CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 2
mitochondrion 2
peroxisome 2
cytosol 1

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 27224218

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH41811
KRH60516
KRH68073

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G25780.1	KRH41811	AT3G56150.1	11029466
AT5G25780.1	KRH60516	AT3G56150.1	11029466
AT5G25780.1	KRH68073	AT3G56150.1	11029466

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH48727	Soybean	endoplasmic reticulum	92.1	76.32
KRH64883	Soybean	endoplasmic reticulum	91.43	75.77
PGSC0003DMT400004200	Potato	cytosol	77.65	64.44
Solyc01g088370.2.1	Tomato	nucleus, plastid	76.97	63.88
GSMUA_Achr11P...	Banana	cytosol	71.43	61.24
Os10t0569200-01	Rice	cytosol, golgi, nucleus, plasma membrane, plastid	73.11	60.5
EER91898	Sorghum	cytosol	73.11	60.42
Zm00001d030011_P001	Maize	plasma membrane	72.61	60.0
TraesCS5A01G442500.1	Wheat	cytosol, golgi	71.6	59.58
AT5G25780.1	Thale cress	cytosol	71.09	59.24
HORVU5Hr1G106440.1	Barley	cytosol	71.76	59.22
TraesCS5D01G449900.2	Wheat	cytosol, nucleus, unclear	71.6	59.08
TraesCS5B01G446600.1	Wheat	cytosol	71.6	59.08
Bra007832.1-P	Field mustard	cytosol	70.76	58.96
Bra032059.1-P	Field mustard	cytosol	70.59	58.82
HORVU4Hr1G014100.3	Barley	cytosol	70.59	58.25
TraesCS4B01G083200.1	Wheat	cytosol	68.74	58.01
AT5G27640.2	Thale cress	cytosol	71.93	57.99
TraesCS4A01G232600.1	Wheat	cytosol	69.75	57.56
TraesCS4D01G081300.1	Wheat	cytosol, nucleus	69.58	57.42
TraesCS6B01G307400.1	Wheat	cytosol	65.04	56.25
TraesCS6D01G406100.1	Wheat	cytosol	34.79	45.1
AT1G49015.1	Thale cress	cytosol	14.79	38.77
KRH75983	Soybean	cytosol	6.39	29.23
Bra009732.1-P	Field mustard	cytosol	24.71	28.99
HORVU5Hr1G008250.6	Barley	cytosol, mitochondrion, plastid	42.02	28.15
KRH71216	Soybean	nucleus	13.28	15.25
KRH50214	Soybean	nucleus	12.94	14.89
KRH66844	Soybean	cytosol	1.85	14.1

Protein Annotations

Gene3D:2.130.10.10	Gene3D:3.30.70.330	MapMan:35.1	EMBL:ACUP02002174	InterPro:EIF3B	EnsemblPlantsGene:GLYMA_04G057600
GO:GO:0001731	GO:GO:0001732	GO:GO:0002183	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723
GO:GO:0003743	GO:GO:0005488	GO:GO:0005515	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005852	GO:GO:0006412	GO:GO:0006413	GO:GO:0006446	GO:GO:0008135
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016043	GO:GO:0016282
GO:GO:0019538	GO:GO:0031369	GO:GO:0033290	InterPro:IPR000504	InterPro:IPR012677	InterPro:IPR015943
UniProt:K7KIC0	EnsemblPlants:KRH61614	ProteinID:KRH61614	ProteinID:KRH61614.1	HAMAP:MF_03001	InterPro:Nucleotide-bd_a/b_plait_sf
PFAM:PF00076	PFAM:PF08662	PANTHER:PTHR14068	InterPro:RBD_domain_sf	InterPro:RRM_dom	SUPFAM:SSF54928
SUPFAM:SSF82171	InterPro:TIF_beta_prop-like	UniParc:UPI000296CC8F	InterPro:WD40/YVTN_repeat-like_dom_sf	InterPro:eIF3B_RRM	SEG:seg

