CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 3
plastid 4
cytosol 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG95941
KRH19825
KRH32420
KRH44616
KRH67648

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G10840.1	KRH44616	AT3G21175.1	21798944
AT4G10840.1	KRG95941	AT5G03040.1	21798944
AT4G10840.1	KRH19825	AT5G03040.1	21798944
AT4G10840.1	KRH32420	AT5G03040.1	21798944
AT4G10840.1	KRH67648	AT5G03040.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH60081	Soybean	nucleus	91.18	93.61
Solyc08g008460.2.1	Tomato	cytosol, nucleus	56.75	82.41
VIT_02s0025g02090.t01	Wine grape	plastid	80.45	78.68
CDX94243	Canola	cytosol	37.02	78.39
CDY05122	Canola	plastid	79.76	75.95
CDX90788	Canola	plastid	79.41	75.74
AT4G10840.1	Thale cress	plastid	79.41	75.37
Bra000724.1-P	Field mustard	plastid	79.24	75.33
PGSC0003DMT400031631	Potato	plastid	77.34	74.38
PGSC0003DMT400014982	Potato	plastid	77.34	73.88
KRH06445	Soybean	cytosol	67.99	70.94
TraesCS6D01G030400.1	Wheat	cytosol	60.03	70.24
HORVU6Hr1G004990.1	Barley	cytosol	62.63	68.43
GSMUA_Achr10P...	Banana	cytosol	68.86	68.15
Zm00001d054069_P001	Maize	plasma membrane	68.51	66.67
Os02t0109800-01	Rice	plasma membrane	67.82	65.44
TraesCS6B01G037200.1	Wheat	plastid	67.99	64.53
EES06120	Sorghum	plastid	67.13	63.4
Zm00001d014961_P001	Maize	extracellular, mitochondrion, plasma membrane	66.44	62.75
TraesCS6A01G026600.1	Wheat	plastid	67.99	57.46
KRG93305	Soybean	cytosol	59.69	49.29
KRH56614	Soybean	cytosol	57.96	47.72
KRH63534	Soybean	nucleus	58.13	46.28
KRH03299	Soybean	cytosol	31.49	45.61
KRH57064	Soybean	cytosol	57.44	45.48
KRH54405	Soybean	cytosol	57.27	43.96
KRH32172	Soybean	mitochondrion	26.3	24.72
KRH19545	Soybean	mitochondrion	25.26	23.7

Protein Annotations

Gene3D:1.25.40.10	EntrezGene:100815100	MapMan:21.1.1.4.3	EMBL:ACUP02004761	ncoils:Coil	EnsemblPlantsGene:GLYMA_08G025300
GO:GO:0003674	GO:GO:0003824	GO:GO:0004553	GO:GO:0005488	GO:GO:0005515	GO:GO:0005575
GO:GO:0005623	GO:GO:0016787	GO:GO:0042597	UniProt:I1KPM9	InterPro:IPR011990	InterPro:IPR013026
InterPro:IPR019734	EnsemblPlants:KRH41360	ProteinID:KRH41360	ProteinID:KRH41360.1	PFAM:PF13374	PFAM:PF13424
PFscan:PS50005	PFscan:PS50293	PANTHER:PTHR19959	PANTHER:PTHR19959:SF202	SMART:SM00028	SUPFAM:SSF48452
InterPro:TPR-contain_dom	InterPro:TPR-like_helical_dom_sf	InterPro:TPR_repeat	UniParc:UPI0002339231	SEG:seg	:

Description

hypothetical protein

Coordinates

chr8:-:2001482..2005405

Molecular Weight (calculated)

63284.6 Da

IEP (calculated)

5.061

GRAVY (calculated)

-0.306

Length

578 amino acids

Sequence
(BLAST)

001: MPGLVSDAQP LGISVPDTPR QSQRTPSPLP KKPPSPSPSR SSKKKTPETP PLLSDASLDN PDLGPFLLKL ARDTIASGDG PSKALDFAIR ASKSFERCAV
101: EGEPSLDLAM SLHVLAAIYC SLGRFDEAVP VLERAIQVPD VERGADHALA AFSGYMQLGD TFSMLGQVDR SISCYDQGLQ IQIQALGDSD PRVGETCRYL
201: AEANVQAMQF DKAEDLCKKT LEIHRAHSEP ASLEEAADRR LMALICEAKG DYESALEHLV LASMAMIANG QDNEVASIDV SIGNIYMSLC RFDEAIFSYQ
301: KALTVFKSAK GENHPSVASV FVRLADLYHR TGKLRESKSY CENALRIYSK PVPGTTAEEI AGGLTEISAV FESVDEPEEA LKLLNRAMKL LEDKPGQQST
401: IAGIEARMGV MYYMIGRYED SRNSFESAVA KLRASGERKS AFFGVVLNQM GLACVQLFKI DEAAELFEEA RGILEQECGP CHQDTLGVYS NLAATYDAMG
501: RVGDAIEILE YVLKLREEKL GIANPDFEDE KRRLAELLKE AGKTRDRKAK SLENLIDPGS KRTKKEGAKR WPGLGFRI

Best Arabidopsis Sequence Match ( AT4G10840.1 )
(BLAST)

001: MPAMPGLVSV KTPSDTPPLR VAVPDTQPLS NPPRTPMKKT PSSTPSRSKP SPNRSTGKKD SPTVSSSTAA VIDVDDPSLD NPDLGPFLLK LARDAIASGE
101: GPNKALDYAI RATKSFERCC AAVAPPIPGG SDGGPVLDLA MSLHVLAAIY CSLGRFDEAV PPLERAIQVP DPTRGPDHSL AAFSGHMQLG DTLSMLGQID
201: RSIACYEEGL KIQIQTLGDT DPRVGETCRY LAEAYVQAMQ FNKAEELCKK TLEIHRAHSE PASLEEAADR RLMAIICEAK GDYENALEHL VLASMAMIAS
301: GQESEVASID VSIGNIYMSL CRFDEAVFSY QKALTVFKAS KGETHPTVAS VFVRLAELYH RTGKLRESKS YCENALRIYN KPVPGTTVEE IAGGLTEISA
401: IYESVDEPEE ALKLLQKSMK LLEDKPGQQS AIAGLEARMG VMYYTVGRYE DARNAFESAV TKLRAAGEKS AFFGVVLNQM GLACVQLFKI DEAGELFEEA
501: RGILEQERGP CDQDTLGVYS NLAATYDAMG RIEDAIEILE QVLKLREEKL GTANPDFEDE KKRLAELLKE AGRSRNYKAK SLQNLIDPNA RPPKKESSAK
601: KWPSLGFKF

Arabidopsis Description

KLCR1Protein KINESIN LIGHT CHAIN-RELATED 1 [Source:UniProtKB/Swiss-Prot;Acc:O81629]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.