CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 4
extracellular 1
mitochondrion 2
nucleus 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH17436
KRH22160
KRH27177
KRH69862

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G20890.1	KRH22160	AT2G30410.1	20638386
AT4G20890.1	KRH27177	AT2G30410.1	20638386
AT5G44340.1	KRH17436	AT3G50960.1	18390592
AT5G44340.1	KRH69862	AT3G50960.1	18390592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH11812	Soybean	nucleus	99.55	99.55
KRH58404	Soybean	nucleus	97.53	98.42
GSMUA_Achr11P...	Banana	cytosol	77.13	98.29
Os06t0671900-01	Rice	plasma membrane	98.21	98.21
EES04582	Sorghum	cytosol	97.98	97.98
VIT_10s0003g05320.t01	Wine grape	cytosol	97.76	97.98
KRH42279	Soybean	cytosol	97.31	97.97
GSMUA_Achr5P26680_001	Banana	cytosol	84.98	97.68
Zm00001d036626_P001	Maize	cytosol	49.55	97.36
EER90205	Sorghum	cytosol	97.31	97.31
GSMUA_Achr2P01410_001	Banana	cytosol	84.98	97.18
Os02t0167300-01	Rice	plasma membrane	97.31	97.09
CDY31638	Canola	cytosol	96.64	97.07
GSMUA_Achr3P23490_001	Banana	cytosol	81.17	97.05
GSMUA_Achr8P07060_001	Banana	cytosol	84.3	96.66
CDX84283	Canola	cytosol	96.64	96.64
TraesCS6D01G130500.2	Wheat	golgi	96.41	96.63
Os03t0780600-01	Rice	plastid	96.19	96.62
Zm00001d015348_P002	Maize	plastid	96.19	96.62
HORVU6Hr1G027620.1	Barley	plasma membrane	96.41	96.41
EER93354	Sorghum	cytosol	96.19	96.4
TraesCS6B01G169300.1	Wheat	cytosol	95.96	96.4
Zm00001d046996_P001	Maize	cytosol	49.78	96.1
GSMUA_Achr5P00290_001	Banana	cytosol	84.53	95.93
TraesCS5B01G418700.1	Wheat	golgi	95.52	95.73
TraesCS5D01G424100.1	Wheat	cytosol	95.52	95.73
TraesCS5A01G416400.1	Wheat	cytosol	95.07	95.5
KRH65977	Soybean	nucleus	95.96	95.32
TraesCS7A01G466600.1	Wheat	golgi	95.29	95.29
TraesCS7D01G454200.1	Wheat	cytosol	95.29	95.29
Zm00001d013139_P001	Maize	cytosol	62.56	95.22
KRH75786	Soybean	nucleus	95.74	95.1
HORVU7Hr1G107100.2	Barley	cytosol, nucleus	95.07	95.07
AT5G44340.1	Thale cress	cytosol	94.39	94.82
CDX94014	Canola	cytosol	94.39	94.39
CDX96965	Canola	cytosol	94.17	94.38
Bra027544.1-P	Field mustard	cytosol	94.17	94.38
KRG91525	Soybean	nucleus	94.62	94.2
KRH35301	Soybean	nucleus	94.62	94.2
CDY14924	Canola	cytosol	94.17	94.17
CDX98679	Canola	cytosol	94.17	94.17
Bra020912.1-P	Field mustard	cytosol	94.17	94.17
Bra039506.1-P	Field mustard	cytosol	94.17	94.17
CDY13108	Canola	cytosol	93.95	94.16
CDY60599	Canola	cytosol	93.95	94.16
AT4G20890.1	Thale cress	cytosol	93.72	94.14
CDY31428	Canola	cytosol	93.95	93.95
CDX76444	Canola	cytosol	93.95	93.95
CDY69221	Canola	cytosol	93.72	93.93
Zm00001d034022_P001	Maize	cytosol	55.16	93.89
KRH05958	Soybean	nucleus	94.39	93.76
KRH51701	Soybean	nucleus	94.17	93.75
Bra033737.1-P	Field mustard	cytosol	93.5	93.71
CDY49002	Canola	cytosol	93.5	93.71
Bra038795.1-P	Field mustard	cytosol	93.5	93.71
CDX82788	Canola	cytosol	93.5	93.5
CDX79013	Canola	cytosol	93.5	93.5
KRG95065	Soybean	plasma membrane	94.17	93.33
KRH66728	Soybean	nucleus	93.95	93.32
KRH61035	Soybean	nucleus	93.5	92.87
KRH41834	Soybean	cytosol	92.38	92.58
GSMUA_Achr6P04600_001	Banana	cytosol	87.67	92.44
Bra013481.1-P	Field mustard	cytosol	92.38	92.38
KRH17435	Soybean	nucleus	94.62	92.34
KRH60536	Soybean	endoplasmic reticulum	91.93	92.13
KRH41169	Soybean	cytosol	91.03	91.24
KRH59870	Soybean	endoplasmic reticulum	90.58	90.58
Bra005395.1-P	Field mustard	cytosol	80.72	89.33
GSMUA_Achr6P19990_001	Banana	cytosol	79.6	85.75
HORVU5Hr1G100900.2	Barley	cytosol	95.52	84.19
KRH04356	Soybean	cytosol	26.68	47.41
KRH06693	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	42.38	42.66
KRH58942	Soybean	nucleus	42.83	42.54
KRG91143	Soybean	nucleus	42.83	42.54
KRH42841	Soybean	cytosol, nucleus	42.83	42.44
KRG94842	Soybean	cytosol, endoplasmic reticulum, mitochondrion	42.83	42.35
KRH35640	Soybean	cytosol, nucleus	42.6	42.32
KRH77187	Soybean	endoplasmic reticulum	42.6	42.13
KRH62139	Soybean	nucleus	42.6	42.04
KRH52851	Soybean	cytosol, nucleus	42.6	42.04
KRH28301	Soybean	cytosol, endoplasmic reticulum	42.38	41.91
KRH58181	Soybean	nucleus	42.15	41.78
KRH08505	Soybean	cytosol, endoplasmic reticulum, nucleus	41.48	41.67
KRH04357	Soybean	cytosol	13.45	35.29
KRG97131	Soybean	cytosol	34.98	32.91
KRH68880	Soybean	endoplasmic reticulum	34.98	32.91

