Factsheet for Bra032749.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
mitochondrion 3

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion
BaCelLo:	cytosol
EpiLoc:	mitochondrion
MultiLoc:	cytosol
Plant-mPloc:	mitochondrion
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra013814.1-P
Bra019219.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G13930.1	Bra013814.1-P	AT4G24560.1	23232097
AT4G13930.1	Bra019219.1-P	AT4G24560.1	23232097

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY69223	Canola	cytosol	98.51	98.51
Bra014001.1-P	Field mustard	cytosol	90.66	98.39
Bra022129.1-P	Field mustard	cytosol	29.51	97.89
AT4G13930.1	Thale cress	cytosol	93.42	93.42
Zm00001d040457_P001	Maize	mitochondrion	18.68	90.72
Solyc05g053810.2.1	Tomato	nucleus, plastid	90.02	90.02
PGSC0003DMT400040717	Potato	cytosol	90.02	90.02
VIT_05s0029g00310.t01	Wine grape	cytosol	89.17	89.17
PGSC0003DMT400011868	Potato	cytosol	88.53	88.53
Solyc12g098490.1.1	Tomato	extracellular, nucleus, unclear	88.32	88.32
KRH58850	Soybean	cytosol	88.96	87.47
GSMUA_Achr6P06520_001	Banana	cytosol	82.59	87.42
GSMUA_Achr9P01620_001	Banana	cytosol	81.1	87.21
GSMUA_Achr8P00310_001	Banana	cytosol	46.5	86.9
GSMUA_Achr9P07310_001	Banana	cytosol	73.89	85.93
EES08452	Sorghum	cytosol	85.77	85.77
Os11t0455800-01	Rice	plasma membrane	85.56	85.56
TraesCS2A01G493700.1	Wheat	plastid	84.93	84.93
TraesCS2D01G493600.1	Wheat	cytosol	84.93	84.93
TraesCS2B01G521700.1	Wheat	cytosol	84.71	84.71
Zm00001d049234_P001	Maize	plastid	85.77	76.81
KRH42751	Soybean	endoplasmic reticulum, nucleus	90.45	74.48
HORVU2Hr1G110110.2	Barley	mitochondrion, plastid	84.29	72.05
Bra011796.1-P	Field mustard	mitochondrion	59.66	54.35
Bra010622.1-P	Field mustard	mitochondrion	59.24	53.97
Bra040080.1-P	Field mustard	plastid	58.39	51.98
Bra016147.1-P	Field mustard	mitochondrion	57.54	51.82
Bra036774.1-P	Field mustard	cytosol	63.27	50.51
Bra016392.1-P	Field mustard	cytosol	61.57	50.17
Bra031358.1-P	Field mustard	cytosol	61.15	49.4
Bra003928.1-P	Field mustard	mitochondrion	57.75	24.44

Protein Annotations

KEGG:00260+2.1.2.1	KEGG:00460+2.1.2.1	KEGG:00630+2.1.2.1	KEGG:00670+2.1.2.1	KEGG:00680+2.1.2.1	MapMan:1.3.5
Gene3D:3.40.640.10	Gene3D:3.90.1150.10	MapMan:4.1.4.3.1	EnsemblPlantsGene:Bra032749	EnsemblPlants:Bra032749.1	EnsemblPlants:Bra032749.1-P
GO:GO:0003674	GO:GO:0003824	GO:GO:0004372	GO:GO:0005488	GO:GO:0006545	GO:GO:0006730
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016740	GO:GO:0019264
GO:GO:0030170	GO:GO:0035999	InterPro:IPR015421	InterPro:IPR015422	UniProt:M4EVB6	HAMAP:MF_00051
PFAM:PF00464	PIRSF:PIRSF000412	ScanProsite:PS00096	PANTHER:PTHR11680	PANTHER:PTHR11680:SF11	InterPro:PyrdxlP-dep_Trfase
InterPro:PyrdxlP-dep_Trfase_dom1	InterPro:PyrdxlP-dep_Trfase_major	SUPFAM:SSF53383	InterPro:Ser_HO-MeTrfase	InterPro:Ser_HO-MeTrfase_PLP_BS	UniParc:UPI0002543C26
SEG:seg	:	:	:	:	:

Description

AT4G13930 (E=1e-254) SHM4 | SHM4 (serine hydroxymethyltransferase 4); catalytic/ glycine hydroxymethyltransferase/ pyridoxal phosphate binding

Coordinates

chrA04:+:4955201..4957204

Molecular Weight (calculated)

51686.9 Da

IEP (calculated)

7.272

GRAVY (calculated)

-0.256

Length

471 amino acids

Sequence
(BLAST)

001: MDPVSAWGNT PLVTVDPEIH DLIEKEKRRQ CRGIELIDSE NFTSFAVIEA LGSALTNKYS EGMPGNRYYG GNEFIDQIEN LCQSRALEAF RLDSASWGVN
101: VQPYSGSPAN FAAYTALLQP HDRIMGLDLP SGGHLTHGYY TSGGKKISAT SIYFESLPYK VNFTTGYIDY DKLEEKAMDF RPKLLICGGS AYPRDWDYAR
201: LRAVADKVGA LLLCDMAHIS GLVAAQEAAN PFEYCDVVTT TTHKSLRGPR AGMIFYRKGP KPPKKGQPEG AVYDFEDKIN FAVFPALQGG PHNHQIGALA
301: VALKQANTPG FKVYAKQVKA NAVALANYLM GKGYSIVTGG TENHLVLWDL RPLGLTGNKV EKLCDLCNIT LNKNAVFGDS SALAPGGVRI GTPAMTSRGL
401: VEKDFEMIGE FLSRSVTLTL NIQKEHGKLL KDFNKGLVNN KEIEELKADV EKFSASYEMP GFLMSAMKYK D

Best Arabidopsis Sequence Match ( AT4G13930.1 )
(BLAST)

001: MEPVSSWGNT SLVSVDPEIH DLIEKEKRRQ CRGIELIASE NFTSFAVIEA LGSALTNKYS EGIPGNRYYG GNEFIDEIEN LCRSRALEAF HCDPAAWGVN
101: VQPYSGSPAN FAAYTALLQP HDRIMGLDLP SGGHLTHGYY TSGGKKISAT SIYFESLPYK VNFTTGYIDY DKLEEKALDF RPKLLICGGS AYPRDWDYAR
201: FRAIADKVGA LLLCDMAHIS GLVAAQEAAN PFEYCDVVTT TTHKSLRGPR AGMIFYRKGP KPPKKGQPEG AVYDFEDKIN FAVFPALQGG PHNHQIGALA
301: VALKQANTPG FKVYAKQVKA NAVALGNYLM SKGYQIVTNG TENHLVLWDL RPLGLTGNKV EKLCDLCSIT LNKNAVFGDS SALAPGGVRI GAPAMTSRGL
401: VEKDFEQIGE FLSRAVTLTL DIQKTYGKLL KDFNKGLVNN KDLDQLKADV EKFSASYEMP GFLMSEMKYK D

Arabidopsis Description

SHM4Serine hydroxymethyltransferase 4 [Source:UniProtKB/Swiss-Prot;Acc:O23254]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.