Factsheet for Os02t0167300-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 4
extracellular 1
mitochondrion 2
nucleus 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 19502382
extracellular: 26072143
plasma membrane: 28056797

msms PMID: 26072143 doi

B Du, Z Wei, Z Wang, X Wang, X Peng, B Du, R Chen, L Zhu, G He

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China., State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: gche@whu.edu.cn.

msms PMID: 19502382 doi

M Fujiwara, S Hamada, M Hiratsuka, Y Fukao, T Kawasaki, K Shimamoto

Laboratory of Plant Protein Analysis, Plant Education Unit, Nara Institute of Science and Technology, Takayama, Ikoma, Japan.

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0816500-01
Os05t0255600-01
Os11t0604700-00

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G20890.1	Os02t0816500-01	AT2G30410.1	20638386
AT5G44340.1	Os05t0255600-01	AT3G50960.1	18390592
AT5G44340.1	Os11t0604700-00	AT3G50960.1	18390592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d036626_P001	Maize	cytosol	50.11	98.68
Os06t0671900-01	Rice	plasma membrane	97.32	97.53
KRH58404	Soybean	nucleus	96.42	97.51
GSMUA_Achr11P...	Banana	cytosol	76.29	97.43
Zm00001d046996_P001	Maize	cytosol	50.34	97.4
KRH36829	Soybean	nucleus	97.09	97.31
KRH11812	Soybean	nucleus	97.09	97.31
VIT_10s0003g05320.t01	Wine grape	cytosol	96.64	97.08
KRH42279	Soybean	cytosol	96.2	97.07
GSMUA_Achr5P26680_001	Banana	cytosol	84.12	96.91
Os03t0780600-01	Rice	plastid	96.2	96.85
GSMUA_Achr2P01410_001	Banana	cytosol	84.34	96.67
GSMUA_Achr3P23490_001	Banana	cytosol	80.31	96.25
CDY31638	Canola	cytosol	95.53	96.17
GSMUA_Achr8P07060_001	Banana	cytosol	83.67	96.14
CDX84283	Canola	cytosol	95.3	95.52
GSMUA_Achr5P00290_001	Banana	cytosol	83.45	94.91
Os01t0805900-01	Rice	plasma membrane	94.85	94.85
Os03t0105600-01	Rice	plasma membrane	94.85	94.85
Os05t0413200-01	Rice	plasma membrane	93.74	94.37
Os01t0282800-02	Rice	plasma membrane	94.18	94.18
CDX94014	Canola	cytosol	93.74	93.95
AT5G44340.1	Thale cress	cytosol	93.29	93.92
CDX98679	Canola	cytosol	93.51	93.72
Bra020912.1-P	Field mustard	cytosol	93.51	93.72
AT4G20890.1	Thale cress	cytosol	93.06	93.69
CDX76444	Canola	cytosol	93.29	93.5
CDY31428	Canola	cytosol	93.29	93.5
CDY60599	Canola	cytosol	93.06	93.48
CDX96965	Canola	cytosol	92.84	93.26
Bra038795.1-P	Field mustard	cytosol	92.84	93.26
Bra027544.1-P	Field mustard	cytosol	92.84	93.26
Bra039506.1-P	Field mustard	cytosol	92.84	93.05
CDX79013	Canola	cytosol	92.84	93.05
CDX82788	Canola	cytosol	92.84	93.05
CDY14924	Canola	cytosol	92.84	93.05
CDY13108	Canola	cytosol	92.62	93.03
CDY69221	Canola	cytosol	92.62	93.03
Bra033737.1-P	Field mustard	cytosol	92.39	92.81
CDY49002	Canola	cytosol	92.39	92.81
Os03t0661300-01	Rice	plasma membrane	91.72	91.93
Bra013481.1-P	Field mustard	cytosol	91.72	91.93
Bra005395.1-P	Field mustard	cytosol	80.54	89.33
GSMUA_Achr6P19990_001	Banana	cytosol	79.19	85.51
Os03t0726100-01	Rice	nucleus	42.73	42.35
Os07t0574800-02	Rice	plasma membrane	42.06	41.78
Os11t0247300-01	Rice	plasma membrane	42.06	41.69
Os03t0219300-01	Rice	cytosol, plasma membrane, plastid	41.16	40.98
Os05t0156600-01	Rice	cytosol	34.68	33.05
Os03t0780800-01	Rice	cytosol	2.24	4.22