Protein Annotations

Gene3D:1.10.287.600	EntrezGene:100818878	MapMan:20.1.1.2	Gene3D:3.30.1330.20	Gene3D:3.40.50.1440	EMBL:ACUP02005397
InterPro:Beta-tubulin_BS	InterPro:Beta_tubulin	ncoils:Coil	EnsemblPlantsGene:GLYMA_09G026100	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0003924	GO:GO:0005198	GO:GO:0005200	GO:GO:0005488	GO:GO:0005525
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005856	GO:GO:0005874
GO:GO:0007010	GO:GO:0007017	GO:GO:0008150	GO:GO:0009987	GO:GO:0016043	GO:GO:0016787
UniProt:I1L0G9	InterPro:IPR023123	InterPro:IPR036525	InterPro:IPR037103	EnsemblPlants:KRH36829	ProteinID:KRH36829
ProteinID:KRH36829.1	PFAM:PF00091	PFAM:PF03953	PRINTS:PR01161	PRINTS:PR01163	ScanProsite:PS00227
ScanProsite:PS00228	PANTHER:PTHR11588	PANTHER:PTHR11588:SF245	SMART:SM00864	SMART:SM00865	SUPFAM:SSF52490
SUPFAM:SSF55307	InterPro:Tub_FtsZ_C	InterPro:Tubulin	InterPro:Tubulin/FtsZ_2-layer-sand-dom	InterPro:Tubulin/FtsZ_C_sf	InterPro:Tubulin/FtsZ_GTPase_sf
InterPro:Tubulin_C	InterPro:Tubulin_CS	InterPro:Tubulin_FtsZ_GTPase	UniParc:UPI000233C1AC	SEG:seg	:

Description

hypothetical protein

Coordinates

chr9:-:2127403..2130528

Molecular Weight (calculated)

50167.3 Da

IEP (calculated)

4.505

GRAVY (calculated)

-0.375

Length

446 amino acids

Sequence
(BLAST)

001: MREILHIQGG QCGNQIGAKF WEVICDEHGI DHTGKYSGDS ELQLERINVY YNEASGGRYV PRAVLMDLEP GTMDSVRSGP YGQIFRPDNF VFGQSGAGNN
101: WAKGHYTEGA ELIDSVLDVV RKEAENCDCL QGFQVCHSLG GGTGSGMGTL LISKIREEYP DRMMLTFSVF PSPKVSDTVV EPYNATLSVH QLVENADECM
201: VLDNEALYDI CFRTLKLATP TFGDLNHLIS ATMSGVTCCL RFPGQLNSDL RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YRALTVPELT QQMWDAKNMM
301: CAADPRHGRY LTASAMFRGK MSTKEVDEQM INVQNKNSSY FVEWIPNNVK SSVCDIPPKG LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG
401: EGMDEMEFTE AESNMNDLVA EYQQYQDATA DEDEYEEEEE EEEIAA

Best Arabidopsis Sequence Match ( AT2G29550.1 )
(BLAST)

001: MREILHIQGG QCGNQIGSKF WEVVNLEHGI DQTGRYVGDS ELQLERVNVY YNEASCGRYV PRAVLMDLEP GTMDSVRSGP YGQIFRPDNF VFGQSGAGNN
101: WAKGHYTEGA ELIDSVLDVV RKEAENCDCL QGFQVCHSLG GGTGSGMGTL LISKIREEYP DRMMMTFSVF PSPKVSDTVV EPYNATLSVH QLVENADECM
201: VLDNEALYDI CFRTLKLSTP SFGDLNHLIS ATMSGVTCCL RFPGQLNSDL RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YRNLTVPELT QQMWDAKNMM
301: CAADPRHGRY LTASAMFRGK MSTKEVDEQM LNVQNKNSSY FVEWIPNNVK STVCDIPPTG LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG
401: EGMDEMEFTE AESNMNDLVS EYQQYQDATA DEEGEYEEEE AEYEQEETY

Arabidopsis Description

TUBB7Tubulin beta-7 chain [Source:UniProtKB/Swiss-Prot;Acc:P29515]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.