Factsheet for Os12t0182200-01

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1
mitochondrion 3
plastid 6

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plastid
Any Predictor:	mitochondrion, plastid, secretory
BaCelLo:	secretory
EpiLoc:	mitochondrion
iPSORT:	plastid
MultiLoc:	plastid
Plant-mPloc:	mitochondrion
Predotar:	plastid
PProwler:	plastid
TargetP:	plastid
WoLF PSORT:	plastid
YLoc:	mitochondrion

plastid: 17189339
nucleus: 19621931
plastid: 21433289
extracellular: 24083427
plasma membrane: 28056797

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

msms PMID: 21433289 doi

S Reiland, J Grossmann, K Baerenfaller, P Gehrig, A Nunes-Nesi, AR Fernie, W Gruissem, S Baginsky

Department of Biology, Plant Biotechnology, ETH Zurich, Zurich, Switzerland.

msms PMID: 24083427 doi

T Shinano, T Yoshimura, T Watanabe, Y Unno, M Osaki, Y Nanjo, S Komatsu

Agricultural Radiation Research Center, NARO Tohoku Agricultural Research Center , 50, Harajyukuminami, Arai, Fukushima 960-2156, Japan.

msms PMID: 17189339 doi

T Kleffmann, A von Zychlinski, D Russenberger, M Hirsch-Hoffmann, P Gehrig, W Gruissem, S Baginsky

Institute of Plant Sciences, Eidgenössische Technische Hochschule Zurich, 8092 Zurich, Switzerland.

msms PMID: 19621931 doi

T Aki, S Yanagisawa

Department of Applied Biological Chemistry, The University of Tokyo, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0303000-02
Os03t0306800-01
Os03t0681700-01
Os04t0589200-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G34430.1	Os01t0303000-02	AT2G47400.1	21798944
AT1G34430.1	Os03t0306800-01	AT2G47400.1	21798944
AT1G34430.1	Os04t0589200-01	AT3G17668.1	21798944
AT1G34430.1	Os03t0681700-01	AT3G18140.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS5D01G126000.1	Wheat	cytosol	77.94	89.22
OQU78843	Sorghum	plastid	86.51	88.21
Zm00001d023606_P005	Maize	plastid	85.87	87.75
TraesCS5B01G116300.1	Wheat	golgi, unclear	85.22	86.71
TraesCS5A01G119600.1	Wheat	plastid	83.94	85.96
KRG91819	Soybean	endoplasmic reticulum, nucleus	73.23	73.55
KRH34962	Soybean	plastid	73.45	73.13
Solyc11g017250.1.1	Tomato	plastid	71.31	71.15
VIT_18s0072g01000.t01	Wine grape	plastid	72.38	70.86
PGSC0003DMT400023831	Potato	plastid	70.24	70.69
AT1G34430.1	Thale cress	plastid	68.74	69.03
HORVU5Hr1G038090.3	Barley	plastid	62.1	67.29
Zm00001d041839_P001	Maize	plastid	84.15	66.27
Os08t0431300-01	Rice	plastid	66.17	65.05
GSMUA_Achr3P17420_001	Banana	cytosol	32.33	60.64
Os09t0408600-01	Rice	plastid	61.46	57.29
Os02t0105200-01	Rice	mitochondrion	30.62	26.09
Os07t0410100-01	Rice	mitochondrion	29.55	25.51
Os06t0105400-02	Rice	mitochondrion	29.98	25.45
Os06t0499900-01	Rice	mitochondrion	25.91	25.0
Os02t0514700-00	Rice	mitochondrion	17.56	20.6
Os04t0394200-01	Rice	mitochondrion	19.06	20.23
Os01t0314100-01	Rice	mitochondrion	22.27	19.89
CDY34925	Canola	mitochondrion	10.28	18.68
Bra040295.1-P	Field mustard	mitochondrion	10.28	18.68
TraesCS5D01G125900.1	Wheat	plastid	2.57	3.93
Os01t0314000-01	Rice	cytosol	0.21	0.19
CDY69187	Canola	cytosol, nucleus	0.0	0.0
CDY17299	Canola	cytosol, plastid	0.0	0.0

