Factsheet for PGSC0003DMT400017962

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, peroxisome, vacuole

Predictor Summary:

nucleus 1
vacuole 2
plasma membrane 3
extracellular 1
golgi 1
endoplasmic reticulum 1
peroxisome 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	peroxisome, plasma membrane, vacuole
Any Predictor:	nucleus, peroxisome, secretory
BaCelLo:	nucleus
MultiLoc:	vacuole
Plant-mPloc:	plasma membrane
PProwler:	secretory
WoLF PSORT:	plasma membrane
YLoc:	peroxisome

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400006495
PGSC0003DMT400071510
PGSC0003DMT400073246
PGSC0003DMT400084737

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G40890.1	PGSC0003DMT400084737	AT1G27300.1	21798944
AT5G40890.1	PGSC0003DMT400006495	AT3G16310.1	21798944
AT5G40890.1	PGSC0003DMT400071510	AT4G19030.1	21798944
AT5G40890.1	PGSC0003DMT400073246	AT4G19030.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc02g068080.2.1	Tomato	peroxisome, plasma membrane, vacuole	98.98	98.98
VIT_14s0068g02190.t01	Wine grape	plasma membrane	82.65	82.13
PGSC0003DMT400052072	Potato	plasma membrane	80.23	81.27
KRG94508	Soybean	plasma membrane	79.72	79.11
KRH57669	Soybean	plasma membrane	77.93	78.64
Bra028478.1-P	Field mustard	plasma membrane	76.79	78.18
CDY11722	Canola	plasma membrane	76.79	78.18
KRH06955	Soybean	plasma membrane	74.23	78.12
CDX67478	Canola	plasma membrane	76.66	78.05
Bra030328.1-P	Field mustard	plasma membrane	76.91	77.71
CDY15925	Canola	plasma membrane	76.79	77.58
AT3G27170.1	Thale cress	plasma membrane	77.17	77.56
CDX83663	Canola	plasma membrane	77.3	77.39
CDX99630	Canola	plasma membrane	76.4	77.19
CDY34173	Canola	plasma membrane	76.79	77.18
Bra025253.1-P	Field mustard	plasma membrane	76.91	77.01
AT5G40890.1	Thale cress	plasma membrane	76.02	76.9
CDY13685	Canola	plasma membrane	76.79	76.88
Bra032985.1-P	Field mustard	plasma membrane	76.66	76.76
CDY28488	Canola	plasma membrane	76.4	76.5
GSMUA_Achr3P16100_001	Banana	plasma membrane	68.37	73.93
VIT_14s0068g02330.t01	Wine grape	cytosol, extracellular	7.65	73.17
TraesCS6D01G084300.1	Wheat	cytosol, nucleus, plasma membrane	69.52	69.25
TraesCS6B01G126400.1	Wheat	plasma membrane	69.39	69.12
TraesCS6B01G126800.1	Wheat	plasma membrane	68.75	68.75
TraesCS6D01G084000.2	Wheat	plasma membrane	68.37	68.37
TraesCS6A01G098600.1	Wheat	plasma membrane	67.73	68.25
VIT_00s0316g00020.t01	Wine grape	cytosol	7.27	67.86
Zm00001d046919_P001	Maize	plasma membrane	68.75	67.29
EER90148	Sorghum	plasma membrane	68.75	67.29
TraesCS6A01G098500.2	Wheat	cytosol, peroxisome, plasma membrane	67.35	66.33
HORVU0Hr1G015220.3	Barley	cytosol	30.61	58.39
Os12t0438600-01	Rice	plasma membrane	10.46	53.95
PGSC0003DMT400078919	Potato	plasma membrane	52.17	53.32
PGSC0003DMT400025239	Potato	plasma membrane	52.42	52.96
PGSC0003DMT400064087	Potato	plasma membrane	52.3	52.9

Protein Annotations

Gene3D:1.10.3080.10	EntrezGene:102601748	MapMan:24.2.16	Gene3D:3.10.580.10	InterPro:CBS_dom	InterPro:Cl-channel_core
InterPro:Cl-channel_volt-gated	InterPro:Cl_channel_pln	GO:GO:0003674	GO:GO:0005215	GO:GO:0005216	GO:GO:0005247
GO:GO:0005575	GO:GO:0006810	GO:GO:0006811	GO:GO:0006821	GO:GO:0008150	GO:GO:0016020
GO:GO:0016021	GO:GO:0034220	GO:GO:0055085	GO:GO:1902476	InterPro:IPR000644	InterPro:IPR014743
UniProt:M1A9T9	PFAM:PF00654	EnsemblPlantsGene:PGSC0003DMG400006975	PGSC:PGSC0003DMG400006975	EnsemblPlants:PGSC0003DMT400017962	PRINTS:PR00762
PRINTS:PR01120	PFscan:PS51371	PANTHER:PTHR11689	PANTHER:PTHR11689:SF67	SMART:SM00116	SUPFAM:SSF54631
SUPFAM:SSF81340	TMHMM:TMhelix	UniParc:UPI0002961AD9	RefSeq:XP_006357190.1	SEG:seg	:

