CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, golgi

Predictor Summary:

plastid 2
plasma membrane 2
mitochondrion 1
extracellular 1
endoplasmic reticulum 1
vacuole 1
golgi 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	golgi, plasma membrane
Any Predictor:	mitochondrion, plastid, secretory
BaCelLo:	plastid
MultiLoc:	plasma membrane
PProwler:	mitochondrion
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	golgi

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH32102
KRH41747
KRH60449
KRH71329
KRH71332

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G09420.1	KRH41747	AT3G20000.1	17981999
AT5G09420.1	KRH60449	AT3G20000.1	17981999
AT5G09420.1	KRH32102	AT3G54660.1	17981999
AT5G09420.1	KRH71329	AT3G54660.1	17981999
AT5G09420.1	KRH71332	AT3G54660.1	17981999

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400042984	Potato	extracellular, plasma membrane	66.67	64.86
KRH10544	Soybean	golgi	73.15	39.63
KRH43896	Soybean	cytosol, endoplasmic reticulum	73.15	39.63
VIT_12s0028g00300.t01	Wine grape	cytosol	67.28	35.91
Solyc10g047300.1.1	Tomato	plastid	66.05	35.79
AT5G09420.1	Thale cress	cytosol	64.51	34.66
CDY21581	Canola	cytosol	64.51	34.66
KRG92836	Soybean	cytosol	44.75	33.49
KRH33958	Soybean	cytosol	44.44	33.26
KXG30729	Sorghum	plastid	57.1	30.13
GSMUA_Achr9P14860_001	Banana	mitochondrion	57.72	30.02
TraesCS6D01G298000.1	Wheat	plastid	55.86	29.72
TraesCS6B01G349000.1	Wheat	plastid	55.56	29.56
TraesCS6A01G318800.1	Wheat	plastid	55.56	29.56
Zm00001d018098_P005	Maize	plasma membrane	55.25	29.15
Os02t0754500-01	Rice	cytosol, mitochondrion, plastid	54.63	28.87
KRH41841	Soybean	endoplasmic reticulum	48.77	26.73
KRH60539	Soybean	extracellular	47.53	26.24
Bra009383.1-P	Field mustard	cytosol, plastid	63.89	22.43
HORVU6Hr1G078210.1	Barley	mitochondrion	36.73	21.33
KRG98874	Soybean	mitochondrion	20.68	14.11
KRH10653	Soybean	cytosol	24.69	13.18
KRH59629	Soybean	golgi	24.69	13.16
KRH40976	Soybean	golgi	24.69	13.14
KRH46093	Soybean	mitochondrion	21.91	13.08
KRH21697	Soybean	cytosol	24.38	13.01
KRH63971	Soybean	cytosol, golgi, peroxisome	22.84	11.94
KRH53966	Soybean	cytosol, golgi, peroxisome	22.84	11.92
KRH41039	Soybean	mitochondrion	16.36	10.86
KRH59711	Soybean	plasma membrane	20.06	10.5
KRH58262	Soybean	cytosol	0.0	0.0

Protein Annotations

KEGG:00970+6.3.5.7	Gene3D:3.90.1300.10	MapMan:35.1	UniProt:A0A0R0JZC6	UniProt:A0A2K7KME1	EMBL:ACUP02003244
EMBL:ACUP02003245	InterPro:AS_sf	InterPro:Amidase	InterPro:Amidase_dom	EnsemblPlantsGene:GLYMA_05G1167001	GO:GO:0003674
GO:GO:0003824	GO:GO:0004040	GO:GO:0005575	GO:GO:0016020	GO:GO:0016021	GO:GO:0016787
InterPro:IPR036928	EnsemblPlants:KRH58264	ProteinID:KRH58264	ProteinID:KRH58264.1	ProteinID:KRH58265.1	PFAM:PF01425
PANTHER:PTHR11895	PANTHER:PTHR11895:SF92	MetaCyc:PWY-5921	SUPFAM:SSF75304	TMHMM:TMhelix	UniParc:UPI0006EDBF0D
UniParc:UPI000C871BC8	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr5:-:30945986..30951126

Molecular Weight (calculated)

35175.4 Da

IEP (calculated)

9.756

GRAVY (calculated)

-0.019

Length

324 amino acids

Sequence
(BLAST)

001: MSQSLKLIKE HASNPKLWLV IGIGVAGIVV LVETRRRTRR GKTHKQDFGA FVERFELLPF PQPPPPAAKQ SLSALTFAIN DTFDVKDYVT GFGNSTWKST
101: HKAAEKTAVV VTALLMSGAT CVGKTVVDEF SFGISGENKY YGTPTHPQMP SCKLGGSSCG SAVAVAAGLV DFAVGTDTTG CVRIPASFCG IFGFRPSHGA
201: VSTIGVLPNA QSLDTIGWFA RDPSILHRVG HVLLQLNSVE TKRSRHFIFA DDLFQLSKIP TQNTIYVIGK AIENMSGYQA PKHLNLCQYI DSRVPSLRLH
301: QQSTHQQNET SILKTLSSVM LSLQ

Best Arabidopsis Sequence Match ( AT1G08980.1 )
(BLAST)

001: MATNNDFGAF IEKVTISPTS TSSSPPSLQG LTFAIKDIFD VEGRVTGFGN PDWLRTHSAA TSTAPVVSSL LEAGATALGI TIMDEMAYSI NGENAHYGTP
101: RNPIAFDRVP GGSSSGSAVA VAARLVDFSI GTDTGGSVRV PASYCGIFGF RPSHGAVSTV GLTPMAQSFD TVGWFARDTA TLKRVGCVLL QQHHLNPIEP
201: SQLIIADDCF KLCSVPHDLL VQPLVGSVEK SFGGNTVVKK VNLGEYIGQN VPSLKHFMTS DDVTTQQEFC IPSLMALSSS MRLLQRHEFK INHGAWISSV
301: KPEFGPGISE RIEEAIRTSD EKIDHCRSVK SELITALSTL LGEKGVLVIP TVPGPPPHLQ ANVAALESFR SRAFSLLSIA GVSGFCQVSI PLGLHENLPV
401: SVSLVAKYGS DGFLLSLVDS LAAFI

Arabidopsis Description

AMI1Amidase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9FR37]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.