CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

nucleus 1
mitochondrion 4
cytosol 3

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
extracellular: 25724908
endoplasmic reticulum: 27224218
nucleus: 27291164
mitochondrion: 27780359
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 25724908 doi

VC Clarke, PC Loughlin, A Gavrin, C Chen, EM Brear, DA Day, PM Smith

From the ‡University of Sydney, School of Biological Sciences, Sydney Australia;, From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; penny.smith@sydney.edu.au., From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; §Flinders University, School of Biological Sciences, Adelaide Australia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH02973
KRH41490
KRH48988
KRH60208
KRH72370
KRH74246
KRH74248

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G01280.1	KRH41490	AT4G29130.1	17965176
AT3G01280.1	KRH48988	AT4G29130.1	17965176
AT3G01280.1	KRH60208	AT4G29130.1	17965176
AT3G01280.1	KRH74246	AT4G29130.1	17965176
AT3G01280.1	KRH74248	AT4G29130.1	17965176
AT3G01280.1	KRH02973	AT4G30490.1	19523112
AT3G01280.1	KRH72370	AT4G30490.1	19523112

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH01127	Soybean	mitochondrion	98.91	98.91
KRH18503	Soybean	mitochondrion	90.58	90.58
KRG93501	Soybean	endoplasmic reticulum, mitochondrion	89.49	89.49
VIT_14s0108g00010.t01	Wine grape	cytosol	80.07	80.07
PGSC0003DMT400005245	Potato	cytosol	78.26	78.26
Solyc02g067460.2.1	Tomato	plastid	76.81	76.81
PGSC0003DMT400017754	Potato	cytosol	76.81	76.81
Bra006664.1-P	Field mustard	cytosol	73.55	73.55
KRG99160	Soybean	mitochondrion	73.19	73.19
KRH45848	Soybean	mitochondrion	73.19	73.19
AT3G01280.1	Thale cress	cytosol, mitochondrion	73.19	73.19
CDY66736	Canola	cytosol, mitochondrion, nucleus	73.19	73.19
Bra040507.1-P	Field mustard	cytosol	73.19	73.19
CDY28690	Canola	cytosol	73.19	73.19
CDY68122	Canola	cytosol	72.83	72.83
CDY59668	Canola	cytosol	72.1	72.1
CDY63765	Canola	cytosol	72.1	72.1
CDX91952	Canola	mitochondrion	72.1	72.1
CDX74294	Canola	cytosol, mitochondrion	71.74	71.74
Bra001010.1-P	Field mustard	mitochondrion	71.38	71.38
Bra008723.1-P	Field mustard	cytosol	71.38	71.38
CDY69321	Canola	cytosol	71.38	71.38
AT5G15090.2	Thale cress	cytosol, mitochondrion	67.75	68.25
CDY59666	Canola	cytosol	19.57	66.67
Solyc03g058920.2.1	Tomato	plastid	77.17	63.02
Bra039131.1-P	Field mustard	cytosol, golgi, mitochondrion	73.19	61.96
CDY70638	Canola	cytosol, mitochondrion	49.64	54.8
KRH28358	Soybean	mitochondrion	50.72	50.72
KRH77123	Soybean	endoplasmic reticulum, mitochondrion	50.36	50.36
KRH02881	Soybean	mitochondrion	45.29	45.13
KRH15450	Soybean	cytosol	45.29	45.13
KRH19015	Soybean	cytosol	43.84	43.68
KRH05595	Soybean	plastid	39.13	43.03
KRH56957	Soybean	cytosol	36.59	41.22
KRH03426	Soybean	cytosol	35.87	40.41
KRH63406	Soybean	cytosol	36.23	40.32
KRH54504	Soybean	cytosol	35.14	39.11

Protein Annotations

Gene3D:2.40.160.10	MapMan:24.4.1	EMBL:ACUP02006021	EnsemblPlantsGene:GLYMA_09G240600	GO:GO:0003674	GO:GO:0005215
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005739	GO:GO:0005741
GO:GO:0006810	GO:GO:0008150	GO:GO:0008308	GO:GO:0015698	GO:GO:0016020	GO:GO:0055085
GO:GO:0098656	UniProt:I1L602	InterPro:IPR023614	EnsemblPlants:KRH40133	ProteinID:KRH40133	ProteinID:KRH40133.1
PFAM:PF01459	ScanProsite:PS00558	PANTHER:PTHR11743	PANTHER:PTHR11743:SF31	InterPro:Porin_Euk	InterPro:Porin_Euk/Tom40
InterPro:Porin_dom_sf	UniParc:UPI00023C9866	:	:	:	:

Description

hypothetical protein

Coordinates

chr9:+:46301351..46305832

Molecular Weight (calculated)

29757.3 Da

IEP (calculated)

9.164

GRAVY (calculated)

-0.188

Length

276 amino acids

Sequence
(BLAST)

001: MAKGPGLYSD IGKKARDLLF KDYHSDQKFT ITTYSPTGVA ITASGTRKSE LFVADVNTQL KNKNITTDIK VDTDSNLFTT ITVNEPAPGL KAIFSFKVPD
101: QRSGKVEVQY LHDYAGISTS VGLTANPIVN FSGVIGTNVL ALGADLSFDT KIGELTKSNA GLSFTKDDLI ASLTLNDKGD ALNAAYYHVV NPLTNTAVGA
201: EVTHRFSTNE NTLTLGTQHQ LDPLTTLKAR VNNFGRTSAL IQHEWRPKSF FTISGEVDTK AIEKSAKVGL GLVLKP

Best Arabidopsis Sequence Match ( AT3G01280.1 )
(BLAST)

001: MVKGPGLYTE IGKKARDLLY KDHNSDQKFS ITTFSPAGVA ITSTGTKKGD LLLGDVAFQS RRKNITTDLK VCTDSTFLIT ATVDEAAPGL RSIFSFKVPD
101: QNSGKVELQY LHEYAGISTS MGLTQNPTVN FSGVIGSNVL AVGTDVSFDT KSGNFTKINA GLSFTKEDLI ASLTVNDKGD LLNASYYHIV NPLFNTAVGA
201: EVSHKLSSKD STITVGTQHS LDPLTSVKAR VNSAGIASAL IQHEWKPKSF FTISGEVDTK SIDKSAKVGL ALALKP

Arabidopsis Description

VDAC1Mitochondrial outer membrane protein porin 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9SRH5]
SUBAcon: [mitochondrion,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.