CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 5
cytosol 1
mitochondrion 4
nucleus 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
mitochondrion: 27780359

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG93305
KRG98502
KRG98507
KRH46360
KRH46364
KRH56614

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G25140.1	KRG93305	AT3G27960.1	21798944
AT2G25140.1	KRH56614	AT3G27960.1	21798944
AT2G25140.1	KRG98502	AT4G26630.1	25387881
AT2G25140.1	KRG98507	AT4G26630.1	25387881
AT2G25140.1	KRH46360	AT4G26630.1	25387881
AT2G25140.1	KRH46364	AT4G26630.1	25387881

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH52355	Soybean	cytosol, mitochondrion	62.12	92.93
Solyc06g011380.2.1	Tomato	cytosol, plastid	18.28	86.83
GSMUA_Achr9P22420_001	Banana	cytosol	75.98	83.81
VIT_04s0008g05870.t01	Wine grape	mitochondrion	81.11	82.12
Bra007816.1-P	Field mustard	mitochondrion	79.06	79.06
CDY56280	Canola	mitochondrion	78.95	78.95
CDX76768	Canola	mitochondrion	78.75	78.83
AT2G25140.1	Thale cress	mitochondrion	76.9	77.7
EES06386	Sorghum	mitochondrion	76.9	75.66
HORVU6Hr1G028690.1	Barley	mitochondrion	75.77	75.0
TraesCS6B01G174500.1	Wheat	mitochondrion	75.46	74.17
Os02t0181900-01	Rice	mitochondrion	74.85	74.16
TraesCS6D01G135600.1	Wheat	golgi	75.36	74.07
TraesCS6A01G146400.1	Wheat	mitochondrion	75.26	73.97
CDY64721	Canola	cytosol	17.76	70.9
CDY53470	Canola	mitochondrion	56.16	68.98
Bra032044.1-P	Field mustard	mitochondrion	56.06	68.77
KRH01254	Soybean	nucleus, plastid	67.76	67.76
CDY68892	Canola	mitochondrion, plastid	57.6	67.67
KRH44979	Soybean	nucleus, plastid	67.56	67.56
KRH18417	Soybean	nucleus	66.94	66.67
KRG93616	Soybean	nucleus	66.84	66.56
KRH63249	Soybean	mitochondrion	11.6	58.25
KRH54672	Soybean	endoplasmic reticulum	39.73	51.39
KRH03117	Soybean	cytosol	23.61	50.66
KRH46928	Soybean	endoplasmic reticulum	5.95	48.74
KRH56831	Soybean	endoplasmic reticulum	44.25	47.31
KRH59753	Soybean	endoplasmic reticulum	41.17	43.63
KRH41069	Soybean	mitochondrion, plastid	41.07	43.53
KRH63851	Soybean	plastid	41.07	43.38
KRH54115	Soybean	plastid	41.07	43.38
KRH63903	Soybean	plastid	34.39	35.41
KRH54048	Soybean	plastid	33.98	34.84
Zm00001d008786_P001	Maize	plastid	4.62	25.28

Protein Annotations

Gene3D:1.10.1780.10	Gene3D:1.10.8.60	EntrezGene:100782242	MapMan:26.4.2.4	Gene3D:3.40.50.300	InterPro:AAA+_ATPase
EMBL:ACUP02002181	InterPro:ATPase_AAA_core	InterPro:Chaperonin_ClpB	InterPro:ClpA/B	InterPro:ClpA/B_CS1	InterPro:ClpA/B_CS2
InterPro:Clp_ATPase_C	InterPro:Clp_N	InterPro:Clp_N_dom_sf	ncoils:Coil	EnsemblPlantsGene:GLYMA_04G062200	GO:GO:0000166
GO:GO:0003674	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0006950	GO:GO:0008150	GO:GO:0008152	GO:GO:0009408	GO:GO:0009628
GO:GO:0009987	GO:GO:0016485	GO:GO:0019538	GO:GO:0042026	UniProt:I1JU82	InterPro:IPR036628
EnsemblPlants:KRH61688	ProteinID:KRH61688	ProteinID:KRH61688.1	InterPro:P-loop_NTPase	PFAM:PF00004	PFAM:PF02861
PFAM:PF07724	PFAM:PF10431	PRINTS:PR00300	ScanProsite:PS00870	ScanProsite:PS00871	PANTHER:PTHR11638
PANTHER:PTHR11638:SF86	SMART:SM00382	SMART:SM01086	SUPFAM:SSF52540	SUPFAM:SSF81923	TIGRFAMs:TIGR03346
UniParc:UPI0002339C1A	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr4:-:5112212..5119686

Molecular Weight (calculated)

109228.0 Da

IEP (calculated)

7.495

GRAVY (calculated)

-0.402

Length

974 amino acids

Sequence
(BLAST)

