Factsheet for Os02t0181900-01

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 2
cytosol 1
mitochondrion 7

Predictors

GFP

MS/MS

Papers

mitochondrion: 20141629

plasma membrane: 27800704
plastid: 27992503

gfp PMID: 20141629 doi

A Singh, U Singh, D Mittal, A Grover

Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi-110021, India.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0708400-01
Os03t0596900-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G25140.1	Os02t0708400-01	AT3G27960.1	21798944
AT2G25140.1	Os03t0596900-01	AT4G26630.1	25387881

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d015520_P001	Maize	mitochondrion	91.76	91.3
EES06386	Sorghum	mitochondrion	91.76	91.11
HORVU6Hr1G028690.1	Barley	mitochondrion	89.01	88.92
TraesCS6D01G135600.1	Wheat	golgi	88.81	88.09
TraesCS6A01G146400.1	Wheat	mitochondrion	88.81	88.09
TraesCS6B01G174500.1	Wheat	mitochondrion	88.4	87.69
GSMUA_Achr9P22420_001	Banana	cytosol	73.55	81.88
Solyc06g011380.2.1	Tomato	cytosol, plastid	16.99	81.46
KRH52355	Soybean	cytosol, mitochondrion	50.36	76.04
VIT_04s0008g05870.t01	Wine grape	mitochondrion	73.35	74.95
KRH61688	Soybean	mitochondrion	74.16	74.85
CDX76768	Canola	mitochondrion	73.86	74.61
CDY56280	Canola	mitochondrion	73.86	74.54
Bra007816.1-P	Field mustard	mitochondrion	73.75	74.44
AT2G25140.1	Thale cress	mitochondrion	72.33	73.76
CDY64721	Canola	cytosol	17.4	70.08
CDY53470	Canola	mitochondrion	53.61	66.46
Bra032044.1-P	Field mustard	mitochondrion	53.51	66.25
Os03t0426900-01	Rice	plastid	65.11	65.44
CDY68892	Canola	mitochondrion, plastid	55.04	65.26
Os04t0397100-01	Rice	plastid	19.43	47.39
Os05t0519700-01	Rice	nucleus	42.42	45.72
Os11t0269000-00	Rice	cytosol, plasma membrane	18.01	44.92
Os12t0230100-01	Rice	plastid	40.18	42.98
Os02t0537400-01	Rice	cytosol	19.13	42.92
Os02t0297132-00	Rice	cytosol	15.56	40.58
Os02t0526400-01	Rice	cytosol, plasma membrane	33.37	39.9
Os11t0267400-01	Rice	plasma membrane	37.23	39.27

Protein Annotations

Gene3D:1.10.1780.10	Gene3D:1.10.8.60	MapMan:26.4.2.4	Gene3D:3.40.50.300	EntrezGene:4328515	InterPro:AAA+_ATPase
EMBL:AK287906	InterPro:ATPase_AAA_core	ProteinID:BAF08010.2	ProteinID:BAS77313.1	InterPro:Chaperonin_ClpB	InterPro:ClpA/B
InterPro:ClpA/B_CS1	InterPro:ClpA/B_CS2	InterPro:Clp_ATPase_C	InterPro:Clp_N	InterPro:Clp_N_dom_sf	ncoils:Coil
GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005739	GO:GO:0006950	GO:GO:0008150	GO:GO:0008152
GO:GO:0009408	GO:GO:0009628	GO:GO:0009987	GO:GO:0019538	GO:GO:0042026	InterPro:IPR036628
EnsemblPlantsGene:Os02g0181900	EnsemblPlants:Os02t0181900-01	InterPro:P-loop_NTPase	PFAM:PF00004	PFAM:PF02861	PFAM:PF07724
PFAM:PF10431	PRINTS:PR00300	ScanProsite:PS00870	ScanProsite:PS00871	PANTHER:PTHR11638	PANTHER:PTHR11638:SF86
UniProt:Q0E3C8	SMART:SM00382	SMART:SM01086	SUPFAM:SSF52540	SUPFAM:SSF81923	TIGRFAMs:TIGR03346
UniParc:UPI0001C7DD2A	RefSeq:XP_015627597.1	SEG:seg	:	:	:

Description

CLASS I CLP ATPASE B-MSimilar to ClpB. (Os02t0181900-01)

Coordinates

chr2:-:4567630..4573049

Molecular Weight (calculated)

109254.0 Da

IEP (calculated)

7.249

GRAVY (calculated)

-0.350

Length

983 amino acids

Sequence
(BLAST)

