500	EntrezGene:100801612	GO:GO:0003674	GO:GO:0003824
GO:GO:0005215	GO:GO:0006810	GO:GO:0008150	GO:GO:0016787	GO:GO:0042626	GO:GO:0055085
InterPro:V_ATPase_D	ncoils:Coil	PANTHER:PTHR11671	PFAM:PF01813	ProteinID:KRH44559	ProteinID:KRH44559.1
TIGRFAMs:TIGR00309	UniParc:UPI00023390F8	UniProt:K7L827	MapMan:24.1.1.2.4	:	:

Description

hypothetical protein

Coordinates

chr8:-:17768350..17769823

Molecular Weight (calculated)

28894.0 Da

IEP (calculated)

10.184

GRAVY (calculated)

-0.360

Length

258 amino acids

Sequence
(BLAST)

001: MSGQTQRLNV VPTVTMLGVV KARLVGATRG HALLKKKSDA LTVQFRQILK KIVSTKESMG DIMKTSSFAL TEAKYVAGDN IKHVVLENVK EASLRVRSRQ
101: ENVAGVKLPK FDYTADADAT KNDLTGLARG GQQVQQCRAA YIKAIEVLVE LASLQTSFLT LDEAIKTTNR RVNALENVVK PRLENTISYI KGELDELERE
201: DFFRLKKIQG YKKREIERQM LLQNNSNVET NLPLRKGVSY NSAHNLLSVG DKDEDIIF

Best Arabidopsis Sequence Match ( AT3G58730.1 )
(BLAST)

001: MAGQNARLNV VPTVTMLGVM KARLVGATRG HALLKKKSDA LTVQFRALLK KIVTAKESMG DMMKTSSFAL TEVKYVAGDN VKHVVLENVK EATLKVRSRT
101: ENIAGVKLPK FDHFSEGETK NDLTGLARGG QQVRACRVAY VKAIEVLVEL ASLQTSFLTL DEAIKTTNRR VNALENVVKP KLENTISYIK GELDELERED
201: FFRLKKIQGY KRREVERQAA NAKEFAEEMV LEDISMQRGI SINAARNFLV GGAEKDSDII F

Arabidopsis Description

VHA-DV-type proton ATPase subunit D [Source:UniProtKB/Swiss-Prot;Acc:Q9XGM1]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.