CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 4
nucleus 2
mitochondrion 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH17436
KRH69862

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G20010.1	KRH17436	AT3G50960.1	18390592
AT1G20010.1	KRH69862	AT3G50960.1	18390592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH61035	Soybean	nucleus	98.44	98.22
KRH05958	Soybean	nucleus	96.21	95.99
KRH58404	Soybean	nucleus	93.53	94.8
Bra015815.1-P	Field mustard	cytosol	93.97	94.18
CDX73198	Canola	cytosol	93.97	94.18
CDX87664	Canola	cytosol	93.97	94.18
KRH36829	Soybean	nucleus	93.75	94.17
KRH42279	Soybean	cytosol	93.08	94.13
KRH17435	Soybean	nucleus	95.98	94.09
KRH11812	Soybean	nucleus	93.53	93.95
VIT_18s0001g14360.t01	Wine grape	cytosol	93.75	93.75
AT1G75780.1	Thale cress	cytosol	93.3	93.51
KRH66728	Soybean	nucleus	93.08	92.87
KRG95065	Soybean	plasma membrane	93.08	92.67
Solyc04g081490.2.1	Tomato	nucleus	92.86	92.24
PGSC0003DMT400025739	Potato	cytosol	92.86	92.24
Solyc12g089310.1.1	Tomato	nucleus	92.63	92.02
KRH75786	Soybean	nucleus	92.19	91.98
KRH65977	Soybean	nucleus	91.96	91.76
PGSC0003DMT400075425	Potato	cytosol	92.19	91.57
AT1G20010.1	Thale cress	cytosol	91.74	91.54
KRG91525	Soybean	nucleus	91.07	91.07
KRH35301	Soybean	nucleus	91.07	91.07
KRH41169	Soybean	cytosol	90.18	90.79
KRH59870	Soybean	endoplasmic reticulum	90.18	90.58
KRH41834	Soybean	cytosol	89.51	90.11
KRH60536	Soybean	endoplasmic reticulum	89.29	89.89
KRH04356	Soybean	cytosol	25.89	46.22
KRH58942	Soybean	nucleus	42.41	42.32
KRH42841	Soybean	cytosol, nucleus	42.41	42.22
KRH35640	Soybean	cytosol, nucleus	42.19	42.09
KRG91143	Soybean	nucleus	41.96	41.87
KRH06693	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	41.29	41.76
KRG94842	Soybean	cytosol, endoplasmic reticulum, mitochondrion	41.96	41.69
KRH62139	Soybean	nucleus	41.96	41.59
KRH52851	Soybean	cytosol, nucleus	41.96	41.59
KRH28301	Soybean	cytosol, endoplasmic reticulum	41.74	41.46
KRH58181	Soybean	nucleus	41.52	41.33
KRH77187	Soybean	endoplasmic reticulum	41.52	41.24
KRH08505	Soybean	cytosol, endoplasmic reticulum, nucleus	40.62	40.99
KRH04357	Soybean	cytosol	13.62	35.88
KRH68880	Soybean	endoplasmic reticulum	35.49	33.54
KRG97131	Soybean	cytosol	35.49	33.54

Protein Annotations

Gene3D:1.10.287.600	EntrezGene:100819883	MapMan:20.1.1.2	Gene3D:3.30.1330.20	Gene3D:3.40.50.1440	EMBL:ACUP02003385
InterPro:Beta-tubulin_BS	InterPro:Beta_tubulin	ncoils:Coil	EnsemblPlantsGene:GLYMA_06G024100	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0003924	GO:GO:0005198	GO:GO:0005200	GO:GO:0005488	GO:GO:0005525
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005856	GO:GO:0005874
GO:GO:0007010	GO:GO:0007017	GO:GO:0008150	GO:GO:0009987	GO:GO:0016043	GO:GO:0016787
UniProt:I1K7J4	InterPro:IPR023123	InterPro:IPR036525	InterPro:IPR037103	EnsemblPlants:KRH51701	ProteinID:KRH51701
ProteinID:KRH51701.1	PFAM:PF00091	PFAM:PF03953	PRINTS:PR01161	PRINTS:PR01163	ScanProsite:PS00227
ScanProsite:PS00228	PANTHER:PTHR11588	PANTHER:PTHR11588:SF116	SMART:SM00864	SMART:SM00865	SUPFAM:SSF52490
SUPFAM:SSF55307	InterPro:Tub_FtsZ_C	InterPro:Tubulin	InterPro:Tubulin/FtsZ_2-layer-sand-dom	InterPro:Tubulin/FtsZ_C_sf	InterPro:Tubulin/FtsZ_GTPase_sf
InterPro:Tubulin_C	InterPro:Tubulin_CS	InterPro:Tubulin_FtsZ_GTPase	UniParc:UPI00023395EC	SEG:seg	:

Description

hypothetical protein

Coordinates

chr6:+:1806834..1810627

Molecular Weight (calculated)

50468.5 Da

IEP (calculated)

4.492

GRAVY (calculated)

-0.417

Length

448 amino acids

Sequence
(BLAST)

001: MREILHVQGG QCGNQIGSKF WEVICDEHGI DPTGKYNDEG NSGIQLERIN VYYNEASGGR YVPRAVLMDL EPGTMDSIRS GPYGKIFRPD NFVFGQSGAG
101: NNWAKGHYTE GAELIDSVLD VVRKEAENCD CLQGFQVCHS LGGGTGSGMG TLLISKIREE YPDRMMLTFS VFPSPKVSDT VVEPYNATLS VHQLVENADE
201: CMVLDNEALY DICFRTLKLS TPSFGDLNHL ISATMSGVTC CLRFPGQLNS DLRKLAVNLI PFPRLHFFMV GFAPLTSRGS QQYVSLTVPE LTQQMWDAKN
301: MMCAADPRHG RYLTASAMFR GKMSTKEVDE QMINVQNKNS SYFVEWIPNN VKSSVCDIPP RNLKMSSTFI GNSTSIQEMF RRVSEQFTAM YRRKAFLHWY
401: TGEGMDEMEF TEAESNMNDL VAEYQQYQDA TAEEDDEYED EGEEQYVE

Best Arabidopsis Sequence Match ( AT1G20010.1 )
(BLAST)

001: MREILHIQGG QCGNQIGSKF WEVICDEHGI DSTGRYSGDT ADLQLERINV YYNEASGGRY VPRAVLMDLE PGTMDSIRSG PFGQIFRPDN FVFGQSGAGN
101: NWAKGHYTEG AELIDAVLDV VRKEAENCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY PDRMMLTFSV FPSPKVSDTV VEPYNATLSV HQLVENADEC
201: MVLDNEALYD ICFRTLKLST PSFGDLNHLI SATMSGVTCS LRFPGQLNSD LRKLAVNLIP FPRLHFFMVG FAPLTSRGSQ QYISLTVPEL TQQMWDSKNM
301: MCAADPRHGR YLTASAIFRG QMSTKEVDEQ ILNIQNKNSS YFVEWIPNNV KSSVCDIPPK GLKMAATFVG NSTSIQEMFR RVSEQFTAMF RRKAFLHWYT
401: GEGMDEMEFT EAESNMNDLV AEYQQYQDAT ADEEGEYDVE EEEEGDYET

Arabidopsis Description

TUBB5Tubulin beta-5 chain [Source:UniProtKB/Swiss-Prot;Acc:P29513]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.