001: MSRATAVSRL ARAARAAAAA RRHHAGGRDP LRALASLAGD ASASAGGGAR RPAWFAPPMG RLGGGGLLVP PPPPQRRLFH PTQAARYSTS SSSQITPGEF
101: TEMAWEGVVG AVDAARMSKQ QVVEAEHLMK ALLEQKDGLA RRIFSKAGID NTSVLQATDE FISRQPKVVG DTSGPIIGSS FVSILDNARK HKKEYADEFV
201: SVEHILRAFT EDKRFGQQLF RDLKIGENEL KEAISAVRGS QRVTDQNPEG KYQALEKYGI DMTELARRGK LDPVIGRDDE VRRCIQILCR RTKNNPVIIG
301: EPGVGKTAIA EGLAQRIVRG DVPEPLQNRK LISLDMGALL AGAKFQGQFE ERLKAVLKEI TASNGQIILF IDEIHTIVGA GAAGGAMDAG NLLKPMLGRG
401: ELRCIGATTL DEYRKYIEKD AALERRFQQV YCGEPAVEDT ISILRGLRER YELHHGVKIS DGALVSAAVL SDRYITGRFL PDKAIDLVDE AAAKLKMEIT
501: SKPIELDEVD REIIRLEMEK LSLKNDTDKA SKQRLSKLEA DLESLKQKQK NLSEHWEYEK SLMTRIRSIK EETDRVNLEI EAAEREYDLN RAAELKYGTL
601: LSLQKQLEEA ENKLMEFQQS GKSMLREEVT DVDIAEIVSK WTGIPVSNLQ QSEKEKLLLL EDVLHKRVIG QDIAVKSVAN AIRRSRAGLS DPNRPIASLM
701: FMGPTGVGKT ELGKTLAEFL FNTENALIRI DMSEYMEKHA VSRLVGAPPG YIGYGEGGQL TEAVRRRPYS VVLFDEIEKA HQDVFNILLQ LLDDGRITDS
801: QGRTVSFTNC VIIMTSNIGS PLILDTLRNT SDSKEAVYEI MKKQVIDMAR QSFRPEFLNR IDEYIVFQPL DTTEINRIVE IQLNRVKNRL RQQKIHLQYT
901: PEAVEHLGSL GFDPNYGARP VKRVIQQMVE NEIALSVLKG DFKEDDTVLV DVSSVAIAKG LAPQKKLVLQ RLENANLELV AND

Best Arabidopsis Sequence Match ( AT2G25140.1 )
(BLAST)

001: MALRRLSKSV SSAIKAQYTL SRPSPLLRSR SLSSSPHYTS IGRPTNSFIG KINNSSITHA TTTHGQLFPL SSPRRFCTTT AQVNQNEFTE MAWEGLINAF
101: DAARESKQQI VESEHLMKAL LEQKDGMARK IFTKAGIDNS SVLQATDLFI SKQPTVSDAS GQRLGSSLSV ILENAKRHKK DMLDSYVSVE HFLLAYYSDT
201: RFGQEFFRDM KLDIQVLKDA IKDVRGDQRV TDRNPESKYQ ALEKYGNDLT EMARRGKLDP VIGRDDEIRR CIQILCRRTK NNPVIIGEPG VGKTAIAEGL
301: AQRIVRGDVP EPLMNRKLIS LDMGSLLAGA KFRGDFEERL KAVMKEVSAS NGQTILFIDE IHTVVGAGAM DGAMDASNLL KPMLGRGELR CIGATTLTEY
401: RKYIEKDPAL ERRFQQVLCV QPSVEDTISI LRGLRERYEL HHGVTISDSA LVSAAVLADR YITERFLPDK AIDLVDEAGA KLKMEITSKP TELDGIDRAV
501: IKLEMEKLSL KNDTDKASKE RLQKIENDLS TLKQKQKELN VQWEKEKSLM TKIRSFKEEI DRVNLEIESA EREYDLNRAA ELKYGTLLSL QRQLEEAEKN
601: LTNFRQFGQS LLREVVTDLD IAEIVSKWTG IPLSNLQQSE REKLVMLEEV LHHRVIGQDM AVKSVADAIR RSRAGLSDPN RPIASFMFMG PTGVGKTELA
701: KALAGYLFNT ENAIVRVDMS EYMEKHSVSR LVGAPPGYVG YEEGGQLTEV VRRRPYSVVL FDEIEKAHPD VFNILLQLLD DGRITDSQGR TVSFKNCVVI
801: MTSNIGSHHI LETLRNNEDS KEAVYEIMKR QVVELARQNF RPEFMNRIDE YIVFQPLDSN EISKIVELQM RRVKNSLEQK KIKLQYTKEA VDLLAQLGFD
901: PNYGARPVKR VIQQMVENEI AVGILKGDFA EEDTVLVDVD HLASDNKLVI KKLESNASAE EMAA

Arabidopsis Description

CLPB4Chaperone protein ClpB4, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q8VYJ7]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.