CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

extracellular 2
endoplasmic reticulum 2
vacuole 3
plasma membrane 2
golgi 2
cytosol 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, nucleus
Any Predictor:	cytosol, secretory
BaCelLo:	secretory
EpiLoc:	vacuole
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	secretory
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH06401
KRH47835

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G37600.1	KRH06401	AT2G46700.1	16472779
AT5G37600.1	KRH47835	AT2G46700.1	16472779

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH48674	Soybean	endoplasmic reticulum	98.31	98.31
KRG97948	Soybean	cytosol, endoplasmic reticulum, nucleus	90.17	90.17
KRH30929	Soybean	endoplasmic reticulum	89.33	89.33
Solyc04g014510.2.1	Tomato	cytosol, extracellular, plastid, unclear	88.76	88.76
CDX74573	Canola	cytosol	88.76	88.76
KXG30808	Sorghum	cytosol	88.48	88.48
PGSC0003DMT400060733	Potato	cytosol	88.48	88.48
KRH17323	Soybean	cytosol	88.48	88.48
VIT_01s0011g02200.t01	Wine grape	cytosol	88.48	88.48
Zm00001d051804_P001	Maize	cytosol, extracellular, mitochondrion, plasma membrane	88.2	88.2
CDY01727	Canola	cytosol	86.24	87.97
KRH72976	Soybean	cytosol	87.92	87.92
Bra028132.1-P	Field mustard	cytosol	87.92	87.92
CDY52941	Canola	cytosol	87.64	87.64
CDY16439	Canola	cytosol	87.64	87.64
AT5G37600.1	Thale cress	cytosol	87.64	87.64
Bra039756.1-P	Field mustard	cytosol	87.36	87.36
Os02t0735200-02	Rice	cytosol	87.08	87.08
CDX85499	Canola	cytosol	86.24	86.24
Bra023573.1-P	Field mustard	cytosol	86.24	86.24
CDY38104	Canola	cytosol	85.96	85.96
CDY49656	Canola	cytosol	85.67	85.67
Bra008612.1-P	Field mustard	cytosol	85.67	85.67
TraesCS6A01G298100.2	Wheat	cytosol	85.39	85.39
CDX69442	Canola	cytosol	85.39	85.39
AT1G66200.3	Thale cress	cytosol	87.36	85.21
TraesCS6B01G327500.1	Wheat	cytosol, golgi, plastid, unclear	85.11	85.11
AT5G16570.1	Thale cress	cytosol	84.83	84.83
Zm00001d017958_P002	Maize	cytosol	86.8	83.97
KRH12677	Soybean	extracellular	26.69	76.61
HORVU6Hr1G074030.2	Barley	cytosol, mitochondrion, plastid	86.24	75.62
KRH72975	Soybean	cytosol	53.65	75.49
KRH20936	Soybean	plastid	78.65	64.81
KRH11349	Soybean	plastid	78.65	64.81
KRH17322	Soybean	cytosol	45.51	64.29
KRH71070	Soybean	extracellular	16.85	62.5
KRH25519	Soybean	plastid	20.22	37.89

Protein Annotations

KEGG:00220+6.3.1.2	KEGG:00250+6.3.1.2	KEGG:00630+6.3.1.2	KEGG:00910+6.3.1.2	MapMan:25.1.5.1.1	Gene3D:3.10.20.70
Gene3D:3.30.590.40	EntrezGene:547620	EMBL:ACUP02005906	EMBL:BT093748	UniProt:C6T8F0	EnsemblPlantsGene:GLYMA_09G173200
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004356	GO:GO:0005488	GO:GO:0005524
GO:GO:0006542	GO:GO:0006807	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987
GO:GO:0016874	InterPro:Gln_synt_N	InterPro:Gln_synt_b-grasp	InterPro:Gln_synth/guanido_kin_cat_dom	InterPro:Gln_synth_N_conserv_site	InterPro:Gln_synth_cat_dom
InterPro:Gln_synth_gly_rich_site	InterPro:IPR036651	EnsemblPlants:KRH39031	ProteinID:KRH39031	ProteinID:KRH39031.1	PFAM:PF00120
PFAM:PF03951	ScanProsite:PS00180	ScanProsite:PS00181	PANTHER:PTHR20852	PANTHER:PTHR20852:SF66	MetaCyc:PWY-381
MetaCyc:PWY-5675	MetaCyc:PWY-6549	MetaCyc:PWY-6963	MetaCyc:PWY-6964	SMART:SM01230	SUPFAM:SSF54368
SUPFAM:SSF55931	UniParc:UPI00019D33FC	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr9:+:39770478..39774858

Molecular Weight (calculated)

39214.1 Da

IEP (calculated)

5.024

GRAVY (calculated)

-0.467

Length

356 amino acids

Sequence
(BLAST)

001: MSLLSDLINL NLSESTEKIV AEYIWVGGSG MDLRSKARTL PGPVSDPAKL PKWNYDGSST DQAPGDDSEV ILYPQAIFKD PFRRGNNILV ICDVYTPAGE
101: PLPTNKRYDA AKIFSHPDVA AEEPWYGIEQ EYTLLQKDVN WPLGWPLGGF PGPQGPYYCG TGADKAYGRD IVDAHYKACI YAGINISGIN GEVMPGQWEF
201: QVGPSVGISA GDEVWAARYI LERITEMAGV IVSFDPKPIP GDWNGAGAHS NYSTKSMRDE GGYEVIKKAI EKLGLRHKEH IAAYGEGNER RLTGRHETAD
301: INTFSWGVAN RGSSIRVGRD TEKNGKGYFE DRRPASNMDP YVVTSMIAET TILWKP

Best Arabidopsis Sequence Match ( AT5G37600.1 )
(BLAST)

001: MSLVSDLINL NLSDSTDKII AEYIWVGGSG MDMRSKARTL PGPVTDPSQL PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE
101: PIPTNKRHAA AKVFSNPDVA AEVPWYGIEQ EYTLLQKDVK WPVGWPIGGY PGPQGPYYCG IGADKSFGRD VVDSHYKACL YAGINISGIN GEVMPGQWEF
201: QVGPAVGISA ADEIWVARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHC NYSTKSMREE GGYEIIKKAI DKLGLRHKEH IAAYGEGNER RLTGHHETAD
301: INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YIVTSMIAET TILWNP

Arabidopsis Description

GLN1-1Glutamine synthetase cytosolic isozyme 1-1 [Source:UniProtKB/Swiss-Prot;Acc:Q56WN1]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.