Factsheet for Os10t0580300-01

Subcellular Localization

min:

: max

Winner_takes_all: cytosol, plasma membrane

Predictor Summary:

nucleus 2
cytosol 4
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os03t0203800-01	nucleus
Os03t0617500-01	nucleus
Os06t0236600-00	endoplasmic reticulum
Os08t0544800-01
Os09t0111100-01	nucleus, nucleus
Os09t0382300-00	endoplasmic reticulum
Os09t0521300-00
Os12t0502300-01	nucleus
Os12t0588800-01	nucleus

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G63610.1	Os12t0502300-01	AT1G15570.1	20407024
AT5G63610.1	Os06t0236600-00	AT1G70210.1	20407024
AT5G63610.1	Os09t0382300-00	AT1G70210.1	20407024
AT5G63610.1	Os09t0111100-01	AT3G50070.1	20407024
AT5G63610.1	Os09t0111100-01	AT4G34160.1	20407024
AT5G63610.1	Os03t0203800-01	AT4G37630.1	20407024
AT5G63610.1	Os03t0617500-01	AT4G37630.1	20407024
AT5G63610.1	Os12t0588800-01	AT4G37630.1	20407024
AT5G63610.1	Os08t0544800-01	AT5G51910.1	21798944
AT5G63610.1	Os09t0521300-00	AT5G51910.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS1D01G201600.1	Wheat	cytosol	92.63	93.62
TraesCS1A01G198100.2	Wheat	cytosol	92.63	93.62
HORVU1Hr1G051380.3	Barley	cytosol	92.42	93.4
TraesCS1B01G212800.1	Wheat	cytosol	92.42	93.4
OQU92050	Sorghum	cytosol	90.95	91.72
Zm00001d014438_P001	Maize	cytosol	89.89	90.66
KRH59626	Soybean	cytosol	55.58	85.16
Solyc12g027870.1.1	Tomato	cytosol	60.0	84.82
GSMUA_Achr11P...	Banana	cytosol	82.11	83.69
GSMUA_Achr5P18030_001	Banana	cytosol	80.21	81.58
KRH40974	Soybean	cytosol, nucleus	75.79	78.09
KRH63972	Soybean	cytosol	75.37	77.49
VIT_07s0031g02680.t01	Wine grape	cytosol	78.11	77.13
AT5G63610.1	Thale cress	cytosol	73.68	74.47
CDY60036	Canola	cytosol	73.05	74.15
Bra037748.1-P	Field mustard	cytosol	72.84	73.93
CDX81189	Canola	cytosol, nucleus, plastid	67.37	73.56
KRH57887	Soybean	cytosol	35.16	70.76
Os01t0897000-01	Rice	cytosol	26.53	41.58
Os08t0512600-01	Rice	cytosol, plasma membrane	27.16	39.57
Os02t0123100-01	Rice	mitochondrion	29.26	36.97
Os03t0847600-01	Rice	cytosol	26.53	29.1
Os02t0700600-01	Rice	plasma membrane	25.68	26.58
Os04t0488000-01	Rice	nucleus	32.0	21.41
Os02t0602100-02	Rice	nucleus	30.53	20.92
Os02t0602400-01	Rice	cytosol	0.0	0.0

Protein Annotations

Gene3D:1.10.510.10	MapMan:13.1.1.2.5	MapMan:15.3.3.4.3	MapMan:18.4.3.1.5	Gene3D:3.30.200.20	EntrezGene:4349519
ProteinID:AAG46164.1	ProteinID:ABB48043.2	EMBL:AK066824	ProteinID:BAF27349.1	ProteinID:BAT12249.1	ProteinID:EEE51472.1
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0004693
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005654	GO:GO:0006464	GO:GO:0006468	GO:GO:0007049	GO:GO:0007346	GO:GO:0008150
GO:GO:0008152	GO:GO:0008353	GO:GO:0009987	GO:GO:0016301	GO:GO:0016310	GO:GO:0016592
GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:Kinase-like_dom_sf	EnsemblPlantsGene:Os10g0580300	EnsemblPlants:Os10t0580300-01
PFAM:PF00069	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24056	PANTHER:PTHR24056:SF92	InterPro:Prot_kinase_dom
UniProt:Q336M2	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI00000A46FE	RefSeq:XP_015614383.1
SEG:seg	:	:	:	:	:

Description

CYCLIN-DEPENDENT KINASE E;1Serine/threonine protein kinase domain containing protein. (Os10t0580300-01)

Coordinates

chr10:-:23148893..23153234

Molecular Weight (calculated)

52810.9 Da

IEP (calculated)

9.787

GRAVY (calculated)

-0.396

Length

475 amino acids

Sequence
(BLAST)

001: MGDGRVGGGT NRPAWLQQYE LVGKIGEGTY GLVFLARLKQ SHPHAAAGVG RRGSPIAIKK FKQSKEGDGV SPTAIREIML LREINHENVV KLVNVHINHA
101: DMSLYLAFDY AEHDLYEIIR HHREKLNLPI NPYTVKSLLW QLLNGLNYLH SNWIIHRDLK PSNILVMGEG EEHGIIKIAD FGLARIYQAP LKPLSDNGVV
201: VTIWYRAPEL LLGAKHYTSA VDMWAVGCIF AELLTLKPLF QGVEAKATPN PFQLDQLDKI FKVLGHPTVE KWPTLANLPC WQNDQQHIQG HKYENTGLHN
301: IVHLPQKSPA FDLLSKMLEY DPRKRITAAQ ALEHEYFRMD PLPGRNALLP SQAGEKIVQY PVRPVDTTTD FEGTTSLQPT QAPSGNAAPG NQSVVPRPIP
401: RQMQQPMVGM SRMGGTNMAA FGAAPQGGIA GMNPGNIPMQ RGAGAQSHPH QLRRKADQGM GMQNPGYPTQ QKRRF

Best Arabidopsis Sequence Match ( AT5G63610.1 )
(BLAST)

001: MGDGSSSRSN SSNSTSEKPE WLQQYNLVGK IGEGTYGLVF LARTKTPPKR PIAIKKFKQS KDGDGVSPTA IREIMLLREI SHENVVKLVN VHINFADMSL
101: YLAFDYAEYD LYEIIRHHRD KVGHSLNTYT VKSLLWQLLN GLNYLHSNWI IHRDLKPSNI LVMGDAEEHG IVKIADFGLA RIYQAPLKPL SDNGVVVTIW
201: YRAPELLLGS KHYTSAVDMW AVGCIFAELL TLKPLFQGAE AKSSQNPFQL DQLDKIFKIL GHPTMDKWPT LVNLPHWQND VQHIQAHKYD SVGLHNVVHL
301: NQKSPAYDLL SKMLEYDPLK RITASQALEH EYFRMDPLPG RNAFVASQPM EKNVNYPTRP VDTNTDFEGT TSINPPQAVA AGNVAGNMAG AHGMGSRSMP
401: RPMVAHNMQR MQQSQGMMAY NFPAQAGLNP SVPLQQQRGM AQPHQQQQLR RKDPGMGMSG YAPPNKSRRL

Arabidopsis Description

CDKE-1Cyclin-dependent kinase E-1 [Source:UniProtKB/Swiss-Prot;Acc:Q84TI6]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.