CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 8
mitochondrion 3

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG96526
KRH11050
KRH21226
KRH31794
KRH68260
KRH69219
KRH73492

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G34430.1	KRG96526	AT2G47400.1	21798944
AT1G34430.1	KRH31794	AT2G47400.1	21798944
AT1G34430.1	KRH68260	AT2G47400.1	21798944
AT1G34430.1	KRH69219	AT2G47400.1	21798944
AT1G34430.1	KRH73492	AT3G17668.1	21798944
AT1G34430.1	KRH11050	AT3G18140.1	21798944
AT1G34430.1	KRH21226	AT3G18140.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH34962	Soybean	plastid	97.2	96.38
VIT_18s0072g01000.t01	Wine grape	plastid	81.94	79.87
CDY03145	Canola	plastid	77.42	78.95
CDY12039	Canola	plastid	76.34	78.89
Bra028057.1-P	Field mustard	plastid	76.56	78.24
AT1G34430.1	Thale cress	plastid	77.85	77.85
TraesCS5D01G126000.1	Wheat	cytosol	66.67	75.98
Solyc11g017250.1.1	Tomato	plastid	76.34	75.85
PGSC0003DMT400023831	Potato	plastid	74.84	75.0
Zm00001d023606_P005	Maize	plastid	73.12	74.4
OQU78843	Sorghum	plastid	72.47	73.58
Os12t0182200-01	Rice	plastid	73.55	73.23
TraesCS5A01G119600.1	Wheat	plastid	71.18	72.59
TraesCS5B01G116300.1	Wheat	golgi, unclear	71.61	72.55
GSMUA_Achr3P17420_001	Banana	cytosol	34.41	64.26
KRH75381	Soybean	plastid	55.7	60.66
KRG90801	Soybean	endoplasmic reticulum, nucleus	61.94	56.92
Zm00001d041839_P001	Maize	plastid	71.61	56.16
HORVU5Hr1G038090.3	Barley	plastid	51.4	55.45
KRG92074	Soybean	cytosol, mitochondrion	9.03	38.18
KRH06095	Soybean	mitochondrion	32.69	27.79
KRH47526	Soybean	mitochondrion	32.47	27.61
KRH34708	Soybean	mitochondrion	29.25	20.12
KRH02272	Soybean	mitochondrion	21.94	19.81
KRH50815	Soybean	mitochondrion	21.51	19.42
KRH14369	Soybean	nucleus	19.35	19.4
KRH73773	Soybean	mitochondrion	19.14	19.18
KRG92075	Soybean	mitochondrion	20.65	18.53
TraesCS5D01G125900.1	Wheat	plastid	2.37	3.61

Protein Annotations

EntrezGene:100799926	InterPro:2-oxoA_DH_lipoyl-BS	InterPro:2-oxoacid_DH_actylTfrase	Gene3D:2.40.50.100	Gene3D:3.30.559.10	Gene3D:4.10.320.10
MapMan:5.1.2.2.2	EMBL:ACUP02012708	InterPro:Biotin_lipoyl	InterPro:CAT-like_dom_sf	InterPro:E3-bd_dom_sf	EnsemblPlantsGene:GLYMA_20G176300
GO:GO:0003674	GO:GO:0003824	GO:GO:0008150	GO:GO:0008152	GO:GO:0016740	GO:GO:0016746
UniProt:I1NHB5	InterPro:IPR000089	InterPro:IPR004167	InterPro:IPR023213	InterPro:IPR036625	EnsemblPlants:KRG91819
ProteinID:KRG91819	ProteinID:KRG91819.1	PFAM:PF00198	PFAM:PF00364	PFAM:PF02817	ScanProsite:PS00189
PFscan:PS50968	PFscan:PS51826	InterPro:PSBD	PANTHER:PTHR23151	PANTHER:PTHR23151:SF61	SUPFAM:SSF47005
SUPFAM:SSF51230	SUPFAM:SSF52777	InterPro:Single_hybrid_motif	UniParc:UPI000233D9BB	SEG:seg	:

Description

hypothetical protein

Coordinates

chr20:-:41355494..41361261

Molecular Weight (calculated)

48442.4 Da

IEP (calculated)

9.107

GRAVY (calculated)

0.094

Length

465 amino acids

Sequence
(BLAST)

001: MMAHLLHTPF IPSSSALRRA APIPHRKTTT PLVVRAKIRE IFMPALSSTM TEGKIVSWTK SEGDKLSKGD SVVVVESDKA DMDVETFYDG YLAAIVVEEG
101: GVAAVGSPIA FLAETEDEIP QAKSKASSSS SSSSAPAPAT APAPAPPVES QPEKVAAPVA AAVAAPVVAS SHPASEGGKR IVASPYAKKL AKELKVELGR
201: IVGTGPMGRI VAKDVEAFAA AGNVAAAVAP ALGKSAAPAG VELGSVVPFT TMQSAVSRNM AESLAVPTFR VGYTITTDAL DALYKKIKSK GVTMTALLAK
301: ATALALVKHP VMNSSCRDGN SFTYNSSINI AVAVAIDGGL ITPVLQGADK IDVYSLSRKW KELVDKARAK QLQPHEYNTG TFTLSNLGMF GVDRFDAILP
401: PGTGAIMAVG ASEPTVVATK DGRIGMKNQM QVNVTADHRV IYGADLASFL QTLSQIIEDP KDLTF

Best Arabidopsis Sequence Match ( AT1G34430.1 )
(BLAST)

001: MSRLLQTPFL PSVSLPTKTR SSVTGFRVKP RIIPIQAKIR EIFMPALSST MTEGKIVSWV KSEGDKLNKG ESVVVVESDK ADMDVETFYD GYLAAIMVEE
101: GGVAPVGSAI ALLAETEDEI ADAKAKASGG GGGGDSKAPP ASPPTAAVEA PVSVEKKVAA APVSIKAVAA SAVHPASEGG KRIVASPYAK KLAKELKVEL
201: AGLVGSGPMG RIVAKDVEAV AAGGGVQAAV AVKEVVAAPG VELGSVVPFT TMQGAVSRNM VESLGVPTFR VGYTISTDAL DALYKKIKSK GVTMTALLAK
301: ATALALAKHP VVNSSCRDGN SFVYNSSINV AVAVAIDGGL ITPVLQNADK VDIYSLSRKW KELVDKARAK QLQPQEYNTG TFTLSNLGMF GVDRFDAILP
401: PGTGAIMAVG ASQPSVVATK DGRIGMKNQM QVNVTADHRV IYGADLAQFL QTLASIIEDP KDLTF

Arabidopsis Description

EMB3003Dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q9C8P0]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.