001: MATRRTPTLT KSVFAAVTAS RTSRSRSHSA RRLFPAIPRA SENSLSRSQI IDPTNVASAK FLSRSFTRTF HATNPSLRSA ASSQVAQTDF TDMAWEGIVG
101: AVDAARVSKQ QIVESEHLMK ALLEQKDGLA RRIFTKAGLD NTSVLQATED FIAKQPKVTG DTSGPVVGSH FSSLLDNSRK YKKEMGDEYV SVEHLLLAFH
201: SDKRFGQQLF KNLQLSEKAL KDAVQAVRGS QRVTDQNPEG KYEALDKYGN DLTELARRGK LDPVIGRDDE IRRCIQILSR RTKNNPVIIG EPGVGKTAIA
301: EGLAQRIVRG DVPEPLMNRK LISLDMGSLL AGAKYRGDFE ERLKAVLKEV TASNGQIILF IDEIHTVVGA GATSGAMDAG NLLKPMLGRG ELRCIGATTL
401: NEYRKYIEKD PALERRFQQV FCSQPSVEDT ISILRGLRER YELHHGVKIS DSALVSAAVL ADRYITERFL PDKAIDLVDE AAAKLKMEIT SKPTELDEID
501: RAILKLEMEK LSLKNDTDKA SKERLSKLEN DLSLLKQKQK ELTEQWDSEK VFMTRIRSIK EEIDRVNLEM EAAERDYDLN RAAELKYGTL MSLQRQLEEA
601: EKNLSDFRNS GQSLLREEVT DLDITEIVSK WTGIPLSNLQ QTEREKLVLL EQVLHKRVVG QDTAVKSVAD AIRRSRAGLS DPNRPIASFM FMGPTGVGKT
701: ELAKALAGYL FNTENALVRI DMSEYMEKHA VSRLVGAPPG YVGYEEGGQL TEVVRRRPYS VVLFDEIEKA HHDVFNILLQ LLDDGRITDS QGRTVSFTNC
801: VVIMTSNIGS HYILDTLRST QDDKTAVYDQ MKRQVVELAR QTFHPEFMNR IDEYIVFQPL DSEQISKIVE LQMERVKNRL KQKKIDLHYT EKAVKLLGVL
901: GFDPNFGARP VKRVIQQLVE NEIAMGVLRG DFKEEDSIIV DADVTLSGKE RSPLNKLLIK KLDSPDADAM VVND

Best Arabidopsis Sequence Match ( AT2G25140.1 )
(BLAST)

001: MALRRLSKSV SSAIKAQYTL SRPSPLLRSR SLSSSPHYTS IGRPTNSFIG KINNSSITHA TTTHGQLFPL SSPRRFCTTT AQVNQNEFTE MAWEGLINAF
101: DAARESKQQI VESEHLMKAL LEQKDGMARK IFTKAGIDNS SVLQATDLFI SKQPTVSDAS GQRLGSSLSV ILENAKRHKK DMLDSYVSVE HFLLAYYSDT
201: RFGQEFFRDM KLDIQVLKDA IKDVRGDQRV TDRNPESKYQ ALEKYGNDLT EMARRGKLDP VIGRDDEIRR CIQILCRRTK NNPVIIGEPG VGKTAIAEGL
301: AQRIVRGDVP EPLMNRKLIS LDMGSLLAGA KFRGDFEERL KAVMKEVSAS NGQTILFIDE IHTVVGAGAM DGAMDASNLL KPMLGRGELR CIGATTLTEY
401: RKYIEKDPAL ERRFQQVLCV QPSVEDTISI LRGLRERYEL HHGVTISDSA LVSAAVLADR YITERFLPDK AIDLVDEAGA KLKMEITSKP TELDGIDRAV
501: IKLEMEKLSL KNDTDKASKE RLQKIENDLS TLKQKQKELN VQWEKEKSLM TKIRSFKEEI DRVNLEIESA EREYDLNRAA ELKYGTLLSL QRQLEEAEKN
601: LTNFRQFGQS LLREVVTDLD IAEIVSKWTG IPLSNLQQSE REKLVMLEEV LHHRVIGQDM AVKSVADAIR RSRAGLSDPN RPIASFMFMG PTGVGKTELA
701: KALAGYLFNT ENAIVRVDMS EYMEKHSVSR LVGAPPGYVG YEEGGQLTEV VRRRPYSVVL FDEIEKAHPD VFNILLQLLD DGRITDSQGR TVSFKNCVVI
801: MTSNIGSHHI LETLRNNEDS KEAVYEIMKR QVVELARQNF RPEFMNRIDE YIVFQPLDSN EISKIVELQM RRVKNSLEQK KIKLQYTKEA VDLLAQLGFD
901: PNYGARPVKR VIQQMVENEI AVGILKGDFA EEDTVLVDVD HLASDNKLVI KKLESNASAE EMAA

Arabidopsis Description

CLPB4Chaperone protein ClpB4, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q8VYJ7]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.