CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

extracellular 5
endoplasmic reticulum 5
vacuole 5
plasma membrane 4
golgi 4
mitochondrion 1
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
Plant-mPloc:	extracellular
Predotar:	secretory
PProwler:	secretory
TargetP:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	endoplasmic reticulum

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH53951	Soybean	cytosol, nucleus, vacuole	83.48	92.48
VIT_05s0029g01090.t01	Wine grape	endoplasmic reticulum, vacuole	75.49	77.7
CDY43760	Canola	vacuole	54.16	76.74
CDX84449	Canola	vacuole	54.05	76.59
CDX81200	Canola	vacuole	53.39	76.13
CDY60045	Canola	cytosol, nucleus, vacuole	53.17	75.82
GSMUA_Achr1P22850_001	Banana	cytosol	49.12	75.34
Solyc04g049070.2.1	Tomato	plastid	74.07	73.51
Bra037754.1-P	Field mustard	vacuole	67.4	73.25
Bra024240.1-P	Field mustard	vacuole	73.74	73.18
PGSC0003DMT400013692	Potato	vacuole	73.96	73.16
AT5G63840.2	Thale cress	plasma membrane, vacuole	73.96	71.31
TraesCS4D01G244900.1	Wheat	cytosol	64.11	71.03
Os03t0216600-01	Rice	plastid	65.54	69.49
HORVU4Hr1G067520.2	Barley	plasma membrane, vacuole	69.26	69.18
TraesCS4B01G244500.1	Wheat	golgi	69.04	68.89
EER95268	Sorghum	vacuole	68.93	68.7
Zm00001d028227_P007	Maize	vacuole	68.71	68.48
Zm00001d048080_P002	Maize	plasma membrane	68.27	68.2
TraesCS4A01G066900.2	Wheat	golgi	68.27	67.97
VIT_16s0039g01030.t01	Wine grape	extracellular, plasma membrane	76.81	58.84
KRH11838	Soybean	vacuole	20.13	28.62
KRH11840	Soybean	cytosol, nucleus	13.46	26.06
KRH36850	Soybean	nucleus	25.49	25.98
KRH11839	Soybean	extracellular	25.16	24.55
KRH75375	Soybean	endoplasmic reticulum	24.29	23.92
KRH45278	Soybean	endoplasmic reticulum	23.74	23.43
KRH35960	Soybean	endoplasmic reticulum	23.63	23.35
KRH62685	Soybean	nucleus	24.18	20.99
KRH66765	Soybean	plastid	13.89	14.46

Protein Annotations

EntrezGene:100817168	MapMan:18.1.1.6.2.1	Gene3D:2.60.40.1180	Gene3D:2.60.40.1760	Gene3D:3.20.20.80	EMBL:ACUP02002734
EnsemblPlantsGene:GLYMA_04G209000	GO:GO:0003674	GO:GO:0003824	GO:GO:0004553	GO:GO:0005488	GO:GO:0005975
GO:GO:0008150	GO:GO:0008152	GO:GO:0016787	GO:GO:0016798	GO:GO:0030246	InterPro:Gal_mutarotase_sf_dom
InterPro:Glyco_hydro_31	InterPro:Glyco_hydro_31_AS	InterPro:Glyco_hydro_31_CS	InterPro:Glyco_hydro_31_N_dom	InterPro:Glyco_hydro_b	InterPro:Glycoside_hydrolase_SF
UniProt:I1JXY2	InterPro:IPR013780	EnsemblPlants:KRH63987	ProteinID:KRH63987	ProteinID:KRH63987.1	PFAM:PF01055
PFAM:PF13802	ScanProsite:PS00129	ScanProsite:PS00707	PANTHER:PTHR22762	PANTHER:PTHR22762:SF54	SUPFAM:SSF51011
SUPFAM:SSF51445	SUPFAM:SSF74650	SignalP:SignalP-noTM	UniParc:UPI00023397F2	SEG:seg	:

Description

hypothetical protein

Coordinates

chr4:-:48116782..48122644

Molecular Weight (calculated)

103969.0 Da

IEP (calculated)

6.369

GRAVY (calculated)

-0.413

Length

914 amino acids

Sequence
(BLAST)

