CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 9
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91819
KRH13655
KRH34962
KRH43808
KRH71066

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G25860.1	KRG91819	AT1G34430.1	21798944
AT3G25860.1	KRH34962	AT1G34430.1	21798944
AT3G25860.1	KRH13655	AT1G79070.1	21798944
AT3G25860.1	KRH43808	AT1G79070.1	21798944
AT3G25860.1	KRH71066	AT3G25545.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
GSMUA_Achr3P07350_001	Banana	cytosol	62.65	77.89
KRH75381	Soybean	plastid	64.43	76.35
VIT_01s0011g03380.t01	Wine grape	plastid	72.53	75.2
Solyc05g009530.2.1	Tomato	plastid	67.19	74.07
Bra025167.1-P	Field mustard	plastid	69.57	73.79
PGSC0003DMT400033754	Potato	plastid	67.0	73.7
CDY13593	Canola	plastid	69.37	73.58
CDX83563	Canola	plastid	69.17	73.53
CDY39183	Canola	plastid	68.58	73.52
AT3G25860.1	Thale cress	plastid	69.57	73.33
Bra017345.1-P	Field mustard	plastid	69.37	73.28
CDY04184	Canola	plastid	69.17	73.07
TraesCS5A01G476700.1	Wheat	plastid	64.82	69.2
TraesCS5B01G489600.1	Wheat	golgi	64.43	68.63
Zm00001d032224_P001	Maize	plastid	63.83	68.58
EES15031	Sorghum	plastid	64.03	68.21
TraesCS5D01G490000.1	Wheat	golgi, plastid, unclear	63.83	68.14
Os08t0431300-01	Rice	plastid	63.83	68.0
HORVU5Hr1G061140.1	Barley	plastid	61.46	67.17
TraesCS5D01G212800.1	Wheat	plastid	61.07	66.74
OQU89472	Sorghum	plastid	60.47	66.67
TraesCS3B01G372900.1	Wheat	plastid	59.68	66.52
TraesCS5B01G204800.1	Wheat	plastid	60.08	65.66
Zm00001d020426_P001	Maize	plastid	58.89	65.64
Os09t0408600-01	Rice	plastid	61.66	62.28
KRG91819	Soybean	endoplasmic reticulum, nucleus	56.92	61.94
Zm00001d050383_P001	Maize	plastid	63.64	61.69
KRH34962	Soybean	plastid	56.72	61.19
TraesCS5A01G206600.2	Wheat	plastid	55.14	60.13
HORVU5Hr1G112850.3	Barley	plastid	61.66	58.21
KRG92074	Soybean	cytosol, mitochondrion	8.7	40.0
KRH47526	Soybean	mitochondrion	28.46	26.33
KRH06095	Soybean	mitochondrion	28.46	26.33
KRH50815	Soybean	mitochondrion	22.13	21.75
KRH02272	Soybean	mitochondrion	21.74	21.36
KRH73773	Soybean	mitochondrion	18.58	20.26
KRH14369	Soybean	nucleus	18.58	20.26
KRH34708	Soybean	mitochondrion	26.48	19.82
KRG92075	Soybean	mitochondrion	18.18	17.76

Protein Annotations

EntrezGene:100813087	InterPro:2-oxoA_DH_lipoyl-BS	InterPro:2-oxoacid_DH_actylTfrase	Gene3D:2.40.50.100	Gene3D:3.30.559.10	Gene3D:4.10.320.10
MapMan:5.1.2.2.2	EMBL:ACUP02012641	InterPro:Biotin_lipoyl	InterPro:CAT-like_dom_sf	InterPro:E3-bd_dom_sf	EnsemblPlantsGene:GLYMA_20G114500
GO:GO:0003674	GO:GO:0003824	GO:GO:0008150	GO:GO:0008152	GO:GO:0016740	GO:GO:0016746
UniProt:I1NFH4	InterPro:IPR000089	InterPro:IPR004167	InterPro:IPR023213	InterPro:IPR036625	EnsemblPlants:KRG90801
ProteinID:KRG90801	ProteinID:KRG90801.1	PFAM:PF00198	PFAM:PF00364	PFAM:PF02817	ScanProsite:PS00189
PFscan:PS50968	PFscan:PS51826	InterPro:PSBD	PANTHER:PTHR23151	PANTHER:PTHR23151:SF75	SUPFAM:SSF47005
SUPFAM:SSF51230	SUPFAM:SSF52777	InterPro:Single_hybrid_motif	UniParc:UPI00023DCBA3	SEG:seg	:

Description

hypothetical protein

Coordinates

chr20:+:35619071..35625785

Molecular Weight (calculated)

52702.7 Da

IEP (calculated)

8.342

GRAVY (calculated)

0.106

Length

506 amino acids

Sequence
(BLAST)

001: MSMASLPNSP ALSFSSSLST TANLLPCRRF TAFPRRRASS FSVQAKIREI FMPALSSTMT EGKIVSWIKS EGDMLSKGDS VVVVESDKAD MDVETFYDGI
101: LAAIVVADGE TAPVGAPIGL LADSPEEVAE AKAKAAKSVP SGSPAPASPS DPSPATPPPP PPPAKSVSEG PAKIVATPQA KKLAKQHKVD IATVAGTGPF
201: GRITPADVEA AAGIAPSKRN VAPAAIPAPA TPAPPSKAAS GSAAPAPIPG SSVVPFTTMQ SAVAKNMMES LSVPTFRVGY PVTTDALDAL YEKVKPKGVT
301: MTAILAKAAA MALVQHPVVN ATCKDGKNFV YNGNINVAVA VAINGGLITP VLQDADKLDL YLLSQKWKEL VEKARAKQLQ PHEYNSGVFM NCDCCLLLAK
401: KNFVELGCFN FDPNLIVPAT GTFTLSNLGM FGVDRFDAIL PPGQGAIMAV GASKPTVLAD KDGFFSVKNK MLVNVTADHR IIYGADLAAF LQTFSKIIEN
501: PESLTL

Best Arabidopsis Sequence Match ( AT3G25860.1 )
(BLAST)

001: MAVSSSSFLS TASLTNSKSN ISFASSVSPS LRSVVFRSTT PATSHRRSMT VRSKIREIFM PALSSTMTEG KIVSWIKTEG EKLAKGESVV VVESDKADMD
101: VETFYDGYLA AIVVGEGETA PVGAAIGLLA ETEAEIEEAK SKAASKSSSS VAEAVVPSPP PVTSSPAPAI AQPAPVTAVS DGPRKTVATP YAKKLAKQHK
201: VDIESVAGTG PFGRITASDV ETAAGIAPSK SSIAPPPPPP PPVTAKATTT NLPPLLPDSS IVPFTAMQSA VSKNMIESLS VPTFRVGYPV NTDALDALYE
301: KVKPKGVTMT ALLAKAAGMA LAQHPVVNAS CKDGKSFSYN SSINIAVAVA INGGLITPVL QDADKLDLYL LSQKWKELVG KARSKQLQPH EYNSGTFTLS
401: NLGMFGVDRF DAILPPGQGA IMAVGASKPT VVADKDGFFS VKNTMLVNVT ADHRIVYGAD LAAFLQTFAK IIENPDSLTL

Arabidopsis Description

LTA2Dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q9SQI8]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.