CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 1
mitochondrion 2
cytosol 3
plastid 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 23041469
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH41811
KRH60516
KRH68073

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G25780.1	KRH41811	AT3G56150.1	11029466
AT5G25780.1	KRH60516	AT3G56150.1	11029466
AT5G25780.1	KRH68073	AT3G56150.1	11029466

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH64883	Soybean	endoplasmic reticulum	98.19	98.19
KRH61614	Soybean	endoplasmic reticulum	76.32	92.1
PGSC0003DMT400004200	Potato	cytosol	81.2	81.31
Solyc01g088370.2.1	Tomato	nucleus, plastid	80.78	80.89
GSMUA_Achr11P...	Banana	cytosol	75.63	78.24
EER91898	Sorghum	cytosol	77.16	76.94
Zm00001d030011_P001	Maize	plasma membrane	77.02	76.81
Os10t0569200-01	Rice	cytosol, golgi, nucleus, plasma membrane, plastid	76.6	76.5
TraesCS5A01G442500.1	Wheat	cytosol, golgi	75.21	75.52
Bra007832.1-P	Field mustard	cytosol	74.93	75.35
HORVU5Hr1G106440.1	Barley	cytosol	75.63	75.31
TraesCS5D01G449900.2	Wheat	cytosol, nucleus, unclear	75.49	75.17
AT5G25780.1	Thale cress	cytosol	74.65	75.07
TraesCS5B01G446600.1	Wheat	cytosol	75.35	75.03
Bra032059.1-P	Field mustard	cytosol	74.51	74.93
HORVU4Hr1G014100.3	Barley	cytosol	74.65	74.34
AT5G27640.2	Thale cress	cytosol	75.91	73.85
TraesCS4D01G081300.1	Wheat	cytosol, nucleus	73.96	73.65
TraesCS4A01G232600.1	Wheat	cytosol	73.68	73.37
TraesCS4B01G083200.1	Wheat	cytosol	72.01	73.33
TraesCS6B01G307400.1	Wheat	cytosol	69.64	72.67
TraesCS6D01G406100.1	Wheat	cytosol	35.65	55.77
AT1G49015.1	Thale cress	cytosol	15.6	49.34
Bra009732.1-P	Field mustard	cytosol	25.63	36.29
HORVU5Hr1G008250.6	Barley	cytosol, mitochondrion, plastid	44.57	36.04
KRH75983	Soybean	cytosol	5.29	29.23
KRH71216	Soybean	nucleus	13.51	18.73
KRH50214	Soybean	nucleus	13.09	18.18
KRH66844	Soybean	cytosol	1.53	14.1

Protein Annotations

EntrezGene:100817394	MapMan:17.4.1.3.2	Gene3D:2.130.10.10	Gene3D:3.30.70.330	EMBL:ACUP02004270	InterPro:EIF3B
EnsemblPlantsGene:GLYMA_07G107700	GO:GO:0001731	GO:GO:0001732	GO:GO:0002183	GO:GO:0003674	GO:GO:0003676
GO:GO:0003723	GO:GO:0003743	GO:GO:0005488	GO:GO:0005515	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005852	GO:GO:0006412	GO:GO:0006413	GO:GO:0006446
GO:GO:0008135	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016043
GO:GO:0016282	GO:GO:0019538	GO:GO:0031369	GO:GO:0033290	UniProt:I1KJQ5	InterPro:IPR000504
InterPro:IPR012677	InterPro:IPR015943	EnsemblPlants:KRH48727	ProteinID:KRH48727	ProteinID:KRH48727.1	HAMAP:MF_03001
InterPro:Nucleotide-bd_a/b_plait_sf	PFAM:PF00076	PFAM:PF08662	PIRSF:PIRSF036424	PFscan:PS50102	PANTHER:PTHR14068
InterPro:RBD_domain_sf	InterPro:RRM_dom	SMART:SM00360	SUPFAM:SSF54928	SUPFAM:SSF82171	InterPro:TIF_beta_prop-like
UniParc:UPI0002338AAE	InterPro:WD40/YVTN_repeat-like_dom_sf	InterPro:eIF3B_RRM	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr7:-:10574234..10578530

