CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

extracellular 2
endoplasmic reticulum 2
vacuole 2
plasma membrane 2
golgi 2
plastid 2
peroxisome 1
mitochondrion 1
cytosol 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, peroxisome, plastid, secretory
BaCelLo:	secretory
EpiLoc:	plastid
iPSORT:	secretory
MultiLoc:	peroxisome
Plant-mPloc:	plastid
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161
extracellular: 22945350
endoplasmic reticulum: 27224218
mitochondrion: 27780359
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 22945350 doi

C Liao, F Hochholdinger, C Li

Key Laboratory of Plant-Soil Interactions, Ministry of Education, Center for Resources, Environment and Food Security, China Agricultural University, Beijing, China.

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH69119	Soybean	mitochondrion, nucleus	98.48	97.29
VIT_07s0005g03360.t01	Wine grape	extracellular	94.21	93.07
Solyc09g090140.2.1	Tomato	plastid	93.9	92.77
PGSC0003DMT400044217	Potato	cytosol	93.6	92.47
GSMUA_Achr4P21920_001	Banana	cytosol, endoplasmic reticulum, peroxisome	92.68	91.57
GSMUA_Achr5P03900_001	Banana	plasma membrane	91.77	90.66
EER94077	Sorghum	peroxisome	91.77	90.66
AT5G43330.1	Thale cress	cytosol, endoplasmic reticulum, peroxisome	90.55	89.46
Zm00001d032695_P001	Maize	plasma membrane	90.55	89.46
Os10t0478200-01	Rice	extracellular	90.55	89.46
CDY43609	Canola	peroxisome	90.55	89.46
CDY32953	Canola	peroxisome	90.24	89.16
Bra027485.1-P	Field mustard	peroxisome	90.24	89.16
HORVU1Hr1G041250.5	Barley	extracellular	90.24	88.89
TraesCS1D01G153900.1	Wheat	golgi, plastid	90.24	88.89
CDY18230	Canola	peroxisome	89.94	88.86
CDY15504	Canola	cytosol, endoplasmic reticulum, peroxisome	89.94	88.86
CDX89826	Canola	peroxisome	89.94	88.86
TraesCS1B01G172400.1	Wheat	golgi	89.94	88.59
CDX98109	Canola	cytosol, endoplasmic reticulum, peroxisome	59.15	88.58
AT1G04410.1	Thale cress	peroxisome	89.63	88.55
CDY67454	Canola	cytosol, endoplasmic reticulum, peroxisome	89.63	88.55
Bra030569.1-P	Field mustard	cytosol, golgi, peroxisome	89.33	88.25
TraesCS1A01G155200.1	Wheat	endoplasmic reticulum	87.8	87.8
Bra015314.1-P	Field mustard	peroxisome	88.72	87.65
Zm00001d014030_P001	Maize	cytosol, endoplasmic reticulum, peroxisome	82.93	87.18
VIT_07s0005g03350.t01	Wine grape	plastid	94.21	84.66
KRH19181	Soybean	endoplasmic reticulum	84.15	73.99
KRH34668	Soybean	plastid	41.16	30.96
KRG92120	Soybean	plastid	39.02	30.19

Protein Annotations

KEGG:00020+1.1.1.37	KEGG:00270+1.1.1.37	KEGG:00620+1.1.1.37	KEGG:00630+1.1.1.37	KEGG:00680+1.1.1.37	KEGG:00710+1.1.1.37
KEGG:00720+1.1.1.37	MapMan:1.4.2	EntrezGene:100811402	Gene3D:3.40.50.720	Gene3D:3.90.110.10	MapMan:5.1.1.3
EMBL:ACUP02006076	EnsemblPlantsGene:GLYMA_10G006500	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005975	GO:GO:0006091	GO:GO:0006099	GO:GO:0006107
GO:GO:0006108	GO:GO:0006139	GO:GO:0006734	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987
GO:GO:0016491	GO:GO:0016615	GO:GO:0016616	GO:GO:0019752	GO:GO:0030060	GO:GO:0055114
UniProt:H2D5S3	InterPro:IPR015955	EMBL:JN555608	EnsemblPlants:KRH31702	ProteinID:KRH31702	ProteinID:KRH31702.1
InterPro:L-lactate/malate_DH	InterPro:Lactate/malate_DH_C	InterPro:Lactate/malate_DH_N	InterPro:Lactate_DH/Glyco_Ohase_4_C	HAMAP:MF_01517	InterPro:Malate_DH_AS
InterPro:Malate_DH_NAD-dep_euk	InterPro:Malate_DH_type2	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00056	PFAM:PF02866	PIRSF:PIRSF000102
ScanProsite:PS00068	PANTHER:PTHR23382	PANTHER:PTHR23382:SF7	MetaCyc:PWY-1622	MetaCyc:PWY-5392	MetaCyc:PWY-561
MetaCyc:PWY-5690	MetaCyc:PWY-5913	MetaCyc:PWY-6728	MetaCyc:PWY-6969	MetaCyc:PWY-7115	MetaCyc:PWY-7383
SUPFAM:SSF51735	SUPFAM:SSF56327	TIGRFAMs:TIGR01758	TIGRFAMs:TIGR01759	UniParc:UPI000189DA3B	SEG:seg

Description

hypothetical protein

Coordinates

chr10:+:633423..637181

Molecular Weight (calculated)

35206.6 Da

IEP (calculated)

6.250

GRAVY (calculated)

0.078

Length

328 amino acids

Sequence
(BLAST)

001: MAKDPVRVLV TGAAGQIGYA LVPMIARGVM LGPDQPVILH MLDIPPAAES LNGVKMELVD AAFPLLKGVV ATTDVVEACT GVNIAVMVGG FPRKEGMERK
101: DVMTKNVSIY KSQASALEKH AAANCKVLVV ANPANTNALI LKEFAPSIPE KNISCLTRLD HNRALGQISE RLNIQVSDVK NVIIWGNHSS TQYPDVNHAT
201: VGEKPVRELI ADDAWLNGEF ITTVQQRGAA IIKARKLSSA LSAASAACDH IRDWVLGTPQ GTWVSMGVYS DGSYNVPAGL IYSFPVTCAN GEWTIVQGLP
301: IDEFSRKKLD LTAEELSEEK ALAYSCLN

Best Arabidopsis Sequence Match ( AT5G43330.1 )
(BLAST)

001: MAKEPVRVLV TGAAGQIGYA LVPMIARGIM LGADQPVILH MLDIPFAAEA LNGVKMELVD AAFPLLKGVV ATTDAVEACT GVNVAVMVGG FPRKEGMERK
101: DVMSKNVSIY KSQASALEKH AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNITCLTRLD HNRALGQVSE RLSVPVSDVK NVIIWGNHSS TQYPDVNHAT
201: VKTSVGEKPV RELVKNDEWL NGEFISTVQQ RGAAIIKARK LSSALSAASS ACDHIRDWVV GTPEGTFVSM GVYSDGSYNV PAGLIYSFPV TCRNGEWTIV
301: QGLPIDDASR KKMDLTAEEL KEEKDLAYSC LS

Arabidopsis Description

MDH2Malate dehydrogenase 2, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:P57106]
SUBAcon: [endoplasmic reticulum,cytosol,peroxisome]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.