Factsheet for Bra008513.1-P

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 2
mitochondrion 2
nucleus 3
cytosol 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra007265.1-P
Bra014674.1-P
Bra014675.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G64860.1	Bra007265.1-P	AT3G56710.1	11943170
AT1G64860.1	Bra014674.1-P	AT3G56710.1	11943170
AT1G64860.1	Bra014675.1-P	AT3G56710.1	11943170

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY56127	Canola	nucleus	99.19	99.39
CDY52962	Canola	nucleus	96.54	98.96
AT1G64860.1	Thale cress	nucleus	87.4	85.66
KRH39837	Soybean	plastid	71.54	69.98
KRH23963	Soybean	plastid	71.54	69.98
Solyc03g097320.2.1	Tomato	nucleus, plastid	69.11	66.8
PGSC0003DMT400069555	Potato	nucleus	67.07	65.35
Solyc06g072710.2.1	Tomato	nucleus	66.87	65.15
GSMUA_Achr1P10700_001	Banana	nucleus	54.27	63.12
HORVU7Hr1G069520.2	Barley	nucleus	37.8	62.0
GSMUA_Achr11P...	Banana	nucleus	56.71	60.78
VIT_16s0039g00660.t01	Wine grape	plastid	69.92	59.52
Os08t0163400-01	Rice	nucleus	46.95	55.4
EES13412	Sorghum	plastid	51.83	51.0
Zm00001d049494_P001	Maize	plastid	51.22	50.0
PGSC0003DMT400068109	Potato	nucleus	61.99	50.0
Zm00001d024507_P001	Maize	plastid	45.12	49.33
TraesCS7D01G292000.1	Wheat	plastid	51.22	49.32
TraesCS7A01G297900.2	Wheat	plastid	50.81	49.02
TraesCS7B01G184800.1	Wheat	nucleus, plastid	50.61	48.73
Bra008807.1-P	Field mustard	plastid	20.53	24.88
Bra017213.1-P	Field mustard	plastid	23.78	21.95
Bra018617.1-P	Field mustard	plastid	25.0	21.47
Bra030732.1-P	Field mustard	plastid	24.39	21.05
Bra026466.1-P	Field mustard	nucleus	20.53	20.78
Bra009735.1-P	Field mustard	plastid	20.12	19.41
Bra014857.1-P	Field mustard	nucleus, plastid	16.67	14.99
Bra023430.1-P	Field mustard	plastid	19.92	13.48

Protein Annotations

Gene3D:1.10.10.10	Gene3D:1.10.601.10	MapMan:15.6.2.1	EnsemblPlantsGene:Bra008513	EnsemblPlants:Bra008513.1	EnsemblPlants:Bra008513.1-P
GO:GO:0001053	GO:GO:0003674	GO:GO:0003676	GO:GO:0003677	GO:GO:0003700	GO:GO:0005488
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006139	GO:GO:0006352
GO:GO:0006355	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009507	GO:GO:0009536
GO:GO:0009628	GO:GO:0009987	GO:GO:0015979	GO:GO:0016043	GO:GO:0016987	GO:GO:0071461
GO:GO:0071482	GO:GO:0080005	GO:GO:2000142	GO:GO:2001141	InterPro:IPR036388	UniProt:M4CWB6
PFAM:PF04539	PFAM:PF04542	PFAM:PF04545	PRINTS:PR00046	ScanProsite:PS00715	PANTHER:PTHR30603
PANTHER:PTHR30603:SF14	InterPro:RNA_pol_sigma-70_dom	InterPro:RNA_pol_sigma70	InterPro:RNA_pol_sigma70_r2	InterPro:RNA_pol_sigma70_r3	InterPro:RNA_pol_sigma70_r4
InterPro:RNA_pol_sigma_r2	InterPro:RNA_pol_sigma_r3/r4-like	SUPFAM:SSF88659	SUPFAM:SSF88946	TIGRFAMs:TIGR02937	UniParc:UPI0002546E33
InterPro:WH-like_DNA-bd_sf	SEG:seg	:	:	:	:

Description

AT1G64860 (E=4e-182) SIGA, SIG1, SIG2, SIGB, RPOD1 | SIGA (SIGMA FACTOR A); DNA binding / DNA-directed RNA polymerase/ sigma factor/ transcription factor

Coordinates

chrA02:-:16088493..16090741

Molecular Weight (calculated)

55735.8 Da

IEP (calculated)

9.941

GRAVY (calculated)

-0.510

Length

492 amino acids

Sequence
(BLAST)

001: MMATTAVVGL NAGKRLLSSS FYYSDVTEKF LSVNDLCSSQ YYYHIASTRS GITAKKTSSS SSSSNYSPSF HSSSRHTQSA KALKESVDVV QQWLPPGSDQ
101: ELEEESHEYE DDDTVEALLL LQRSLLEKQW NLSFEKKKKK ILVTCSGTSA RQRRMGAKKK TNIKVVNSDL FQNNRVKGGY VKGVISDDVL SHAEVVRLSK
201: KIKSGLRLDE HKSRLKDRLG CEPSDEQLAV SLKISRAELQ AWLMECNLAR EKLAMSNVRL VMSIAQRYDN MGAEMSDLVQ GGLIGLLRGI EKFDSSKGFR
301: ISTYVYWWIR QGVSRALVDN SRTLRLPTHL HERLGLIRNA KLRLQEKGVT PSIDRIAESL NMSQKKVRNA TEAVSKIFSL DRDAFPSLNG LPGETHHSYI
401: ADNRLENNPW HGYDEWALKD EVSKLISATL GEREREIIRL YYGLDKECLT WEDISKRIGL SRERVRQVGL VALEKLKHAA RKSKMEAMIL KN

Best Arabidopsis Sequence Match ( AT1G64860.1 )
(BLAST)

001: MATAAVIGLN TGKRLLSSSF YHSDVTEKFL SVNDHCSSQY HIASTKSGIT AKKASNYSPS FPSSNRHTQS AKALKESVDV ASTEKPWLPN GTDKELEEEC
101: YDDDDLISHS VEAILLLQKS MLEKSWNLSF EKAVSSEYPG KGTIRKKKIP VITCSGISAR QRRIGAKKKT NMTHVKAVSD VSSGKQVRGY VKGVISEDVL
201: SHVEVVRLSK KIKSGLRLDD HKSRLKDRLG CEPSDEQLAV SLKISRAELQ AWLMECHLAR EKLAMSNVRL VMSIAQRYDN LGAEMSDLVQ GGLIGLLRGI
301: EKFDSSKGFR ISTYVYWWIR QGVSRALVDN SRTLRLPTHL HERLGLIRNA KLRLQEKGIT PSIDRIAESL NMSQKKVRNA TEAVSKVFSL DRDAFPSLNG
401: LPGETHHSYI ADTRLENNPW HGYDDLALKE EVSKLISATL GEREKEIIRL YYGLDKECLT WEDISKRIGL SRERVRQVGL VALEKLKHAA RKRKMEAMIL
501: KN

Arabidopsis Description

SIGARNA polymerase sigma factor sigA [Source:UniProtKB/Swiss-Prot;Acc:O24629]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.