CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 5
cytosol 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	nucleus
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	nucleus

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH18664
KRH31638
KRH69070

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G54610.1	KRG89812	AT2G41090.1	17360592
AT1G54610.1	KRG94975	AT2G41090.1	17360592
AT1G54610.1	KRH18664	AT2G41090.1	17360592
AT1G54610.1	KRG89812	AT2G41100.1	17360592
AT1G54610.1	KRG94975	AT2G41100.1	17360592
AT1G54610.1	KRH18664	AT2G41100.1	17360592
AT1G54610.1	KRH31638	AT4G14640.1	17360592
AT1G54610.1	KRH69070	AT4G14640.1	17360592
AT1G54610.1	KRG89812	AT5G21274.1	17360592
AT1G54610.1	KRG94975	AT5G21274.1	17360592
AT1G54610.1	KRH18664	AT5G21274.1	17360592
AT3G05050.1	KRH31638	AT4G14640.1	17360592
AT3G05050.1	KRH69070	AT4G14640.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG92786	Soybean	nucleus	94.31	94.31
Os07t0668000-00	Rice	cytosol, plasma membrane	4.66	87.1
KRG96918	Soybean	cytosol	82.93	84.09
KRH68662	Soybean	nucleus	84.83	83.11
KRH21021	Soybean	cytosol	68.79	73.89
PGSC0003DMT400057839	Potato	cytosol	73.1	73.61
Solyc01g098160.2.1	Tomato	nucleus	72.76	73.39
KRH11268	Soybean	cytosol	67.93	72.83
KRH02197	Soybean	nucleus	68.1	72.34
KRH50926	Soybean	nucleus	68.28	72.26
TraesCS2D01G120200.1	Wheat	nucleus	57.41	69.96
VIT_14s0030g01260.t01	Wine grape	cytosol, nucleus, plastid	70.0	69.76
Bra027966.1-P	Field mustard	nucleus	66.55	68.56
CDY67158	Canola	plastid	66.72	68.37
CDX95237	Canola	nucleus	67.24	68.3
Zm00001d047482_P001	Maize	nucleus	63.79	67.52
AT1G54610.1	Thale cress	nucleus	66.21	67.13
TraesCS4B01G151800.1	Wheat	nucleus	62.93	66.61
OQU92600	Sorghum	nucleus	64.48	66.31
Zm00001d029143_P003	Maize	nucleus	62.41	66.18
TraesCS4D01G151100.1	Wheat	nucleus	62.76	65.7
Zm00001d022499_P001	Maize	plastid	64.66	65.67
KXG36890	Sorghum	plastid	64.48	65.38
TraesCS2A01G119300.1	Wheat	nucleus	63.62	65.19
TraesCS4A01G142300.1	Wheat	nucleus	62.07	64.98
Zm00001d007111_P001	Maize	plastid	64.14	64.92
TraesCS2B01G139000.1	Wheat	nucleus	63.45	64.45
KRH63826	Soybean	cytosol	39.14	62.19
KRH54127	Soybean	mitochondrion	59.14	61.36
HORVU4Hr1G048510.1	Barley	cytosol	56.9	59.57
Bra009977.1-P	Field mustard	cytosol, nucleus, plastid	57.59	59.12
CDX86178	Canola	cytosol, nucleus, plastid	57.59	59.12
CDX80672	Canola	cytosol, plastid	58.1	58.92
KRH59775	Soybean	nucleus	56.21	58.74
CDY18783	Canola	plastid	58.1	58.3
Bra040637.1-P	Field mustard	plastid	58.1	58.3
KRH41090	Soybean	cytosol	55.69	58.2
CDY53230	Canola	nucleus, plastid	58.45	58.05
AT3G05050.1	Thale cress	nucleus, plastid	59.31	58.01
HORVU2Hr1G020680.2	Barley	cytosol	48.97	57.84
CDY41930	Canola	cytosol	58.45	57.75
Bra039466.1-P	Field mustard	plastid	57.59	57.29
CDY27801	Canola	cytosol	57.59	57.29
KRH27243	Soybean	nucleus	56.21	46.05
KRH22087	Soybean	nucleus	56.38	45.93
KRH27807	Soybean	nucleus	49.31	45.91
KRH55283	Soybean	nucleus	55.86	45.63
KRH77684	Soybean	nucleus	48.62	44.48
KRH06043	Soybean	nucleus	44.31	44.23
KRH53817	Soybean	nucleus	37.41	44.11
KRH26151	Soybean	nucleus	54.83	43.5
KRG91692	Soybean	endoplasmic reticulum, plasma membrane	35.17	43.13
KRH59494	Soybean	nucleus	30.86	42.93
KRH64126	Soybean	nucleus	36.9	42.8
KRH63286	Soybean	nucleus	49.14	42.28
KRH54601	Soybean	plastid	49.31	42.12
KRH56877	Soybean	nucleus, plastid	48.45	41.94
KRG93581	Soybean	plastid	42.24	41.81
KRH18441	Soybean	nucleus	42.07	41.57
KRH26282	Soybean	nucleus	50.52	41.33
KRH03527	Soybean	nucleus, plastid	48.1	41.09
KRH26956	Soybean	nucleus	48.62	40.52
KRH55951	Soybean	nucleus	48.28	40.23
KRH22379	Soybean	nucleus	48.28	39.83
KRH25594	Soybean	nucleus	47.41	39.57
KRH14656	Soybean	nucleus	33.79	37.98
KRH73489	Soybean	nucleus	33.62	37.79
KRG89779	Soybean	nucleus	33.62	37.5
KRH35103	Soybean	nucleus	38.62	37.15
KRH44946	Soybean	nucleus	43.1	36.87
KRH01235	Soybean	nucleus, plastid	42.76	36.85

