CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

nucleus 1
mitochondrion 3
cytosol 3

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
mitochondrion: 21766870
mitochondrion: 27780359

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 21766870 doi

S Komatsu, A Yamamoto, T Nakamura, MZ Nouri, Y Nanjo, K Nishizawa, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH02973
KRH41490
KRH48988
KRH60208
KRH72370
KRH74246
KRH74248

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G01280.1	KRH41490	AT4G29130.1	17965176
AT3G01280.1	KRH48988	AT4G29130.1	17965176
AT3G01280.1	KRH60208	AT4G29130.1	17965176
AT3G01280.1	KRH74246	AT4G29130.1	17965176
AT3G01280.1	KRH74248	AT4G29130.1	17965176
AT3G01280.1	KRH02973	AT4G30490.1	19523112
AT3G01280.1	KRH72370	AT4G30490.1	19523112

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG93501	Soybean	endoplasmic reticulum, mitochondrion	96.38	96.38
KRH40133	Soybean	mitochondrion	90.58	90.58
KRH01127	Soybean	mitochondrion	89.49	89.49
VIT_14s0108g00010.t01	Wine grape	cytosol	81.16	81.16
PGSC0003DMT400005245	Potato	cytosol	80.8	80.8
PGSC0003DMT400017754	Potato	cytosol	80.07	80.07
Solyc02g067460.2.1	Tomato	plastid	80.07	80.07
CDY66736	Canola	cytosol, mitochondrion, nucleus	76.09	76.09
CDY68122	Canola	cytosol	75.36	75.36
Bra040507.1-P	Field mustard	cytosol	74.64	74.64
CDY28690	Canola	cytosol	74.64	74.64
CDX91952	Canola	mitochondrion	74.28	74.28
Bra006664.1-P	Field mustard	cytosol	74.28	74.28
AT3G01280.1	Thale cress	cytosol, mitochondrion	74.28	74.28
CDX74294	Canola	cytosol, mitochondrion	73.91	73.91
KRH45848	Soybean	mitochondrion	73.55	73.55
KRG99160	Soybean	mitochondrion	73.55	73.55
CDY63765	Canola	cytosol	73.55	73.55
Bra001010.1-P	Field mustard	mitochondrion	73.55	73.55
CDY59668	Canola	cytosol	73.55	73.55
CDY69321	Canola	cytosol	72.1	72.1
Bra008723.1-P	Field mustard	cytosol	72.1	72.1
CDY59666	Canola	cytosol	21.01	71.6
AT5G15090.2	Thale cress	cytosol, mitochondrion	70.29	70.8
Solyc03g058920.2.1	Tomato	plastid	80.07	65.38
Bra039131.1-P	Field mustard	cytosol, golgi, mitochondrion	75.0	63.5
CDY70638	Canola	cytosol, mitochondrion	49.28	54.4
KRH28358	Soybean	mitochondrion	53.26	53.26
KRH77123	Soybean	endoplasmic reticulum, mitochondrion	52.54	52.54
KRH15450	Soybean	cytosol	46.38	46.21
KRH02881	Soybean	mitochondrion	44.93	44.77
KRH05595	Soybean	plastid	40.58	44.62
KRH19015	Soybean	cytosol	43.84	43.68
KRH56957	Soybean	cytosol	38.77	43.67
KRH03426	Soybean	cytosol	38.04	42.86
KRH63406	Soybean	cytosol	35.51	39.52
KRH54504	Soybean	cytosol	34.78	38.71

Protein Annotations

EntrezGene:100802449	Gene3D:2.40.160.10	MapMan:24.4.1	EMBL:ACUP02008084	EnsemblPlantsGene:GLYMA_13G064900	GO:GO:0003674
GO:GO:0005215	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005739
GO:GO:0005741	GO:GO:0006810	GO:GO:0008150	GO:GO:0008308	GO:GO:0015698	GO:GO:0016020
GO:GO:0055085	GO:GO:0098656	UniProt:I1LWI5	InterPro:IPR023614	EnsemblPlants:KRH18503	ProteinID:KRH18503
ProteinID:KRH18503.1	PFAM:PF01459	ScanProsite:PS00558	PANTHER:PTHR11743	PANTHER:PTHR11743:SF31	InterPro:Porin_Euk
InterPro:Porin_Euk/Tom40	InterPro:Porin_dom_sf	UniParc:UPI0001BA86B9	:	:	:

Description

hypothetical protein

Coordinates

chr13:+:16435965..16438939

Molecular Weight (calculated)

29764.3 Da

IEP (calculated)

9.607

GRAVY (calculated)

-0.241

Length

276 amino acids

Sequence
(BLAST)

001: MSKGPGLYSD IGKKARDLLF KDYHSDQKFT ITTYSPTGVA ITSSGTKKGD LFVADVNTQL KNKNITTDIK LDTNSNLLTT ITVNEPAPGL KTIFSFRVPD
101: QRSGKVEVQY LHDYAGISTS VGLTANPIVN FSGVVGTNVL ALGTDVSFDT KIGELTKFNA GLNFTKDDLV ASLTVNDKGD ALNASYYHAV NRLTNTAVGA
201: EVTHRFSTNE NTLTLGTQHA LDPLTTMKAR VNNFGKANAL IQHEWRPKSF FTISGEVDTK AIEKSAKVGL GLALKP

Best Arabidopsis Sequence Match ( AT3G01280.1 )
(BLAST)

001: MVKGPGLYTE IGKKARDLLY KDHNSDQKFS ITTFSPAGVA ITSTGTKKGD LLLGDVAFQS RRKNITTDLK VCTDSTFLIT ATVDEAAPGL RSIFSFKVPD
101: QNSGKVELQY LHEYAGISTS MGLTQNPTVN FSGVIGSNVL AVGTDVSFDT KSGNFTKINA GLSFTKEDLI ASLTVNDKGD LLNASYYHIV NPLFNTAVGA
201: EVSHKLSSKD STITVGTQHS LDPLTSVKAR VNSAGIASAL IQHEWKPKSF FTISGEVDTK SIDKSAKVGL ALALKP

Arabidopsis Description

VDAC1Mitochondrial outer membrane protein porin 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9SRH5]
SUBAcon: [mitochondrion,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.