Factsheet for Os03t0243300-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 4
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plasma membrane: 28056797

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0179300-01
Os03t0131300-01
Os10t0542200-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G38630.1	Os02t0179300-01	AT1G16190.1	20059542
AT4G38630.1	Os03t0131300-01	AT2G17200.1	20059542
AT4G38630.1	Os10t0542200-01	AT2G17200.1	20059542

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER92569	Sorghum	cytosol	90.3	90.52
TraesCS4D01G227900.2	Wheat	cytosol	88.81	89.03
TraesCS4A01G071300.2	Wheat	cytosol	88.31	88.53
TraesCS4B01G227200.2	Wheat	cytosol	88.81	88.37
Zm00001d028396_P001	Maize	nucleus	83.33	74.11
Zm00001d047941_P001	Maize	cytosol	82.84	74.0
PGSC0003DMT400009215	Potato	cytosol	72.64	72.46
GSMUA_Achr4P25580_001	Banana	cytosol	71.64	72.0
Solyc02g083710.2.1	Tomato	endoplasmic reticulum, extracellular	72.14	71.96
HORVU4Hr1G063820.1	Barley	cytosol, mitochondrion, nucleus	87.81	71.75
KRH69766	Soybean	cytosol	71.64	71.11
KRH76642	Soybean	cytosol	71.64	71.11
KRH28809	Soybean	nucleus	71.64	71.11
KRH08049	Soybean	cytosol	69.15	69.85
GSMUA_Achr2P11290_001	Banana	cytosol, nucleus, plastid	66.17	68.03
AT4G38630.1	Thale cress	cytosol	63.93	66.58
Bra010735.1-P	Field mustard	cytosol	63.18	66.32
Bra033573.1-P	Field mustard	cytosol	62.69	66.14
CDY22415	Canola	cytosol	61.44	65.87
CDX90286	Canola	cytosol, nucleus, plastid	61.69	65.78
CDX72742	Canola	extracellular	60.95	65.68
CDY12093	Canola	cytosol	61.44	65.52
GSMUA_Achr11P...	Banana	golgi, vacuole	72.14	62.77
Zm00001d024336_P001	Maize	mitochondrion	14.18	60.0
PGSC0003DMT400058116	Potato	cytosol	53.48	50.47
Os10t0141400-00	Rice	cytosol	39.8	49.38
Solyc03g033430.1.1	Tomato	cytosol	19.15	45.29
Zm00001d040834_P001	Maize	plastid	21.89	44.22
VIT_04s0008g00060.t01	Wine grape	nucleus	9.95	38.84

Protein Annotations

MapMan:19.2.6.2.2.9	Gene3D:3.40.50.410	EntrezGene:4332222	EMBL:AB010740	EMBL:AB037151	ProteinID:ABF94915.1
ProteinID:ABF94916.1	EMBL:AK073601	ProteinID:BAF11441.1	ProteinID:BAS83212.1	ncoils:Coil	ProteinID:EEE58678.1
GO:GO:0000502	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005634	GO:GO:0005737	GO:GO:0005829	GO:GO:0008150	GO:GO:0008152
GO:GO:0008540	GO:GO:0009056	GO:GO:0009987	GO:GO:0016043	GO:GO:0019538	GO:GO:0031593
GO:GO:0043161	GO:GO:0043248	InterPro:IPR002035	InterPro:IPR003903	InterPro:IPR036465	EMBL:JX972162
UniProt:O82143	EnsemblPlantsGene:Os03g0243300	EnsemblPlants:Os03t0243300-01	PFAM:PF02809	PFAM:PF13519	PFscan:PS50234
PFscan:PS50330	PANTHER:PTHR10223	PANTHER:PTHR10223:SF5	SMART:SM00327	SMART:SM00726	SUPFAM:SSF53300
InterPro:UIM_dom	UniParc:UPI00000A0025	InterPro:VWF_A	RefSeq:XP_015632315.1	SEG:seg	InterPro:vWFA_dom_sf

Description

Subunit 5 a, 26S proteasome subunit 5a, 19S regulatory particle non-ATPase subunit 10, RP non-ATPase 10, Regulatory Particle Non-ATPase 10Similar to 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome regulatory subunit S5A) (Multiubiquitin chain binding protein). (Os03t0243300-01)

Coordinates

chr3:-:7579448..7583738

Molecular Weight (calculated)

42385.4 Da

IEP (calculated)

4.248

GRAVY (calculated)

-0.392

Length

402 amino acids

Sequence
(BLAST)

001: MVLEATMICI DNSEWMRNGD YSPSRFQAQA DAVNLICGAK TQSNPENTVG VMTMAGKGVR VLVTPTSDLG KILACMHGLE VGAEANLAAA IQVAQLALKH
101: RQNKRQQQRI IAFIGSPVKY DKKVLETIGK KLKKNNVALD IVDFGETDDD KPEKLEALIS AVNSSDSSHI VHVPPGENAL SDVLISTPIF TGEEGGSGFA
201: ASAAAAAATG AAGFEFDVDP NVDPELALAL RLSMEEERAR QEAIAKKAAE ESSGAENKDH ASSSNADSVM AEAEPASNAA DDKKDQPKED DDAQLLQQAL
301: AMSMEEGSSG AAAADAAMAE AAVDDQDLAL ALQMSVQDAG GSSQSDMSKV FEDRSFVTSI LNSLPGVDPN DPSVKDLLAS LHGQGEQEKK EDKSDKPEDE
401: KK

Best Arabidopsis Sequence Match ( AT4G38630.1 )
(BLAST)

001: MVLEATMICI DNSEWMRNGD YSPSRLQAQT EAVNLLCGAK TQSNPENTVG ILTMAGKGVR VLTTPTSDLG KILACMHGLD VGGEINLTAA IQIAQLALKH
101: RQNKNQRQRI IVFAGSPIKY EKKALEIVGK RLKKNSVSLD IVNFGEDDDE EKPQKLEALL TAVNNNDGSH IVHVPSGANA LSDVLLSTPV FTGDEGASGY
201: VSAAAAAAAA GGDFDFGVDP NIDPELALAL RVSMEEERAR QEAAAKKAAD EAGQKDKDGD TASASQETVA RTTDKNAEPM DEDSALLDQA IAMSVGDVNM
301: SEAADEDQDL ALALQMSMSG EESSEATGAG NNLLGNQAFI SSVLSSLPGV DPNDPAVKEL LASLPDESKR TEEEESSSKK GEDEKK

Arabidopsis Description

RPN10RPN10 [Source:UniProtKB/TrEMBL;Acc:A0A178V4I4]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.