Protein Annotations

InterPro:2-oxoA_DH_lipoyl-BS	InterPro:2-oxoacid_DH_actylTfrase	Gene3D:2.40.50.100	Gene3D:3.30.559.10	Gene3D:4.10.320.10	EntrezGene:4351678
MapMan:5.1.2.2.2	ProteinID:ABA95959.2	ProteinID:BAF29338.2	ProteinID:BAT16156.1	InterPro:Biotin_lipoyl	InterPro:CAT-like_dom_sf
InterPro:E3-bd_dom_sf	GO:GO:0003674	GO:GO:0003824	GO:GO:0008150	GO:GO:0008152	GO:GO:0016740
GO:GO:0016746	InterPro:IPR000089	InterPro:IPR004167	InterPro:IPR023213	InterPro:IPR036625	EnsemblPlantsGene:Os12g0182200
EnsemblPlants:Os12t0182200-01	PFAM:PF00198	PFAM:PF00364	PFAM:PF02817	ScanProsite:PS00189	PFscan:PS50968
PFscan:PS51826	InterPro:PSBD	PANTHER:PTHR23151	PANTHER:PTHR23151:SF61	UniProt:Q2QWU7	SUPFAM:SSF47005
SUPFAM:SSF51230	SUPFAM:SSF52777	InterPro:Single_hybrid_motif	UniParc:UPI0000D89C5B	RefSeq:XP_015618112.1	SEG:seg

Description

Similar to Dihydrolipoamide S-acetyltransferase. (Os12t0182200-01);Similar to Dihydrolipoyllysine-residue acetyltransferase component of pyruvatedehydrogenase complex. (Os12t0182200-02)

Coordinates

chr12:-:4171993..4176907

Molecular Weight (calculated)

48363.1 Da

IEP (calculated)

9.227

GRAVY (calculated)

0.015

Length

467 amino acids

Sequence
(BLAST)

001: MAALLHLQSS ATAAAAPSFL LLRRRGGGVT APAGSSRRRR ACFRVEAKIR EIFMPALSST MTEGKIVSWT ASEGDRLAKG DPVVVVESDK ADMDVETFHD
101: GFLAAVLVPA GESAPVGSAI ALLAESEDEI PAAQSKAASL SSSSSSSPPP PPPQESTPPP PPPPPPAPVA AAVSAPAPPS PASQGGLRVV ASPYARKLAK
201: DLNVDLNSIT GSGPGGRIVA KDVEAAAAAP KKAAPVAAAR PDVPLGSTVP FTTMQGAVSK NMVESLAVPT FRVGYTFTTD ALDALYKKIK PKGVTMSALL
301: AKATAMALVQ HPVINSSCRD GKSFTYNSSI NIAVAVAIDG GLITPVLPDA DKLDIYSLSR KWKELVDKAR AKQLQPHEYN SGTFTISNLG MFGVDRFDAI
401: LPPGTGAIMA VGSSQPTLVG TKDGSIGIKN QMQVNVTADH RVIYGADLAA FLQTLSKIIE DPKDLTF

Best Arabidopsis Sequence Match ( AT1G34430.1 )
(BLAST)

001: MSRLLQTPFL PSVSLPTKTR SSVTGFRVKP RIIPIQAKIR EIFMPALSST MTEGKIVSWV KSEGDKLNKG ESVVVVESDK ADMDVETFYD GYLAAIMVEE
101: GGVAPVGSAI ALLAETEDEI ADAKAKASGG GGGGDSKAPP ASPPTAAVEA PVSVEKKVAA APVSIKAVAA SAVHPASEGG KRIVASPYAK KLAKELKVEL
201: AGLVGSGPMG RIVAKDVEAV AAGGGVQAAV AVKEVVAAPG VELGSVVPFT TMQGAVSRNM VESLGVPTFR VGYTISTDAL DALYKKIKSK GVTMTALLAK
301: ATALALAKHP VVNSSCRDGN SFVYNSSINV AVAVAIDGGL ITPVLQNADK VDIYSLSRKW KELVDKARAK QLQPQEYNTG TFTLSNLGMF GVDRFDAILP
401: PGTGAIMAVG ASQPSVVATK DGRIGMKNQM QVNVTADHRV IYGADLAQFL QTLASIIEDP KDLTF

Arabidopsis Description

EMB3003Dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q9C8P0]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.