Factsheet for Zm00001d049522_P001

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, cytosol

Predictor Summary:

cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, plasma membrane
Any Predictor:	cytosol, mitochondrion
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

plasma membrane: 27341663

msms PMID: 27341663 doi

P Voothuluru, JC Anderson, RE Sharp, SC Peck

Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA., Division of Biochemistry, University of Missouri, Columbia, MO, 65211, USA., Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA., Interdisciplinary Plant Group, University of Missouri, Columbia, MO, 65211, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
Zm00001d013996_P003
Zm00001d039404_P001
Zm00001d045530_P001

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G77140.1	Zm00001d013996_P003	AT4G02195.1	10888666
AT1G77140.1	Zm00001d039404_P001	AT4G02195.1	10888666
AT1G77140.1	Zm00001d045530_P001	AT4G02195.1	10888666
AT1G77140.1	Zm00001d013996_P003	AT5G26980.1	10888666
AT1G77140.1	Zm00001d039404_P001	AT5G26980.1	10888666
AT1G77140.1	Zm00001d045530_P001	AT5G26980.1	10888666

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES13788	Sorghum	cytosol	87.2	97.35
TraesCS2D01G259600.1	Wheat	cytosol	82.62	92.24
TraesCS2B01G277800.2	Wheat	cytosol	82.62	92.24
TraesCS2A01G264300.1	Wheat	cytosol	82.62	92.24
Os02t0437800-02	Rice	cytosol, plasma membrane	82.46	92.06
HORVU2Hr1G062800.7	Barley	cytosol, plastid	82.31	82.96
GSMUA_Achr10P...	Banana	cytosol	72.67	81.42
VIT_18s0001g15600.t01	Wine grape	cytosol	71.25	79.4
KRH64544	Soybean	endoplasmic reticulum	70.14	78.17
KRH53395	Soybean	cytosol	70.14	78.17
CDX79189	Canola	cytosol	33.65	77.45
Bra008294.1-P	Field mustard	cytosol	69.35	77.15
CDY38360	Canola	cytosol	69.19	76.98
AT1G77140.1	Thale cress	cytosol	68.88	76.63
Solyc04g064820.2.1	Tomato	cytosol, nucleus	68.72	76.58
CDY70457	Canola	cytosol	42.34	74.86
CDX79215	Canola	cytosol	46.6	71.26
CDY13575	Canola	cytosol	60.82	67.19
VIT_03s0038g00020.t01	Wine grape	cytosol	7.42	64.38
CDX79223	Canola	cytosol, plastid	6.48	62.12
CDX79222	Canola	cytosol, mitochondrion	18.64	43.54
CDY71507	Canola	cytosol, golgi	19.27	41.08
Zm00001d030527_P001	Maize	cytosol, mitochondrion	19.27	19.65
Zm00001d051824_P001	Maize	plasma membrane	19.12	19.27
Solyc10g074520.1.1	Tomato	cytosol	8.21	17.22
Zm00001d015890_P002	Maize	cytosol	18.48	17.21
Zm00001d004159_P003	Maize	cytosol, plastid	18.01	16.96
Zm00001d045434_P004	Maize	cytosol	17.85	16.4
Solyc10g074510.1.1	Tomato	cytosol	1.58	16.39
Zm00001d005839_P001	Maize	cytosol, golgi, plastid	11.85	15.4

Protein Annotations

MapMan:22.7.7.1	Gene3D:3.40.50.2060	UniProt:A0A1D6PVU2	ProteinID:AQK50695.1	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005773	GO:GO:0005794	GO:GO:0005802	GO:GO:0006810
GO:GO:0006886	GO:GO:0006904	GO:GO:0008150	GO:GO:0009705	GO:GO:0009987	GO:GO:0016020
GO:GO:0016192	PFAM:PF00995	PIRSF:PIRSF005715	PANTHER:PTHR11679	PANTHER:PTHR11679:SF3	SUPFAM:SSF56815
InterPro:Sec1-like	InterPro:Sec1-like_sf	UniParc:UPI0008427473	EnsemblPlantsGene:Zm00001d049522	EnsemblPlants:Zm00001d049522_P001	EnsemblPlants:Zm00001d049522_T001
SEG:seg	:	:	:	:	:

Description

Vacuolar protein sorting-associated protein 45 homolog

Coordinates

chr4:-:33126942..33135317

Molecular Weight (calculated)

71773.8 Da

IEP (calculated)

8.282

GRAVY (calculated)

-0.166

Length

633 amino acids

Sequence
(BLAST)

001: MVLITLVRDY IDRMLHDIPG MKVLVLDPQT VRAPSPGRPQ LRLAARSSPI FDLTRSVPSQ VGMVSVVYSQ SDLLRKEVFL VETMDNASSS RESMAHLKAV
101: YFLRPSANNV QKLRRQLAMP RFAEYHLFFS SILKVPQIQI LADSDEQEVV QQVQEFYADF CAIDPYHFTL NIQNNHMYML PTVVDPPGMQ GFCDRAVDGI
201: ASVFLALKRR PVIRYQRTSD VAKRIAQETA RLMYEQESGL FDFRRTENSS LLLVIDRRDD PVTPLLNQWT YQAMVHELIG IENNKVDLMG FANIPKDQQE
301: VVLSSVQDDF FRANMFENFG DLGMNLKRMV DDFQHLSKSS LNLQSIVVLG DMAKFVSNYP EYRKTHGNVT KHVNLVSELS RIVEERKLML VSQTEQELAC
401: TSGQAAAFEA VTSLLNNESV SDIDRLRLVM LYALRYEKES PVQLMQLFNK LASHSAKYKS GSRLFFQGEL VKGTVLMESW LLSLGTRQPN SCVHLVQFLL
501: KQAGIDKRTG DLYGNRDLLN IARNMARGLK GVENVYTQHQ PLLFQTMEGI VKGRLRDADY PLVGNHFQQG RPQDVVIFIV GGTTYEEARS VALYNAANPG
601: VRFFLGGSVV LSSKRFLEDL GEAQRISKSS TII

Best Arabidopsis Sequence Match ( AT1G77140.1 )
(BLAST)

001: MVLVTSVRDY INRMLQDISG MKVLILDSET VSNVSIVYSQ SELLQKEVFL VEMIDSISVS KESMSHLKAV YFIRPTSDNI QKLRYQLANP RFGEYHLFFS
101: NLLKDTQIHI LADSDEQEVV QQVQEYYADF VSGDPYHFTL NMASNHLYMI PAVVDPSGLQ RFSDRVVDGI AAVFLALKRR PVIRYQRTSD TAKRIAHETA
201: KLMYQHESAL FDFRRTESSP LLLVIDRRDD PVTPLLNQWT YQAMVHELIG LQDNKVDLKS IGSLPKDQQV EVVLSSEQDA FFKSNMYENF GDIGMNIKRM
301: VDDFQQVAKS NQNIQTVEDM ARFVDNYPEY KKMQGNVSKH VTLVTEMSKL VEARKLMTVS QTEQDLACNG GQGAAYEAVT DLLNNESVSD IDRLRLVMLY
401: ALRYEKENPV QLMQLFNKLA SRSPKYKPGL VQFLLKQAGV EKRTGDLFGN RDLLNIARNM ARGLKGVENV YTQHQPLLFQ TMESITRGRL RDVDYPFVGD
501: HFQQGRPQEV VIFMVGGTTY EESRSVALQN ATNSGVRFIL GGTAVLNSKR FLKDLEEAQR ISRSGSHMV

Arabidopsis Description

VPS45VPS45 [Source:UniProtKB/TrEMBL;Acc:A0A178WPE4]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.