Molecular Weight (calculated)

82650.7 Da

IEP (calculated)

4.896

GRAVY (calculated)

-0.530

Length

718 amino acids

Sequence
(BLAST)

001: MADVMVMKEI EDTALRLGVD LSTLDLDAIR LPPGEDCGIV SDDEVVYQEE NLEFESGFGN IIVVDNLPVV PREKFEKLEG VVRKIYSQIG VIKEDGLWMP
101: VDPETEKTLG YCFIEYNTPQ EAELAKEKTH GYKLDRAHIF AVSMFDDFDR FMKVPNEWAP PETKPYAPGE NLQHWLTDAK ARDQFVIRAG SDTEVLWNDA
201: RHLKPDPVYK RAFWTESFVQ WSPLGTYLAT VHRQGAAVWG GAASFNRLMR YAHPQVKLID FSPGEKYLVT YSSHEPSNPR DANRVVINIF DVRTGKVMRD
301: FKGSADDFAV GGAGGVTGVS WPVFKWSGGR DDKYFARMGK NILSVYEAET FSLVDKKSLK VENIMDFCWS PTDPIIALFV PEMGGGNQPA RVSLIQIPSK
401: EELRQKNLFS VSDCKIYWQS NGDYLAVNVE RYTKTKKSTY TGFELFRIKE RDIPIEVLEL ENKNDKIIAF AWEPKGHRFA VIHGDNPKPD VSIYSMRTGQ
501: NSRVSKLITL KGKQANALFW SPAGRYIVLA GLKGFNGQLE FYNVDELETM ATAEHFMATD IEWDPTGRYV ATAVTSVHEM ENGFNIWSFN GKHLYRILKD
601: HFFQFLWRPR PPSFLTPEKE EEIAKNLKKY SKKYEAEDQD VSLLLSEQER EKRRMLKEEW DKWVNEWKQI HEEESLQRQK LRDGEASDEE EEYEAKDIEV
701: EEVIDVTKEV LHLEYGQE

Best Arabidopsis Sequence Match ( AT5G27640.1 )
(BLAST)

001: MEVVDIDARA AKLGIDWSQV NFDSIQLPPG EDFGIESDDE AVYQDDQSEF DTGFGNIIVV DHLPVVPKEK FEKLEGVVKK IYNQLGVIKE NGLWMPVDPD
101: TKMTLGYCFI EFNTPQEAQN AKEKSHGYKL DKSHIFAVNM FDDFDRLMNV KEEWEPPQAR PYVPGENLQK WLTDEKARDQ LVIRSGPDTE VFWNDTRQKA
201: PEPVHKRPYW TESYVQWSPL GTYLVTLHKQ GAAVWGGADT FTRLMRYQHS MVKLVDFSPG EKYLVTYHSQ EPSNPRDASK VEIKVFDVRT GRMMRDFKGS
301: ADEFSIGGPG GVAGASWPVF RWAGGKDDKY FAKLSKNTIS VYETETFSLI DKKSMKVDNV VDICWSPTDS ILSLFVPEQG GGNQPAKVAL VQIPSKVELR
401: QKNLFSVSDC KMYWQSSGEY LAVKVDRYTK TKKSTYSGFE LFRIKERDIP IEVLELDNKN DKIIAFAWEP KGHRFAVIHG DQPRPDVSFY SMKTAQNTGR
501: VSKLATLKAK QANALFWSPT GKYIILAGLK GFNGQLEFFN VDELETMATA EHFMATDIEW DPTGRYVATA VTSVHEMENG FTIWSFNGIM LYRILKDHFF
601: QLAWRPRPPS FLTAEKEEEI AKTLKKYSKK YEAEDQDVSL LLSEQDREKR KALKEEWEKW VMQWKSLHEE EKLVRQNLRD GEVSDVEEDE YEAKEVEFED
701: LIDVTEEIVQ ES

Arabidopsis Description

TIF3B1Eukaryotic translation initiation factor 3 subunit B [Source:UniProtKB/TrEMBL;Acc:F4K4D5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.