Description

hypothetical protein

Coordinates

chr4:-:4679730..4685267

Molecular Weight (calculated)

68820.8 Da

IEP (calculated)

5.804

GRAVY (calculated)

-0.559

Length

595 amino acids

Sequence
(BLAST)

001: MQQHEKRRIR GCDLVPREKF EKLEGVVRKI YSQIGVIKED GLWMPIYPET EKTLGYCFIE YNTPQEAELA KEKTHGYKLD RAHIFAVSMF DDFDRFMKVP
101: NEWAPPETKP YTPGENLQDW LTDAKARDQF VIRAGSDTEV LWNDARHLKP DPVYKRAFGQ SFVQWSPLGT YLATVHRQGA AVWGGAASFN KLMRYSHPQV
201: KLIDFSPGEK YLVTYISHEP SNPRDANRVV INIFDVRTGK VMRDLGSADD FAVGGAGGVT GVSWSVFKYY LVKVENIMDF CWSSTDPIIA LVVPDMGGGN
301: QPARVSLIQI PSKEELRQKN LFSVGDCKIT YTGFELFRIK ERDIPIEVLE LENKNDKIIA FAWEPKGHRF AVIHGDNPKP NVIYSMRTGQ NSQVSKLTTL
401: KGKQANALFW SPAGRYIVLA GLKGFNGQLE FYNVDELKTM ATAEHFMATD IEWDPTGRMV SVWSFNGKHL YRILKDHLFQ FLWRPRPPSF LTPEKEEEIA
501: KNLKKYSIYE AEDQDVSLPL SEQEREKCRM LKEDWDKWVN EWKQIHEESL QRQKLRDGEA SDEEEEYEAK DMEVEEVMDV TEEVLHLEYG HWSRV

Best Arabidopsis Sequence Match ( AT5G27640.1 )
(BLAST)

001: MEVVDIDARA AKLGIDWSQV NFDSIQLPPG EDFGIESDDE AVYQDDQSEF DTGFGNIIVV DHLPVVPKEK FEKLEGVVKK IYNQLGVIKE NGLWMPVDPD
101: TKMTLGYCFI EFNTPQEAQN AKEKSHGYKL DKSHIFAVNM FDDFDRLMNV KEEWEPPQAR PYVPGENLQK WLTDEKARDQ LVIRSGPDTE VFWNDTRQKA
201: PEPVHKRPYW TESYVQWSPL GTYLVTLHKQ GAAVWGGADT FTRLMRYQHS MVKLVDFSPG EKYLVTYHSQ EPSNPRDASK VEIKVFDVRT GRMMRDFKGS
301: ADEFSIGGPG GVAGASWPVF RWAGGKDDKY FAKLSKNTIS VYETETFSLI DKKSMKVDNV VDICWSPTDS ILSLFVPEQG GGNQPAKVAL VQIPSKVELR
401: QKNLFSVSDC KMYWQSSGEY LAVKVDRYTK TKKSTYSGFE LFRIKERDIP IEVLELDNKN DKIIAFAWEP KGHRFAVIHG DQPRPDVSFY SMKTAQNTGR
501: VSKLATLKAK QANALFWSPT GKYIILAGLK GFNGQLEFFN VDELETMATA EHFMATDIEW DPTGRYVATA VTSVHEMENG FTIWSFNGIM LYRILKDHFF
601: QLAWRPRPPS FLTAEKEEEI AKTLKKYSKK YEAEDQDVSL LLSEQDREKR KALKEEWEKW VMQWKSLHEE EKLVRQNLRD GEVSDVEEDE YEAKEVEFED
701: LIDVTEEIVQ ES

Arabidopsis Description

TIF3B1Eukaryotic translation initiation factor 3 subunit B [Source:UniProtKB/TrEMBL;Acc:F4K4D5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.