Factsheet for Os05t0343400-01

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 6
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 17532485

gfp PMID: 17532485 doi

T Chujo, R Takai, C Akimoto-Tomiyama, S Ando, E Minami, Y Nagamura, H Kaku, N Shibuya, M Yasuda, H Nakashita, K Umemura, A Okada, K Okada, H Nojiri, H Yamane

Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0539500-01
Os04t0417600-00
Os06t0154500-01
Os08t0102900-00
Os10t0533600-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G30250.1	Os06t0154500-01	AT2G43790.1	15990873
AT2G30250.1	Os02t0539500-01	AT3G18690.1	15990873
AT2G30250.1	Os04t0417600-00	AT3G18690.1	15990873
AT2G30250.1	Os08t0102900-00	AT3G18690.1	15990873
AT2G30250.1	Os10t0533600-01	AT4G01370.1	15990873

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS1D01G072900.1	Wheat	nucleus	70.64	74.14
TraesCS1A01G070400.1	Wheat	nucleus	66.12	73.18
Zm00001d038023_P001	Maize	nucleus	72.69	72.39
TraesCS1B01G088900.2	Wheat	nucleus	67.76	72.05
KXG21728	Sorghum	nucleus	76.39	71.95
Zm00001d010399_P001	Maize	nucleus	73.1	71.49
AT5G07100.1	Thale cress	nucleus	35.52	55.99
CDY05016	Canola	nucleus	35.52	54.23
Bra028707.1-P	Field mustard	nucleus	35.32	53.92
CDY14055	Canola	nucleus	34.29	52.35
CDY29685	Canola	nucleus	34.09	47.43
Bra021623.1-P	Field mustard	nucleus	33.88	47.14
CDX97834	Canola	nucleus	34.7	46.81
CDY10720	Canola	nucleus	35.52	46.38
Bra018325.1-P	Field mustard	nucleus	35.52	46.38
CDX84848	Canola	nucleus	34.7	44.71
CDY24017	Canola	nucleus	34.7	44.71
AT2G30250.1	Thale cress	nucleus	35.93	44.53
CDY02051	Canola	nucleus	25.26	44.4
Bra022786.1-P	Field mustard	nucleus	34.09	43.92
Os01t0826400-01	Rice	extracellular	46.82	41.08
KRH00362	Soybean	nucleus	43.12	38.82
KRH40825	Soybean	nucleus	42.09	37.75
KRH65526	Soybean	nucleus	38.6	37.08
Os05t0474800-01	Rice	nucleus	43.33	36.89
KRH76058	Soybean	nucleus	37.99	36.49
Os12t0507300-00	Rice	nucleus	25.26	30.83
Os03t0758950-01	Rice	nucleus, plastid	27.1	30.07
Os07t0596900-00	Rice	nucleus	26.08	29.4
Os07t0583700-01	Rice	nucleus	34.5	27.18
Os08t0499300-01	Rice	nucleus	37.17	26.85
Os03t0444900-00	Rice	endoplasmic reticulum, golgi	19.51	25.47
Os09t0481700-01	Rice	nucleus	28.34	21.8
Os04t0471700-00	Rice	nucleus	38.6	18.84
Os08t0276200-01	Rice	cytosol, nucleus, plastid	12.53	16.67
Os03t0758900-01	Rice	plastid	0.21	0.25

Protein Annotations

MapMan:15.5.22	Gene3D:2.20.25.80	MapMan:26.8.3.3.1	EntrezGene:4338474	ProteinID:AAV44164.1	EMBL:AB190436
ProteinID:BAF17166.1	ProteinID:BAS93479.1	ProteinID:EEE63326.1	GO:GO:0003674	GO:GO:0003676	GO:GO:0003677
GO:GO:0003700	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0006139	GO:GO:0006351	GO:GO:0006355	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058
GO:GO:0009987	GO:GO:0043565	InterPro:IPR003657	InterPro:IPR036576	EnsemblPlantsGene:Os05g0343400	EnsemblPlants:Os05t0343400-01
PFAM:PF03106	PFscan:PS50811	PANTHER:PTHR31221	PANTHER:PTHR31221:SF86	UniProt:Q5W6M7	SMART:SM00774
SUPFAM:SSF118290	UniParc:UPI000003EDF1	InterPro:WRKY_dom	InterPro:WRKY_dom_sf	RefSeq:XP_015640378.1	SEG:seg

Description

WRKY GENE 53WRKY transcription factor, Disease resistance, Defense response (Os05t0343400-01)

Coordinates

chr5:+:16150266..16152633

Molecular Weight (calculated)

50982.9 Da

IEP (calculated)

8.058

GRAVY (calculated)

-0.648

Length

487 amino acids

Sequence
(BLAST)

001: MASSTGGLDH GFTFTPPPFI TSFTELLSGG GGDLLGAGGE ERSPRGFSRG GARVGGGVPK FKSAQPPSLP LSPPPVSPSS YFAIPPGLSP TELLDSPVLL
101: SSSHILASPT TGAIPAQRYD WKASADLIAS QQDDSRGDFS FHTNSDAMAA QPASFPSFKE QEQQVVESSK NGAAAASSNK SGGGGNNKLE DGYNWRKYGQ
201: KQVKGSENPR SYYKCTYNGC SMKKKVERSL ADGRITQIVY KGAHNHPKPL STRRNASSCA TAAACADDLA APGAGADQYS AATPENSSVT FGDDEADNAS
301: HRSEGDEPEA KRWKEDADNE GSSGGMGGGA GGKPVREPRL VVQTLSDIDI LDDGFRWRKY GQKVVKGNPN PRSYYKCTTV GCPVRKHVER ASHDTRAVIT
401: TYEGKHNHDV PVGRGGGGGR APAPAPPTSG AIRPSAVAAA QQGPYTLEML PNPAGLYGGY GAGAGGAAFP RTKDERRDDL FVESLLC

Best Arabidopsis Sequence Match ( AT2G38470.1 )
(BLAST)

001: MAASFLTMDN SRTRQNMNGS ANWSQQSGRT STSSLEDLEI PKFRSFAPSS ISISPSLVSP STCFSPSLFL DSPAFVSSSA NVLASPTTGA LITNVTNQKG
101: INEGDKSNNN NFNLFDFSFH TQSSGVSAPT TTTTTTTTTT TTNSSIFQSQ EQQKKNQSEQ WSQTETRPNN QAVSYNGREQ RKGEDGYNWR KYGQKQVKGS
201: ENPRSYYKCT FPNCPTKKKV ERSLEGQITE IVYKGSHNHP KPQSTRRSSS SSSTFHSAVY NASLDHNRQA SSDQPNSNNS FHQSDSFGMQ QEDNTTSDSV
301: GDDEFEQGSS IVSRDEEDCG SEPEAKRWKG DNETNGGNGG GSKTVREPRI VVQTTSDIDI LDDGYRWRKY GQKVVKGNPN PRSYYKCTTI GCPVRKHVER
401: ASHDMRAVIT TYEGKHNHDV PAARGSGYAT NRAPQDSSSV PIRPAAIAGH SNYTTSSQAP YTLQMLHNNN TNTGPFGYAM NNNNNNSNLQ TQQNFVGGGF
501: SRAKEEPNEE TSFFDSFMP

Arabidopsis Description

WRKY33WRKY33 [Source:UniProtKB/TrEMBL;Acc:A0A178VLS4]
SUBAcon: [plastid,nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.