001: MRNETLRLIL LLLLCSHLHS VLSWKKEEFR TCHQTPFCKR ARSRAPGSSS LIATDVTISH GDLTAKLTPK HDSQSETKPL LLTLSVYQRG ILRLKIDEDP
101: SLSPPKKRFE VPDVIVSEFP STKLWLPKIS SVENGLSSSV YLSDGHSAVL RHDPFELFIR DDSSGDRVIS LNSHDLFDFE QLKHKSEDDN WEEQFRSHTD
201: RRPYGPQSIS FDVSFYGADF VYGIPERAAS LALKPTRGPN VDESEPYRLF NLDVFEYIHD SPFGLYGSIP FMVSHGKARG SSGFFWLNAA EMQIDVLAPG
301: WDAESGIALP SHRIDTFWMS EAGVVDAFFF IGPNPKDVLR QYTAVTGTPA MPQLFSIAYH QCRWNYRDEE DVEHVDSKFD ELDIPYDVLW LDIEHTDGKR
401: YFTWDRALFP HPEEMQRKLA SKGRHMVTIV DPHIKRDENF HLHKEASQKG YYVKDASGND FDGWCWPGSS SYPDTLNPEI RSWWADKFSY QSYEGSTPSL
501: YIWNDMNEPS VFNGPEVTMP RDVTHYGGVE HRELHNAYGY YFHMATANGL LKRGEGNDRP FVLSRALFAG SQRYGAVWTG DNTADWDHLR VSIPMVLTLG
601: LTGMSFSGAD IGGFFGNPEP ELLVRWYQLG AYYPFFRAHA HHDTKRREPW LFGERNTELI KDAIHVRYAL LPYFYTLFRE ANTTGVPVVR PLWMEFPSDE
701: ATFSNDETFM VGSSILVQGI YTERAKHASV YLPGKQSWYD LRTGAVYKGG VTHKLEVTEE SIPAFQRAGT IIARKDRFRR SSTQMANDPY TLVVALNSSQ
801: AAEGELYIDD GSSFNFLQGG YIHRRFIFSN GKLTSIDLAP ASSSKGRYPS DAFIERIILL GHAPSSKNAL IEPSNQKVDI ELGPLWVLRA RAPAVTTIRR
901: PNVRVAEDWT ITVI

Best Arabidopsis Sequence Match ( AT5G63840.2 )
(BLAST)

001: MIVFLSVVFW LSSLLPQSKD FHEQGTKMRS LLFVLSLICF CSQTALSWKK EEFRSCDQTP FCKRARSRTP GACSLIVGDV SITDGDLVAK LLPKAPNQGD
101: GDQIKPLILS LSVYKDGIVR LKIDEDHSLN PPKKRFQVPD VVVSEFEEKK IWLQKVATET ISGDTSPSSV VYVSDGYEAV VRHDPFEVYV REKSGDRRRV
201: VSLNSHGLFD FEQLGRKTEG DNWEEKFRTH TDSRPSGPQS ISFDVSFYDS SFVYGIPEHA TSFALKPTKG PGVEESEPYR LFNLDVFEYD HESPFGLYGS
301: IPFMVSHGKS GKTSGFFWLN AAEMQIDVLA NGWDAESGIS LPSSHSRIDT FWMSEAGIVD TFFFVGPEPK DVVKQYASVT GTSAMPQLFA TGYHQCRWNY
401: KDEEDVAQVD SKFDEHDIPY DVLWLDIEHT DGKRYFTWDS VLFPHPEEMQ KKLAAKGRKM VTIVDPHIKR DDSYFLHKEA TQMGYYVKDS SGKDFDGWCW
501: PGSSSYIDML SPEIRKWWGG RFSYKNYVGS TPSLYTWNDM NEPSVFNGPE VTMPRDALHV GGVEHREVHN AYGYYFHMAT SDGLVMREEG KDRPFVLSRA
601: IFPGTQRYGA IWTGDNTAEW EHLRVSIPMI LTLGLTGITF SGADIGGFFG NPEPELLVRW YQVGAYYPFF RGHAHHDTKR REPWLFGERN TELMRDAIHT
701: RYTLLPYFYT LFREANVTGV PVVRPLWMEF PQDEATFSND EAFMVGSGLL VQGVYTKGTT QASVYLPGKE SWYDLRNGKT YVGGKTHKMD APEESIPAFQ
801: KAGTIIPRKD RFRRSSSQMD NDPYTLVVAL NSSQEAEGEL YIDDGKSFEF RRGSYIHRRF VFSKGVLTST NLAPPEARLS SQCLIDRIIL LGHSSGPKSA
901: LVEPLNQKAE IEMGPLRMGG LVASSGTKVL TIRKPGVRVD QDWTVKIL

Arabidopsis Description

RSW3Glycosyl hydrolases family 31 protein [Source:TAIR;Acc:AT5G63840]
SUBAcon: [plasma membrane,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.