CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

nucleus 1
mitochondrion 2
cytosol 1
plastid 2
peroxisome 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH16837
KRH52893
KRH62182
KRH72452

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G13930.1	KRH16837	AT4G24560.1	23232097
AT4G13930.1	KRH52893	AT4G24560.1	23232097
AT4G13930.1	KRH62182	AT4G24560.1	23232097
AT4G13930.1	KRH72452	AT4G24560.1	23232097

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH58850	Soybean	cytosol	80.07	95.62
Solyc05g053810.2.1	Tomato	nucleus, plastid	76.75	93.21
PGSC0003DMT400040717	Potato	cytosol	76.22	92.57
VIT_05s0029g00310.t01	Wine grape	cytosol	75.87	92.14
PGSC0003DMT400011868	Potato	cytosol	74.83	90.87
CDY69223	Canola	cytosol	74.65	90.66
Solyc12g098490.1.1	Tomato	extracellular, nucleus, unclear	74.65	90.66
Bra032749.1-P	Field mustard	cytosol	74.48	90.45
CDY01173	Canola	cytosol	68.36	90.09
CDY50421	Canola	cytosol	68.36	90.09
Bra014001.1-P	Field mustard	cytosol	68.18	89.86
CDY47310	Canola	cytosol	68.18	89.86
AT4G13930.1	Thale cress	cytosol	73.43	89.17
GSMUA_Achr9P01620_001	Banana	cytosol	67.83	88.58
GSMUA_Achr6P06520_001	Banana	cytosol	68.01	87.42
GSMUA_Achr8P00310_001	Banana	cytosol	38.46	87.3
Zm00001d040457_P001	Maize	mitochondrion	14.69	86.6
GSMUA_Achr9P07310_001	Banana	cytosol	60.84	85.93
EES08452	Sorghum	cytosol	70.28	85.35
Os11t0455800-01	Rice	plasma membrane	69.93	84.93
TraesCS2D01G493600.1	Wheat	cytosol	69.41	84.29
TraesCS2A01G493700.1	Wheat	plastid	69.41	84.29
TraesCS2B01G521700.1	Wheat	cytosol	69.23	84.08
Zm00001d049234_P001	Maize	plastid	72.2	78.52
AT4G13890.1	Thale cress	cytosol	64.51	78.51
HORVU2Hr1G110110.2	Barley	mitochondrion, plastid	70.45	73.14
KRH11116	Soybean	cytosol	23.95	56.15
KRH53150	Soybean	cytosol, nucleus, peroxisome, plastid	53.67	56.02
KRH64759	Soybean	cytosol, peroxisome	10.14	55.77
KRH26369	Soybean	cytosol	53.85	54.71
KRH07754	Soybean	cytosol, nucleus, peroxisome	20.63	54.63
KRH16896	Soybean	mitochondrion	48.25	54.44
KRH26204	Soybean	cytosol	18.88	54.27
KRH44066	Soybean	plastid	53.67	54.24
KRH72501	Soybean	mitochondrion	48.6	54.09
KRG99502	Soybean	mitochondrion	48.43	53.47
KRH45493	Soybean	mitochondrion	48.25	53.28
KRH21130	Soybean	plastid	48.43	51.68
KRH39500	Soybean	mitochondrion	46.5	51.45
KRH64758	Soybean	cytosol	43.53	50.0
KRH11117	Soybean	plastid	18.36	46.26
KRH18830	Soybean	cytosol, peroxisome, plastid	9.44	41.86

