CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

nucleus 2
cytosol 4
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG90996	nucleus
KRG91429
KRG99255	endoplasmic reticulum
KRG99448	nucleus
KRH04529	nucleus
KRH04631	nucleus
KRH05060	nucleus
KRH16044	nucleus
KRH28363	nucleus
KRH28424	nucleus
KRH28480	nucleus
KRH35393
KRH35760	nucleus
KRH44391	nucleus
KRH45568	nucleus
KRH45743	endoplasmic reticulum
KRH47513	nucleus
KRH50258	nucleus
KRH52524	nucleus
KRH57804	nucleus
KRH57973	nucleus
KRH61850	nucleus
KRH69476	endoplasmic reticulum
KRH69654	nucleus
KRH71159	nucleus
KRH74510	nucleus
KRH74675	endoplasmic reticulum
KRH76992	nucleus
KRH77050	nucleus
KRH77118	nucleus

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G63610.1	KRH44391	AT1G15570.1	20407024
AT5G63610.1	KRH47513	AT1G15570.1	20407024
AT5G63610.1	KRG99255	AT1G70210.1	20407024
AT5G63610.1	KRH45743	AT1G70210.1	20407024
AT5G63610.1	KRH69476	AT1G70210.1	20407024
AT5G63610.1	KRH74675	AT1G70210.1	20407024
AT5G63610.1	KRH04631	AT3G19150.1	20407024
AT5G63610.1	KRH28480	AT3G19150.1	20407024
AT5G63610.1	KRH50258	AT3G19150.1	20407024
AT5G63610.1	KRH57804	AT3G19150.1	20407024
AT5G63610.1	KRH71159	AT3G19150.1	20407024
AT5G63610.1	KRH76992	AT3G19150.1	20407024
AT5G63610.1	KRH04529	AT3G50070.1	20407024
AT5G63610.1	KRH28424	AT3G50070.1	20407024
AT5G63610.1	KRH57973	AT3G50070.1	20407024
AT5G63610.1	KRH77050	AT3G50070.1	20407024
AT5G63610.1	KRG90996	AT4G34160.1	20407024
AT5G63610.1	KRH35760	AT4G34160.1	20407024
AT5G63610.1	KRH28363	AT4G37630.1	20407024
AT5G63610.1	KRH77118	AT4G37630.1	20407024
AT5G63610.1	KRG99448	AT5G10440.1	20407024
AT5G63610.1	KRH45568	AT5G10440.1	20407024
AT5G63610.1	KRH69654	AT5G10440.1	20407024
AT5G63610.1	KRH74510	AT5G10440.1	20407024
AT5G63610.1	KRH05060	AT5G11300.1	20407024
AT5G63610.1	KRH16044	AT5G11300.1	20407024
AT5G63610.1	KRH52524	AT5G11300.1	20407024
AT5G63610.1	KRH61850	AT5G11300.1	20407024
AT5G63610.1	KRG91429	AT5G51910.1	21798944
AT5G63610.1	KRH35393	AT5G51910.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH59626	Soybean	cytosol	66.16	98.39
KRH63972	Soybean	cytosol	87.2	87.01
Solyc12g027870.1.1	Tomato	cytosol	63.12	86.61
VIT_07s0031g02680.t01	Wine grape	cytosol	82.65	79.21
KRH57887	Soybean	cytosol	40.13	78.39
GSMUA_Achr11P...	Banana	cytosol	77.87	77.04
OQU92050	Sorghum	cytosol	78.09	76.43
TraesCS1D01G201600.1	Wheat	cytosol	77.87	76.38
TraesCS1A01G198100.2	Wheat	cytosol	77.87	76.38
TraesCS1B01G212800.1	Wheat	cytosol	77.66	76.17
HORVU1Hr1G051380.3	Barley	cytosol	77.66	76.17
GSMUA_Achr5P18030_001	Banana	cytosol	76.79	75.8
Os10t0580300-01	Rice	cytosol, plasma membrane	78.09	75.79
Bra037748.1-P	Field mustard	cytosol	76.79	75.64
CDY60036	Canola	cytosol	76.57	75.43
AT5G63610.1	Thale cress	cytosol	76.79	75.32
Zm00001d014438_P001	Maize	cytosol	76.79	75.16
CDX81189	Canola	cytosol, nucleus, plastid	70.72	74.94
KRH36887	Soybean	cytosol, nucleus	29.07	45.58
KRH11866	Soybean	cytosol, nucleus	29.07	45.58
KRH42252	Soybean	cytosol	28.63	44.9
KRH58370	Soybean	cytosol	28.85	44.33
KRH47306	Soybean	cytosol, nucleus, plasma membrane	27.77	40.76
KRH48128	Soybean	cytosol	27.77	40.63
KRH37522	Soybean	cytosol	27.77	40.38
KRH06014	Soybean	cytosol	26.9	39.49
KRH41782	Soybean	cytosol, nucleus	29.72	37.74
KRH57208	Soybean	cytosol	17.14	37.26
KRH17547	Soybean	cytosol	27.11	37.09
KRH13011	Soybean	cytosol	24.73	34.65
KRH60482	Soybean	cytosol	30.59	34.47
KRH26046	Soybean	cytosol	21.91	34.24
KRH39114	Soybean	nucleus	29.07	32.6
KRH44859	Soybean	cytosol	23.43	32.34
KRH48600	Soybean	cytosol	27.77	31.92
KRH66138	Soybean	cytosol	26.25	27.82
KRH07433	Soybean	cytosol	26.03	27.59
KRH76377	Soybean	cytosol	26.68	27.33
KRH07697	Soybean	nucleus	26.68	27.27
KRH39665	Soybean	cytosol	26.9	27.25
KRH07201	Soybean	cytosol	24.08	26.68
KRH57202	Soybean	nucleus	29.93	23.96
KRH03917	Soybean	nucleus	29.28	23.6
KRH42643	Soybean	nucleus	32.1	19.87
KRH58745	Soybean	nucleus	32.1	19.84
KRH31446	Soybean	nucleus	31.45	19.54
KRG97452	Soybean	nucleus	24.73	18.21

