CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 4
vacuole 1
mitochondrion 2
nucleus 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH17436
KRH69862

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G20010.1	KRH17436	AT3G50960.1	18390592
AT1G20010.1	KRH69862	AT3G50960.1	18390592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH05958	Soybean	nucleus	96.94	98.66
KRH61035	Soybean	nucleus	94.31	95.99
KRH51701	Soybean	nucleus	94.09	95.98
KRH58404	Soybean	nucleus	92.56	95.7
KRH42279	Soybean	cytosol	91.9	94.81
KRH36829	Soybean	nucleus	92.34	94.62
KRH11812	Soybean	nucleus	92.34	94.62
Bra015815.1-P	Field mustard	cytosol	91.9	93.96
CDX73198	Canola	cytosol	91.9	93.96
CDX87664	Canola	cytosol	91.9	93.96
VIT_18s0001g14360.t01	Wine grape	cytosol	91.9	93.75
AT1G75780.1	Thale cress	cytosol	91.68	93.74
PGSC0003DMT400025739	Potato	cytosol	92.12	93.35
Solyc04g081490.2.1	Tomato	nucleus	91.47	92.68
KRG95065	Soybean	plasma membrane	91.25	92.67
KRH66728	Soybean	nucleus	91.03	92.65
Solyc12g089310.1.1	Tomato	nucleus	91.25	92.46
AT1G20010.1	Thale cress	cytosol	90.81	92.43
KRH65977	Soybean	nucleus	90.81	92.43
PGSC0003DMT400075425	Potato	cytosol	91.03	92.24
KRH75786	Soybean	nucleus	90.59	92.2
KRG91525	Soybean	nucleus	89.93	91.74
KRH35301	Soybean	nucleus	89.93	91.74
KRH41169	Soybean	cytosol	89.06	91.46
KRH59870	Soybean	endoplasmic reticulum	88.4	90.58
KRH41834	Soybean	cytosol	87.97	90.34
KRH60536	Soybean	endoplasmic reticulum	87.97	90.34
KRH04356	Soybean	cytosol	25.6	46.61
KRH35640	Soybean	cytosol, nucleus	41.14	41.87
KRH58942	Soybean	nucleus	41.14	41.87
KRH42841	Soybean	cytosol, nucleus	41.14	41.78
KRH06693	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	40.48	41.76
KRG91143	Soybean	nucleus	40.92	41.65
KRH62139	Soybean	nucleus	40.92	41.37
KRH52851	Soybean	cytosol, nucleus	40.92	41.37
KRH28301	Soybean	cytosol, endoplasmic reticulum	40.7	41.24
KRG94842	Soybean	cytosol, endoplasmic reticulum, mitochondrion	40.7	41.24
KRH77187	Soybean	endoplasmic reticulum	40.48	41.02
KRH08505	Soybean	cytosol, endoplasmic reticulum, nucleus	39.82	40.99
KRH58181	Soybean	nucleus	40.26	40.89
KRH04357	Soybean	cytosol	12.69	34.12
KRG97131	Soybean	cytosol	35.01	33.76
KRH68880	Soybean	endoplasmic reticulum	35.01	33.76

Protein Annotations

Gene3D:1.10.287.600	MapMan:20.1.1.2	Gene3D:3.30.1330.20	Gene3D:3.40.50.1440	EMBL:ACUP02009091	InterPro:Beta-tubulin_BS
InterPro:Beta_tubulin	ncoils:Coil	EnsemblPlantsGene:GLYMA_14G219300	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0003924	GO:GO:0005198	GO:GO:0005200	GO:GO:0005488	GO:GO:0005525	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005856	GO:GO:0005874	GO:GO:0007010
GO:GO:0007017	GO:GO:0008150	GO:GO:0009987	GO:GO:0016043	GO:GO:0016787	UniProt:I1MC39
InterPro:IPR023123	InterPro:IPR036525	InterPro:IPR037103	EnsemblPlants:KRH17435	ProteinID:KRH17435	ProteinID:KRH17435.1
PFAM:PF00091	PFAM:PF03953	PRINTS:PR01161	PRINTS:PR01163	ScanProsite:PS00227	ScanProsite:PS00228
PANTHER:PTHR11588	PANTHER:PTHR11588:SF116	SMART:SM00864	SMART:SM00865	SUPFAM:SSF52490	SUPFAM:SSF55307
InterPro:Tub_FtsZ_C	InterPro:Tubulin	InterPro:Tubulin/FtsZ_2-layer-sand-dom	InterPro:Tubulin/FtsZ_C_sf	InterPro:Tubulin/FtsZ_GTPase_sf	InterPro:Tubulin_C
InterPro:Tubulin_CS	InterPro:Tubulin_FtsZ_GTPase	UniParc:UPI000233B9C9	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr14:-:48429763..48432990

Molecular Weight (calculated)

51534.5 Da

IEP (calculated)

4.330

GRAVY (calculated)

-0.451

Length

457 amino acids

Sequence
(BLAST)

001: MREILHVQGG QCGNQIGSKF WEVICDEHGI DPTGKYTGDE ASDLQLERIN VYYNEASGGR YVPRAVLMDL EPGTMDSIRS GPYGKIFRPD NFVFGQSGAG
101: NNWAKGHYTE GAELIDSVLD VVRKEAENCD CLQGFQVCHS LGGGTGSGMG TLLISKIREE YPDRMMLTFS VFPSPKVSDT VVEPYNATLS VHQLVENADE
201: CMVLDNEALY DICFRTLKLT TPSFGDLNHL ISATMSGVTC CLRFPGQLNS DLRKLAVNLI PFPRLHFFMV GFAPLTSRGS QQYVSLTVPE LTQQMWDAKN
301: MMCAADPRHG RYLTASAMFR GKMSTKEVDE QMINVQNKNS SYFVEWIPNN VKSSVCDIPP KNLKMSCTFI GNSTSIQEMF RRVSEQFTAM YRRKAFLHWY
401: TGEGMDEMEF TEAESNMNDL VAEYQQYQDA TADEDEYDDE DEYDDEDEDG EHAFEDQ

Best Arabidopsis Sequence Match ( AT1G75780.1 )
(BLAST)

001: MREILHVQGG QCGNQIGSKF WEVICDEHGV DPTGRYNGDS ADLQLERINV YYNEASGGRY VPRAVLMDLE PGTMDSIRSG PYGQIFRPDN FVFGQSGAGN
101: NWAKGHYTEG AELIDAVLDV VRKEAENCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY PDRMMLTFSV FPSPKVSDTV VEPYNATLSV HQLVENADEC
201: MVLDNEALYD ICFRTLKLST PSFGDLNHLI SATMSGVTCS LRFPGQLNSD LRKLAVNLIP FPRLHFFMVG FAPLTSRGSQ QYISLTVPEL TQQMWDAKNM
301: MCAADPRHGR YLTASAMFRG KMSTKEVDEQ ILNVQNKNSS YFVEWIPNNV KSSVCDIPPT GIKMASTFVG NSTSIQEMFR RVSEQFTAMF RRKAFLHWYT
401: GEGMDEMEFT EAESNMNDLV SEYQQYQDAT ADEEDEYDEE EEQVYES

Arabidopsis Description

TUBB1Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot;Acc:P12411]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.