CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
mitochondrion 1
cytosol 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	nucleus
MultiLoc:	nucleus
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH25503
KRH56025

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G57150.1	KRH25503	AT1G03530.1	17712600
AT3G57150.1	KRH56025	AT1G03530.1	17712600

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH10580	Soybean	nucleus	87.76	86.42
TraesCS2D01G156900.1	Wheat	cytosol	67.07	73.4
Bra003270.1-P	Field mustard	cytosol	68.28	72.39
CDX67680	Canola	cytosol	68.28	72.39
Os04t0576300-01	Rice	nucleus, plastid	24.83	72.36
AT3G57150.1	Thale cress	cytosol	70.17	72.04
CDY33839	Canola	cytosol	64.48	71.78
CDY25279	Canola	cytosol	64.66	71.29
CDY50671	Canola	cytosol	64.48	71.1
VIT_08s0007g00190.t01	Wine grape	cytosol	71.55	70.82
PGSC0003DMT400018869	Potato	cytosol	69.66	70.51
TraesCS2D01G157000.1	Wheat	nucleus	67.41	70.32
Os11t0598700-00	Rice	mitochondrion	24.31	69.8
Bra033149.1-P	Field mustard	cytosol	65.52	69.47
GSMUA_Achr11P...	Banana	cytosol	59.83	68.44
Os03t0370500-02	Rice	nucleus	68.97	67.68
EES02707	Sorghum	cytosol	67.93	67.47
Zm00001d008327_P001	Maize	plasma membrane	67.59	67.24
EER99769	Sorghum	cytosol, nucleus, plastid	67.76	67.06
Zm00001d040293_P001	Maize	cytosol, nucleus, plastid	66.55	66.67
Solyc02g081810.2.1	Tomato	nucleus	68.45	66.5
Zm00001d022265_P001	Maize	cytosol, nucleus, plastid	65.69	65.35
Os07t0636000-01	Rice	nucleus	67.59	65.33
TraesCS2A01G151900.1	Wheat	cytosol, nucleus, plastid	67.76	64.96
TraesCS2B01G177000.1	Wheat	nucleus	67.59	64.58
TraesCS2A01G151800.1	Wheat	cytosol	67.41	64.31
GSMUA_Achr11P...	Banana	nucleus	66.21	60.28
HORVU2Hr1G027370.1	Barley	cytosol	59.66	53.89
HORVU2Hr1G027400.5	Barley	plastid	59.14	50.22
GSMUA_Achr8P15080_001	Banana	nucleus	58.62	38.99
KRH56525	Soybean	plastid	6.03	7.22

Protein Annotations

EntrezGene:100780706	MapMan:17.1.1.2.2.1.1	Gene3D:2.30.130.70	Gene3D:3.30.2350.10	EMBL:ACUP02004902	ncoils:Coil
InterPro:Dyskerin-like	EnsemblPlantsGene:GLYMA_08G176900	GO:GO:0000495	GO:GO:0001522	GO:GO:0003674	GO:GO:0003676
GO:GO:0003723	GO:GO:0003824	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005634	GO:GO:0005730	GO:GO:0006139	GO:GO:0006396	GO:GO:0008150	GO:GO:0008152
GO:GO:0009451	GO:GO:0009982	GO:GO:0009987	GO:GO:0031118	GO:GO:0031120	GO:GO:0031429
GO:GO:1990481	UniProt:I1KUB2	InterPro:IPR002478	EnsemblPlants:KRH43875	ProteinID:KRH43875	ProteinID:KRH43875.1
PFAM:PF01472	PFAM:PF01509	PFAM:PF08068	PFAM:PF16198	PFscan:PS50890	InterPro:PUA
InterPro:PUA-like_sf	InterPro:PsdUridine_synth_N	InterPro:PsdUridine_synth_cat_dom_sf	SMART:SM00359	SMART:SM01136	SUPFAM:SSF55120
SUPFAM:SSF88697	TIGRFAMs:TIGR00425	TIGRFAMs:TIGR00451	InterPro:TruB_C	UniParc:UPI000294B756	InterPro:Uncharacterised_CHP00451
SEG:seg	InterPro:tRNA_PsdUridine_synth_B_fam	:	:	:	:

Description

hypothetical protein

Coordinates

chr8:+:14181488..14183757

Molecular Weight (calculated)

65250.0 Da

IEP (calculated)

9.972

GRAVY (calculated)

-0.737

Length

580 amino acids

Sequence
(BLAST)

001: MSESDTKKKK QQQRSGGKHE GEEGLMIKPQ SFTPPIDTSQ WPILLKNYER LNVRTGHYTP IPSGYSPLKR PLSEYLKYGV LNLDKPANPS SHEVVAWIKR
101: LLRVEKTGHS GTLDPKVTGN LIVCIDRATR LVKSQQGAGK EYVCIARLHS AVPDISKVSR ALETLTGAVF QRPPLISAVK RQLRIRTIYE SKLLEYDPDR
201: HLVVFWISCE AGTYVRTMCV HLGLLLGVGS HMQELRRVRS GIMGEKDNMV SMHDVMDAQW VYDNYRDETY LRRVVMPLEI LLTSYKRLVV KDSAVNAICY
301: GAKFMIPGLL RFENDIDVGE EVVLMTTKGE AIALGIAEMT TAVMATCDHG VVAKIKRVVM DRDTYPRKWG LGPRASMKKR LISEGKLDKH GKPNEKTPQE
401: WLRNLVLPTG GDSMIAGLAA APEPEDDKLQ KKEEGDGEGK KRKKHESTDS LVAVPAKKAK VVDEVEELEK LEKVKVKKVD EETVEVEVEK KEKKKKKKKD
501: KENSEVASSD EEKTEKKKNK NKIEDGSPEL DKSEKKKKKK KDKAAAAAAE ISNGKVDDSN ADKSEKKKNK KKKNKDAKEE

Best Arabidopsis Sequence Match ( AT3G57150.1 )
(BLAST)

001: MAEVDISHSK KKKQDKTEND AADTGDYMIK PQSFTPAIDT SQWPILLKNY DRLNVRTGHY TPISAGHSPL KRPLQEYIRY GVINLDKPAN PSSHEVVAWI
101: KRILRVEKTG HSGTLDPKVT GNLIVCIDRA TRLVKSQQGA GKEYVCVARL HSAVPDVAKV ARALESLTGA VFQRPPLISA VKRQLRIRTI YESKLLEYDA
201: DRHLVVFWVS CEAGTYIRTM CVHLGLLLGV GGHMQELRRV RSGILGENNN MVTMHDVMDA QFVYDNSRDE SYLRRVIMPL EMILTSYKRL VVKDSAVNAI
301: CYGAKLMIPG LLRFENDIDV GTEVVLMTTK GEAIAVGIAE MTTSVMATCD HGVVAKIKRV VMDRDTYPRK WGLGPRASMK KKLIADGKLD KHGKPNEKTP
401: VEWSRNVVLP TGGDAIIAGA AAAPEEIKAD AENGEAGEAR KRKHDDSSDS PAPVTTKKSK TKEVEGEEAE EKVKSSKKKK KKDKEEEKEE EAGSEKKEKK
501: KKKDKKEEVI EEVASPKSEK KKKKKSKDTE AAVDAEDESA AEKSEKKKKK KDKKKKNKDS EDDEE

Arabidopsis Description

CBF5Uncharacterized protein At3g57150 (Fragment) [Source:UniProtKB/TrEMBL;Acc:C0SVF3]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.