Factsheet for Os04t0446300-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid

Predictor Summary:

plastid 3
golgi 2
endoplasmic reticulum 2
extracellular 1
vacuole 1
plasma membrane 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane, plastid
Any Predictor:	plastid, secretory
BaCelLo:	plastid
iPSORT:	plastid
MultiLoc:	golgi
Plant-mPloc:	endoplasmic reticulum
PProwler:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plastid

plasma membrane: 18260611
plastid: 27992503

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0509600-01
Os05t0297900-01
Os06t0273800-02

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G39960.1	Os02t0509600-01	AT1G27300.1	21798944
AT2G39960.1	Os05t0297900-01	AT3G15710.1	21798944
AT2G39960.1	Os06t0273800-02	AT3G15710.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
OQU81657	Sorghum	plasma membrane	92.71	92.71
TraesCS2A01G314500.1	Wheat	golgi	89.06	89.06
TraesCS2D01G312700.1	Wheat	plasma membrane	88.54	88.54
TraesCS2B01G332900.1	Wheat	golgi	88.02	88.02
HORVU2Hr1G078290.3	Barley	golgi, mitochondrion, plastid	89.58	79.63
GSMUA_Achr7P18540_001	Banana	golgi, peroxisome, plasma membrane	71.88	74.59
GSMUA_Achr5P23170_001	Banana	golgi, peroxisome, plastid	70.31	74.59
Os02t0565200-01	Rice	golgi, plasma membrane, plastid	72.92	73.3
KRH14989	Soybean	endoplasmic reticulum, mitochondrion	68.75	71.74
CDY07042	Canola	cytosol	36.98	71.0
KRH73149	Soybean	endoplasmic reticulum	67.71	70.65
CDX93221	Canola	cytosol	36.46	70.0
CDY20503	Canola	endoplasmic reticulum	41.15	67.52
Solyc04g072110.2.1	Tomato	cytosol	20.83	66.67
PGSC0003DMT400064539	Potato	golgi	66.15	66.49
Zm00001d020624_P002	Maize	plastid	23.96	64.79
AT2G39960.1	Thale cress	cytosol, golgi, plasma membrane	63.54	63.54
Bra004998.1-P	Field mustard	cytosol	64.06	62.12
VIT_13s0019g03400.t01	Wine grape	plasma membrane	63.02	62.05
CDY60993	Canola	cytosol, endoplasmic reticulum, plasma membrane	60.94	60.62
CDY24940	Canola	cytosol	59.38	59.38
CDY24935	Canola	cytosol	59.38	59.38
CDX74817	Canola	cytosol	64.58	59.33
Zm00001d013600_P001	Maize	cytosol	22.92	58.67
HORVU4Hr1G058460.1	Barley	golgi	29.69	55.88
AT4G04200.1	Thale cress	cytosol, golgi, plasma membrane	55.21	55.79
CDX93220	Canola	cytosol	25.0	55.17
CDY07043	Canola	cytosol	27.6	51.46
CDY20504	Canola	cytosol	25.0	48.98
Bra017043.1-P	Field mustard	cytosol	50.0	44.24

Protein Annotations

MapMan:18.10.1.1.3	EntrezGene:4335967	EMBL:AK059953	EMBL:AK071742	EMBL:AK099024	ProteinID:BAF14823.1
ProteinID:BAS89401.1	ProteinID:CAD40959.2	ProteinID:EEE61077.1	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005783	GO:GO:0005787	GO:GO:0006465
GO:GO:0006810	GO:GO:0008150	GO:GO:0008152	GO:GO:0008233	GO:GO:0009987	GO:GO:0016020
GO:GO:0016021	GO:GO:0016787	GO:GO:0019538	GO:GO:0045047	EnsemblPlantsGene:Os04g0446300	EnsemblPlants:Os04t0446300-01
PFAM:PF06703	PANTHER:PTHR13085	UniProt:Q7XUX4	InterPro:SigPept_cplx_su2	TMHMM:TMhelix	UniParc:UPI000021C938
RefSeq:XP_015633680.1	SEG:seg	:	:	:	:

Description

Microsomal signal peptidase 25 kDa subunit family protein. (Os04t0446300-01)

Coordinates

chr4:-:22246672..22249716

Molecular Weight (calculated)

21130.6 Da

IEP (calculated)

9.703

GRAVY (calculated)

-0.017

Length

192 amino acids

Sequence
(BLAST)

001: MASDGAAATP AKASPKKANL LDPHSIKHLL DETISDVVKS KGYAEDTRLG NWKLAIGAAV IAIALLAQFY PKKFPQNREF LLGCIALYVV LNVVLLILSY
101: TKEKDAIMFT HPPAGSFNST GLVISSKLPR FSDMYTITIA SADPQSISAN KPVHFTKSVT KWFTKEGVLV EGLFWKDVER LIDDYNTERK GK

Best Arabidopsis Sequence Match ( AT2G39960.1 )
(BLAST)

001: MEEKKTESTN KNVKKANLLD HHSIKHILDE SVSDIVTSRG YKEDVRLSNL KLILGTIIIV VALVAQFYNK KFPENRDFLI GCIALYVVLN AVLQLILYTK
101: EKNAILFTYP PEGSFTSTGL VVSSKLPRFS DQYTLTIDSA DPKSISAGKS VQLTKSVTQW FTKDGVLVEG LFWKDVEALI KNYAEEEPKK KK

Arabidopsis Description

Probable signal peptidase complex subunit 2 [Source:UniProtKB/Swiss-Prot;Acc:P58684]
SUBAcon: [plasma membrane,golgi,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.