Factsheet for Os09t0110400-02

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 5
mitochondrion 3

Predictors

GFP

MS/MS

Papers

nucleus: 19621931
cytosol: 19685898
nucleus: 23777608
plasma membrane: 27800704

msms PMID: 23777608 doi

H Mujahid, F Tan, J Zhang, BR Nallamilli, K Pendarvis, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 19685898 doi

KA Doroshenk, AJ Crofts, RT Morris, JJ Wyrick, TW Okita

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.

msms PMID: 19621931 doi

T Aki, S Yanagisawa

Department of Applied Biological Chemistry, The University of Tokyo, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os05t0365500-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G15400.1	Os05t0365500-01	AT1G10270.1	16489121

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER98576	Sorghum	nucleus	88.18	88.72
TraesCS7A01G519700.1	Wheat	mitochondrion, nucleus	84.55	88.29
TraesCS5B01G158300.1	Wheat	nucleus	88.18	87.92
TraesCS7B01G435800.1	Wheat	mitochondrion, nucleus	83.64	87.62
TraesCS7D01G509400.1	Wheat	nucleus	83.33	87.3
TraesCS5A01G160900.1	Wheat	plastid	87.27	87.01
Zm00001d005295_P001	Maize	extracellular	87.58	82.57
HORVU7Hr1G115770.2	Barley	nucleus	41.82	81.66
TraesCS5D01G165800.2	Wheat	mitochondrion, nucleus	87.27	79.34
HORVU5Hr1G050250.6	Barley	nucleus	86.67	77.51
VIT_03s0180g00050.t01	Wine grape	nucleus	74.24	77.04
KRH50412	Soybean	nucleus	73.64	76.18
KRG89365	Soybean	nucleus	73.64	75.94
GSMUA_Achr1P26120_001	Banana	mitochondrion, nucleus	75.15	75.15
Solyc05g010300.2.1	Tomato	nucleus	71.52	73.98
CDY62312	Canola	nucleus	71.21	73.9
CDY37227	Canola	nucleus	71.52	73.52
Bra039839.1-P	Field mustard	nucleus	71.52	73.52
Bra015218.1-P	Field mustard	nucleus	70.91	73.35
CDY36631	Canola	nucleus	70.91	73.35
AT2G15430.1	Thale cress	nucleus	70.3	72.73
CDX71219	Canola	nucleus	61.21	71.63
AT2G15400.1	Thale cress	nucleus	66.36	68.65
GSMUA_Achr3P13120_001	Banana	cytosol	26.67	62.86
CDX71220	Canola	nucleus	63.33	47.07
GSMUA_Achr3P13130_001	Banana	cytosol	16.06	46.09
Os03t0295400-01	Rice	nucleus	26.36	22.96
Os02t0723600-00	Rice	cytosol	17.58	19.33

Protein Annotations

KEGG:00230+2.7.7.6	KEGG:00240+2.7.7.6	MapMan:15.1.6.1	Gene3D:2.170.120.12	Gene3D:3.30.1360.270	EntrezGene:4346392
EMBL:AK061402	EMBL:AK068844	EMBL:AK121793	ProteinID:BAD17541.1	ProteinID:BAF24486.1	ProteinID:BAT06824.1
InterPro:DNA-dir_RNA_pol_30-40kDasu_CS	InterPro:DNA-dir_RNA_pol_RpoA/D/Rpb3	InterPro:DNA-dir_RNA_pol_insert	ProteinID:EEE69188.1	GO:GO:0001055	GO:GO:0003674
GO:GO:0003676	GO:GO:0003677	GO:GO:0003824	GO:GO:0003899	GO:GO:0005488	GO:GO:0005515
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005654	GO:GO:0005665
GO:GO:0006139	GO:GO:0006351	GO:GO:0006366	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058
GO:GO:0009987	GO:GO:0016740	GO:GO:0046983	InterPro:IPR036603	InterPro:IPR036643	EnsemblPlantsGene:Os09g0110400
EnsemblPlants:Os09t0110400-02	PFAM:PF01000	PFAM:PF01193	ScanProsite:PS00446	PANTHER:PTHR11800	PANTHER:PTHR11800:SF2
UniProt:Q6YX88	InterPro:RBP11-like	InterPro:RNApol_insert_sf	SMART:SM00662	SUPFAM:SSF55257	SUPFAM:SSF56553
UniParc:UPI000035AF36	RefSeq:XP_015612385.1	SEG:seg	:	:	:

Description

Similar to DNA-directed RNA polymerase II 36 kDa polypeptide A (EC 2.7.7.6) (RNA polymerase II subunit 3). (Os09t0110400-01);Similar to DNA-directed RNA polymerase II 36 kDa polypeptide A (EC 2.7.7.6) (RNA polymerase II subunit 3). (Os09t0110400-02);Similar to DNA-directed RNA polymerase II 36 kDa polypeptide A (EC 2.7.7.6) (RNA polymerase II subunit 3). (Os09t0110400-03)

Coordinates

chr9:+:927110..934049

Molecular Weight (calculated)

36462.2 Da

IEP (calculated)

4.494

GRAVY (calculated)

-0.255

Length

330 amino acids

Sequence
(BLAST)

001: MERAAGGVSY QRFPRVRIRE LKDDYAKFEL RDTDASMANA LRRVMIAEVP TVAIDLVEIE VNSSVLNDEF IAHRLGLIPL TSAAAMAMRF SRDCDACDGD
101: GSCEYCSVEF HLAARATDSD QTLEVTSNDL RSTDPKVCPV DQARAYQHAL GGTEPFDTAA AADQRGILIV KLRRGQELRL RAIARKGIGK DHAKWSPAAT
201: VTFMYEPEIR INEELMETLT LEEKRNLVES SPTKVFNIDP NTQQVVVEDA EAYTYDDEVI KKADAMGKPG LIEINAKEDS FIFTVETTGA ITAYELIMNA
301: ITVLRQKLDA VRLQDDDADL GELGAHLVGG

Best Arabidopsis Sequence Match ( AT2G15430.1 )
(BLAST)

001: MDGATYQRFP KIKIRELKDD YAKFELRETD VSMANALRRV MISEVPTVAI DLVEIEVNSS VLNDEFIAHR LGLIPLTSER AMSMRFSRDC DACDGDGQCE
101: FCSVEFRLSS KCVTDQTLDV TSRDLYSADP TVTPVDFTID SSVSDSSEHK GIIIVKLRRG QELKLRAIAR KGIGKDHAKW SPAATVTFMY EPDIIINEDM
201: MDTLSDEEKI DLIESSPTKV FGMDPVTRQV VVVDPEAYTY DEEVIKKAEA MGKPGLIEIS PKDDSFIFTV ESTGAVKASQ LVLNAIDLLK QKLDAVRLSD
301: DTVEADDQFG ELGAHMRGG

Arabidopsis Description

NRPB3RPB35.5A [Source:UniProtKB/TrEMBL;Acc:A0A178W202]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.