Protein Annotations

Gene3D:1.10.510.10	EntrezGene:100817289	MapMan:18.4.3.1.8	Gene3D:3.30.200.20	EMBL:ACUP02006571	EnsemblPlantsGene:GLYMA_10G158800
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004693	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0006464
GO:GO:0006468	GO:GO:0007049	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301
GO:GO:0016740	GO:GO:0019538	GO:GO:0051726	UniProt:I1LBG8	InterPro:IPR000719	ProteinID:KRH34012.1
EnsemblPlants:KRH34013	ProteinID:KRH34013	ProteinID:KRH34013.1	ProteinID:KRH34014.1	ProteinID:KRH34015.1	ProteinID:KRH34016.1
ProteinID:KRH34017.1	ProteinID:KRH34018.1	ProteinID:KRH34019.1	InterPro:Kinase-like_dom_sf	PFAM:PF00069	ScanProsite:PS00107
ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24056	PANTHER:PTHR24056:SF176	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS
SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI000233CAD7	SEG:seg	:

Description

hypothetical protein

Coordinates

chr10:-:39283048..39289660

Molecular Weight (calculated)

64262.8 Da

IEP (calculated)

9.732

GRAVY (calculated)

-0.567

Length

580 amino acids

Sequence
(BLAST)

001: MGCVISREVS SGIVSEVKEE KNLSVGSNKK VDEASTSGAE ENAVVAQNGE KEKEENGGGD DRVRRPKGER RRSKPNPRLS NPPKHLQGEQ VAAGWPPWLT
101: AVCGEALSGW IPRKADTFEK IDKIGQGTYS NVYKAKDTLT GKIVALKKVR FDNLEPESVK FMAREILILR RLDHPNVIKL EGLVTSRMSL SLYLVFDYMV
201: HDLAGLAASP DIKFTEPQVK CYIHQLLSGL EHCHSRNVLH RDIKGSNLLI DNEGILKIAD FGLASFFDPN RRQPMTNRVV TLWYRPLELL LGATEYGAAI
301: DLWSVGCILG ELLAGKPILP GRTEVEQLHK IYKLCGSPSD EYWKKSKMPN ATLFKPRHPY KRCITETFKD FPPSALPLID TLLAIDPAER KSATDALRSE
401: FFTTEPYACD PSSLPKYPPT KEMDAKRRDD EARRSRAAGK AHVDGAKKHR TRDRAAKAAP APEGNAELQS NIDRRRLITH ANAKSKSEKF PPPHEDGQLG
501: FPLGSSNHID PDIVPSDVSF GSTSYTFSKE PFQAWSGPIG NTASISVTKR KKHTAGDALD LSKPYKGALK DKVKGKKIIA

Best Arabidopsis Sequence Match ( AT1G54610.1 )
(BLAST)

001: MGCVFGREAA TTTTAEAKQA KSSKASSGVV VVGESSVTKS NGVIADDVEK KKNEEANGDK ERKSSKGDRR RSTKPNPRLS NPSKHWRGEQ VAAGWPSWLS
101: DACGEALNGW VPRKADTFEK IDKIGQGTYS NVYKAKDMLT GKIVALKKVR FDNLEPESVK FMAREILVLR RLDHPNVVKL EGLVTSRMSC SLYLVFQYMD
201: HDLAGLASSP VVKFSESEVK CLMRQLISGL EHCHSRGVLH RDIKGSNLLI DDGGVLKIAD FGLATIFDPN HKRPMTSRVV TLWYRAPELL LGATDYGVGI
301: DLWSAGCILA ELLAGRPIMP GRTEVEQLHK IYKLCGSPSE DYWKKGKFTH GAIYKPREPY KRSIRETFKD FPPSSLPLID ALLSIEPEDR QTASAALKSE
401: FFTSEPYACE PADLPKYPPS KEIDAKRRDE ETRRQRAASK AQGDGARKNR HRDRSNRALP APEANAELQS NVDRRRLITH ANAKSKSEKF PPPHQDGGAM
501: GVPLGASQHI DPTFIPRDMV PSFTSSSFNF SKDEPPTQVQ TWSGPLGHPI TGVSRKKKDN TKSSKGKRAV VA

Arabidopsis Description

Probable serine/threonine-protein kinase At1g54610 [Source:UniProtKB/Swiss-Prot;Acc:Q9ZVM9]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.