Description

CLC-Nt2 protein [Source:PGSC_GENE;Acc:PGSC0003DMG400006975]

Coordinates

chr2:-:26743487..26748156

Molecular Weight (calculated)

86452.4 Da

IEP (calculated)

8.637

GRAVY (calculated)

0.312

Length

784 amino acids

Sequence
(BLAST)

001: MEEQRLVEEA TTNDINGQLN EEERDPESNS MRQPLLKRNR TLSSSPFALV GAKVSHIESL DYEINENDLF KHDWRRRSRV QVLQYVFLKW SLAFLVGLLT
101: GVTATLINLA IENIAGYKLR AVVDYIDNRR YLMGFAYFAG ANFVLTLIAA LLCVCFAPTA AGPGIPEIKA YLNGVDTPNM FGATTLFVKI IGSIAAVSAS
201: LDLGKEGPLV HIGACFASLL GQGGPDNYRL KWRWLRYFNN DRDRRDLITS GSSSGVCAAF RSPVGGVLFA LEEVATWWRS ALLWRTFFST AVVVVILRAF
301: IEYCKSGNCG LFGRGGLIMF DVSGVSVTYH PVDIIPIALI GIIGGLLGSL YNHVLHKVLR LYNLINEKGK LHKLLLALSV SLFTSIGMYG LPFLAKCKPC
401: DPSIQGSCPG TGGTGNFKQF NCPNGYYNDL ATLLLTTNDD AVRNIFSINT PGEFQVSSLI IYFVLYCILG LVTFGIAVPS GLFLPIILMG SAYGRLLAIA
501: MGSYTKIDPG LYAVLGAASL MAGSMRMTVS LCVIFLELTN NLLLLPITML VLLISKSVGD CFNLSIYEII LELKGLPFLD ANPEPWMRNI TVGELADVKP
601: PVVTLRGIEK VGRIVEVLKN TTHNGFPVVD EGVVPPVGLP IGATELHGIV LRTHLLLVLK KKLFLHERRR TEEWEVREKF SWIDLAERWG KIEDVAVTKS
701: EMEMYVDLHP LTNTTPYTVV ESMSVAKAMV LFRQVGLRHM LIVPKYQAAG VSPVVGILTR QDLRAHNILS VFPHLVKSKS GKKH

Best Arabidopsis Sequence Match ( AT3G27170.1 )
(BLAST)

001: MVEEDLNQIG GNSNYNGEGG DPESNTLNQP LVKANRTLSS TPLALVGAKV SHIESLDYEI NENDLFKHDW RKRSKAQVLQ YVFLKWTLAC LVGLFTGLIA
101: TLINLAVENI AGYKLLAVGH FLTQERYVTG LMVLVGANLG LTLVASVLCV CFAPTAAGPG IPEIKAYLNG VDTPNMFGAT TMIVKIVGSI GAVAAGLDLG
201: KEGPLVHIGS CIASLLGQGG TDNHRIKWRW LRYFNNDRDR RDLITCGSAA GVCAAFRSPV GGVLFALEEV ATWWRSALLW RTFFSTAVVV VVLREFIEIC
301: NSGKCGLFGK GGLIMFDVSH VTYTYHVTDI IPVMLIGVIG GILGSLYNHL LHKVLRLYNL INEKGKIHKV LLSLTVSLFT SVCLYGLPFL AKCKPCDPSI
401: DEICPTNGRS GNFKQFHCPK GYYNDLATLL LTTNDDAVRN LFSSNTPNEF GMGSLWIFFV LYCILGLFTF GIATPSGLFL PIILMGAAYG RMLGAAMGSY
501: TSIDQGLYAV LGAAALMAGS MRMTVSLCVI FLELTNNLLL LPITMIVLLI AKTVGDSFNP SIYDIILHLK GLPFLEANPE PWMRNLTVGE LGDAKPPVVT
601: LQGVEKVSNI VDVLKNTTHN AFPVLDEAEV PQVGLATGAT ELHGLILRAH LVKVLKKRWF LTEKRRTEEW EVREKFPWDE LAEREDNFDD VAITSAEMEM
701: YVDLHPLTNT TPYTVMENMS VAKALVLFRQ VGLRHLLIVP KIQASGMCPV VGILTRQDLR AYNILQAFPL LEKSKGGKTH

Arabidopsis Description

CLC-BChloride channel protein [Source:UniProtKB/TrEMBL;Acc:A0A178VKG6]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.