Protein Annotations

Gene3D:1.10.510.10	EntrezGene:100807993	MapMan:13.1.1.2.5	MapMan:15.3.3.4.3	MapMan:18.4.3.1.5	Gene3D:3.30.200.20
EMBL:ACUP02004743	EnsemblPlantsGene:GLYMA_08G002900	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672
GO:GO:0004674	GO:GO:0004693	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005634	GO:GO:0005654	GO:GO:0006464	GO:GO:0006468	GO:GO:0007049
GO:GO:0007346	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301	GO:GO:0016592
GO:GO:0016740	GO:GO:0019538	UniProt:I1KNX7	InterPro:IPR000719	EnsemblPlants:KRH40974	ProteinID:KRH40974
ProteinID:KRH40974.1	InterPro:Kinase-like_dom_sf	PFAM:PF00069	PIRSF:PIRSF000654	ScanProsite:PS00107	ScanProsite:PS00108
PFscan:PS50011	PANTHER:PTHR24056	PANTHER:PTHR24056:SF92	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220
SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI00023389DA	:	:	:

Description

hypothetical protein

Coordinates

chr8:-:210761..221091

Molecular Weight (calculated)

51551.2 Da

IEP (calculated)

9.532

GRAVY (calculated)

-0.401

Length

461 amino acids

Sequence
(BLAST)

001: MGDGSGNRWS RAEWVQQYDL LGKIGEGTYG LVFLARTKGT PSKSIAIKKF KQSKDGDGVS PTAIREIMLL REITHENVVK LVNVHINHAD MSLYLAFDYA
101: EHDLYEIIRH HRDKLNHSIN QYTVKSLLWQ LLNGLSYLHS NWMIHRDLKP SNILVMGEGE EHGVVKIADF GLARIYQAPL KPLSDNGVVV TIWYRAPELL
201: LGAKHYTSAV DMWAVGCIFA ELLTLKPLFQ GAEVKATSNP FQLDQLDKIF KVLGHPTLEK WPSLASLPHW QQDVQHIQGH KYDNAGLYNV VHLSPKSPAY
301: DLLSKMLEYD PRKRLTAAQA LEHEYFKIEP LPGRNALVPC QLGEKIVNYP TRPVDTTTDL EGTTNLPPSQ TVNAVSGSMP GPHGSNRSVP RPVNVVGMQR
401: MPPQAMAAYN LSSQAAMGDG MNPGGISKQR GVPQAHQPQQ LRRKEQMGMP GYPAQQKSRR I

Best Arabidopsis Sequence Match ( AT5G63610.1 )
(BLAST)

001: MGDGSSSRSN SSNSTSEKPE WLQQYNLVGK IGEGTYGLVF LARTKTPPKR PIAIKKFKQS KDGDGVSPTA IREIMLLREI SHENVVKLVN VHINFADMSL
101: YLAFDYAEYD LYEIIRHHRD KVGHSLNTYT VKSLLWQLLN GLNYLHSNWI IHRDLKPSNI LVMGDAEEHG IVKIADFGLA RIYQAPLKPL SDNGVVVTIW
201: YRAPELLLGS KHYTSAVDMW AVGCIFAELL TLKPLFQGAE AKSSQNPFQL DQLDKIFKIL GHPTMDKWPT LVNLPHWQND VQHIQAHKYD SVGLHNVVHL
301: NQKSPAYDLL SKMLEYDPLK RITASQALEH EYFRMDPLPG RNAFVASQPM EKNVNYPTRP VDTNTDFEGT TSINPPQAVA AGNVAGNMAG AHGMGSRSMP
401: RPMVAHNMQR MQQSQGMMAY NFPAQAGLNP SVPLQQQRGM AQPHQQQQLR RKDPGMGMSG YAPPNKSRRL

Arabidopsis Description

CDKE-1Cyclin-dependent kinase E-1 [Source:UniProtKB/Swiss-Prot;Acc:Q84TI6]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.