Protein Annotations

Gene3D:1.10.287.600	MapMan:20.1.1.2	Gene3D:3.30.1330.20	Gene3D:3.40.50.1440	EntrezGene:4328420	EMBL:AK121574
ProteinID:BAD26239.1	ProteinID:BAF07920.1	ProteinID:BAS77161.1	InterPro:Beta-tubulin_BS	InterPro:Beta_tubulin	ncoils:Coil
EMBL:D30717	ProteinID:EAZ21874.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0003924
GO:GO:0005198	GO:GO:0005200	GO:GO:0005488	GO:GO:0005525	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005856	GO:GO:0005874	GO:GO:0007010	GO:GO:0007017
GO:GO:0008150	GO:GO:0009987	GO:GO:0016043	GO:GO:0016787	InterPro:IPR023123	InterPro:IPR036525
InterPro:IPR037103	EnsemblPlantsGene:Os02g0167300	EnsemblPlants:Os02t0167300-01	UniProt:P46265	PFAM:PF00091	PFAM:PF03953
PRINTS:PR01161	PRINTS:PR01163	ScanProsite:PS00227	ScanProsite:PS00228	PANTHER:PTHR11588	PANTHER:PTHR11588:SF245
SMART:SM00864	SMART:SM00865	SUPFAM:SSF52490	SUPFAM:SSF55307	InterPro:Tub_FtsZ_C	InterPro:Tubulin
InterPro:Tubulin/FtsZ_2-layer-sand-dom	InterPro:Tubulin/FtsZ_C_sf	InterPro:Tubulin/FtsZ_GTPase_sf	InterPro:Tubulin_C	InterPro:Tubulin_CS	InterPro:Tubulin_FtsZ_GTPase
UniParc:UPI00001369F1	EMBL:X79367	RefSeq:XP_015625553.1	SEG:seg	:	:

Description

BETA-TUBULIN 5Tubulin beta-3 chain (Beta-3 tubulin). (Os02t0167300-01);Similar to Tubulin beta-5 chain. (Os02t0167300-02)

Coordinates

chr2:+:3619840..3622862

Molecular Weight (calculated)

50108.1 Da

IEP (calculated)

4.457

GRAVY (calculated)

-0.358

Length

447 amino acids

Sequence
(BLAST)

001: MREILHIQGG QCGNQIGAKF WEVICDEHGI DHTGKYSGDS DLQLERINVY YNEASGGRFV PRAVLMDLEP GTMDSVRSGP FGQIFRPDNF VFGQSGAGNN
101: WAKGHYTEGA ELIDSVLDVV RKEAENCDCL QGFQVCHSLG GGTGSGMGTL LISKIREEYP DRMMLTFSVF PSPKVSDTVV EPYNATLSVH QLVENADECM
201: VLDNEALYDI CFRTLKLATP TFGDLNHLIS ATMSGVTCCL RFPGQLNSDL RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YRALTVPELT QQMWDAKNMM
301: CAADPRHGRY LTASAMFRGK MSTKEVDEQM LNVQNKNSSY FVEWIPNNVK SSVCDIPPNG LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG
401: EGMDEMEFTE AESNMNDLVA EYQQYQDATA DDEEEDYGDE EEDEVAA

Best Arabidopsis Sequence Match ( AT5G23860.2 )
(BLAST)

001: MREILHIQGG QCGNQIGAKF WEVVCAEHGI DSTGRYQGEN DLQLERVNVY YNEASCGRFV PRAVLMDLEP GTMDSVRSGP YGQIFRPDNF VFGQSGAGNN
101: WAKGHYTEGA ELIDSVLDVV RKEAENCDCL QGFQVCHSLG GGTGSGMGTL LISKIREEYP DRMMLTFSVF PSPKVSDTVV EPYNATLSVH QLVENADECM
201: VLDNEALYDI CFRTLKLTTP SFGDLNHLIS ATMSGVTCCL RFPGQLNSDL RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YRALTVPELT QQMWDAKNMM
301: CAADPRHGRY LTASAMFRGK MSTKEVDEQM INVQNKNSSY FVEWIPNNVK STVCDIPPTG LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG
401: EGMDEMEFTE AESNMNDLVS EYQQYQDATA DEEEGYEYEE DEVEVQEEQ

Arabidopsis Description

TUBB8Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:A0A178UKM5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.