Protein Annotations

KEGG:00260+2.1.2.1	KEGG:00460+2.1.2.1	KEGG:00630+2.1.2.1	KEGG:00670+2.1.2.1	KEGG:00680+2.1.2.1	MapMan:1.3.5
EntrezGene:100305380	Gene3D:3.40.640.10	Gene3D:3.90.1150.10	MapMan:4.1.4.3.1	UniProt:A0A0R0IUS1	EMBL:ACUP02004823
EnsemblPlantsGene:GLYMA_08G108900	GO:GO:0003674	GO:GO:0003824	GO:GO:0004372	GO:GO:0005488	GO:GO:0006545
GO:GO:0006730	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016740
GO:GO:0019264	GO:GO:0030170	GO:GO:0035999	InterPro:IPR015421	InterPro:IPR015422	EnsemblPlants:KRH42751
ProteinID:KRH42751	ProteinID:KRH42751.1	HAMAP:MF_00051	PFAM:PF00464	ScanProsite:PS00096	PANTHER:PTHR11680
PANTHER:PTHR11680:SF11	MetaCyc:PWY-1622	MetaCyc:PWY-181	MetaCyc:PWY-2161	MetaCyc:PWY-2201	MetaCyc:PWY-3661
MetaCyc:PWY-3661-1	MetaCyc:PWY-3841	MetaCyc:PWY-5497	InterPro:PyrdxlP-dep_Trfase	InterPro:PyrdxlP-dep_Trfase_dom1	InterPro:PyrdxlP-dep_Trfase_major
SUPFAM:SSF53383	InterPro:Ser_HO-MeTrfase	InterPro:Ser_HO-MeTrfase_PLP_BS	UniParc:UPI0006ED78E3	SEG:seg	:

Description

hypothetical protein

Coordinates

chr8:+:8357504..8362425

Molecular Weight (calculated)

63130.7 Da

IEP (calculated)

8.060

GRAVY (calculated)

-0.248

Length

572 amino acids

Sequence
(BLAST)

001: MAPMPNGRFK SSPTSTSEIM NYEIKMLPTF KNEHIRTHIS IPPQQTQQHS LFSLLAFRSS LTLIHSFPPF MDPVSVWGNT PLATVDPEIH DLIEKEKRRQ
101: CRGIELIASE NFTSFAVIEA LGSALTNKYS EGMPGNRYYG GNEYIDQIEN LCRSRALQAF HLDAQSWGVN VQPYSGSPAN FAAYTAVLNP HDRIMGLDLP
201: SGGHLTHGYY TSGGKKISAT SIYFESLPYK VNSTTGYIDY DRLEEKALDF RPKLIICGGS AYPRDWDYKR FREVADKCGA LLLCDMAHTS GLVAAQEVNS
301: PFEYCDIVTT TTHKSLRGPR AGMIFYRKGP KPPKKGQPEN AVYDFEDKIN FAVFPSLQGG PHNHQIGALA VALKQAASPG FKAYAKQVKA NAVALGKYLM
401: GKGYSLVTGG TENHLVLWDL RPLGLTGNIY RIGSLPSGFD LLQMSINLTC SLCDCFAGNK VEKLCDLCNI TVNKNAVFGD SSALAPGGVR IGAPAMTSRG
501: LVEKDFEQIG EFLHRAVTLT LEIQKEHGKL LKDFNKGLVN NKAIEDLKAD VEKFSALFDM PGFLVSEMKY KD

Best Arabidopsis Sequence Match ( AT4G13930.1 )
(BLAST)

001: MEPVSSWGNT SLVSVDPEIH DLIEKEKRRQ CRGIELIASE NFTSFAVIEA LGSALTNKYS EGIPGNRYYG GNEFIDEIEN LCRSRALEAF HCDPAAWGVN
101: VQPYSGSPAN FAAYTALLQP HDRIMGLDLP SGGHLTHGYY TSGGKKISAT SIYFESLPYK VNFTTGYIDY DKLEEKALDF RPKLLICGGS AYPRDWDYAR
201: FRAIADKVGA LLLCDMAHIS GLVAAQEAAN PFEYCDVVTT TTHKSLRGPR AGMIFYRKGP KPPKKGQPEG AVYDFEDKIN FAVFPALQGG PHNHQIGALA
301: VALKQANTPG FKVYAKQVKA NAVALGNYLM SKGYQIVTNG TENHLVLWDL RPLGLTGNKV EKLCDLCSIT LNKNAVFGDS SALAPGGVRI GAPAMTSRGL
401: VEKDFEQIGE FLSRAVTLTL DIQKTYGKLL KDFNKGLVNN KDLDQLKADV EKFSASYEMP GFLMSEMKYK D

Arabidopsis Description

SHM4Serine hydroxymethyltransferase 4 [Source:UniProtKB/Swiss-Prot;Acc:O23254]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.